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J Clin Microbiol ; 38(6): 2378-80, 2000 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-10835007

RESUMEN

This paper describes a new chromogenic plate medium, CHROMagar Staph aureus (CHROMagar, Paris, France), for the identification of Staphylococcus aureus on the basis of colony pigmentation. The abilities of CHROMagar Staph aureus, thermostable nuclease (DNase), and mannitol salt agar (MSA) to identify S. aureus isolates (n = 114) and discriminate between S. aureus and coagulase-negative staphylococci (CoNS; n = 22) were compared. CHROMagar Staph aureus proved to be more sensitive and specific than DNase and MSA, allowing a reliable, simple, and rapid method for the identification of S. aureus isolates. All CoNS encountered in this study with the exception of S. chromogenes could be easily differentiated from S. aureus on this medium. The supplementation with 4 microgram of oxacillin or methicillin per ml allowed simple identification of methicillin resistance in hospital-acquired S. aureus strains which show multiple-drug resistance profiles. Community-acquired methicillin-resistant S. aureus strains showing non-multi-drug resistance profiles require further evaluation on this new chromogenic medium. Methicillin or oxacillin resistance of all S. aureus isolates was confirmed by the detection of penicillin-binding protein 2a, encoded by the mecA gene, using the latex slide agglutination MRSA-Screen test (PBP 2' Test, DR900M; Oxoid).


Asunto(s)
Proteínas Bacterianas , Técnicas de Tipificación Bacteriana , Hexosiltransferasas , Resistencia a la Meticilina , Pruebas de Sensibilidad Microbiana/métodos , Peptidil Transferasas , Staphylococcus aureus/clasificación , Staphylococcus aureus/efectos de los fármacos , Proteínas Portadoras , Compuestos Cromogénicos , Coagulasa , Medios de Cultivo , Humanos , Muramoilpentapéptido Carboxipeptidasa , Proteínas de Unión a las Penicilinas , Pigmentación
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