Relação entre qualidade de vida e capacidade para o trabalho em funcionários do poder judiciário / Relationship between quality of life and capacity for work of employees of the judiciary

Contexto: Sabe-se que as doenças ocupacionais não prejudicam apenas a produtividade, por meio da diminuição da capacidade para o trabalho, mas também todos os aspectos relacionados à qualidade de vida do funcionário. Assim, a manutenção da capacidade para o trabalho relaciona-se com o estado de saúde para execução das atividades laborais. Essas, quando bem desenvolvidas, geram desfechos positivos para as organizações e os trabalhadores. Objetivo: Avaliar a relação entre qualidade de vida e capacidade de trabalho em servidores do Poder Judiciário. Métodos: Estudo observacional de corte transversal. Constituída por 88 servidores públicos do Poder Judiciário, de ambos os sexos, aleatorizados. Realizado no Tribunal de Justiça de Pernambuco, cidade do Recife. A coleta de dados ocorreu por meio de questionários, sobre o índice de capacidade para o trabalho, qualidade de vida e variáveis sociodemográficas. Para análise dos dados utilizou-se a correlação de Pearson. Resultados: 90,9% dos trabalhadores apresentaram uma renda de 5 ou mais salários mínimos, 73,9% trabalhavam até 6 horas por dia e 52,3% realizavam atividade física regular. Os trabalhadores apresentaram maiores correlações para capacidade do trabalho e qualidade de vida em praticantes de atividade física (R=0,60; p<0,001) e com menores horas de trabalho (R=0,61; p<0,001). Quanto ao sexo, ambos, feminino (R=0,62; p<0,001) e masculino (R=0,40; p<0,010), demonstraram relação moderada e significativa com qualidade de vida. Conclusão: A população de trabalhadores do Poder Judiciário do presente estudo apresentou correlação positiva quanto à capacidade para o trabalho e qualidade de vida geral, bem como nos domínios físico, social, psicológico e ambiental.

Background: As is known, occupational diseases hamper productivity by impairing not only the work ability, but also all the aspects related with the quality of life of employees. Maintenance of work ability is associated with the state of health needed to perform work activities, which when are properly done lead to satisfactory results for both organizations and workers. Objective: To investigate the relationship between quality of life and work ability among judicial employees. Methods: Cross-sectional observational study. The sample comprised 88 randomly selected judicial employees from both sexes. The study was conducted at the Court of Justice of Pernambuco, Recife, Brazil. Data collection was performed through questionnaires for sociodemographic variables, quality of life and Work Ability Index. Pearson's correlation was used for data analysis. Results: 90.9% of participants had income equivalent to 5 or more times the minimum wage; 73.9% worked up to 6 hours per day; and 52.3% reported regular practice of physical activity. Correlation between work ability and quality of life was stronger for the participants with regular practice of physical activity (R=0.60; p<0.001) and shorter working time (R=0.61; p<0.001). On analysis per sex, work ability exhibited significant and moderate correlation with quality of life for both males (R=0.62; p<0.001) and females (R=0.40; p<0.010). Conclusion: Relative to the analyzed sample of judicial employees positive correlation was found between work ability and overall quality of life, as well as with quality of life physical health, social, psychological and environment domains.

Relationship between quality of life and work ability among judicial employees.

BACKGROUND: As is known, occupational diseases hamper productivity by impairing not only the work ability, but also all the aspects related with the quality of life of employees. Maintenance of work ability is associated with the state of health needed to perform work activities, which when are properly done lead to satisfactory results for both organizations and workers. OBJECTIVE: To investigate the relationship between quality of life and work ability among judicial employees. METHODS: Cross-sectional observational study. The sample comprised 88 randomly selected judicial employees from both sexes. The study was conducted at the Court of Justice of Pernambuco, Recife, Brazil. Data collection was performed through questionnaires for sociodemographic variables, quality of life and Work Ability Index. Pearson's correlation was used for data analysis. RESULTS: 90.9% of participants had income equivalent to 5 or more times the minimum wage; 73.9% worked up to 6 hours per day; and 52.3% reported regular practice of physical activity. Correlation between work ability and quality of life was stronger for the participants with regular practice of physical activity (R=0.60; p<0.001) and shorter working time (R=0.61; p<0.001). On analysis per sex, work ability exhibited significant and moderate correlation with quality of life for both males (R=0.62; p<0.001) and females (R=0.40; p<0.010). CONCLUSION: Relative to the analyzed sample of judicial employees positive correlation was found between work ability and overall quality of life, as well as with quality of life physical health, social, psychological and environment domains.

