RESUMEN
Preen oil, the secretion from the uropygial gland of birds, may have a specific function in incubation. Consistent with this, during incubation, the chemical composition of preen oil is more likely to differ between sexes in species where only one sex incubates than in species where both sexes incubate. In this study, we tested the generality of this apparent difference, by investigating sex differences in the preen oil composition of a shorebird species, the Kentish plover (Anarhynchus, formerly Charadrius, alexandrinus). As both sexes incubate in this species, we predicted the absence of sex differences in preen oil composition during incubation. In the field, we sampled preen oil from nine females and 11 males during incubation, which we analysed with gas chromatography-mass spectrometry (GC-MS). Consistent with predictions, we found no sex difference in preen oil composition, neither in beta diversity (Bray-Curtis dissimilarities) nor in alpha diversity (Shannon index and number of substances). Based on these results, we cannot conclude whether preen oil has a function during incubation in Kentish plovers. Still, we discuss hypothetical roles, such as olfactory crypsis, protection against ectoparasites or olfactory intraspecific communication, which remain to be tested.
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Cromatografía de Gases y Espectrometría de Masas , Femenino , Masculino , Animales , Factores Sexuales , Caracteres SexualesRESUMEN
Males and females often have different roles in reproduction, although the origin of these differences has remained controversial. Explaining the enigmatic reversed sex roles where males sacrifice their mating potential and provide full parental care is a particularly long-standing challenge in evolutionary biology. While most studies focused on ecological factors as the drivers of sex roles, recent research highlights the significance of social factors such as the adult sex ratio. To disentangle these propositions, here, we investigate the additive and interactive effects of several ecological and social factors on sex role variation using shorebirds (sandpipers, plovers, and allies) as model organisms that provide the full spectrum of sex role variation including some of the best-known examples of sex-role reversal. Our results consistently show that social factors play a prominent role in driving sex roles. Importantly, we show that reversed sex roles are associated with both male-skewed adult sex ratios and high breeding densities. Furthermore, phylogenetic path analyses provide general support for sex ratios driving sex role variations rather than being a consequence of sex roles. Together, these important results open future research directions by showing that different mating opportunities of males and females play a major role in generating the evolutionary diversity of sex roles, mating system, and parental care.
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Evolución Biológica , Razón de Masculinidad , Conducta Sexual Animal , Medio Social , Animales , Femenino , Masculino , Conducta Sexual Animal/fisiología , Reproducción/fisiología , Charadriiformes/fisiología , Filogenia , Aves/fisiología , Rol de GéneroRESUMEN
Parental care can enhance offspring survival but may impose significant costs to parents. The costs and benefits of care are key to understanding patterns of parental care, where parents can benefit by having their partner increase investment in care, while reducing their own effort. However, investigating the costs and benefits of parental care in wild populations is challenging. Here we use highly detailed behavioral observations in families of a small shorebird, where one parent frequently deserts its offspring, to explore the potential costs and benefits of desertion in a wild population. We first show that females desert their broods more frequently than males. Second, we investigate the benefits of this frequent female desertion in terms of additional mating opportunities, and the costs of desertion to females in terms of the growth and survival of deserted offspring. Our results indicate that female desertion is favored by a combination of remating benefits and a lack of costs to brood growth and survival, as abandoned male parents continue to provide care after desertion. Our results shed light on the costs and benefits underlying natural desertion strategies and suggest that female desertion is a fine-tuned behavior that responds to seasonally changing benefits of desertion.
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Conducta Materna , Conducta Paterna , Animales , Humanos , Femenino , Masculino , Conducta Sexual Animal , Comportamiento de Nidificación , ReproducciónRESUMEN
The Eurasian Steppe belt is one of the largest biomes in the Northern Hemisphere. We provide here a range-wide phylogeography of the flightless steppe beetle Lethrus apterus that inhabits the western part of the Steppe belt through the study of population-level variance of mitochondrial cytochrome oxidase I sequences and nuclear microsatellites. We detected a concordant geographic structure of genetic data with a significant isolation-by-distance pattern. We found more genetic variation in the western part of the area and identified Northern Bulgaria and the Pannonian Basin as possible refugia. Genetic clusters were separated by main rivers in the eastern part of the area. This implies west-to-east colonisation and argues for an evolutionarily recent arrival of this species to its current main distribution area, the Pontic Steppes. This contradicts the classical biogeographical wisdom that assumed an east-to-west colonisation pattern.
