Optimizing ciprofloxacin removal through regulations of trophic modes and FNA levels in a moving bed biofilm reactor performing sidestream partial nitritation.

The performance of partial nitritation (PN)-moving bed biofilm reactor (MBBR) in removal of antibiotics in the sidestream wastewater has not been investigated so far. In this work, the removal of ciprofloxacin was assessed under varying free nitrous acid (FNA) levels and different trophic modes. For the first time, a positive correlation was observed between ciprofloxacin removal and FNA levels, either in the autotrophic PN-MBBR or in the mixotrophic PN-MBBR, mainly ascribed to the FNA-stimulating effect on heterotrophic bacteria (HB)-induced biodegradation. The maximum ciprofloxacin removal efficiency (∼98 %) and removal rate constant (0.021 L g-1 SS h-1) were obtained in the mixotrophic PN-MBBR at an average FNA level of 0.056 mg-N L-1, which were 5.8 and 51.2 times higher than the corresponding values in the autotrophic PN-MBBR at 0 mg FNA-N L-1. Increasing FNA from 0.006 to 0.056 mg-N L-1 would inhibit ammonia oxidizing bacteria (AOB)-induced cometabolism and metabolism from 10.2 % and 6.9 % to 6.2 % and 6.4 %, respectively, while HB-induced cometabolism and metabolism increased from 31.2 % and 22.7 % to 41.9 % and 34.5 %, respectively. HB-induced cometabolism became the predominant biodegradation pathway (75.9 %-85.8 %) in the mixotrophic mode. Less antimicrobial biotransformation products without the piperazine or fluorine were newly identified to propose potential degradation pathways, corresponding to microbial-induced metabolic types and FNA levels. This work shed light on enhancing antibiotic removal via regulating both FNA accumulation and organic carbon addition in the PN-MBBR process treating sidestream wastewater.

Inorganic carbon limitation decreases ammonium removal and N2O production in the algae-nitrifying bacteria symbiosis system.

Ammonium removal by a symbiosis system of algae (Chlorella vulgaris) and nitrifying bacteria was evaluated in a long-term photo-sequencing batch reactor under varying influent inorganic carbon (IC) concentrations (15, 10, 5 and 2.5 mmol L-1) and different nitrogen loading rate (NLR) conditions (270 and 540 mg-N L-1 d-1). The IC/N ratios provided were 2.33, 1.56, 0.78 and 0.39, respectively, for an influent NH4+-N concentration of 90 mg-N L-1 (6.43 mmol L-1). The results confirmed that both ammonium removal and N2O production were positively related with IC concentration. Satisfactory ammonium removal efficiencies (>98 %) and rates (29-34 mg-N gVSS-1 h-1) were achieved regardless of NLR levels under sufficient IC of 10 and 15 mmol L-1, while insufficient IC at 2.5 mmol L-1 led to the lowest ammonium removal rates of 0 mg-N gVSS-1 h-1. The ammonia oxidation process by ammonia oxidizing bacteria (AOB) played a predominant role over the algae assimilation process in ammonium removal. Long-time IC deficiency also resulted in the decrease in biomass and pigments of algae and nitrifying bacteria. IC limitation led to the decreasing N2O production, probably due to its negative effect on ammonia oxidation by AOB. The optimal IC concentration was determined to be 10 mmol L-1 (i.e., IC/N of 1.56, alkalinity of 500 mg CaCO3 L-1) in the algae-bacteria symbiosis reactor, corresponding to higher ammonia oxidation rate of ∼41 mg-N gVSS-1 h-1 and lower N2O emission factor of 0.13 %. This suggests regulating IC concentrations to achieve high ammonium removal and low carbon emission simultaneously in the algae-bacteria symbiosis wastewater treatment process.

Favipiravir biotransformation by a side-stream partial nitritation sludge: Transformation mechanisms, pathways and toxicity evaluation.

