RESUMEN
To improve our understanding of glaucoma pathophysiology it is important to investigate endophenotypes which are determined by heritable quantitative traits, such as intraocular pressure, vertical cup-to-disc ratio, optic disc area, and central corneal thickness. Glaucoma is associated with increased intraocular pressure and defects of trabecular meshwork and anterior chamber which lead to derangement of intraocular fluid outflow, progressive optic nerve degeneration, loss of nerve cells, and blindness. Factors that are not affected by intraocular pressure also contribute to glaucoma degeneration. In progressive degenerative diseases, such as glaucoma, chronic astrocyte activation exacerbates the damage to neurons and impairs the regeneration of their axons. This review considers the polymorphism of predisposition factors to glaucoma-associated endophenotypes, optic nerve degeneration, and side effects of antiglaucomatous drugs.
Asunto(s)
Predisposición Genética a la Enfermedad , Glaucoma/genética , Polimorfismo Genético , Humanos , Presión Intraocular/genéticaRESUMEN
Visual loss due to age-related macular degeneration (AMD) is caused by one or both forms of advanced disease: "wet" (neovascular) or "dry" (geographic atrophy). Immune system plays a central role in pathogenesis and progression of both AMD forms. Main genetic polymorphisms associated with risk of AMD development and progression were found to be genes that regulate inflammation especially in complement factor H gen (1q31 locus) and 10q26 locus (PLEKHAI/ARMS2/HTRA1). Association of response to treatment and genotype was shown in patients with AMD. Complete characterization of both common and rare alleles that influence AMD risk is necessary for accurate determination of individual genetic risk as well as identification of new targets for therapeutic intervention.
Asunto(s)
Atrofia Geográfica , Glaucoma Neovascular , Degeneración Macular , Farmacogenética/métodos , Polimorfismo Genético , Factor H de Complemento/genética , Vía Alternativa del Complemento/genética , Progresión de la Enfermedad , Predisposición Genética a la Enfermedad , Atrofia Geográfica/complicaciones , Atrofia Geográfica/inmunología , Glaucoma Neovascular/complicaciones , Glaucoma Neovascular/inmunología , Humanos , Degeneración Macular/etiología , Degeneración Macular/genética , Degeneración Macular/fisiopatología , Degeneración Macular/terapia , Mutación , Medicina de PrecisiónAsunto(s)
Biomarcadores de Tumor/análisis , Linfoma de Células B Grandes Difuso/química , Linfoma de Células B Grandes Difuso/diagnóstico , Humanos , Factores Reguladores del Interferón/análisis , Linfoma de Células B Grandes Difuso/clasificación , Neoplasias del Mediastino/química , Neoplasias del Mediastino/diagnóstico , Neprilisina/análisis , Valor Predictivo de las Pruebas , Pronóstico , Proteínas Proto-Oncogénicas c-bcl-6/análisisAsunto(s)
Biomarcadores de Tumor/metabolismo , Leucemia Linfocítica Crónica de Células B/metabolismo , Proteína Tirosina Quinasa ZAP-70/metabolismo , Antineoplásicos/uso terapéutico , Linfocitos B/metabolismo , Regulación Neoplásica de la Expresión Génica , Humanos , Leucemia Linfocítica Crónica de Células B/terapia , Linfoma/metabolismo , Valor Predictivo de las Pruebas , Pronóstico , Transducción de Señal/efectos de los fármacos , Linfocitos T/metabolismoAsunto(s)
Antineoplásicos/farmacología , Neoplasias/metabolismo , Neoplasias/terapia , Receptores de Factores de Crecimiento/efectos de los fármacos , Transducción de Señal/efectos de los fármacos , Factores de Transcripción/efectos de los fármacos , Animales , Anticuerpos Monoclonales/farmacología , Antineoplásicos/uso terapéutico , Humanos , Neoplasias/genética , Oligonucleótidos Antisentido/farmacologíaAsunto(s)
Leucemia/terapia , Linfoma no Hodgkin/terapia , Animales , Trasplante de Médula Ósea , Humanos , Leucemia/metabolismo , Leucemia/patología , Leucemia Mielógena Crónica BCR-ABL Positiva/terapia , Leucemia Promielocítica Aguda/terapia , Linfoma Folicular/terapia , Linfoma de Células del Manto/terapia , Linfoma no Hodgkin/metabolismo , Linfoma no Hodgkin/patología , Mieloma Múltiple/terapia , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapia , Inducción de RemisiónRESUMEN
A double-species ecologo-genetical model, including Drosophila and yeast, has been used as a new methodological instrument for investigation of the physiological mechanism of recombination. Incubation of Drosophila females in the medium containing yeast of the strain mutant for ergosterol synthesis leads to suppression of temperature-induced crossing over. The mass-spectrum analysis of steroid fraction from Drosophila females has shown that incubation of the yeast medium without ergosterol results in arrest of ecdysterone synthesis. These data are explained by the absence of ecdysterone synthesis precursor in the fly organism. The endocrinal control of crossing over is discussed in the light of hormonal regulation of meiosis.