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Escarabajos , Animales , Escarabajos/genética , ADN Mitocondrial/genética , Variación Genética , Pradera , Filogenia , FilogeografíaRESUMEN
Several members of the highly conserved oxytocin/vasopressin neuropeptide family are involved in the regulation of reproductive and affiliative behaviours in numerous vertebrate and invertebrate species. Here we investigate gene expression patterns of inotocin, the insect ortholog of this peptide family, and its receptor to decipher their possible role in the control of reproductive behaviour in a beetle, Lethrus apterus, with biparental care. In an experiment performed on individuals of a wild population, we found that inotocin is not related to the control of water balance in this species because expression patterns did not change as a response to drought exposure. The expression levels of inotocin and its receptor, however, increased over the reproductive season i.e., when behaviour shifts from pair formation to parental care, suggesting that inotocin might be involved in the regulation of parental care in this insect. No difference was, however, found between sexes; a finding which might indicate that inotocin plays a similar role in both parents.
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Escarabajos , Neuropéptidos/metabolismo , Oxitocina , Reproducción/fisiología , Animales , Escarabajos/metabolismo , Escarabajos/fisiología , Expresión Génica , Proteínas de Insectos/genética , Proteínas de Insectos/metabolismo , Insectos/metabolismo , Insectos/fisiología , Oxitocina/análogos & derivados , Oxitocina/genética , Oxitocina/metabolismo , Conducta Sexual Animal/fisiologíaRESUMEN
When individuals breed more than once, parents are faced with the choice of whether to re-mate with their old partner or divorce and select a new mate. Evolutionary theory predicts that, following successful reproduction with a given partner, that partner should be retained for future reproduction. However, recent work in a polygamous bird, has instead indicated that successful parents divorced more often than failed breeders (Halimubieke et al. in Ecol Evol 9:10734-10745, 2019), because one parent can benefit by mating with a new partner and reproducing shortly after divorce. Here we investigate whether successful breeding predicts divorce using data from 14 well-monitored populations of plovers (Charadrius spp.). We show that successful nesting leads to divorce, whereas nest failure leads to retention of the mate for follow-up breeding. Plovers that divorced their partners and simultaneously deserted their broods produced more offspring within a season than parents that retained their mate. Our work provides a counterpoint to theoretical expectations that divorce is triggered by low reproductive success, and supports adaptive explanations of divorce as a strategy to improve individual reproductive success. In addition, we show that temperature may modulate these costs and benefits, and contribute to dynamic variation in patterns of divorce across plover breeding systems.
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Evolución Biológica , Charadriiformes/fisiología , Reproducción/fisiología , Conducta Sexual Animal/fisiología , Animales , Cruzamiento , Divorcio , Femenino , Masculino , ApareamientoRESUMEN
The social environment is a key factor determining fitness by influencing multiple stages of reproduction, including pair formation, mating behavior and parenting. However, the influence of social structure across different aspects of breeding is rarely examined simultaneously in wild populations. We therefore lack a consolidation of the mechanisms by which sociality impacts reproduction. Here we investigate the implications of the social environment before and during breeding on multiple stages of reproduction in an island population of the ground nesting shorebird, the Kentish plover (Charadrius alexandrinus). We utilise information on mating decisions, nest locations and nesting success across multiple years in combination with social network analysis. Sociality before breeding was connected with patterns of pair formation. In addition, site fidelity and personal breeding experience was associated with the spatial organisation of breeding pairs. Our results provide evidence that, while differential social interactions at localised scales influence patterns of reproductive pairing, site fidelity and personal breeding experience influence the structure of populations at the landscape scale. Our results underline the tight link between the social structure of populations and patterns of mating, while revealing that the relative influence of sociality, breeding experience and local ecology are dynamic across different facets of reproduction.