Information on biotransformation of antivirals in the side-stream partial nitritation (PN) process was limited. In this study, a side-stream PN sludge was adopted to investigate favipiravir biotransformation under controlled ammonium and pH levels. Results showed that free nitrous acid (FNA) was an important factor that inhibited ammonia oxidation and the cometabolic biodegradation of favipiravir induced by ammonia oxidizing bacteria (AOB). The removal efficiency of favipiravir reached 12.6% and 35.0% within 6 days at the average FNA concentrations of 0.07 and 0.02 mg-N L-1, respectively. AOB-induced cometabolism was the sole contributing mechanism to favipiravir removal, excluding AOB-induced metabolism and heterotrophic bacteria-induced biodegradation. The growth of Escherichia coli was inhibited by favipiravir, while the AOB-induced cometabolism facilitated the alleviation of the antimicrobial activities with the formed transformation products. The biotransformation pathways were proposed based on the roughly identified structures of transformation products, which mainly involved hydroxylation, nitration, dehydrogenation and covalent bond breaking under enzymatic conditions. The findings would provide insights on enriching AOB abundance and enhancing AOB-induced cometabolism under FNA stress when targeting higher removal of antivirals during the side-stream wastewater treatment processes.

Developing antibiotics-based strategies to efficiently enrich ammonia-oxidizing archaea from wastewater treatment plants.

The effects of five antibiotics (i.e., ampicillin, streptomycin, carbenicillin, kanamycin and tetracycline) on ammonia-oxidizing archaea (AOA) enrichment from anoxic activated sludge were investigated. The combined use of five antibiotics during 90-day cultivation could selectively inhibit nitrite-oxidizing bacteria (NOB) and ammonia-oxidizing bacteria (AOB) with AOA unaffected, as evidenced by the nitrite accumulation ratio of 100 % and the proportion of AOA in ammonia-oxidizing microbes over 91 %. The alternative use of five antibiotics was the optimal approach to screening for AOA during 348-day cultivation, which inhibited AOB growth at a level equivalent to the combined use of five antibiotics (the AOB-amoA gene decreased by over 99.90 %), further promoted AOA abundance (the much higher AOA-amoA to AOB-amoA gene copy number ratio (1453.30) than that in the groups with the combined use of five antibiotics (192.94)), eliminated bacterial adaptation to antibiotics and reduced antibiotic-resistant bacteria to form Nitrocosmicus-dominant community (42.35 % in abundance).

Establishing an efficient membrane bioreactor for simultaneous pollutant removal and purple bacteria production under salinity stress.

Recovering resources from wastewater to alleviate the energy crisis has become the prevailing trend of technological development. Purple phototrophic bacteria (PPB), a group of fast-growing microbes, have been widely noticed for their potential in producing value-added products from waste streams. However, saline contents in these waste streams, such as food processing wastewater pose a big challenge, which not only restrain the pollutant removal efficiency, but also hinder the growth of functional microbes. To overcome this, a photo anaerobic membrane bioreactor cultivating PPB (PPB-MBR) was constructed and its performance upon long-term salinity stress was investigated. PPB-MBR achieved desirable pollutants removal performance with the average COD and NH4+ removal efficiency being 87% (±8%, n = 87) and 89% (±10%, n = 87), respectively during long-term exposure to salinity stress of 1-80 g NaCl L-1. PPB were predominant during the entire operation period of 87 days (60%-80%), obtaining maximum biomass yield of 0.67 g biomass g-1 CODremoved and protein productivity of 0.18 g L-1 d-1 at the salinity level of 20 g NaCl L-1 and 60 g NaCl L-1, respectively. The sum of value-added products in proportion to the biomass reached 58% at maximum at the salinity level of 60 g NaCl L-1 with protein, pigments and trehalose contributing to 44%, 8.7%, and 5%, respectively. Based on economic analysis, the most cost-saving scenario treating food processing wastewater was revealed at salinity level of around 20 g NaCl L-1. However, more optimization tools are needed to boost the production efficiency so that the profit from value-added products can outweigh the additional cost by excess salinity in the future implication.

Modeling Free Nitrous Acid Inhibition on the Removal of Nitrogen and Atenolol during Sidestream Partial Nitritation Processes.