Asunto(s)
Intercambio Genético/genética , Drosophila/genética , Ecdisterona/biosíntesis , Recombinación Genética/genética , Inanición/genética , Esteroles/metabolismo , Animales , Femenino , Calor , Espectrometría de Masas , Modelos Genéticos , Saccharomyces cerevisiae/genética , Inanición/metabolismo , Supresión Genética/genéticaRESUMEN
The absence of sterols available for metabolism causes the death of Drosophila larva. Addition of suboptimal cholesterol doses to this medium allows the portion of larvae to survive. Sterol-deficient diet at the preimaginal stages leads to suppression of both spontaneous and high-temperature induced crossingover in Drosophila females. Two possible explanations for dependence of recombination process on sterol metabolism are suggested: 1) the shortage of precursor for ecdisons biosynthesis was the cause of discordance of meiotic events; 2) suppression of crossingover occurs, due to alteration of cell membrans' structure.
Asunto(s)
Intercambio Genético , Drosophila melanogaster/genética , Saccharomyces cerevisiae/metabolismo , Esteroles/metabolismo , Animales , Medios de Cultivo , Drosophila melanogaster/fisiología , Ecología , Saccharomyces cerevisiae/genéticaRESUMEN
The NYS1 gene of Saccharomyces cerevisiae yeasts is linked to the centromere marker of chromosome IV - gene TRP1 and is located at 16.2 cM distance from it.
Asunto(s)
Genes Fúngicos , Saccharomyces cerevisiae/genética , Mapeo Cromosómico , Cromosomas Fúngicos , Marcadores GenéticosRESUMEN
The strains of Saccharomyces cerevisiae yeast with mutations in two genes NYS3 NYS4 were obtained by tetrad analysis. Sterol fraction of these mutants contains two sterols: ergosta-7-en-3 beta-ol (fungisterol) and ergosta-7,24-dien-3 beta-ol (episterol). The findings allowed to testify the sequence of the ergosterol biosynthesis reactions. Dehydrogenization of the sterol nucleus in C5(6) which is controlled by gene NYS3 occurs simultaneously with the introduction of double bond in C22(23) site of the side chain regulated by gene NYS4.
Asunto(s)
Antibacterianos/farmacología , Saccharomyces cerevisiae/efectos de los fármacos , Farmacorresistencia Microbiana , Genes Fúngicos , Mutación , Polienos/farmacología , Saccharomyces cerevisiae/genéticaRESUMEN
A scheme of ergosterol cyclic intermediate transformations in S. cerevisiae has been proposed. It is based on the analysis of sterol composition in strains with mutant blocks of one or two reactions of enzymatic synthesis. Biochemical reactions of modification of cyclic part and side branch of sterol molecule are supposed to be carried out independently.
Asunto(s)
Sistema Enzimático del Citocromo P-450 , Ergosterol/biosíntesis , Saccharomyces cerevisiae/enzimología , Adenina/análogos & derivados , Adenina/farmacología , Farmacorresistencia Microbiana , Ergosterol/genética , Ergosterol/efectos de la radiación , Genes Fúngicos/efectos de los fármacos , Genes Fúngicos/efectos de la radiación , Metiltransferasas/metabolismo , Mutación , Nistatina/antagonistas & inhibidores , Oxidorreductasas/metabolismo , Oxidorreductasas N-Desmetilantes/metabolismo , Saccharomyces cerevisiae/efectos de los fármacos , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/efectos de la radiación , Esterol 14-Desmetilasa , Rayos UltravioletaRESUMEN
Nystatin-resistant strains of Saccharomyces cerevisiae with mutations in final steps of ergosterol biosynthesis have been studied in the ecologo-genetic yeast--drosophila system. It has been shown that yeast strains which belong to the Petersghoff genetic yeast stock collection, with mutations in NYSX, NYS2 and NYS3 genes, provide the development of Drosophila melanogaster. In the process of nutrition with yeasts having mutations in the NYS2 gene, the development of drosophila larvae takes place, due to ergosterol accumulated in the yeast cells. Drosophila melanogaster was shown to be unable to utilize the sterols with 8(9) and 24(25) double bonds.
Asunto(s)
Drosophila melanogaster/genética , Mutación , Nistatina/farmacología , Saccharomyces cerevisiae/genética , Animales , Drosophila melanogaster/fisiología , Farmacorresistencia Microbiana , Ecología , Genes Fúngicos , Saccharomyces cerevisiae/fisiología , Esteroles/biosíntesisRESUMEN
The comparative analysis of sterol content in the yeast Saccharomyces cerevisiae strains singly or doubly defective in nystatin resistance genes was carried out. The strains with two mutations in NYS genes were shown to accumulate the sterol mixture, similar to that of the parental singly defective mutant. This type of gene interaction allows to define the main biochemical order of reaction in ergosterol synthesis: methylation in C24 (NYS1), delta 8----delta 7 isomerization (NYS2), C5 (6) and C22 (23) desaturation (NYS3 and NYSX).