RESUMEN
Viscumin (mistletoe lectin I, MLI) in concentrations of 10-11-10-7 M causes endoplasmic reticulum stress and triggers unfolded protein response, a modulator of antitumor immunity, in target cells.
Asunto(s)
Estrés del Retículo Endoplásmico , Proteínas Inactivadoras de Ribosomas Tipo 2/farmacocinética , Toxinas Biológicas/farmacocinética , Animales , Células CACO-2 , Línea Celular Tumoral , Estrés del Retículo Endoplásmico/efectos de los fármacos , Humanos , Inyecciones Subcutáneas , Masculino , Ratones , Lectinas de Plantas/administración & dosificación , Lectinas de Plantas/farmacocinética , Lectinas de Plantas/farmacología , Proteínas Inactivadoras de Ribosomas Tipo 2/administración & dosificación , Proteínas Inactivadoras de Ribosomas Tipo 2/farmacología , Toxinas Biológicas/administración & dosificación , Toxinas Biológicas/farmacología , Respuesta de Proteína Desplegada/efectos de los fármacosRESUMEN
Microarray analysis of cultured endothelial cells was performed 24 h after simulated microgravity. A significant change in the expression of 177 genes that can be classified into several functional clusters was detected. Among the genes with overexpression, clusters of cell response to external stimuli and regulation of cell motility and proliferation can be reliably distinguished. Among down-regulated genes, clusters of transcription factors with the "zinc fingers" domain and factors involved in the regulation of morphogenesis and angiogenesis were distinguished. The overlapping of signaling pathways involved in mechanotransduction and inflammatory changes is discussed.
Asunto(s)
Células Endoteliales de la Vena Umbilical Humana/metabolismo , Transcriptoma , Ingravidez , Movimiento Celular/genética , Proliferación Celular/genética , Células Endoteliales de la Vena Umbilical Humana/fisiología , Humanos , Factores de Transcripción/genética , Factores de Transcripción/metabolismoRESUMEN
In this study, we performed transcriptome profiling of oligodendrocyte culture of mice treated with the remyelinating therapeutic agent benztropine in the presence and absence of interferon gamma (IFNγ). The results of this work are important for understanding the expression profile of oligodendrocytes under conditions of systemic inflammation in the central nervous system in multiple sclerosis as well as the mechanisms of cellular response to benztropine in light of its possible use for the treatment of multiple sclerosis.
Asunto(s)
Oligodendroglía/inmunología , ARN Mensajero/inmunología , Transcriptoma/inmunología , Animales , Antiinflamatorios/farmacología , Benzotropina/farmacología , Células Cultivadas , Perfilación de la Expresión Génica , Factores Inmunológicos/farmacología , Interferón gamma/farmacología , Ratones Endogámicos C3H , Oligodendroglía/efectos de los fármacos , Complejo de la Endopetidasa Proteasomal/efectos de los fármacos , Complejo de la Endopetidasa Proteasomal/inmunología , Transcriptoma/efectos de los fármacosRESUMEN
We compared the results of real-time PCR analysis of gene expression in paired specimens breast cancer tissue fixed in RNAlater (Qiagen) stabilization reagent (FF samples) and in formalin (FFPE samples). A clear-cut linear relationship (correlation coefficient 0.76 ± 0.07) was detected between the gene expression logarithm values in FF and FFPE samples. This fact suggests that collections of paraffin blocks with formalin-fixed tissue specimens from patients with a many-year disease history can be effectively used in modern studies.
Asunto(s)
Neoplasias de la Mama/metabolismo , Carcinoma Ductal de Mama/metabolismo , Perfilación de la Expresión Génica/métodos , Fijación del Tejido/métodos , Neoplasias de la Mama/genética , Neoplasias de la Mama/patología , Carcinoma Ductal de Mama/genética , Carcinoma Ductal de Mama/patología , Femenino , Fijadores/química , Formaldehído/química , Expresión Génica , Humanos , Persona de Mediana Edad , Estabilidad del ARN , Juego de Reactivos para DiagnósticoRESUMEN
Inflammatory breast cancer is characterized by high malignancy, early and rapid lymphogenic and hematogenic metastasizing, and high mortality. The diagnosis of this form of cancer is based fully on the clinical criteria. Whole transcriptome analysis of tumor tissue specimens from patients with inflammatory breast cancer detected 137 differentially expressed mRNA (17 genes with low expression and 120 with high expression). Genes involved in the organization of inflammatory process, chemotaxis, and transcription regulation were active in the process of pathogenesis of inflammatory breast cancer.