Sidestream serves as an important reservoir collecting pharmaceuticals from sludge. However, the knowledge on sidestream pharmaceutical removal is still insufficient. In this work, atenolol biodegradation during sidestream partial nitritation (PN) processes characterized by high free nitrous acid (FNA) accumulation was modeled. To describe the FNA inhibition on ammonia oxidation and atenolol removal, Vadivelu-type and Hellinga-type inhibition kinetics were introduced into the model framework. Four inhibitory parameters along with four biodegradation kinetic parameters were calibrated and validated separately with eight sets of batch experimental data and 60 days' PN reactor operational data. The developed model could accurately reproduce the dynamics of nitrogen and atenolol. The model prediction further revealed that atenolol biodegradation efficiencies by ammonia-oxidizing bacteria (AOB)-induced cometabolism, AOB-induced metabolism, and heterotrophic bacteria-induced biodegradation were 0, ∼ 60, and ∼35% in the absence of ammonium and FNA; ∼ 14, ∼ 29, and ∼28% at 0.03 mg-N L-1 FNA; and 7, 15, and 5% at 0.19 mg-N L-1 FNA. Model simulation showed that the nitritation efficiency of ∼99% and atenolol removal efficiency of 57.5% in the PN process could be achieved simultaneously by controlling pH at 8.5, while 89.2% total nitrogen and 57.1% atenolol were removed to the maximum at pH of 7.0 in PN coupling with the anammox process. The pH-based operational strategy to regulate FNA levels was mathematically demonstrated to be effective for achieving the simultaneous removal of nitrogen and atenolol in PN-based sidestream processes.

Eat seldom is better than eat frequently: Pharmaceuticals degradation kinetics, enantiomeric profiling and microorganisms in moving bed biofilm reactors are affected by feast famine cycle times.

Feast-famine (FF) regimes improved the removal of recalcitrant pharmaceuticals in moving bed biofilm reactors (MBBRs), but the optimal FF cycle remained unresolved. The effects of FF cycle time on the removal of bulk substrates (organic carbon and nitrogen) and trace pharmaceuticals by MBBR are systematically evaluated in this study. The feast to famine ratio was fixed to 1:2 to keep the same loading rate, but the time for the FF cycles varied from 18 h to 288 h. The MBBR adapted to the longest FF cycle time (288 h equaling 48 × HRT) resulted in significantly higher degradation rates (up to +183%) for 12 out of 28 pharmaceuticals than a continuously fed (non-FF) reactor. However, other FF cycle times (18, 36, 72 and 144 h) only showed a significant up-regulation for 2-3 pharmaceuticals compared to the non-FF reactor. Enantioselective degradation of metoprolol and propranolol occurred in the second phase of a two phase degradation, which was different for the longer FF cycle time. N-oxidation and N-demethylation pathways of tramadol and venlafaxine differed across the FF cycle time suggestin the FF cycle time varied the predominant transformation pathways of pharmaceuticals. The abundance of bacteria in the biofilms varied considerably between different FF cycle times, which possibly caused the biofilm to remove more recalcitrant bulk organic C and pharmaceuticals under long cycle times.

Transformation mechanisms of the antidepressant citalopram in a moving bed biofilm reactor: Substrate-depended pathways, eco-toxicities and enantiomeric profiles.

Citalopram (CIT) is one of the most consumed antidepressants and frequently detected in aquatic environments worldwide. Conventional wastewater treatment cannot remove this neuronal active pharmaceutical efficiently. Past studies showed that moving bed biofilm reactors (MBBRs) can degrade CIT but the exact transformation pathways and toxicity reduction remained unclear. In this study, the effects of substrate stimulation on CIT transformation in an MBBR were systematically investigated. The results showed that a co-metabolic stimulation by acetate increased the transformation rate by 54 % and 24 % at high (300 µg/L) and environmental concentration (1.8 µg/L) of CIT, respectively. Conversely, the complex substrates in raw wastewater reduced the reaction rates by 44 %, suggesting a competitive inhibition on the enzymatic sites. The substrate stimulation changed the enantiomeric fraction (EF) of CIT from racemic (EF=0.5) to 0.60 at the high CIT concentrations, while those at lower concentrations resulted in an EF of 0.33, indicating that probably different enantioselective enzymes degraded CIT at high concentrations than at low concentrations, i.e., the presence of 300 µg/L CIT was possibly sufficient to induce the synthesis of different enantioselective enzymes, than those originally present. Through non-target and target analysis, in total 19 transformation products (TPs) including 7 TPs that were hitherto not mentioned in the literature were identified. Among these were quaternary amines, alkenes and conjugate TPs. The major transformation pathways were a) nitrile hydrolysis (up to 43 %), b) amide hydrolysis, and c) N-oxidation. Dosing acetate up-regulated significantly the amide hydrolysis, N-oxidation and conjugation pathways but inhibited the N-demethylation and α-carbon hydroxylation pathways. The in-silico toxicity assessment of CIT and its TPs suggested the overall eco-toxic potential of TPs was reduced by MBBR. Furthermore, the degradation under carbon-limited (famine) conditions favored the formation of the more toxic carboxamide, N-desmethyl and alkene TPs, while carbon-rich conditions, promoted the production of the less toxic carboxylic acid, N-oxide and ester TPs. Therefore, this study demonstrated that a) the co-metabolic stimulation of CIT metabolization by dosing a simple carbon source or b) inhibition of CIT metabolization by complex substrates; c) substrate stimulation made a difference on CIT transformation rates, enantiomeric profiles, pathways and toxic potentials. Overall, a simple-carbon co-metabolic stimulated MBBR was an efficient up-regulation strategy to minimize hazardous CIT and CIT-TPs as much as possible.

