RESUMEN
The embryonic stage of development is defined as the period between fertilization and the establishment of most of the organ systems by the end of this period. Development in this stage is rapid. In many mammalian species, particularly in humans, the interval between fertilization and implantation is exactly determined and continuous without intermission. However, European roe deer (Capreolus capreolus) embryos undergo a reversible retardation of development. This interesting reproduction strategy is called embryonic diapause (delayed implantation). After this period of embryonic arrest, development continues without further interruption. The aim of this study was to investigate embryonic development after diapause in European roe deer. Because of the embryonic diapause and the unknown date of fertilization, it was impossible to assign the embryos to a certain gestational age (days). This study describes normal stages of embryonic development mainly based on the external morphological traits of 56 well-preserved post-implantation roe deer embryos and attempts to assign the embryos to certain development stages. Carnegie stages of human embryos were used as an orientation for staging roe deer embryos. We observed a considerable range of variation of embryonic stages investigated until the end of January. We found post-implantation stages of embryonic development already at the end of December and foetuses at the end of January. Moreover, assigning the embryos to a particular stage of development allows the comparison between pairs of twins and triplets. We showed that twins and triplets were always at the same development level, despite the discrepancy in inter-twin and inter-triplet size.
Asunto(s)
Ciervos/embriología , Diapausa , Desarrollo Embrionario , Animales , Extremidades/embriología , Inmunohistoquímica/veterinaria , Tamaño de la CamadaRESUMEN
E. multilocularis (Em) is the etiologic agent of alveolar echinococcosis (AE), a severe and potentially fatal disease, primarily affecting the liver of and occurring in aberrant intermediate hosts, e.g., humans and non-human primates. Due to increasing numbers of spontaneous cases of AE in the Old World monkey colonies of the German Primate Center, the question arose as to whether vaccination of non-human primates may represent a useful prophylactic approach. In this pilot study, the recombinant antigen Em14-3-3, which has provided a 97 % protection against E. multilocularis challenge infection in rodent models, was used for the first time to immunize rhesus macaques. In order to increase immunogenicity, the antigen was formulated with different adjuvants including Quil A®, aluminum hydroxide (alum), and muramyl dipeptide (MDP). Also, different vaccination regimens were tested. All vaccinated animals developed antigen-specific antibodies. While Quil A® induced a local adverse reaction, alum proved to be the most potent adjuvant in terms of induced antibody levels, longevity as well as tolerability. In conclusion, our pilot study demonstrated that recombinant Em14-3-3 is safe and immunogenic in rhesus monkeys. As a next step, efficacy of the vaccination remains to be explored.
Asunto(s)
Proteínas 14-3-3/inmunología , Anticuerpos Antihelmínticos/sangre , Antígenos Helmínticos/inmunología , Equinococosis Hepática/prevención & control , Echinococcus multilocularis/metabolismo , Adyuvantes Inmunológicos , Animales , Especificidad de Anticuerpos , Equinococosis , Echinococcus/inmunología , Humanos , Macaca mulatta , Masculino , Proyectos Piloto , Proteínas RecombinantesRESUMEN
Simian tuberculosis is one of the most important bacterial diseases of non-human primates. Outbreaks of tuberculosis have been reported in primate colonies almost as long as these animals have been used experimentally or kept in zoological gardens. Significant progress has been made in reducing the incidence of tuberculosis in captive non-human primates, but despite reasonable precautions, outbreaks continue to occur. The most relevant reason is the high incidence of tuberculosis (TB) amongst the human population, in which tuberculosis is regarded as an important re-emerging disease. Furthermore, many non-human primate species originate from countries with a high burden of human TB. Therefore, Mycobacterium tuberculosis remains a significant threat in animals imported from countries with high rates of human infection. We report an outbreak of tuberculosis among a group of rhesus monkeys (Macaca mulatta) living in a closed, long-term colony. The outbreak coincided with reactivation of a TB infection in a co-worker who never had direct access to the animal house or laboratories. Eleven of 26 rhesus monkeys developed classical chronic active tuberculosis with typical caseous granulomata of varying size within different organs. The main organ system involved was the lung, suggesting an aerosol route of infection. Such an outbreak has significant economic consequences due to animal loss, disruption of research and costs related to disease control. Precautionary measures must be improved in order to avoid TB in non-human primate colonies.