CONTEXTO: Sabe-se que as doenças ocupacionais não prejudicam apenas a produtividade, por meio da diminuição da capacidade para o trabalho, mas também todos os aspectos relacionados à qualidade de vida do funcionário. Assim, a manutenção dacapacidade para o trabalho relaciona-se com o estado de saúde para execução das atividades laborais. Essas, quando bem desenvolvidas, geram desfechos positivos para as organizações e os trabalhadores. OBJETIVO: Avaliar a relação entre qualidade de vida e capacidade de trabalho em servidores do Poder Judiciário. MÉTODOS: Estudo observacional de corte transversal. Constituída por 88servidores públicos do Poder Judiciário, de ambos os sexos, aleatorizados. Realizado no Tribunal de Justiça de Pernambuco, cidade do Recife. Acoleta de dados ocorreu por meio de questionários, sobre o índice de capacidade para o trabalho, qualidade de vida e variáveis sociodemográficas. Para análise dos dados utilizou-se a correlação de Pearson. RESULTADOS: 90,9% dos trabalhadores apresentaram uma renda de 5 ou mais salários mínimos, 73,9% trabalhavam até 6 horas por dia e 52,3% realizavam atividade física regular. Os trabalhadores apresentaram maiores correlações para capacidade do trabalho e qualidade de vida em praticantes de atividade física (R=0,60; p<0,001) e com menores horas de trabalho (R=0,61; p<0,001). Quanto ao sexo, ambos, feminino (R=0,62; p<0,001) e masculino (R=0,40; p<0,010), demonstraram relação moderada e significativa com qualidade de vida. CONCLUSÃO: A população de trabalhadores do Poder Judiciário do presente estudo apresentou correlação positiva quanto à capacidade para o trabalho e qualidade de vida geral, bem como nos domínios físico, social, psicológico e ambiental.

A eficácia da estabilização segmentar vertebral no aumento do trofismo dos multífidos e melhora da dor em portadores de hérnia discal lombar / Efficacy of vertebral segmental stabilization in the increase of thophism of the multifidus and improvement of pain in individuals with lumbar disc herniation

Avaliar a eficácia da técnica de Estabilização Segmentar Vertebral (ESV) no aumento do trofismo dos multífidos e na melhora da dor em pacientes portadores de Hérnia Discal Lombar (HDL). MÉTODOS: Estudo do tipo série de casos, intervencionista não controlado. Participaram seis pacientes, com idade média de 41,1+7,47 anos, apresentando HDL em L4/L5 ou L5/S1. Os indivíduos foram submetidos a um exame Ultrassonográfico para avaliação do trofismo do multífido direito (medida latero-lateral e ântero-posterior), a uma avaliação da capacidade de recrutamento voluntário dos estabilizadores lombares (através de uma Unidade Pressórica de Biofeedback) e questionados quanto a presença e nível de dor (Escala Modificada de Borg). Realizou-se 15 sessões de ESV, 3 vezes por semana. A sessão era iniciada com os alongamentos ativos da coluna e membros inferiores e em seguida era aplicada a ESV que foi dividida em três etapas: cognitiva, associativa e automática. A primeira etapa objetivou treinar o recrutamento voluntário isolado dos estabilizadores lombares, promovendo a conscientização da contração através do uso da Unidade Pressórica de Biofeedback. A segunda fase compreendeu a co-contração dos estabilizadores lombares em diferentes posições e em situações dinâmicas, associadas aos movimentos dos membros inferiores e superiores. A terceira fase consistiu na ativação desses músculos em circuitos que exigiam atividades rápidas e explosivas de mudança de direção e posicionamento. No final de cada sessão era realizado um relaxamento associado à respiração.