Model-based assessment of mainstream nitrate/nitrite-dependent anaerobic methane oxidation and Anammox process in granular sludge at low temperature.

The process of nitrate/nitrite-dependent anaerobic methane oxidation (n-DAMO) coupled with anaerobic ammonium oxidation (Anammox) is one of groundbreaking discoveries for nitrogen removal and methane emission reduction from wastewater simultaneously. Yet its treatment of mainstream wastewater at low temperature is still a major challenge. In this work, a one-dimensional granular sludge model incorporating Arrhenius conversion for temperature effects was constructed to depict the relationships among n-DAMO microorganisms and Anammox. The model framework was successfully evaluated with 380 days measurement data from a membrane granular sludge reactor (MGSR) operated at temperature of 20-10 °C and fed with ammonium and nitrite. The model could satisfactorily predict the kinetics of nitrogen removal rates, effluent nitrogen concentrations and biomass fractions in MGSR at varying temperatures. Despite the decrease in microbial activity of functional microorganisms, the coupled n-DAMO and Anammox process based on granule system in mainstream wastewater treatment achieved a TN removal efficiency of about 98 % and a stable nitrogen removal rate of 0.55 g L-1 d-1. The model developed is expected to facilitate fundamentally understanding the underlying mechanisms of the coupled process and provide proposals for its practical engineering application in wastewater treatment plants.

Mechanistic studies on the effect of easy degradable carbon on pharmaceuticals removal in intermittently fed moving bed biofilm reactors.

This study was conducted to provide for the first time systematic data on how intermittent feeding with carbon (ethanol) affects the kinetics of pharmaceuticals degradation in a moving bed biofilm reactor (MBBR). The relationship between the degradation rate constants (K) of 36 pharmaceuticals and the length of famine was tested with 12 different feast-famine ratios: For 17 pharmaceuticals, intermittent feeding increased K with a factor of 3-17, while for six other pharmaceuticals, it decreased K. Concerning intermittent loading, three dependencies were detected: 1) for some compounds (e.g., valsartan, ibuprofen, iohexol), the K decreased linearly with carbon loading, 2) for three compounds (2 sulfonamides and benzotriazole) K increased linearly with carbon loading 3) for most compounds (e.g., beta blockers, macrocyclic antibiotics, candesartan, citalopram, clindamycin, gabapentin) K had a maximum around 6 d famine (with 2 d feast). Optimizing processes on MBBRs need therefore be conducted based on a prioritization of compounds.

Nitrogen removal by algal-bacterial consortium during mainstream wastewater treatment: Transformation mechanisms and potential N2O mitigation.

