[Possibility of application of extracellular protease of micromycet Aspergillus ochraceus VKM F-4104D for determination of protein C content in human blood plasma]. / Vozmozhnost' primeneniia vnekletochnoi proteazy mikromitseta Aspergillus ochraceus VKM F-4104D dlia opredeleniia soderzhaniia proteina S v plazme krovi cheloveka.

It was shown that the activator activity of protein C, determined in normal plasma using Aspergillus ochraceus protease, is comparable with the activity of commercial protease analogue from the South American copperhead venom (ProtacÒ). It was found that protease of A. ochraceus can be used to determine protein C in plasma with its reduced content similar to ProtacÒ. Comparison of the activator protein C activity of A. ochraceus protease and the commercial analogue showed some excess of the activator activity of the fungal preparation, which may make it a promising substitute for the snake activator in diagnostical kits for determining the protein C content in clinical laboratories.

[Identification of Target Extracellular Proteases--Activators of Proteins of Haemostasis System Produced by Micromycetes Aspergillus ochraceus and Aspergillus terreus].

Effects of extracellular proteases of Aspergillus ochraceus and Aspergillus terreus on plasma hemostasis proteins, consist of initiating the activation of prothrombin complex proteins, was detected. Was discovered, that A. ochraceus proteases have a direct influence on protein C and coagulation factor X, and A. terreus proteases causes their activation indirectly through kallikrein system stimulation. The ability of extracellular proteases of micromycetes activate prekallikrein in human blood plasma on the example of A. terreus was first demonstrated.