[Study on the development of public health and safety literacy assessment scale for university freshmen].

Objective: To develop a measurement scale for the public health and safety literacy of university freshmen. Methods: Item pooling was conducted through literature review and in-depth interview with public health experts, and a nominal group was organized to develop the initial items to form the questionnaire. A total of 250 freshmen were randomly selected to complete the questionnaire, and the assessment of psychometric properties were performed to carry out item reduction by using classical test theory and item response theory. Exploratory factor analysis method (EFA) was employed to calculate the item-factor loadings. The data collected from another 318 freshmen were analyzed using confirmatory factor analysis method (CFA). Results: The scale contained 3 dimensions and 9 items with all content validity index ≥0.8. The difficulty coefficient of 9 items was 1.18-2.43. The discriminative validity analysis showed that the difficulty index was 0.41-0.78, and the discrimination index was 0.38-0.64. Each of the remaining items had a statistically significant difference in the score value between the top 27% and lowest 27% of respondents (t>3, P<0.05). The item's Cronbach's α was 0.72-0.77, corrected item-total correlation was 0.33-0.60 and the overall α of the scale was 0.77. EFA indicated all item-factor loading were higher than 0.680, and the accumulated variance contribution of the 3 factors was 62.484%. Results of CFA showed that the overall fitness index of the model reached the fitness standard except for the χ2 value. There was no statistical significance in the total score of the scale and the scores of each item among groups with different demographic characteristics (P>0.05). Conclusions: The newly developed public health safety literacy scale had a good reliability,validity,stability and item constancy.It can be used in measurement of the public health and safety literacy of university freshmen.

An endodermal sinus tumor in the cerebellopontine angle.

Immunohistochemical and ultrastructural findings in a primary intracranial endodermal sinus tumor are reported in this paper. The tumor cells exhibited AFP, CEA and anti-alpha-1-trypsin positive immunoreactivity immunocytochemically. Aggregates of electron-dense material in the extra- and intracellular spaces and amorphous basement membrane-like substance were seen extracellularly by electron microscopy. The clinicopathological, immunocytochemical and ultrastructural features were consistent with the criteria for primary intracranial sinus tumor.

Mother-and-child cases of sporotrichosis infection.

We report the appearance of lymphocutaneous sporotrichosis on the left arm of a 28-year-old farmer's wife, followed two months later by the same type of lesion on the right upper eyelid of this patient's three-year-old child. According to the mother, the child was accustomed to sleeping with the right side of her face applied to the lesion on the mother's arm, and so it was presumed that the infection had been transmitted by direct contact from mother to child. Cases of person-to-person contagion of this disorder are thought to be extremely rare, but where there has been close contact on a daily basis, such contagion is likely to occur.

[Effect of oxymatrine on beating of cultured myocardial cells in vitro].

Oxymatrine (OM), extracted from Sophora alopecuroides L, increased the contractility of the left heart atrium and decreased the spontaneous beating rate of right atrium of rabbit as described in our previous paper. The effect of OM on the beating rate of cultured rat myocardial cells was observed in the present study by a method of recording photoelectric energy transformation. OM 50 mumol/L decreased the beating rate of the myocytes by 50 +/- 27% 15 min after administration and also antagonized the positive chronotropic effect of isoproterenol 1.5 mumol/L, while the negative chronotropic effect of OM was completely abolished by prazosin. However, OM 250 mumol/L accelerated the beating rate of myocytes, which was cancelled by propranolol. These results suggest that OM has biphasic effects on cultured rat myocytes: slowing rate by alpha-adrenoceptor stimulation with 50 mumol/L concentration and accelerating rate by beta-adrenoceptor stimulation with 250 mumol/L concentration.

Prevention of postischemic reperfusion damage on isolated working rat hearts by bopindolol and propranolol.

Reperfusion after 30-min regional or global ischemia of the isolated working rat hearts did not restore the cardiac functions (as measured by cardiac output and power production), but exacerbated the existing damages. The lipid peroxidation product malondialdehyde (MDA) in the regional and global ischemic-reperfused myocardium increased by 37.6 and 45.2%, respectively. Bopindolol 0.1 mumol/L and propranolol 10 mumols/L protected the myocardium against the postischemic reperfusion damages, accelerated the recovery of cardiac functions during reperfusion and decreased the MDA content in the ischemic-reperfused myocardium. It is postulated that the prevention of cardiac cells from lipid peroxidation injury is related to the protection afforded by the drugs to the ischemic-reperfused hearts.

Synthesis and channel properties of [Tau 16]gramicidin A.

Des(ethanolamine)-taurine16-gramicidin A ([Tau 16]gramicidin A) was synthesized by the solid phase method and its channel-forming behavior in planar lipid bilayers was examined. The purified monovalent anionic peptide formed channels when applied to the aqueous compartments on both sides of the bilayer, but not when applied to one side only. The single-channel conductance was measured for KCl concentrations between 0.1 and 1.0 M and was found to be higher than that of gramicidin A in each case. Single-channel lifetimes were similar to those of gramicidin A suggesting that the channels have the beta 6.3 helix structure.