This work investigated nitrogen transformation pathways of the algal-bacterial consortium as well as its potential in reducing nitrous oxide (N2O) emission in enclosed, open and aerated reactors. The results confirmed the superior ammonium removal performance of the algal-bacterial consortium relative to the single algae (Chlorella vulgaris) or the activated sludge, achieving the highest efficiency at 100% and the highest rate of 7.34 mg N g MLSS-1 h-1 in the open reactor with glucose. Enhanced total nitrogen (TN) removal (to 74.6%) by the algal-bacterial consortium was achieved via mixotrophic algal assimilation and bacterial denitrification under oxygen-limited and glucose-sufficient conditions. Nitrogen distribution indicated that ammonia oxidation (∼41.8%) and algal assimilation (∼43.5%) were the main pathways to remove ammonium by the algal-bacterial consortium. TN removal by the algal-bacterial consortium was primarily achieved by algal assimilation (28.1-40.8%), followed by bacterial denitrification (2.9-26.5%). Furthermore, the algal-bacterial consortium contributed to N2O mitigation compared with the activated sludge, reducing N2O production by 35.5-55.0% via autotrophic pathways and by 81.0-93.6% via mixotrophic pathways. Nitrogen assimilation by algae was boosted with the addition of glucose and thus largely restrained N2O production from nitrification and denitrification. The synergism between algae and bacteria was also conducive to an enhanced N2O reduction by denitrification and reduced direct/indirect carbon emissions.

Unravelling ciprofloxacin removal in a nitrifying moving bed biofilm reactor: Biodegradation mechanisms and pathways.

Although moving bed biofilm reactors (MBBRs) have shown excellent antibiotic removal potentials, the information on underlying mechanisms is yet limited. This work assessed the removal of ciprofloxacin in an enriched nitrifying MBBR by clarifying the contribution of adsorption and microbial-induced biodegradation. Results demonstrated the considerable biomass adsorption (55%) in first 30 min. Limiting nitrite oxidizing bacteria growth or inhibiting nitrification would lead to lower adsorption capacities. The highest ciprofloxacin biodegradation rate constant was 0.082 L g SS-1 h-1 in the presence of ammonium, owing to ammonia oxidizing bacteria (AOB)-induced cometabolism, while heterotrophs played an insignificant role (∼9%) in ciprofloxacin biodegradation. The developed model also suggested the importance of AOB-induced cometabolism and metabolism over heterotrophs-induced biodegradation by analyzing the respective biodegradation coefficients. Cometabolic biodegradation pathways of ciprofloxacin mainly involved the piperazine ring cleavage, probably alleviating antimicrobial activities. It implies the feasibility of nitrifying biofilm systems towards efficient antibiotic removal from wastewater.

The mitigation effect of free ammonia and free nitrous acid on nitrous oxide production from the full-nitrification and partial-nitritation systems.

The potentials of using endogenous free ammonia (FA) and free nitrous acid (FNA) as nitrous oxide (N2O) mitigators were investigated in treatment of both mainstream and sidestream wastewater. Although the N2O emission factor of a sidestream partial-nitritation (PN) reactor (averaged 1.70 % ± 0.39 %, n = 30) was about 2.4 times higher than a mainstream full-nitrification (FN) reactor (averaged 0.72 % ± 0.24 %, n = 30) (P < 0.01), one-hour exposure of PN sludge to 1.5 mg HNO2-N/L FNA could virtually abolish N2O emission. As for FN sludge, both 45 mg NH3-N/L FA and 0.015 mg HNO2-N/L FNA successfully mitigated N2O production at varying dissolved oxygen (DO) levels (50 % vs 61 %), while 1.5 mg HNO2-N/L FNA not only reduced more N2O (92 %) but also altered the N2O dependency on DO. Both FNA and FA sludge treatment were effective N2O mitigation strategies with FNA toward the end of carbon neutrality and FA being more economically appealing (2 % cost saving).

Sartan blood pressure regulators in classical and biofilm wastewater treatment - Concentrations and metabolism.

