The Politicization of the COVID-19 Pandemic.

Political actors and institutions are largely responsible for effectively implementing the latest scientific and medical information in the form of public health measures. However, when politicians' judgments and decision-making are not founded on scientific facts or when scientific findings are misrepresented to further political goals, global crises such as pandemics may be even more galvanized. Like other scientific topics that entered public debate before 2020 (e.g., the debate on climate change), the COVID-19 pandemic has been heavily politicized worldwide. Consequently, COVID-19-related outcomes were strongly affected by politicization-a process of making a non-political issue political, i.e., debating it in the public sphere as an issue of public contestation. The present chapter presents a condensed overview and synthesis of the literature on the politicization of the COVID-19 pandemic in high- and low-income countries. In addition, we discuss several mechanisms explaining why, to some extent universally, conservatives (the right-wing oriented public) were less likely to follow public health recommendations, were more COVID-19 vaccine-hesitant, and had increased infection rates, poor health outcomes, and increased mortality compared to left-wing oriented public. The mechanisms explaining the links include the media, trust, cognitions, and values. We conclude the chapter with lessons learned during the COVID-19 pandemic and future research directions on the pandemics' politicization.

Predictors of Meat Reduction: The Case of Slovenia.

Health, environmental, and animal advocacy organisations emphasise reducing or eliminating high meat consumption due to its adverse effects on health, sustainability, climate change, and animal welfare. Increasingly, people are deciding to reduce their meat consumption frequency. Our study aimed to examine predictors of meat reduction among Slovenian consumers, focusing on gender, age, partner and children status, size of residential settlement, socioeconomic status, and political orientation. We conducted a survey using non-probability sampling. We examined demographic, socioeconomic, and political predictors of individuals' self-assessed intent to reduce meat consumption in the month following the survey. Additionally, we analysed respondents' meat reduction during the three years prior. A correlation analysis revealed that higher age and education levels were significantly positively correlated with meat reduction patterns. An ordinal logistic regression analysis indicated that age was the only significant predictor of meat reduction intentions and past behaviour. Our findings suggest that middle-aged and elderly individuals are more likely than younger adults to report meat reduction behaviours. Future public health interventions should tailor approaches to different age groups, and, in particular, target younger individuals. Educational campaigns should highlight the health and environmental benefits of reducing meat and animal product consumption, particularly in primary and secondary schools.

Exploring the Inherent Heterogeneity of Vaccine Hesitancy: A Study of a Childhood-Vaccine-Hesitant Population.

Vaccine hesitancy and its determinants have been previously widely researched. Vaccine hesitancy has been defined as a continuum of attitudes, ranging from accepting vaccines with doubts to rejecting them. The present study aims to explore the heterogeneity of a childhood-vaccine-hesitant group by using a person-oriented approach-latent profile analysis. A non-representative cross-sectional sample of vaccine-hesitant Slovenians (N = 421, Mage = 35.21, 82.9% women) was used to identify differences based on their reliance on personal research ("self" researching instead of relying on science), overconfidence in knowledge, endorsement of conspiracy theories, complementary and alternative medicine, and trust in the healthcare system. The analysis revealed three profiles of vaccine-hesitant individuals. The most hesitant profile-vaccine rejecting-expressed the greatest reliance on personal research, expressed the highest endorsement of conspiracy theories and complementary and alternative medicine, showed moderate overconfidence in their knowledge, and expressed the highest levels of distrust in the healthcare system. We further found differences in sociodemographic structure and that the identified profiles differed in their attitudes regarding MMR, HPV, and Seasonal Influenza vaccinations. The present study demonstrates the heterogeneity of the vaccine-hesitant community and offers insights into some of the traits, which are crucial for designing pro-vaccine campaigns.

Okadaic acid as a major problem for the seafood safety (Mytilus galloprovincialis) and the dynamics of toxic phytoplankton in the Slovenian coastal sea (Gulf of Trieste, Adriatic Sea).