Asunto(s)
Brotes de Enfermedades/veterinaria , Enfermedades de los Monos/microbiología , Tuberculosis/veterinaria , Animales , Femenino , Humanos , Macaca mulatta , Masculino , Enfermedades de los Monos/patología , Tuberculosis/patologíaRESUMEN
Conjoined twinning is rare in man and non-human primates. The current report describes a case of ischiopagus tripus conjoined Western Lowland gorilla (Gorilla gorilla) twins. The female twins were joined at the umbilical and pelvic region, involving the liver, xiphoid, umbilicus, body wall and skin. Computed tomography revealed two complete spines. The combined pelvic space was formed by two sacra, each connected with two iliac bones. The twins were only conjoined by a common pubis. Cause of death was attributed to cardiac and circulatory collapse resulting from a large patent foramen ovale (8 mm in diameter) of one twin and neonatal asphyxia.
Asunto(s)
Gorilla gorilla , Gemelos Siameses , Animales , FemeninoRESUMEN
Oncocytic adrenocortical tumours are rare in man and have never been described in non-human primates. An oncocytic adrenocortical carcinoma was identified in an 18-year-old female putty-nosed monkey (Cercopithecus nictitans) with hyperadrenocorticism and invasive aspergillosis. Microscopically, the tumour consisted of large cells with abundant eosinophilic, granular cytoplasm containing numerous mitochondria as identified by electron microscopy. Tumour cells had large nuclei with occasional intranuclear cytoplasmic pseudoinclusions. Immunohistochemically, tumour cells expressed vimentin, synaptophysin and neuron-specific enolase, while they were negative for cytokeratin, chromogranin-A, melan-A and S100.
Asunto(s)
Neoplasias de la Corteza Suprarrenal/veterinaria , Carcinoma Corticosuprarrenal/veterinaria , Neoplasias de la Corteza Suprarrenal/metabolismo , Neoplasias de la Corteza Suprarrenal/patología , Carcinoma Corticosuprarrenal/metabolismo , Carcinoma Corticosuprarrenal/patología , Animales , Biomarcadores de Tumor/análisis , Cercopithecus , Femenino , Inmunohistoquímica , Microscopía Electrónica de Transmisión , Enfermedades de los Monos/metabolismo , Enfermedades de los Monos/patologíaRESUMEN
BACKGROUND: Rhesus monkeys were used as a non-human primate model to study small non-coding RNA after infection with human sporadic and variant Creutzfeldt-Jakob prions. METHODS: Tissue-specific Alu DNA element transcription and editing of transcripts were assessed in neuronal - and blood cells (Buffy Coat). RESULTS: Tissue/cell-specific transcription and editing patterns were obtained. Active Alu DNA elements belonged to several Alu DNA families, they could be located on several chromosomes, and their genomic sites were identified. Deamination by adenosine deaminase acting on RNA and apolipoprotein B editing complex was found. CONCLUSIONS: Different Alu transcription and editing programmes exist and may depend on the infection status.
Asunto(s)
Elementos Alu/genética , ADN/genética , Macaca mulatta , Enfermedades de los Monos/genética , Neuronas/metabolismo , Enfermedades por Prión/veterinaria , Animales , Secuencia de Bases , Cerebelo/citología , Clonación Molecular , Enfermedades de los Monos/patología , Enfermedades por Prión/genética , Enfermedades por Prión/patología , Procesamiento Postranscripcional del ARN , Alineación de SecuenciaRESUMEN
BACKGROUND: An 18-year-old captive female putty-nosed-monkey (Cercopithecus nictitans) with a history of long-term infertility and hyperglucocorticism was euthanized because of perforating thoracic trauma induced by group members and subsequent development of neurological signs. METHODS: Complete necropsy and histopathological examination of formalin-fixed tissue samples was carried out. RESULTS: The monkey showed invasive pulmonary and cerebral infection with Aspergillus fumigatus together with adrenocortical neoplasia and signs of Cushing's syndrome, such as alopecia with atrophic skin changes, evidence for diabetes mellitus and marked immunosuppression. CONCLUSIONS: Spontaneous endocrinopathies are rarely described in non-human primates. Here we report the first case of spontaneous adrenocortical hyperglucocorticism predisposing to systemic aspergillosis in a putty-nosed monkey.
