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1.
ChemMedChem ; 15(4): 376-384, 2020 02 17.
Artículo en Inglés | MEDLINE | ID: mdl-31876113

RESUMEN

Glutamate racemases (GR) are members of the family of bacterial enzymes known as cofactor-independent racemases and epimerases and catalyze the stereoinversion of glutamate. D-amino acids are universally important for the proper construction of viable bacterial cell walls, and thus have been repeatedly validated as attractive targets for novel antimicrobial drug design. Significant aspects of the mechanism of this challenging stereoinversion remain unknown. The current study employs a combination of MD and QM/MM computational approaches to show that the GR from H. pylori must proceed via a pre-activation step, which is dependent on the enzyme's flexibility. This mechanism is starkly different from previously proposed mechanisms. These findings have immediate pharmaceutical relevance, as the H. pylori GR enzyme is a very attractive allosteric drug target. The results presented in this study offer a distinctly novel understanding of how AstraZeneca's lead series of inhibitors cripple the H. pylori GR's native motions, via prevention of this critical chemical pre-activation step. Our experimental studies, using SPR, fluorescence and NMR WaterLOGSY, show that H. pylori GR is not inhibited by the uncompetitive mechanism originally put forward by Lundqvist et al.. The current study supports a deep connection between native enzyme motions and chemical reactivity, which has strong relevance to the field of allosteric drug discovery.


Asunto(s)
Isomerasas de Aminoácido/antagonistas & inhibidores , Inhibidores Enzimáticos/farmacología , Helicobacter pylori/efectos de los fármacos , Simulación de Dinámica Molecular , Regulación Alostérica/efectos de los fármacos , Isomerasas de Aminoácido/metabolismo , Antibacterianos/química , Antibacterianos/farmacología , Relación Dosis-Respuesta a Droga , Inhibidores Enzimáticos/química , Helicobacter pylori/enzimología , Pruebas de Sensibilidad Microbiana , Estructura Molecular , Relación Estructura-Actividad
2.
Biochim Biophys Acta Gen Subj ; 1862(9): 1902-1912, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-29885360

RESUMEN

Glucokinase (GK) is an enzyme that catalyzes the ATP-dependent phosphorylation of glucose to form glucose-6-phosphate, and it is a tightly regulated checkpoint in glucose homeostasis. GK is known to undergo substantial conformational changes upon glucose binding. The monomeric enzyme possesses a highly exotic kinetic activity profile with an unusual sigmoidal dependence on glucose concentration. In this interdisciplinary study, which draws on small angle X-ray scattering (SAXS) integrated with 250 ns of atomistic molecular dynamics (MD) simulations and experimental glucose binding thermodynamics, we reveal that the critical regulation of this glucose sensor is due to a solvent controlled "switch". We demonstrate that the "solvent switch" is driven by specific protein structural dynamics, which leads to an enzyme structure that has a much more favorable solvation energy than most of the protein ensemble. These findings uncover the physical workings of an agile and flexible protein scaffold, which derives its long-range allosteric control through specific regions with favorable solvation energy. The physiological framework presented herein provides insights that have direct implications for the design of small molecule GK activators as anti-diabetes therapeutics as well as for understanding how proteins can be designed to have built-in regulatory functions via solvation energy dynamics.


Asunto(s)
Glucoquinasa/química , Glucoquinasa/metabolismo , Glucosa/metabolismo , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Mutación , Regulación Alostérica , Activación Enzimática , Glucoquinasa/genética , Humanos , Simulación de Dinámica Molecular , Proteínas Mutantes/genética , Unión Proteica , Conformación Proteica , Dispersión del Ángulo Pequeño , Relación Estructura-Actividad , Difracción de Rayos X
3.
Methods Enzymol ; 601: 243-273, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29523234

RESUMEN

Discovery of novel tool compounds and drug leads against a range of unorthodox protein targets has pushed both experimental screening methodologies as well as the field of structure-based design to the limit in recent years. Increasingly, it has been recognized that some of the most desirable targets for the development of small-molecule effectors are actually protein-protein and protein-nucleic acid interactions. There are numerous nontrivial challenges to pursuing small-molecule lead compounds directed toward PPIs and PNIs: relatively shallow cavities, large surface areas that are natively complexed to macromolecules, complex patterns of interstitial waters, a paucity of "hot spots," large conformational changes upon ligand binding, etc. Although there have been some notable successes targeting PPIs in the last decade, there has been distinctly less success in the realm of targeting PNIs. This chapter focuses on an approach, successfully applied by our group to address the challenge of gaining traction on the PPI target RAD52, which is a protein that binds both single-stranded and double-stranded DNA, and is an anticancer target for certain types of cancer. There are many approaches to tackling the difficult problems of finding effective small molecules that disrupt PPIs and PNIs, but the methods presented here offer a series of elegant solutions, which integrate experimental HTS, biophysical methods, docking, and molecular dynamics in a powerful way. Additionally, the structural knowledge gained from these studies provides a means for rationally understanding what features lead to ligand affinity in these fascinating and highly unorthodox pockets.


Asunto(s)
ADN/metabolismo , Simulación del Acoplamiento Molecular , Simulación de Dinámica Molecular , Proteína Recombinante y Reparadora de ADN Rad52/metabolismo , Humanos , Ligandos , Unión Proteica/efectos de los fármacos
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