This article presents the first results on shellfish toxicity in the Slovenian sea (Gulf of Trieste, Adriatic Sea) since the analytical methods for the detection of biotoxins (PSP, ASP, DSP and other lipophilic toxins) in bivalve molluscs were included in the national monitoring program in 2013. In addition to toxins, the composition and abundance of toxic phytoplankton and general environmental characteristics of the seawater (surface temperature and salinity) were also monitored. During the 2014-2019 study period, only lipophilic toxins were detected (78 positive tests out of 446 runs), of which okadaic acid (OA) predominated in 97 % of cases, while dinophysistoxin-2 and yessotoxins only gave a positive result in one sampling event each. The number of samples that did not comply with the EC Regulation for the OA group was 17 or 3.8 % of all tests performed, all of which took place from September to November, while a few positive OA tests were also recorded in December, April, and May. This toxicity pattern was consistent with the occurrence pattern of the five most common DSP-producing dinoflagellates, which was supported by the development of warm and thermohaline stratified waters: Dinophysis caudata, D. fortii, D. sacculus, D. tripos and Phalacroma rotundatum. The strong correlation (r = 0.611, p < 0.001) between D. fortii, reaching abundances of up to 950 cells L-1, and OA suggests that D. fortii is the main cause of OA production in Slovenian waters. Strong interannual variations in OA and phytoplankton dynamics, exacerbated by the effects of anthropogenic impacts in this coastal ecosystem, reduce the predictability of toxicity events and require continuous and efficient monitoring. Our results also show that the introduction of the LC-MS/MS method for lipophilic toxins has improved the management of aquaculture activities, which was not as accurate based on mouse bioassays.

Multilevel analysis of COVID-19 vaccination intention: the moderating role of economic and cultural country characteristics.

BACKGROUND: Predictors of COVID-19 (coronavirus) vaccination have been extensively researched; however, the contextual factors contributing to understanding vaccination intention remain largely unexplored. The present study aimed to investigate the moderating role of economic development (Gross domestic product - GDP per capita), economic inequality (Gini index), the perceived corruption index and Hofstede's measurements of cultural values-index of individualism/collectivism and power distance index-in the relationship between determinants of satisfaction with the healthcare system, trust in political institutions, conspiracy beliefs and COVID-19 vaccination intention. METHODS: A multilevel modelling approach was employed on a sample of approximately 51 000 individuals nested within 26 countries. Data were drawn from the European Social Survey Round 10. The model examined the effect of individual- and country-level predictors and their interaction on vaccination intention. RESULTS: Satisfaction with the healthcare system had a stronger positive effect on intention to get vaccinated in countries with lower perceived corruption and more individualistic countries. Trust in political institutions had a stronger positive effect on vaccination intention in countries with higher economic development and lower perceived corruption, while a negative effect of conspiracy beliefs on vaccination intention was stronger in countries with lower economic development, higher perceived corruption and a more collectivistic cultural orientation. CONCLUSION: Our findings highlight the importance of considering individual and contextual factors when addressing vaccination intention.

Farm Animal Welfare during Transport and at the Slaughterhouse: Perceptions of Slaughterhouse Employees, Livestock Drivers, and Veterinarians.

Animal welfare is a multidimensional concept that includes several physical and psychological parameters of the animal. The aim of this study was to assess animal welfare during transportation and in Slovenian beef, pork, and poultry slaughterhouses. A questionnaire was used for this study. Several parameters of animal welfare were rated on a 5-point scale, such as health status, animal behavior, lairage or transport vehicle conditions, and driver regulation compliance. The scale was also used for the second part of the study. This consisted of two studies: (1) self-assessment by slaughterhouse employees and livestock transport drivers and (2) animal welfare observational assessment performed by two veterinarians. The results were compared with each other. Ten large slaughterhouses and nine livestock drivers took part in the survey. The results showed that slaughterhouse employees knew more about animal welfare than livestock truck drivers, but both groups were not sufficiently familiar with animal welfare laws and regulations. This study found that the experts' assessments were generally lower than the self-assessments of employees and livestock drivers. Based on the research findings, it can be concluded that there is a need to improve the awareness and knowledge of slaughterhouse employees and livestock drivers regarding animal handling and animal welfare regulations.