Aim to evaluate the effectiveness of the Vertebral Segmental Stabilization technique in the increasing of the multifidus tropism and pain relief in patients with Lumbar disc Herniation (LDH). METHODOLOGY: case series study, interventionist not controlled. Six patients participated in this study aged 41.1 + 7.47 years with LDH at L4/L5 or L5/S1. Those individuals were submitted to an ultrasound examination to evaluate the trophism of right multifidus (measured lateral-lateral and anterior-posterior), there was an assessment of the capacity of voluntary recruitment of lumbar stabilizers (through a pressure biofeedback unit), they were also asked about the existence and level of pain (Modified Borg Scale). There were 15 sessions of VSS 3 times a week. Each session began with active stretching of the spine and lower limbs, and then it was applied ESV which was divided into three stages: cognitive, associative and automatic.. of lumbar stabilizers in different positions and dynamic situations, associated with the movement of the upper and lower limbs. The third phase consisted of the activation of those muscles in circuits that demanded activities quick and explosive changes of direction and positioning. At the end of each session it was carried out relaxation associated with breathing. RESULTS: We verified an increase in the size of right multifidus and improves the ability of voluntary recruitment of lumbar stabilizers in all participants, and reducing pain. CONCLUSION: The technique VSS is effective in increasing the trophism of the multifidus, in the improves ability to voluntary contraction and lumbar stabilizers effective in reducing pain symptoms. For complete pain relief, the findings suggest an association of VSS with other physiotherapy techniques.

Nucleoplasmic calcium regulates cell proliferation through legumain.

BACKGROUND & AIMS: Nucleoplasmic Ca(2+) regulates cell growth in the liver, but the proteins through which this occurs are unknown. METHODS: We used Rapid Subtraction Hybridization (RaSH) to subtract genes in SKHep1 liver cells expressing the Ca(2+) buffer protein parvalbumin (PV) targeted to the nucleus, from genes in cells expressing a mutated form of nuclear-targeted PV which has one of two Ca(2+)-binding sites inactivated. The subtraction permitted the selection of genes whose expression was affected by a small alteration in nuclear Ca(2+) concentration. RESULTS: The asparaginyl endopeptidase legumain (LGMN) was identified in this screening. When Ca(2+) was buffered in the nucleus of SKHep1 cells, LGMN mRNA was decreased by 97%, in part by a transcriptional mechanism, and decreased expression at the protein level was observed by immunoblot and immunofluorescence. Treatment with hepatocyte growth factor increased LGMN expression. Knockdown of LGMN by siRNA decreased proliferation of SKHep1 cells by ∼50% as measured both by BrdU uptake and mitotic index, although an inhibitor of LGMN activity did not affect BrdU incorporation. A significant reduction in the fraction of cells in G2/M phase was seen as well. This was associated with increases in the expression of cyclins A and E. Furthermore, LGMN expression was increased in hepatocellular carcinoma cells relative to normal hepatocytes in the same specimens. CONCLUSIONS: These findings suggest a new role for LGMN and provide evidence that nuclear Ca(2+) signals regulate cell proliferation in part through the modulation of LGMN expression. Increased expression of LGMN may be involved in liver carcinogenesis.

Influence of cardiopulmonary bypass on the plasma concentrations of atenolol.

BACKGROUND: Betablockers are used in the treatment of angina pectoris and others ischemic coronary diseases, reducing mortality and cardiovascular events. Atenolol is a hydrophilic betablocker which is characterized by gastrointestinal absorption, small extent of distribution and renal function-dependent elimination. OBJECTIVE: The study objective was to determine the inter-individual variability of atenolol in coronary patients. METHODS: Plasma atenolol was quantified in six blood samples collected during the preoperative period from seven patients with coronary insufficiency and surgical indication, chronically treated with atenolol PO 25 to 100 mg/day. All patients presented a normal or slightly reduced renal function. RESULTS: All enrolled patients presented normal or slightly reduced renal function as a result of age and underlying disease. Atenolol plasma concentrations showed a monoexponential decline, confirming the first-order pharmacokinetics at the doses employed for the control of coronary insufficiency (mean +/- SD): 123 +/- 56, 329 +/- 96, 288 +/- 898, 258 +/- 85, 228 +/- 79 and 182 +/- 73 ng/ml at times zero, 2, 4, 6, 8 and 12h after dose administration. The investigated group showed a small inter-patient variability of atenolol administrated at multiple regimens due to the hydrophilic characteristic of the drug. Furthermore, accumulation of atenolol administered chronically was greater in coronary patients, compared to healthy subjects. CONCLUSION: In view of its cardio-selectivity and low-variability, atenolol should be used as the first-choice drug for the treatment of acute coronary syndrome and other cardiovascular diseases.