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Cruzamiento , Charadriiformes/fisiología , Reproducción , Medio Social , Animales , Cabo Verde , Femenino , Masculino , Comportamiento de Nidificación , Conducta Sexual AnimalRESUMEN
Immunity and reproduction are physiologically demanding processes, therefore trade-offs are expected between these life history traits. Furthermore, investments in these traits are also known to be affected by factors such as sex, body size, individual condition, seasonal changes and parasite infection. The relationship between immunity and reproduction and the effect of other factors on this relationship were investigated in many species, but there are a small number of studies on these patterns in biparental invertebrates. Lethrus apterus is an iteroparous biparental beetle with predominant female care in respect of collecting and processing food for larvae. Males guard the nest built underground and also their mate. Here we investigate how sex, body size, time within the reproductive season and parasite load may influence the relationship between immunocompetence and reproductive investment in this species. In beetles from a natural population we quantified immune response by measuring the encapsulation response, antimicrobial activity of hemolymph, the investment into reproductive tissues by measuring the size of testis follicles in males and total egg size in females, and parasite load by counting the number of mites on the beetles. We found that the encapsulation response is condition-dependent, as large individuals showed significantly higher encapsulation ability than small ones. Antimicrobial capacity was significantly higher in females than in males. In case of antimicrobial activity there was also a seasonal change in the relationship between immunity and reproductive investment, but only under heavy mite load. Reproductive investment was influenced by the interaction between body size and season (in females) and by body size and season (in males). Furthermore in females the interaction between antimicrobial activity and season indicated that reproductive investment increased with antimicrobial activity early in the reproductive season. By investigating the relationship between immunity and reproductive investment in a natural population of a biparental beetle species, we can conclude that investments into these important life history traits are governed by complex interactions between physiological and environmental factors. Our results are discussed in the context of life history evolution, highlighting the role of the assessed factors in shaping trade-offs themselves (in invertebrates).
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Escarabajos/fisiología , Inmunidad/fisiología , Reproducción/ética , Animales , Tamaño Corporal , Ambiente , Femenino , Rasgos de la Historia de Vida , Masculino , Reproducción/fisiología , Estaciones del Año , Factores SexualesRESUMEN
Dogs (Canis familiaris) are excellent models of human behavior as during domestication they have adapted to the same environment as humans. There have been many comparative studies on dog behavior; however, several easily measurable and analyzable psychophysiological variables that are widely used in humans are still largely unexplored in dogs. One such measure is rapid eye movement density (REMD) during REM sleep. The aim of this study was to test the viability of measuring REMD in dogs and to explore the relationship between the REMD and different variables (sex, age, body size, and REM sleep duration). Fifty family dogs of different breeds and ages (from 6 months to 15 years old) participated in a 3-h non-invasive polysomnography recording, and the data for 31 of them could be analyzed. The signal of the electro-oculogram (EOG) was used to detect the rapid eye movements during REM sleep, and REMD was calculated based on these data. The duration of REM sleep had a quadratic effect on REMD. Subjects' REMD increased with age, but only in male dogs with short REM sleep duration. Furthermore, in the case of dogs with short REM sleep, the interaction of body mass and REM sleep duration had a significant effect on REMD. No such effects were found in dogs with long REM duration. These results suggest that relationships may exist between REMD and several different variables.
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Envejecimiento/fisiología , Tamaño Corporal/fisiología , Movimientos Oculares/fisiología , Caracteres Sexuales , Sueño REM/fisiología , Animales , Perros , Femenino , Masculino , Mascotas/fisiología , Polisomnografía , Factores de TiempoRESUMEN
The adult sex ratio (ASR) is a fundamental concept in population biology, sexual selection, and social evolution. However, it remains unclear which demographic processes generate ASR variation and how biases in ASR in turn affect social behaviour. Here, we evaluate the demographic mechanisms shaping ASR and their potential consequences for parental cooperation using detailed survival, fecundity, and behavioural data on 6119 individuals from six wild shorebird populations exhibiting flexible parental strategies. We show that these closely related populations express strikingly different ASRs, despite having similar ecologies and life histories, and that ASR variation is largely driven by sex differences in the apparent survival of juveniles. Furthermore, families in populations with biased ASRs were predominantly tended by a single parent, suggesting that parental cooperation breaks down with unbalanced sex ratios. Taken together, our results indicate that sex biases emerging during early life have profound consequences for social behaviour.
