RESUMEN
To meet the requirements of high-frequency ultrasound imaging systems, a transmit-beamforming integrated circuit with higher delay resolution than conventional transmit-beamforming circuits, which are typically implemented using field-programmable gate array chips, is presented. It also requires smaller volumes, allowing for portable applications. Its proposed design includes two all-digital delay-locked loops providing a specified digital control code for a counter-based beamforming delay chain (CBDC) to generate stable and suitable delays for exciting the array transducer elements without variations in process, voltage, and temperature. Moreover, to maintain the duty cycle of long propagation signals, this novel CBDC requires only a few delay cells, significantly reducing hardware costs and power consumption. Simulations were conducted, revealing a maximum time delay of 451.9 ns with a time resolution of 652 ps and a maximum lateral resolution error of 0.04 mm at 6.8 mm.
RESUMEN
Human pluripotent stem cells (hPSCs) are typically cultivated on extracellular matrix (ECM) protein-coated dishes in xeno-free culture conditions. We supplemented mixed ECM proteins (laminin-511 and recombinant vitronectin, rVT) in culture medium for hPSC culture on conventional polystyrene dishes. Three hPSC cell lines were successfully cultivated on uncoated polystyrene dishes in medium supplemented with optimal conditions of laminin-511 and rVT. Excellent colony shape and colony size as well as high expansion fold of hPSCs were found under these conditions, whereas the colony size was small and poor expansion fold was found solely on L-511-coated dishes. A small portion of L-511 in the culture medium supported hPSC adhesion and prevented the adhesion from being too strong on the uncoated dishes, and rVT in the culture medium further supported adhesion of hPSCs on the dishes by maintaining their pluripotency. Having the optimal composition of L-511 and rVT in the culture medium was important for generating good hPSC colony shapes and sizes as well as a high expansion fold. After long-term culture of hPSCs on uncoated dishes supplemented with the mixed proteins, the hPSCs successfully showed pluripotent markers and could differentiate into a specific lineage of cells, cardiomyocytes, with high efficiency.