Influência da circulação extracorpórea sobre as concentrações plasmáticas de atenolol / Influence of cardiopulmonary bypass on the plasma concentrations of atenolol

FUNDAMENTO: Os betabloqueadores são usados no tratamento da angina pectoris e outras doenças coronarianas isquêmicas, reduzindo mortalidade e eventos cardiovasculares. O atenolol é um betabloqueador hidrofílico, de absorção gastrointestinal, extensão de distribuição pequena e eliminação função renal-dependente. OBJETIVO: O objetivo deste estudo é o de determinar a variabilidade inter-individual do atenolol em pacientes coronarianos. MÉTODOS: Quantificou-se o atenolol plasmático em 6 amostras sangüíneas coletadas no pré-operatório de sete indivíduos portadores de insuficiência coronariana e indicação cirúrgica de revascularização do miocárdio, tratados cronicamente com atenolol, com doses diárias variando entre 25 a 100 mg PO. Todos os pacientes apresentavam função renal dentro da normalidade ou levemente reduzida. RESULTADOS: As concentrações plasmáticas obtidas evidenciaram decaimento monoexponencial, confirmando que o atenolol apresenta farmacocinética de primeira ordem nas doses empregadas para o controle da insuficiência coronariana grave (médias ± DP): 123 ± 56, 329 ± 96, 288 ± 898, 258 ± 85, 228 ± 79 e 182 ± 73 ng/mL, nos tempos zero, 2, 4, 6, 8 e 12 horas após a administração da dose. Registrou-se pequena variabilidade inter-pacientes nas concentrações plasmáticas de atenolol no grupo investigado tratado em regime de doses múltiplas, devido à característica hidrofílica do fármaco. Registrou-se ainda, maior persistência do atenolol nos pacientes coronarianos investigados, comparado a indivíduos saudáveis. CONCLUSÃO: Em virtude da sua cardioseletividade e baixa variabilidade, sugere-se que o atenolol deve ser empregado como fármaco de primeira escolha para o tratamento da síndrome coronariana aguda e outras doenças cardiovasculares.

BACKGROUND: Betablockers are used in the treatment of angina pectoris and others ischemic coronary diseases, reducing mortality and cardiovascular events. Atenolol is a hydrophilic betablocker which is characterized by gastrointestinal absorption, small extent of distribution and renal function-dependent elimination. OBJECTIVE: The study objective was to determine the inter-individual variability of atenolol in coronary patients. METHODS: Plasma atenolol was quantified in six blood samples collected during the preoperative period from seven patients with coronary insufficiency and surgical indication, chronically treated with atenolol PO 25 to 100 mg/day. All patients presented a normal or slightly reduced renal function. RESULTS: All enrolled patients presented normal or slightly reduced renal function as a result of age and underlying disease. Atenolol plasma concentrations showed a monoexponential decline, confirming the first-order pharmacokinetics at the doses employed for the control of coronary insufficiency (mean ± SD): 123 ± 56, 329 ± 96, 288 ± 898, 258 ± 85, 228 ± 79 and 182 ± 73 ng/ml at times zero, 2, 4, 6, 8 and 12h after dose administration. The investigated group showed a small inter-patient variability of atenolol administrated at multiple regimens due to the hydrophilic characteristic of the drug. Furthermore, accumulation of atenolol administered chronically was greater in coronary patients, compared to healthy subjects. CONCLUSION: In view of its cardio-selectivity and low-variability, atenolol should be used as the first-choice drug for the treatment of acute coronary syndrome and other cardiovascular diseases.

Avaliação da disposição cinética do atenolol em pacientes coronarianos submetidos à revascularização do miocárdio. Influência da circulação extracorpórea sobre as concentrações plasmáticas do atenolol no intra-operatório de cirurgia cardíaca / Evaluation of the kinetic disposition of atenolol in coronary patients submitted to coronary artery bypass grafting surgery. Influence of the cardiopulmonary bypass on the plasma concentrations of atenolol during the intra-operative period of cardiac

Pacientes submetidos à revascularização do miocárdio (RM), frequentemente utilizam betabloqueadores no pré-operatório para o controle da angina pectoris, e continuam o tratamento após a cirurgia, para a redução de mortalidade e complicações cardiovasculares perioperatórias. Entretanto, a circulação extracorpórea (CEC), empregada na maioria das cirurgias cardíacas, pode alterar as concentrações plasmáticas e a disposição cinética de muitos fármacos, e consequentemente seus efeitos terapêuticos. O atenolol é um beta-bloqueador altamente hidrossolúvel, de absorção incompleta e eliminação renal-dependente. O objetivo deste estudo foi o de investigar a influência da CEC sobre as concentrações plasmáticas do atenolol no intra-operatório de cirurgia cardíaca, além de comparar a sua farmacocinética no pré e pós-operatório de RM com CEC, em pacientes com angina instável grave. Investigou-se ainda, a variabilidade das concentrações plasmáticas do atenolol no período que antecede a cirurgia cardíaca. Na primeira etapa, avaliaram-se 19 pacientes coronarianos, em terapia crônica com atenolol PO, submetidos à cirurgia cardíaca com ou sem CEC. Na segunda parte, investigaram-se os períodos pré e pós-cirúrgico de 7 pacientes com angina instável, submetidos à RM com CEC e tratados com atenolol PO em regime de doses múltiplas. Todos os pacientes investigados apresentavam função renal dentro da normalidade ou leve disfunção renal, decorrente da idade e da insuficiência coronariana. O monitoramento do atenolol plasmático no intra-operatório de RM e o estudo farrnacocinético realizado antes e após a revascularização, exigiram coletas de amostras sangüíneas seriadas. A quantificação do atenolol em plasma foi realizada através da cromatografia líquida de alta eficiência com detector de fluorescência e consistiu num procedimento analítico rápido, simples e de baixo custo. Apenas 200 ‘mü’L de plasma foram utilizados em cada análise cromatográfica...