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Comunicación Animal , Conducta Cooperativa , Responsabilidad Parental/psicología , Reproducción/fisiología , Razón de Masculinidad , Animales , Charadriiformes , Femenino , Masculino , Modelos Biológicos , Caracteres SexualesRESUMEN
In resident-intruder contests, residents are expected to win more often than intruders unless the intruder has significantly higher competitive ability that is often determined by its size. Therefore, small intruders are expected to be less motivated to engage in contests than large ones and intruder size is predicted to have a positive relationship with the duration and escalation of the encounters. In a field experiment we tested these hypotheses in Lethrus apterus, a biparental beetle breeding in underground tunnels, by placing either a small or a large male in front of a tunnel occupied by a resident male. In agreement with our predictions residents won most of the encounters. Small intruders were less willing to engage in a contest and were less successful in their takeover attempts than large intruders. Contrary to many studies however, the duration and escalation (measured by the occurrence and latency of the different contest stages) of the fight in front of the burrow did not differ between the two intruder size categories. These findings suggest that in this species, small and large intruders adjust their prior decisions to their competitive abilities but once a fight started, they behave similarly during the contest.
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Agresión/psicología , Tamaño Corporal , Escarabajos , Motivación , Animales , Toma de Decisiones , MasculinoRESUMEN
Hormones play an important role in the regulation of physiological, developmental and behavioural processes. Many of these mechanisms in insects, however, are still not well understood. One way to investigate hormonal regulation is to analyse gene expression patterns of hormones and their receptors by real-time quantitative polymerase chain reaction (RT-qPCR). This method, however, requires stably expressed reference genes for normalisation. In the present study, we evaluated 11 candidate housekeeping genes as reference genes in samples of Lethrus apterus, an earth-boring beetle with biparental care, collected from a natural population. For identifying the most stable genes we used the following computational methods: geNorm, NormFinder, BestKeeper, comparative delta Ct method and RefFinder. Based on our results, the two body regions sampled (head and thorax) differ in which genes are most stably expressed. We identified two candidate reference genes for each region investigated: ribosomal protein L7A and RP18 in samples extracted from the head, and ribosomal protein L7A and RP4 extracted from the muscles of the thorax. Additionally, L7A and RP18 appear to be the best reference genes for normalisation in all samples irrespective of body region. These reference genes can be used to study the hormonal regulation of reproduction and parental care in Lethrus apterus in the future.
RESUMEN
The behavioural rhythms of organisms are thought to be under strong selection, influenced by the rhythmicity of the environment. Such behavioural rhythms are well studied in isolated individuals under laboratory conditions, but free-living individuals have to temporally synchronize their activities with those of others, including potential mates, competitors, prey and predators. Individuals can temporally segregate their daily activities (for example, prey avoiding predators, subordinates avoiding dominants) or synchronize their activities (for example, group foraging, communal defence, pairs reproducing or caring for offspring). The behavioural rhythms that emerge from such social synchronization and the underlying evolutionary and ecological drivers that shape them remain poorly understood. Here we investigate these rhythms in the context of biparental care, a particularly sensitive phase of social synchronization where pair members potentially compromise their individual rhythms. Using data from 729 nests of 91 populations of 32 biparentally incubating shorebird species, where parents synchronize to achieve continuous coverage of developing eggs, we report remarkable within- and between-species diversity in incubation rhythms. Between species, the median length of one parent's incubation bout varied from 1-19 h, whereas period length-the time in which a parent's probability to incubate cycles once between its highest and lowest value-varied from 6-43 h. The length of incubation bouts was unrelated to variables reflecting energetic demands, but species relying on crypsis (the ability to avoid detection by other animals) had longer incubation bouts than those that are readily visible or who actively protect their nest against predators. Rhythms entrainable to the 24-h light-dark cycle were less prevalent at high latitudes and absent in 18 species. Our results indicate that even under similar environmental conditions and despite 24-h environmental cues, social synchronization can generate far more diverse behavioural rhythms than expected from studies of individuals in captivity. The risk of predation, not the risk of starvation, may be a key factor underlying the diversity in these rhythms.