Ideological differences in COVID-19 vaccine intention: the effects of trust in the healthcare system, in complementary and alternative medicine, and perceived threat from the disease.

Introduction: Politically left-leaning individuals are more likely to get vaccinated against COVID-19, although little is known about the mechanisms underlying the ideological differences in vaccination intentions. Understanding the extent to which trust in the healthcare system, in complementary and alternative medicine, and the perceived threat from the disease contribute to these disparities is crucial, as it could inform targeted interventions to address vaccine hesitancy across the political spectrum. Methods: The present cross-sectional study conducted among adults living in Slovenia (n = 858) examined the mediating role of trust in the healthcare system, trust in complementary and alternative medicine (CAM), and the perceived threat from the virus on COVID-19 vaccination intention. Results: We found that leftist ideology and trust in the healthcare system positively predicted vaccination intention, whereas CAM negatively predicted this intention. In addition, left-leaning individuals expressed greater trust in the healthcare system and lower trust in CAM, resulting in higher levels of COVID-19 vaccination intention. The serial mediation model confirmed that trust in CAM was a negative predictor, while trust in the healthcare system positively predicted perceived threat. Discussion: When dealing with vaccine hesitancy among right-oriented individuals, strategies should focus on enhancing trust in the healthcare system and critically evaluating the reliance on CAM.

Food Safety Knowledge among Professional Food Handlers in Slovenia: The Results of a Nation-Wide Survey.

The authors present and discuss the results of a nation-wide survey on food safety knowledge among professional food handlers in Slovenia. The data were collected via a telephone survey using a well-established questionnaire adapted to the Slovenian context. Altogether, 601 respondents from hotels, restaurants, catering, and confectionery units completed the questionnaire. To assess food safety knowledge among food handlers in both general and specific domains, three indexes (a General Knowledge Index, a Personal Knowledge Index, and a Temperature Knowledge Index) were created. Among them, the Temperature Knowledge Index revealed the largest gaps in knowledge. An insufficient transfer of food safety knowledge from managers and chefs to assistant chefs and kitchen assistants in establishments where more persons handle food was evident, while a course titled "Hygiene Minimum" of standardised training from the past still significantly contributes to food safety knowledge. The results suggest a need for improvement in the current system of food safety training courses for professional food handlers in Slovenia. The human factor in the food supply chain still has a significant role in ensuring food safety culture, and therefore must become a more important part of the food safety management system.

The Impact of Cultural Capital on Vaccine Attitudes among the Slovenian Public.

Education and highbrow cultural participation-two dimensions of cultural capital-have previously been identified as determinants of vaccine attitudes, though the links have been mainly inconsistent across studies. The present quantitative study aimed to examine the effects of two dimensions of cultural capital (institutionalized and embodied cultural capital) on the pro-vaccine attitudes of the Slovenian public. A cross-sectional quantitative study was performed in November 2019, a few months prior to the COVID-19 pandemic. The non-probability sample survey was collected by inviting respondents over the age of 18 to participate using the snowball technique via e-mail, digital social networks (Facebook, Twitter and Instagram) and University of Maribor social network profiles. The sample was obtained through an online survey tool 1ka.si (N = 661; Mage = 34.9 years). The impact of education and highbrow cultural participation on vaccine attitudes was examined, controlling for sociodemographic variables (gender, age and size of residential settlement) and economic variables (income and family economic status) in multivariate analyses. Bivariate analyses indicated that pro-vaccine attitudes were significantly more likely to be expressed by men, younger respondents, those with lower incomes, higher perceived family economic status, living in urban areas and by those who are more frequently engaged in highbrow cultural activities, while education had no impact on vaccine attitudes. The results did not substantially change when multiple regression models were employed. Our study indicated that cultural capital has an inconsistent impact on vaccine attitudes; while education has no impact, highbrow cultural participation increases pro-vaccine attitudes. The results suggest a multi-type approach is needed to address vaccine scepticism among the Slovenian public.