Asunto(s)
Cercopithecus , Aspergilosis Pulmonar Invasiva/veterinaria , Enfermedades de los Monos/patología , Hipersecreción de la Hormona Adrenocorticotrópica Pituitaria (HACT)/veterinaria , Animales , Encéfalo/patología , Femenino , Aspergilosis Pulmonar Invasiva/complicaciones , Aspergilosis Pulmonar Invasiva/patología , Pulmón/patología , Enfermedades de los Monos/inmunología , Hipersecreción de la Hormona Adrenocorticotrópica Pituitaria (HACT)/complicaciones , Hipersecreción de la Hormona Adrenocorticotrópica Pituitaria (HACT)/inmunologíaRESUMEN
An adult male mandrill (Mandrillus sphinx) suffered from chronic ulceration of the facial and gluteal skin and the oral and nasal mucosa. The ulcers were resistant to therapy and led to deterioration in the general condition of the animal. Microscopical examination revealed a severe, chronic, multifocal, granulomatous and eosinophilic dermatitis and panniculitis. There was also stomatitis and rhinitis with numerous intralesional fungal elements. These organisms were identified by immunohistochemistry, transmission electron microscopy, polymerase chain reaction and fungal culture as Candida albicans. Species identification was confirmed by MALDI-TOF mass spectrometry. A specific predisposing immunosuppressive factor for the deep chronic mucocutaneous candidiasis was not identified; however, social stress and/or a primary defect in cell-mediated immunity could not be excluded as possible causes for a predisposing immunodeficiency in the animal.
Asunto(s)
Candida albicans/aislamiento & purificación , Candidiasis Mucocutánea Crónica/veterinaria , Mandrillus , Enfermedades de los Monos/patología , Animales , Antibacterianos/uso terapéutico , Antifúngicos/uso terapéutico , Candida albicans/fisiología , Candidiasis Mucocutánea Crónica/tratamiento farmacológico , Candidiasis Mucocutánea Crónica/inmunología , Candidiasis Mucocutánea Crónica/microbiología , Candidiasis Mucocutánea Crónica/patología , Quimioterapia Combinada/veterinaria , Interacciones Huésped-Patógeno , Huésped Inmunocomprometido , Masculino , Enfermedades de los Monos/tratamiento farmacológico , Enfermedades de los Monos/inmunología , Enfermedades de los Monos/microbiología , Úlceras Bucales/tratamiento farmacológico , Úlceras Bucales/microbiología , Úlceras Bucales/patología , Úlceras Bucales/veterinaria , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/veterinaria , Resultado del TratamientoRESUMEN
Zoonotic orthopoxvirus (OPV) can induce severe disease in man and the virus has potential for use in bioterrorism. New vaccines and therapeutics against OPV infections must be tested in animal models. The aim of this study was to characterize the clinical course and pathology of a new OPV isolate, calpox virus, which is infectious in marmosets. Infection experiments were performed with 28 common marmosets (Callithrix jacchus) exposed to different challenge doses of calpox virus by the intravenous, oropharyngeal and intranasal (IN) routes. The median marmoset IN infectious dose corresponded to 8.3 × 10(2)plaque forming units of calpox virus. Infected animals developed reproducible clinical signs and died within 4-15 days post infection. Characteristic pox-like lesions developed in affected organs, particularly in the skin, mucous membranes, lymph nodes, liver and spleen. Calpox virus disease progression and pathological findings in the common marmoset appear to be consistent with lethal OPV infections in man and in other non-human primate (NHP) models. IN inoculation with low virus doses mimics the natural route of the human variola virus infection. Thus, the marmoset model of calpox virus infection can be considered to be relevant to investigation of the mechanisms of OPV pathogenesis and pathology and for the evaluation of new vaccines and antiviral therapies.