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Charadriiformes/fisiología , Comportamiento de Nidificación/fisiología , Periodicidad , Conducta Predatoria , Animales , Evolución Biológica , Charadriiformes/clasificación , Ritmo Circadiano , Señales (Psicología) , Ambiente , Conducta Alimentaria , Femenino , Masculino , Fotoperiodo , Reproducción , Especificidad de la Especie , Inanición/veterinaria , Factores de Tiempo , Cigoto/crecimiento & desarrolloRESUMEN
Sexual selection determines the elaboration of morphological and behavioural traits and thus drives the evolution of phenotypes. Sexual selection on males and females can differ between populations, especially when populations exhibit different breeding systems. A substantial body of literature describes how breeding systems shape ornamentation across species, with a strong emphasis on male ornamentation and female preference. However, whether breeding system predicts ornamentation within species and whether similar mechanisms as in males also shape the phenotype of females remains unclear. Here, we investigate how different breeding systems are associated with male and female ornamentation in five geographically distinct populations of Kentish plovers Charadrius alexandrinus. We predicted that polygamous populations would exhibit more elaborate ornaments and stronger sexual dimorphism than monogamous populations. By estimating the size and intensity of male (n = 162) and female (n = 174) melanin-based plumage ornaments, i.e. breast bands and ear coverts, we show that plumage ornamentation is predicted by breeding system in both sexes. A difference in especially male ornamentation between polygamous (darker and smaller ornaments) and monogamous (lighter and larger) populations causes the greatest sexual dimorphism to be associated with polygamy. The non-social environment, however, may also influence the degree of ornamentation, for instance through availability of food. We found that, in addition to breeding system, a key environmental parameter, rainfall, predicted a seasonal change of ornamentation in a sex-specific manner. Our results emphasise that to understand the phenotype of animals, it is important to consider both natural and sexual selection acting on both males and females.
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Desertion of clutch (or brood) by both parents often leads to breeding failure, since in vast majority of birds care by at least one parent is required for any young to fledge. Recent works in a highly polygamous passerine bird, the Eurasian penduline tit (Remiz pendulinus), suggest that biparental clutch desertion is due to intense sexual conflict over care. However, an alternative yet untested hypothesis for biparental desertion is low offspring viability so that the parents abandon the offspring that have poor prospect for survival. Here we test the latter hypothesis in a common garden experiment by comparing the viability of deserted and cared for eggs. We show that embryonic development does not differ between deserted and cared for eggs. Therefore, sexual conflict over care remains the best supported hypothesis for biparental clutch desertion in penduline tits. Our work points out that conflict over care is a potential - yet rarely considered - cause of biparental nest desertion, and a strong alternative for the traditional explanations of low offspring viability, human disturbance or deteriorating ambient environment. Apart from a handful of species, the intensity of sexual conflict has not been quantified, and we call for further studies to consider sexual conflict as a cause of nest desertion.
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Conducta Materna/fisiología , Comportamiento de Nidificación/fisiología , Passeriformes/fisiología , Conducta Paterna/fisiología , Animales , Femenino , Masculino , Apareamiento , Conducta Sexual AnimalRESUMEN
Previous research has suggested that owners' attitude to their family dogs may contribute to a variety of behaviour problems in the dog, and authors assume that dogs with separation-related disorder (SRD) attach differently to the owner than typical dogs do. Our previous research suggested that these dogs may have an insecure attachment style. In the present study we have investigated whether owners' attachment style, personality traits and the personality of the dog influence the occurrence of SRD in the dog. In an internet-based survey 1508 (1185 German and 323 Hungarian) dog-owners filled in five questionnaires: Demographic questions, Separation Behaviour Questionnaire (to determine SRD), Human and Dog Big Five Inventory and Adult Attachment Scale. We found that with owners' higher score on attachment avoidance the occurrence of SRD in the dog increases. Dogs scoring higher on the neuroticism scale were more prone to develop SRD. Our results suggest that owners' attachment avoidance may facilitate the development of SRD in dogs. We assume that avoidant owners are less responsive to the dog's needs and do not provide a secure base for the dog when needed. As a result dogs form an insecure attachment and may develop SRD. However, there may be alternative explanations of our findings that we also discuss.