Distrustful, Dissatisfied, and Conspiratorial: A Latent Profile Analysis of COVID-19 Vaccination Rejection.

Trust in science and scientists, satisfaction with the national government, and endorsement of conspiracy theories are important factors in the decision to be vaccinated. In this study, we investigated whether there are different profiles of individuals depending on the above factors and whether they differ in their sociodemographic composition. We used data from Round 10 of the European Social Survey for Slovenia, employing a nationally representative sample of 1252 participants. Based on latent profile analysis, three distinct profiles emerged: Profile 1 expressed moderate trust in science, satisfaction with government, and high endorsement of conspiracies; Profile 2 expressed low trust and satisfaction and moderate endorsement of conspiracies; Profile 3 expressed high trust and satisfaction and low beliefs in conspiracy theories. In addition, Profile 3 expressed the strongest support for vaccination and Profile 2 the lowest. Our results suggest that distrust, dissatisfaction, and the presence of conspiracy theories are the "perfect storm" for vaccination rejection. In contrast, despite conspiracy theories, a certain level of trust and satisfaction may reduce vaccination rejection.

Cannabis Use and Parenting Practices among Young People: The Impact of Parenting Styles, Parental Cannabis-Specific Rules, and Parental Cannabis Use.

Cannabis is the most commonly used illicit drug. Its use typically starts in adolescence, and parents play a key role in young people's cannabis use. Our study aimed to examine and compare the effects of parenting styles, parental cannabis-specific rules, and parental cannabis use on young people's cannabis use. The research sample consisted of 839 students from various secondary education programs in Slovenia, aged 14 to 21. Associations between the young people's lifetime cannabis use and their experience of parenting practices were assessed using logistic regression, with demographic, socioeconomic, educational, health, and risk behaviors controlled in a multivariate model. Maternal authoritative parenting (in comparison with permissive parenting), strict maternal, cannabis-specific rules, and parental cannabis non-use statistically significantly reduced the likelihood of young people's cannabis use. Its strongest predictor was parental cannabis use, followed by the mother's specific cannabis-use rules and maternal parenting style. The findings of our study can contribute to the development of public health policies to more effectively prevent cannabis use among adolescents and emerging adults, including by designing prevention programs aimed at strengthening parents' general and cannabis-specific practices and competences.

Modeling Paratuberculosis Transmission in a Small Dairy Herd Typical of Slovenia Suggests That Different Models Should Be Used to Study Disease Spread in Herds of Different Sizes.

This study aimed to investigate the possible dynamics of paratuberculosis or Johne's disease in a typical Slovenian dairy herd of about 17 cows. Paratuberculosis is a worldwide endemic disease of cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is associated with significant economic losses. We developed a stochastic compartmental model with two pathways of disease progression, infections of adult cows and infections of young animals through horizontal and vertical transmission, and transmission through animal movements. The average proportions of subclinically and clinically infected cows were 4% and 0.47%, respectively. The prevalence within the herd, which included latently infected animals, averaged 7.13% and ranged from 0% to 70.59%. Under the given circumstances, the results showed a relatively high rate of spontaneous elimination (0.22 per herd per year) of the disease and a high rate of reinfection (0.18 per herd per year) facilitated by active animal trade. To our knowledge, this stochastic compartmental model is the first to be developed specifically to represent a small dairy herd and could apply to other countries with a similar structure of dairy farms. The results suggest that different models should be used to study MAP spread in herds of various sizes.

Quantitative Risk Assessment of Exposure to Mycobacterium avium subsp. paratuberculosis (MAP) via Different Types of Milk for the Slovenian Consumer.

