Development and validation of a simple, sensitive enzyme immunoassay for quantification of androstenedione in bull plasma.

As an alternative to radioimmunoassay a simple and highly sensitive enzyme immunoassay (EIA) was developed and validated for androstenedione quantification in plasma of Karan Fries bulls using second antibody coating technique. The wells of the microtitreplate were coated with affinity-purified goat immunoglobulin (antirabbit IgG) that binds the hormone specific antibody. The EIA was performed to analyze androstenedione directly in 40 µl of bull plasma. The androstenedione standards ranged from 0.20 to 200 pg/40 µl /well and the sensitivity of the assay was 5 pg/ml plasma. Serially diluted bull plasma containing high endogenous androstenedione showed good parallelism with bovine androstenedione standard curve. Intra- and inter-assay coefficients of variation (CV) were found to be 8 and 9%, respectively. Peripheral plasma androstenedione concentrations determined in young and adult bull samples ranged between 104-990 pg/ml and 184-2040 pg/ml, respectively.

Characteristics of primary dentition occlusion in preschool children: an epidemiological study.

OBJECTIVE: The establishment and maintenance of normal occlusion constitutes one of the important objectives of pediatric dentistry. There are very few studies assessing the occlusal characteristics of primary dentition in the preschool children. The objective of this study was to assess the occlusal characteristics of primary dentition and difference in various parameters, in children between 3 and 5 years of age. MATERIALS AND METHODS: Eight hundred and eight healthy children, of age group between 3 and 5 years, and having full set of deciduous dentition, were selected by random sampling. Examination and recording of occlusal characteristics were done by single examiner. RESULTS: Flush terminal molar relationship was seen in 67.9% of children. Statistically significant (χ(2) = 47.835, p = 0.001) increase in mesial step molar relationship was seen with age. The class I canine relationship was the most prevalent canine relation, however the agewise changes of canine relation were not statistically significant. Anterior open bite was observed in 0.2% of children, which was less than the prevalence reported earlier in literature. 91.2% of children had overjet of 1 to 2 mm. The incidence of anterior and posterior crossbite was 0.4%. No cases of infraocclusion and scissors bite were observed. CONCLUSION: There is change in deciduous molar relationship as age increases. There is a significant increase in mesial step molar relation in 5 years age group compared to 3 years. The low prevalence of posterior crossbite and anterior open bite is suggestive of lower prevalence of sucking habits in children. Larger sample size may be required to assess the prevalence of infraocclusion and scissors bite. How to cite this article: Bhat SS, Rao HTA, Hegde KS, Kumar BSK. Characteristics of Primary Dentition Occlusion in Preschool Children: An Epidemiological Study. Int J Clin Pediatr Dent 2012;5(2):93-97.

Assessment of antidiarrhoeal activity of Desmostachya bipinnata L. (Poaceae) root extracts / Evaluación de la actividad de antidiarreica de extractos de la raíz de Desmostachya bipinnata L. (Poaceae)

Desmostachya bipinnata root has been used in the Indian traditional system of medicine for treatment of diarrhoea and dysentery. The antidiarrhoeal effect of both alcoholic and aqueous extracts of the roots of Desmostachya bipinnata were studied in rats against castor oil induced diarrhoea and charcoal meal test at the doses of 200 and 400 mg/kg body weight. The alcoholic extract and to a lesser extent aqueous extract significantly reduced the weight of the faces and decreased the propulsion of charcoal meal through the gastrointestinal tract. The phytochemical screening of the extracts showed the presence of alkaloids, glycosides, flavonoids, tannins, phytosterol, terpenoids, polyphenolics, protein and carbohydrates. These results may support the fact that this plant is used traditionally to cure diarrhoea.

La raíz de Desmostachya bipinnata ha sido utilizada en el sistema tradicional de medicina Hindú para el tratamiento de diarrea y disentería. El efecto antidiarreico de los extractos alcohólicos y acuosos de los extractos de la raíz de Desmostachya bipinnata fueron estudiados en ratas, utilizando la diarrea inducida por aceite de castor y el ensayo de la prueba por carbón en dosis de 200 y 400 mg/kg de peso corporal. El extracto alcohólico y en menor grado, el extracto acuoso, redujeron significativamente la propulsión de carbón a través del tracto gastrointestinal. El análisis de los extractos mostrarón la presencia de alcaloides, glicósidos, flavonoides, taninos, fitoesterol, terpenoides, polifenoles, proteínas y carbohidratos. Estos resultados pueden apoyar el hecho de que esta planta sea usada tradicionalmente para curar la diarrea.

Antideuteron yield at the AGS and coalescence implications

We present Experiment 864's measurement of invariant antideuteron yields in 11.5A GeV/c Au+Pt collisions. The analysis includes 250x10(6) triggers representing 14x10(9) 10% central interactions sampled for events with high mass candidates. We find (1/2pip(t))d(2)N/dydp(t) = 3.5+/-1.5(stat)+0.9-0.5(syst)x10(-8) GeV-2 c(2) for 1.8 = 0.35 GeV/c ( y(c.m.) = 1.6) and 3.7+/-2.7(stat)+1.4-1.5(syst)x10(-8) GeV-2 c(2) for 1.4 = 0.26 GeV/c, and a coalescence parameter B2; of 4.1+/-2. 9(stat)+2.3-2.4(syst)x10(-3) GeV2 c(-3). Implications for coalescence and antimatter annihilation are discussed.