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Perros/psicología , Vínculo Humano-Animal , Personalidad , Mascotas/psicología , Adolescente , Adulto , Anciano , Animales , Conducta Animal , Perros/fisiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Propiedad , Mascotas/fisiología , Encuestas y Cuestionarios , Adulto JovenRESUMEN
Sexual size dimorphism (SSD) among adults is commonly observed in animals and is considered to be adaptive. However, the ontogenic emergence of SSD, i.e. the timing of divergence in body size between males and females, has only recently received attention. It is widely acknowledged that the ontogeny of SSD may differ between species, but it remains unclear how variable the ontogeny of SSD is within species. Kentish Plovers Charadrius alexandrinus and Snowy Plovers C. nivosus are closely related wader species that exhibit similar, moderate (c. 4%), male-biased adult SSD. To assess when SSD emerges we recorded tarsus length variation among 759 offspring in four populations of these species. Tarsus length of chicks was measured on the day of hatching and up to three times on recapture before fledging. In one population (Mexico, Snowy Plovers), males and females differed in size from the day of hatching, whereas growth rates differed between the sexes in two populations (Turkey and United Arab Emirates, both Kentish Plovers). In contrast, a fourth population (Cape Verde, Kentish Plovers) showed no significant SSD in juveniles. Our results suggest that adult SSD can emerge at different stages of development (prenatal, postnatal and post-juvenile) in different populations of the same species. We discuss the proximate mechanisms that may underlie these developmental differences.
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The collembolan, Folsomia candida, is widely used in soil ecotoxicology. In recent years, growth rate of collembolans has become as frequently used endpoint as reproduction rate in ecotoxicological studies. However, measuring collembolan body sizes to estimate growth rate is a complicated and time-consuming task. Here we present a new image analysis method, which facilitates and accelerates the body length measurement of the collembolan Folsomia candida. The new software package, called CollScope, consists of three elements: 1) an imaging device; 2) photographing software; 3) an ImageJ macro for image processing, measurement and data analysis. We give a complete description of the operation of the software, the image analyzing process and describe its accuracy and reliability. The software with a detailed usage manual is attached as Supplementary Material. We report a case study to demonstrate that the automated measurement of collembolan body sizes is highly correlated with the traditional manual measurements (estimated measuring accuracy 0.05 mm). Furthermore, we performed a dose-response ecotoxicity test using cadmium-sulfate by using CollScope as well as classical methods for size measurement. Size data measured by CollScope or manually did not differ significantly. Furthermore the new software package decreased time consumption of the measurements to 42% when tested on 35 animals. Consequently, methodological investigations performed in this study should be regarded as a recommendation for any other routine dose-response study where body growth is an endpoint.
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Pesos y Medidas Corporales/métodos , Insectos/anatomía & histología , Animales , Pesos y Medidas Corporales/instrumentación , Ecotoxicología , Reproducibilidad de los ResultadosRESUMEN
Social behaviours are highly variable between species, populations and individuals. However, it is contentious whether behavioural variations are primarily moulded by the environment, caused by genetic differences, or a combination of both. Here we establish that biparental care, a complex social behaviour that involves rearing of young by both parents, differs between closely related populations, and then test two potential sources of variation in parental behaviour between populations: ambient environment and genetic differentiation. We use 2904 hours behavioural data from 10 geographically distinct Kentish (Charadrius alexandrinus) and snowy plover (C. nivosus) populations in America, Europe, the Middle East and North Africa to test these two sources of behavioural variation. We show that local ambient temperature has a significant influence on parental care: with extreme heat (above 40 °C) total incubation (i.e. % of time the male or female incubated the nest) increased, and female share (% female share of incubation) decreased. By contrast, neither genetic differences between populations, nor geographic distances predicted total incubation or female's share of incubation. These results suggest that the local environment has a stronger influence on a social behaviour than genetic differentiation, at least between populations of closely related species.