This study aimed to assess the risk of exposure to Mycobacterium avium subsp. paratuberculosis (MAP) via milk for the Slovenian consumer. MAP is suspected to be associated with several diseases in humans, therefore the risk of exposure should be better understood. The primary source of MAP for humans is thought to be cattle, in which MAP causes paratuberculosis or Johne's disease. We developed a stochastic quantitative risk assessment model using Monte Carlo simulations. Considering the assumptions and uncertainties, we estimated the overall risk of exposure to MAP via milk to be low. For people consuming raw milk from MAP positive farms, the risk was high. On-farm pasteurisation reduced the risk considerably, but not completely. The risk of exposure via pasteurised retail milk was most likely insignificant. However, with a higher paratuberculosis prevalence the risk would also increase. Given the popularity of raw milk vending machines and homemade dairy products, this risk should not be ignored. To reduce the risk, consumers should heat raw milk before consumption. To prevent a potential public health scare and safeguard farmers' livelihoods, a reduction in paratuberculosis prevalence should be sought. Our results show that culling clinically infected cows was insufficient to reduce milk contamination with MAP.

A molecular survey, whole genome sequencing and phylogenetic analysis of astroviruses from roe deer.

BACKGROUND: Although astroviruses (AstV) have been detected in a variety of host species, there are only limited records of their occurrence in deer. One of the most important game species in Europe, due to its meat and antlers, is roe deer. Infected game animals can pose a threat to the health of other animals and of humans, so more attention needs to be focused on understanding the diversity of viruses in wildlife. The complete genome and organization of the roe deer AstV genome have not so far been described. RESULTS: In our study, 111 game animals were screened for the presence of AstV. While no AstVs were detected in red deer, wild boar, chamois and mouflon, AstV RNA was present in three samples of roe deer. They were further subjected to whole genome sequencing with next generation sequencing. In this study, two AstV genomes were assembled; one in sample D5-14 and one in sample D12-14, while, in sample D45-14, no AstV sequences were identified. The complete coding sequences of the AstV SLO/D5-14 strain genome and of the almost complete genome of the AstV SLO/D12-14 strain were determined. They showed a typical Mamastrovirus organization. Phylogenetic analyses and amino acid pairwise distance analysis revealed that Slovenian roe deer AstV strains are closely related to each other and, also, related to other deer, bovine, water buffalo, yak, Sichuan takin, dromedary, porcine and porcupine AstV strains - thus forming a highly supported group of currently unassigned sequences. CONCLUSIONS: Our findings suggest the existence of a new Mamastrovirus genogroup might be constituted while this aforementioned group is distantly related to Mamastrovirus genogroups I and II. In this study, additional data supporting a novel taxonomic classification are presented.

Screening and Molecular Characterization of Hepatitis E Virus in Slaughter Pigs in Serbia.

Hepatitis E virus (HEV) is a zoonotic virus that can cause acute hepatitis in humans. Besides the fecal-oral route, transmission can occur by consumption of undercooked pig liver. Genotype 3 is the most frequent genotype found in Europe. Studies on HEV in slaughter-age pigs have not been conducted in Serbia so far. Pork meat production and consumption in Serbia is on average, higher than in the rest of Europe. With the aim to identify the circulating HEV genotypes, pig livers and swab samples from three pig slaughterhouses located in three different sub-regions of Serbia were collected. A nested RT-PCR was used to amplify the hypervariable HEV ORF-1 region (334 bp). The amplicons yielded in this study were sequenced, and a molecular phylogeny analysis based on the maximum likelihood method, including HEV sequences reported in several other countries, was performed. The average prevalence of HEV genotype 3 in 3-month-old pigs was 34%. Phylogenetic analysis revealed the majority of HEV amplification fragments from Serbia were grouped in four clades within sub-genotype 3a and were also genetically related to German, Italian, Slovenian, and American HEV sequences. Sub-genotypes 3b and 3j were also found in a single pig each. This study provides the first analysis of the genetic diversity and circulation dynamics of HEV in pigs at slaughterhouses in Serbia.