Asunto(s)
Callithrix , Modelos Animales de Enfermedad , Orthopoxvirus , Infecciones por Poxviridae/patología , Animales , Progresión de la Enfermedad , Femenino , Hígado/patología , Hígado/virología , Masculino , Infecciones por Poxviridae/virología , Bazo/patología , Bazo/virologíaRESUMEN
The authors describe genital alterations and detailed histologic findings in baboons naturally infected with Treponema pallidum. The disease causes moderate to severe genital ulcerations in a population of olive baboons (Papio hamadryas anubis) at Lake Manyara National Park in Tanzania. In a field survey in 2007, 63 individuals of all age classes, both sexes, and different grades of infection were chemically immobilized and sampled. Histology and molecular biological tests were used to detect and identify the organism responsible: a strain similar to T pallidum ssp pertenue, the cause of yaws in humans. Although treponemal infections are not a new phenomenon in nonhuman primates, the infection described here appears to be strictly associated with the anogenital region and results in tissue alterations matching those found in human syphilis infections (caused by T pallidum ssp pallidum), despite the causative pathogen's greater genetic similarity to human yaws-causing strains.
Asunto(s)
Enfermedades de los Monos/patología , Papio , Treponema pallidum/aislamiento & purificación , Infecciones por Treponema/veterinaria , Úlcera/veterinaria , Animales , Secuencia de Bases , Femenino , Enfermedades de los Genitales Femeninos/epidemiología , Enfermedades de los Genitales Femeninos/microbiología , Enfermedades de los Genitales Femeninos/patología , Enfermedades de los Genitales Femeninos/veterinaria , Enfermedades de los Genitales Masculinos/epidemiología , Enfermedades de los Genitales Masculinos/microbiología , Enfermedades de los Genitales Masculinos/patología , Enfermedades de los Genitales Masculinos/veterinaria , Masculino , Datos de Secuencia Molecular , Enfermedades de los Monos/epidemiología , Enfermedades de los Monos/microbiología , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Análisis de Secuencia de ADN , Tanzanía/epidemiología , Treponema pallidum/genética , Infecciones por Treponema/epidemiología , Infecciones por Treponema/patología , Úlcera/epidemiología , Úlcera/microbiología , Úlcera/patologíaRESUMEN
BACKGROUND: Upper respiratory tract disease (URTD) is a significant cause of morbidity in captive orangutans (Pongo abelii, Pongo pygmaeus), and the pathogenesis is often unknown. METHODS: The prevalence of respiratory disease in captive European orangutans (201 animals; 20 zoos) and possible predisposing factors were investigated. RESULTS: Bornean orangutans (P. pygmaeus) showed chronic respiratory signs significantly more often (13.8%) than Sumatran (P. abelii; 3.6%), and males (15.8%) were more often afflicted than females (3.9%). Hand-reared animals (21%) developed air sacculitis more often than parent-reared animals (5%). Diseased animals were more often genetically related to animals with respiratory diseases (93%) than to healthy animals (54%). None of the environmental conditions investigated had a significant effect on disease prevalence. CONCLUSION: Results suggest a higher importance of individual factors for the development of URTD than environmental conditions. Bornean, male and hand-reared orangutans and animals related to diseased animals need increased medical surveillance for early detection of respiratory disease.
Asunto(s)
Enfermedades del Simio Antropoideo/epidemiología , Enfermedades del Simio Antropoideo/etiología , Pongo abelii , Pongo pygmaeus , Enfermedades Respiratorias/veterinaria , Animales , Enfermedades del Simio Antropoideo/patología , Europa (Continente) , Femenino , Masculino , Prevalencia , Enfermedades Respiratorias/epidemiología , Enfermedades Respiratorias/etiología , Enfermedades Respiratorias/patología , Estudios Retrospectivos , Factores de Riesgo , Factores Sexuales , Especificidad de la EspecieRESUMEN
Bronchoconstriction is a characteristic symptom of various chronic obstructive respiratory diseases such as chronic obstructive pulmonary disease and asthma. Precision-cut lung slices (PCLS) are a suitable ex vivo model to study physiological mechanisms of bronchoconstriction in different species. In the present study, we established an ex vivo model of bronchoconstriction in nonhuman primates (NHPs). PCLS prepared from common marmosets, cynomolgus macaques, rhesus macaques, and anubis baboons were stimulated with increasing concentrations of representative bronchoconstrictors: methacholine, histamine, serotonin, leukotriene D4 (LTD4), U46619, and endothelin-1. Alterations in the airway caliber were measured and compared with previously published data from rodents, guinea pigs, and humans. Methacholine induced maximal airway constriction, varying between 74 and 88% in all NHP species, whereas serotonin was ineffective. Histamine induced maximal bronchoconstriction of 77 to 90% in rhesus macaques, cynomolgus macaques, and baboons and a lesser constriction of 53% in marmosets. LTD4 was ineffective in marmosets and rhesus macaques but induced a maximum constriction of 44 to 49% in cynomolgus macaques and baboons. U46619 and endothelin-1 caused airway constriction in all NHP species, with maximum constrictions of 65 to 91% and 70 to 81%, respectively. In conclusion, PCLS from NHPs represent a valuable ex vivo model for studying bronchoconstriction. All NHPs respond to mediators relevant to human airway disorders such as methacholine, histamine, U46619, and endothelin-1 and are insensitive to the rodent mast cell product serotonin. Only PCLS from cynomolgus macaques and baboons, however, responded also to leukotrienes, suggesting that among all compared species, these two NHPs resemble the human airway mechanisms best.