Disposição cinética do atenolol em pacientes coronarianos submetidos a revascularização do miocárdio / Kinetic disposition of atenolol in coronary patients submitted to the CABG surgery

A isquemia miocárdica é um importante fator de risco para a mortalidade e eventos cardiovasculares no perioperatório de cirurgias cardíacas e não-cardíacas, sendo que a administração profilática de 'beta'-bloqueadores nesse período, reduz estes riscos. Sabe-se que alterações fisiológicas ocorridas durante a cirurgia de revascularização do miocárdio (RM) com circulação extracorpórea (CEC) podem afetar as concentrações plasmáticas e a cinética de muitos fármacos. Neste estudo, investigou-se a farmacocinética do atenolol em pacientes com angina instável e sem prejuízo renal, submetidos à revascularização com CEC e em terapia crônica com atenolol peroral. O estudo farmacocinético exigiu coleta de amostras sangüíneas seriadas após as doses pré- e pós-operatória. Comparado ao pré-operatório, registrou-se redução não significativa no volume de distribuição e na depuração plasmática após a cirurgia, permanecendo inalterada a meia-vida biológica (p>0,05). Uma correlação linear negativa entre meia-vida e depuração pode ser estabelecida nos dois períodos do estudo (r: -0,77, p= 0,06 no pré-operatório e r: -0,89, p= 0,06 no pós-operatório), enquanto que se estimou correlação linear direta entre volume de distribuição e meia-vida biológica apenas no pré-cirúrgico (r: 0,54, p= 0,03 no pré-operatório e r: 0,09, p= 0,03 no pós-operatório). Conclui-se que a cirurgia de revascularização auxilia no restabelecimento da extensão da distribuição do atenolol

Myocardium ischemia is an important factor of risk for mortality and cardiovascular events in the perioperative period of cardiac and non cardiac surgeries. However, the prophylactic administration of b-blocker agents could reduce these risks. Physiologic changes, occurred during the coronary artery bypass graft (CABG) surgery with cardiopulmonary bypass (CPB), could alter plasma concentration and pharmacokinetics of many drugs. This study investigated the pharmacokinetics of atenolol in patients with unstable angina and without renal dysfunction, submitted to CABG surgery and treated chronically with atenolol PO. For pharmacokinetic analysis, 13 blood samples were collected after doses administrated pre- and post-operatively. Compared to the pre-operative period, it was verified a non-significant reduction in the apparent volume of distribution and plasma clearance after the surgery, remaining unchanged the biological half-life, p>0.05 (NS). A negative linear correlation between plasma clearance and elimination half-life was demonstrated in both periods of the study (r: -0.77 p=0.06, pre-surgery and r: -0.89, p=0.06, post-surgery), while a correlation between volume of distribution and biological half-life was established only before revascularization (r: 0,54 p= 0,03 , pre-surgery and r: 0,09, p=0,03, post-surgery). We suggest that the CABG surgery leads to the normalization of the extension of distribution of atenolol

A micromethod for the quantification of atenolol in plasma using high-performance liquid chromatography with fluorescence detection: therapeutic drug monitoring of two patients with severe coronary insufficiency before cardiac surgery.