Trace Element Concentrations in Mussels ( Mytilus galloprovincialis) from the Gulf of Trieste, Slovenia.

The concentrations of the trace elements zinc (Zn), arsenic (As), manganese (Mn), copper (Cu), chromium (Cr), nickel (Ni), cobalt (Co), cadmium (Cd), lead (Pb), mercury (Hg), and iron (Fe) were examined in Mediterranean blue mussels ( Mytilus galloprovincialis) from the Northern Adriatic Sea to determine whether there were site differences and seasonal variations in metal concentrations and to evaluate the potential health risks of consumption of these mussels to humans. Mussels were collected between January and October 2015 from three Slovenian shellfish harvesting areas and from a lighthouse near Debeli Rtic. The concentrations of elements were determined using inductively coupled plasma mass spectrometry. The amount of each element decreased in the following order: Fe > Zn > (As, Mn) > (Cu, Cr) > (Ni, Co) > (Cd, Pb) > Hg. Statistical analysis revealed that time, that is, the month of sampling, significantly affected the concentrations of elements in mussels ( P < 0.05). The concentrations of Cr, Mn, Fe, Ni, Hg, and Pb were highest between January and March and lowest between May and September. The concentrations of other elements in mussels were not higher or lower for several consecutive months, but occasionally (some months) lower or higher content was detected. The sampling site had a statistically significant effect only on the content of Cd, Pb, and Hg in mussels ( P < 0.05). The lowest level of these elements was found in mussels from Seca, and the highest levels of Hg and Pb were found in mussels from Debeli Rtic. Debeli Rtic is, among all sampling points, closest to the outflow of the Soca River that brings heavy metals into the Northern Adriatic Sea from a closed Hg mine. Because the concentrations of Pb, Cd, and Hg in all analyzed samples were below the maximum level permitted by Commission Regulation No 1881/2006, mussels were suitable for human consumption.

Implications of Cattle Trade for the Spread and Control of Infectious Diseases in Slovenia.

The objectives of this study were to gain insight into the structure of the cattle trade network in Slovenia and to evaluate the potential for infectious disease spread through movements. The study considered cattle movements between different types of premises that occurred between August 1, 2011 and July 31, 2016 with the exclusion of the movements to the end nodes (e.g., slaughterhouses). In the first part, we performed a static network analysis on monthly and yearly snapshots of the network. These time scales reflect our interest in slowly spreading pathogens; namely Mycobacterium avium subsp. paratuberculosis (MAP), which causes paratuberculosis, a worldwide economically important disease. The results showed consistency in the network measures over time; nevertheless, it was evident that year to year contacts between premises were changing. The importance of individual premises for the network connectedness was highly heterogeneous and the most influential premises in the network were collection centers, mountain pastures, and pastures. Compared to random node removal, targeted removal informed by ranking based on local network measures from previous years was substantially more effective in network disassociation. Inclusion of the latest movement data improved the results. In the second part, we simulated disease spread using a Susceptible-Infectious (SI) model on the temporal network. The SI model was based on the empirically estimated true prevalence of paratuberculosis in Slovenia and four scenarios for probabilities of transmission. Different probabilities were realized by the generation of new networks with the corresponding proportion of contacts which were randomly selected from the original network. These diluted networks served as substrates for simulation of MAP spread. The probability of transmission had a significant influence on the velocity of disease spread through the network. The peaks in daily incidence rates of infected herds were observed at the end of the grazing period. Our results suggest that network analysis may provide support in the optimization of paratuberculosis surveillance and intervention in Slovenia. The approach of simulating disease spread on a diluted network may also be used to model other transmission pathways between herds.

Development and Validation of a New Protocol for Detecting and Recovering Clostridium difficile from Meat Samples.