Asunto(s)
Broncoconstricción , Pulmón/fisiología , Animales , Broncoconstricción/efectos de los fármacos , Broncoconstrictores/farmacología , Callithrix , Relación Dosis-Respuesta a Droga , Femenino , Cobayas , Humanos , Pulmón/efectos de los fármacos , Macaca fascicularis , Macaca mulatta , Masculino , Ratones , Microscopía por Video , Papio , Ratas , Especificidad de la EspecieRESUMEN
BACKGROUND: A severe upper respiratory tract infection occurred in a breeding group of rhesus monkeys housed together in one of six indoor/outdoor corals of the German Primate Center. The clinical signs of the disease included severe purulent conjunctivitis, rhinitis, pharyngitis, respiratory distress and lethargy. Six of 45 animals died within a few days after developing signs of infection. METHODS AND RESULTS: Histopathologic and microbiologic examinations of the dead animals were consistent with a severe fibrinopurulent bronchopneumonia. Microbiology revealed a Lancefield group C streptococcus identified as Streptococcus equi subsp. zooepidemicus as the causative agent of infection. CONCLUSIONS: The infection was passed on from animal to animal but did not spread to the other five breeding groups nearby. Extensive diagnostic testing failed to reveal the consisting presence of copathogens in individual cases. A visitor with upper respiratory disease was suspected as source of infection.
Asunto(s)
Brotes de Enfermedades/veterinaria , Macaca mulatta , Enfermedades de los Monos/microbiología , Infecciones del Sistema Respiratorio/veterinaria , Infecciones Estreptocócicas/veterinaria , Streptococcus equi/aislamiento & purificación , Animales , Femenino , Pulmón/patología , Masculino , Enfermedades de los Monos/patología , Miocardio/patología , Embarazo , Infecciones del Sistema Respiratorio/microbiología , Infecciones del Sistema Respiratorio/patología , Infecciones Estreptocócicas/microbiología , Infecciones Estreptocócicas/patologíaRESUMEN
Necropsy of a 17-month-old male common marmoset (Callithrix jacchus) with a history of increased abdominal girth resulted in the finding of a unilateral polycystic renal neoplasm. Detailed histopathologic and immunohistochemical investigations revealed different tissue types within the tumor including stromal connective tissue and fusiform mesenchymal cell formations surrounding blastemal cells as well as different developmental stages of organ-specific epithelial cells accompanied by extensive cyst formation. Metastases were not observed. In consideration of the macroscopic, histologic, and immunohistochemical findings, the tumor was classified as a nephroblastoma closely resembling the so-called Wilms' tumor, a malignant embryonic renal tumor frequently observed in humans, especially in young children. In contrast, this tumor entity has rarely been observed in nonhuman primates. This report represents the first documented case of a cystic variant of nephroblastoma in a nonhuman primate.
