RESUMEN
Guinea fowl coronavirus (GfCoV), a recently characterized avian coronavirus, was identified from outbreaks of fulminating disease (peracute enteritis) in guinea fowl in France. The full-length genomic sequence was determined to better understand its genetic relationship with avian coronaviruses. The full-length coding genome sequence was 26,985 nucleotides long with 11 open reading frames and no hemagglutinin-esterase gene: a genome organization identical to that of turkey coronavirus [5' untranslated region (UTR)-replicase (ORFs 1a, 1ab)-spike (S) protein-ORF3 (ORFs 3a, 3b)-small envelop (E or 3c) protein-membrane (M) protein-ORF5 (ORFs 4b, 4c, 5a, 5b)-nucleocapsid (N) protein (ORFs N and 6b)-3' UTR]. This is the first complete genome sequence of a GfCoV and confirms that the new virus belongs to group gammacoronaviruses.
Asunto(s)
Enfermedades de las Aves/virología , Infecciones por Coronavirus/veterinaria , Coronavirus/genética , Coronavirus/aislamiento & purificación , Genoma Viral , ARN Viral/genética , Análisis de Secuencia de ADN , Animales , Aves , Análisis por Conglomerados , Infecciones por Coronavirus/virología , Francia , Orden Génico , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Filogenia , Homología de Secuencia , SinteníaRESUMEN
Phylogenetic analyses based on the major capsid protein sequence indicate that Merkel cell polyomavirus (MCPyV) and chimpanzee polyomaviruses (PtvPyV1, PtvPyV2), and similarly Trichodysplasia spinulosa-associated polyomavirus (TSPyV) and the orangutan polyomavirus (OraPyV1) are closely related. The existence of cross-reactivity between these polyomaviruses was therefore investigated. The findings indicated serological identity between the two chimpanzee polyomaviruses investigated and a high level of cross-reactivity with Merkel cell polyomavirus. In contrast, cross-reactivity was not observed between TSPyV and OraPyV1. Furthermore, specific antibodies to chimpanzee polyomaviruses were detected in chimpanzee sera by pre-incubation of sera with the different antigens, but not in human sera.
Asunto(s)
Reacciones Cruzadas/inmunología , Pan troglodytes/virología , Filogenia , Infecciones por Polyomavirus/epidemiología , Poliomavirus/genética , Poliomavirus/inmunología , Animales , Proteínas de la Cápside/genética , Análisis por Conglomerados , Biología Computacional , Humanos , Italia/epidemiología , Microscopía Electrónica , Estudios Seroepidemiológicos , Virión/ultraestructuraRESUMEN
For decades, French guinea fowl have been affected by fulminating enteritis of unclear origin. By using metagenomics, we identified a novel avian gammacoronavirus associated with this disease that is distantly related to turkey coronaviruses. Fatal respiratory diseases in humans have recently been caused by coronaviruses of animal origin.