There is no recommended protocol for detecting and isolating Clostridium difficile present in food samples. Here, we have evaluated the recovery of C. difficile in meat samples after incubating them in various enrichment broths. The media were as follows: cycloserine-cefoxitin fructose broth supplemented with taurocholic acid, d-cycloserine, cefoxitin, and lysozyme; cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme; and cycloserine-cefoxitin fructose broth supplemented with taurocholic acid, C. difficile moxalactam norfloxacin selective supplement, and lysozyme. Samples were inoculated with various strains and quantities of C. difficile and then enriched in the different broths for 1, 4, and 7 days. C. difficile was isolated on agar plates and detected with quantitative real-time PCR (qPCR). The procedure using enrichment in cycloserine-cefoxitin fructose broth supplemented with taurocholic acid, d-cycloserine, cefoxitin, and lysozyme and incubation for 4 days for qPCR detection and 7 days for isolation (plating on C. difficile agar base with added C. difficile selective supplement and 7% [v/v] defibrinated horse blood after alcoholic shock and centrifugation) was validated. Samples of different kinds of meat and meat preparation were contaminated and used for validation of the chosen protocol. The sensitivity of detection with qPCR was 100%, and the sensitivity of the isolation method was 96%.

A comprehensive study of hepatitis E virus infection in pigs entering a slaughterhouse in Slovenia.

Hepatitis E is a zoonotic viral disease of pigs with increasing public health concern in industrialized countries. Presented broad study of hepatitis E virus (HEV) presence in pigs in Slovenia is the first attempt to overview the HEV situation in pigs entering a slaughterhouse and, further, to analyse the possibility of HEV entering into the food supply chain. 2433 samples from 811 clinically healthy pigs were collected at four slaughterhouses in Slovenia. Sampling covered three different age groups of pigs and three different types of samples (faeces, bile and liver) important for tracing HEV in a pig population. In addition, 63 swab samples were collected systematically from three different sites on the slaughter line, as well as 22 samples of minced meat and 30 bratwurst samples. All the samples were screened for the presence of HEV nucleic acids by specific real-time RT-PCR assay. In the group of three month old pigs 13.7% of faeces, 13.0% of bile and 2.1% of liver samples were HEV positive. In the group of six months old pigs only 0.25% of liver and 0.25% of bile samples were positive. In the category of sows, no positive samples were found. Two out of 63 swab samples collected on the slaughter line were HEV positive. All tested samples of minced meat and bratwurst were negative. The phylogenetic analysis of 50 HEV positive samples, with comparison of 366 nucleotides in ORF1 region, revealed high diversity of identified strains of HEV in pigs, belonging into subtypes 3a, 3b, 3c and 3e.

Whole genome sequence and a phylogenetic analysis of the G8P[14] group A rotavirus strain from roe deer.

BACKGROUND: Group A rotaviruses (RVA) are associated with acute gastroenteritis in children and in young domestic and wild animals. A RVA strain was detected from a roe deer for the first time during a survey of game animals in Slovenia in 2014. A further RVA strain (SLO/D110-15) was detected from a roe deer during 2015. The aim of this study was to provide a full genetic profile of the detected RVA strain from roe deer and to obtain additional information about zoonotic transmitted strains and potential reassortments between human rotavirus strains and zoonotic transmitted rotavirus strains. The next generation sequencing (NGS) analysis on Ion Torrent was performed and the whole genome sequence has been determined together with a phylogenetic analysis. RESULTS: The whole genome sequence of SLO/D110-15 was obtained by NGS analyses on an IonTorrent platform. According to the genetic profile, the strain SLO/D110-15 clusters with the DS-1-like group and expresses the G8-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3 genome constellation. Phylogenetic analysis shows that this roe deer G8P[14] strain is most closely related to RVA strains found in sheep, cattle and humans. A human RVA strain with the same genotype profile was detected in 2009 in Slovenia. CONCLUSIONS: The G8P[14] genotype has been found, for the first time, in deer, a newly described host from the order Artiodactyla for this RVA genotype. The finding of a rotavirus with the same genome segment constellation in humans indicates the possible zoonotic potential of this virus strain.