Asunto(s)
Enfermedades de los Monos/patología , Tumor de Wilms/veterinaria , Animales , Callithrix , Riñón/patología , Masculino , Enfermedades de los Monos/diagnóstico , Tumor de Wilms/diagnóstico , Tumor de Wilms/patologíaRESUMEN
CASE HISTORY: A 4-year-old chimpanzee (Pan troglodytes) had a clinical history of a 2-year progressive central nervous dysfunction including convulsions and severe paralysis. RESULTS: Gross pathology revealed cerebral atrophy, ventricular enlargement and a severe encephalomalacia with extensive calcifications. Histologically, the white matter showed diffuse demyelination as well as vascular and perivascular calcifications which also involved the basal ganglia. Blood vessels with less distinctive calcium deposits exhibited periodic acid Schiff positive hyalinosis. Large areas of necrosis, hemorrhage and intense gliosis were also present. Activation of astrocytes and macrophages was confirmed by immunohistochemical methods. CONCLUSIONS: The etiology of the leucoencepalopathy could not be ascertained by macroscopic, histological and immunohistochemical examinations. Potential differential diagnoses include the rarely occurring Fahr's disease in humans, arteriosclerosis, storage disease and the Aicardi-Goutières syndrome. Based on the results of the postmortal examinations Fahr's disease is regarded as the most likely diagnosis in the present case of the chimpanzee.
Asunto(s)
Enfermedades del Simio Antropoideo/patología , Encefalopatías/veterinaria , Calcinosis/veterinaria , Cerebro/patología , Pan troglodytes/fisiología , Animales , Enfermedades del Simio Antropoideo/diagnóstico , Encefalopatías/diagnóstico , Encefalopatías/patología , Calcinosis/diagnóstico , Calcinosis/patología , FemeninoRESUMEN
BACKGROUND: A 14-year-old female rhesus monkey (Macaca mulatta) of Chinese origin has been suffering from alopecia universalis since childhood. METHODS: Recently, the health status of the animal was recorded comprehensively by detailed clinical examination including hematology and serology supplemented by histological and immunohistochemical investigations of skin biopsies and molecular biological techniques to clarify the causes of the persistent hair loss. RESULTS AND CONCLUSIONS: The hairless gene (hr) nonsense mutation was ruled out by polymerase chain reaction and by sequencing of the corresponding gene. Histological examinations revealed a prominent chronic lymphocytic perifolliculitis and folliculitis affecting anagen stage hair follicles as well as miniaturized hair follicles. Immunohistochemistry using the antibodies CD3, CD20 and CD4 confirmed the diagnosis of a T-cell-mediated autoimmune disease resembling alopecia areata universalis in humans.
Asunto(s)
Alopecia Areata/veterinaria , Enfermedades Autoinmunes/veterinaria , Macaca mulatta , Enfermedades de los Monos/inmunología , Alopecia Areata/genética , Alopecia Areata/inmunología , Animales , Anticuerpos/análisis , Anticuerpos/metabolismo , Antígenos CD/inmunología , Enfermedades Autoinmunes/diagnóstico , Cartilla de ADN/química , Femenino , Folículo Piloso/patología , Enfermedades de los Monos/diagnóstico , Enfermedades de los Monos/genética , Uñas/patología , Reacción en Cadena de la Polimerasa/veterinaria , Piel/inmunología , Piel/patología , Hormonas Tiroideas/sangreRESUMEN
Tularemia is a highly contagious infectious zoonosis, transmissible by inoculation, ingestion, or inhalation of the infectious agent Francisella tularensis. The disease is perpetuated by infected rodents, blood-sucking arthropods, and by contaminated water. Therefore, nonhuman primates housed outdoors may be at risk for exposure. An epizootic of F. tularensis occurred in an indoor/outdoor-housed group of cynomolgus monkeys (Macaca fascicularis) at the German Primate Center. Tularemia was diagnosed in 18 out of 35 animals within a period of 2 years. Six animals died with unspecific clinical symptoms; 12 animals developed seroconversion and were still alive. Pathologic findings were similar in all monkeys that died and resembled the clinical picture of the human disease, including an ulceroglandular syndrome with local lymphadenopathy, gingivostomatitis, and systemic spread, with manifestations such as subacute necrotizing hepatitis, granulomatous splenitis, and pneumonia. Tularemia was diagnosed by culture, real-time polymerase chain reaction, and ELISA techniques. This is the largest outbreak in nonhuman primates and the first report of tularemia in cynomolgus monkeys. An overview of the recent literature about tularemia in nonhuman primates is given.