A simple, rapid, selective, and sensitive analytical method was developed for the quantification of atenolol in small volumes of plasma, by high-performance liquid chromatography with fluorescence detection. Only 200 microL of plasma was used for chromatographic analysis. Separation was performed on a C18 reverse-phase column (4 microm) using a binary mobile phase consisting of 0.05 M of phosphate buffer, pH 5.5, and methanol (80:20, vol/vol) at a flow rate of 0.7 mL/minute. The retention times of atenolol and of the internal standard (sotalol) were 12.7 and 10.4 minutes, respectively. Validation of this analytical method showed a good linear correlation (8-2000 ng/mL), high sensitivity (quantification limit: 8 ng/ml and detection limit: 4 ng/mL), accuracy of 99.3%, and intraday and interday precision of 5.3% and 6.9%, respectively. Absolute recovery was 93.7%. The method was found to be robust, with acceptable stability. The analytical method was validated by the quantification of atenolol in plasma obtained from 2 patients with unstable angina, scheduled for myocardium revascularization surgery, who were chronically treated with 50 mg of atenolol administered per os once a day. The method developed was found to be adequate for use in pharmacokinetic studies and in adjusted dose pharmacotherapy.

Detection of human papillomavirus DNA by the hybrid capture assay.

Human Papillomavirus (HPV) infection is the main cause of cervical cancers and cervical intraepithelial neoplasias (CIN) worldwide. Consequently, it would be useful to evaluate HPV testing to screen for cervical cancer. Recently developed, the second-generation Hybrid Capture (HCA II) test is a non-radioactive, relatively rapid, liquid hybridization assay designed to detect 18 HPV types, divided into high and low-risk groups. We evaluated 1055 women for HPV infection with the HCA II test. Five hundred and ten (48.3%) of these women had HPV infection; 60 (11.8%) had low cancer-risk HPV DNA; 269 (52.7%) had high-risk HPV types and 181 (35.5%) had both groups. Hence, 450 women (88.2%) in this HPV-infected group had at least one high risk HPV type, and were therefore considered to be at high risk for cancer. Among the group with Papanicolaou (Pap) test results, the overall prevalence of HPV DNA was 58.4%. Significant differences in HPV infection of the cervix were detected between Pap I (normal smears) and Pap IV (carcinomas) (p<0.0001). Values of HPV viral load obtained for Pap I and SILs were significantly different, with an upward trend (p<0.0001), suggesting a positive correlation between high viral load values and risk of SIL. Because of the high costs of the HCA II test, its use for routine cervical mass screening cannot be recommended in poor countries. Nevertheless, it is a useful tool when combined with cytology, diagnosing high-risk infections in apparently normal tissues. Use of this technique could help reduce the risk of cancer.

Detection of human papillomavirus DNA by the hybrid capture assay

Human Papillomavirus (HPV) infection is the main cause of cervical cancers and cervical intraepithelial neoplasias (CIN) worldwide. Consequently, it would be useful to evaluate HPV testing to screen for cervical cancer. Recently developed, the second-generation Hybrid Capture (HCA II) test is a non-radioactive, relatively rapid, liquid hybridization assay designed to detect 18 HPV types, divided into high and low-risk groups. We evaluated 1055 women for HPV infection with the HCA II test. Five hundred and ten (48.3 percent) of these women had HPV infection; 60 (11.8 percent) had low cancer-risk HPV DNA; 269 (52.7 percent) had high-risk HPV types and 181 (35.5 percent) had both groups. Hence, 450 women (88.2 percent) in this HPV-infected group had at least one high risk HPV type, and were therefore considered to be at high risk for cancer. Among the group with Papanicolaou (Pap) test results, the overall prevalence of HPV DNA was 58.4 percent. Significant differences in HPV infection of the cervix were detected between Pap I (normal smears) and Pap IV (carcinomas) (p<0.0001). Values of HPV viral load obtained for Pap I and SILs were significantly different, with an upward trend (p<0.0001), suggesting a positive correlation between high viral load values and risk of SIL. Because of the high costs of the HCA II test, its use for routine cervical mass screening cannot be recommended in poor countries. Nevertheless, it is a useful tool when combined with cytology, diagnosing high-risk infections in apparently normal tissues. Use of this technique could help reduce the risk of cancer

Detecçäo de papilomavírus humanos pela técnica de captura híbrida, estudo preliminar / Detection of human papillomavirus infection by using the hybrid capture, study preliminary

No presente estudo foi determinado a prevalência da infecçäo por HPV pela técnica de captura híbrida em 217 pacientes atendidos pelo Laboratório de análises clínicas da rede privada do Rio de Janeiro. Destes, 184 eram do sexo feminino. Um total de 96 pacientes estavam infectadas por HPV, sendo que 80 apresentavam HPV de alto risco, constitutindo um grupo significativo com maior risco de transformaçäo maligna