Asunto(s)
Macaca fascicularis , Enfermedades de los Monos/diagnóstico , Tularemia/veterinaria , Animales , Femenino , Gingivitis/patología , Gingivitis/veterinaria , Vivienda para Animales , Riñón/patología , Hígado/patología , Pulmón/patología , Masculino , Enfermedades de los Monos/patología , Bazo/patología , Lengua/patología , Tularemia/diagnóstico , Tularemia/patologíaRESUMEN
Concerning the public discussion on animal experiments using primates, various research fields are demonstrated where non-human primates are necessary for certain scientific reasons at this time. Non-human Primates are used in Germany mainly in regulatory toxicology and pharmaceutical safety studies.A small amount is disposed in different fields of biological or biomedical basic research. This includes in particular neurosciences and infection research. 2006 New and Old World monkeys were needed in Germany in 2005. No chimpanzees are used anymore as laboratory animals in Germany since many years. Several examples are presented to demonstrate that certain research fields need non-human primates as laboratory animals in the foreseeable future.
Asunto(s)
Ciencia de los Animales de Laboratorio/métodos , Primates , Investigación/normas , Animales , Bioética , Alemania , Ciencia de los Animales de Laboratorio/normas , Farmacología/métodos , Toxicología/métodosRESUMEN
An epizootic infection was observed in a colony of 80 New World monkeys consisting of various species including a group of marmosets and Saguinus species. During the summer and autumn of 2002, 30 animals died of unknown diseases. Six animals were sent to the German Primate Center for investigation of the cause of death. A complete pathologic and histologic investigation was carried out. The animals exhibited erosive-ulcerative lesions of the oral mucous membranes. Advanced stages of the disease were characterised by hemorrhagic lesions on the skin distributed randomly over the body, but principally on the face, scrotal region, soles, and palms. Electron microscopy revealed virus particles with orthopox-like morphology within intracytoplasmic inclusions in epithelial cells. The DNA samples from various tissues were analyzed by use of a set of orthopox virus-specific, real-time polymerase chain reaction assays. Amplification products were sequenced to define the virus more precisely. Sequencing confirmed the presence of an orthopox virus. Sequence data indicated that all six animals were infected with the same virus. Propagation of the virus on Vero cells resulted in a rapidly progressive cytopathogenic effect. Preliminary phylogenetic analyses of two genes revealed closest homology to cowpox viruses. The origin of this poxvirus outbreak remains unexplained, and the strain and genus of the virus need to be determined in detail.
Asunto(s)
Brotes de Enfermedades/veterinaria , Enfermedades de los Monos/epidemiología , Enfermedades de los Monos/virología , Orthopoxvirus/aislamiento & purificación , Platirrinos/virología , Animales , Femenino , Masculino , Enfermedades de los Monos/patología , Piel/patologíaRESUMEN
Tubuloreticular structures (TRS) are considered to be a specific ultrastructural marker for AIDS in various organs. Experimental SIV infection in rhesus macaques is the most appropriate animal model of HIV infection. In 8 rhesus monkeys, experimentally infected with SIVmac251/MPBC, rectum biopsies were taken prior to and post infection (day 3; 1, 2, 4, 12 weeks p.i.) and were investigated by transmissionelectron microscopy to determine incidence and extent of tubuloreticular structures as well as affected cells. From the first week p.i. on TRS were found in all experimental animals as tubuli with a diameter of 20-30 nm. The tubuli were arranged in regular paracristalline formations and formed intracytoplasmatic heterogenous, polymorph accumulations, which were localized close to the endoplasmatic reticulum. In the rectal lamina propria macrophages, endothelial cells, plasma cells, lymphocytes, fibroblasts, and neutrophilic granulocytes were the affected cell types. In 5 control biopsies TRS were detected, too, but, in contrast to SIV-infected animals, they appeared only singular and very small. The results indicate that TRS are a characteristic morphologic criteria of intestinal SIV infection. They appear in very early stages of the infection. In the rectum, they can be detected as bigger, conspicuous, and abundant formations in several cells and have a restricted diagnostic and prognostic validity.