Sartans are a group of pharmaceuticals widely used to regulate blood pressure. Their concentration levels were monitored in 80 wastewater treatment plants (WWTP) in the Baltic Sea Region, reached from limit of detection up to 6 µg/L. The concentrations were significantly different in different countries, but consistent within the respective country. The degradation of sartans (losartan, valsartan, irbesartan) in moving bed biofilm reactors (MBBRs) that utilize biofilms grown on mobile carriers to treat wastewater was investigated for the first time, and compared with the degradation in a conventional activated sludge (CAS) treatment plant. The results showed the formation of six microbial transformation products (TPs) of losartan, four of valsartan, and four of irbesartan in biological wastewater treatment. Four of these metabolites have not been described in the literature before. Chemical structures were suggested and selected TPs were verified and quantified depending on availability of true standards. Valsartan acid was a common TP of losartan, valsartan, and irbesartan. Losartan and irbesartan also shared one TP: losartan/irbesartan TP335. Based on the mass balance analysis, losartan carboxylic acid is the main TP of losartan, and valsartan acid is the main TP of valsartan during the biotransformation process. For irbesartan, TP447 is likely to be the main TP, as its peak areas were two orders of magnitude higher than those of all the other detected TPs of this compound. The effects of adapting biofilms to different biological oxygen demand (BOD) loading on the degradation of sartans as well as the formation of their TPs were investigated. Compared to feeding a poor substrate (pure effluent wastewater from a CAS), feeding with richer substrate (1/3 raw and 2/3 effluent wastewater) promoted the metabolism of most compounds (co-metabolization). However, the addition of raw wastewater inhibited some metabolic pathways of other compounds, such as from losartan/irbesartan to TP335 (competitive inhibition). The formation of irbesartan TP447 did not change with or without raw wastewater. Finally, the sartans and their TPs were investigated in a full-scale CAS wastewater treatment plant (WWTP). The removal of losartan, valsartan, and irbesartan ranged from 3.0 % to 72% and some of the transformation products (TPs) from human metabolism were also removed in the WWTP. However, some of the sartan TPs, i.e., valsartan acid, losartan carboxylic acid, irbesartan TP443 and losartan TP453, were formed in the WWTP. Relative high amounts of especially losartan carboxylic acid, which was detected with concentrations up to 2.27 µg/L were found in the effluent.

Effects of substrate loading on co-metabolic transformation pathways and removal rates of pharmaceuticals in biofilm reactors.

This study focused on the effects of substrate (raw wastewater) on the biological removal of 20 pharmaceuticals in moving bed biofilm reactors. This is the first study discriminating experimentally between effects of adaptation (45 d) and stimulation (100 h) on the removal of micropollutants. The results presented in this paper show: i) Tramadol and venlafaxine are subject to microbial N-oxidation (besides the known demethylation). ii) Changes in substrate loading, changed the preferential degradation pathways, e.g., from N-oxidation (under starvation) to N-demethylation of both model compounds: tramadol and venlafaxine, during adaptation and stimulation to high substrate supply. iii) In starving biofilms, the effects of stimulation on removal rates are minor (-100 to +150 %) in comparison to those caused by adaptation (-100 to +700 %). iv) Adaptation to high loadings resulted in increased removal rates (up to 700 % in selected cases) v) Adaptation to high loadings followed by high loading of stimulation, resulted in the highest increase of removal rates (+49 % to +1800 %) for hard-to-degrade compounds (e.g., diclofenac). All in all, this study shows that the efficiency of biofilm reactors is heavily dependent on their adaptation to substrate.

Free Nitrous Acid Inhibits Atenolol Removal during the Sidestream Partial Nitritation Process through Regulating Microbial-Induced Metabolic Types.

Limited studies have attempted to evaluate pharmaceutical removal during the sidestream partial nitritation (PN) process. In this work, atenolol biodegradation by PN cultures was investigated by maintaining ammonium and pH at different levels. For the first time, free nitrous acid (FNA), other than ammonium, pH, and free ammonia, was demonstrated to inhibit atenolol removal, with biodegradation efficiencies of ∼98, ∼67, and ∼28% within 6 days at average FNA levels of 0, 0.03, and 0.19 mg-N L-1, respectively. Ammonia-oxidizing bacteria (AOB)-induced metabolism was predominant despite varying FNA concentrations. In the absence of ammonium/FNA, atenolol was mostly biodegraded via AOB-induced metabolism (65%) and heterotroph-induced metabolism (33%). AOB-induced metabolism was largely inhibited (down to 29%) at 0.03 mg-N L-1 FNA, while ∼27 and ∼11% were degraded via heterotroph-induced metabolism and AOB-induced cometabolism, respectively. Higher FNA (0.19 mg-N L-1) substantially reduced atenolol biodegradation via heterotroph-induced metabolism (4%), AOB-induced metabolism (16%), and AOB-induced cometabolism (8%). Newly identified products and pathways were related to metabolic types and FNA levels: (i) deamination and decarbonylation (AOB-induced cometabolism, 0.03 mg-N L-1 FNA); (ii) deamination from atenolol acid (heterotrophic biodegradation); and (iii) nitro-substitution (reaction with nitrite). This suggests limiting FNA to realize simultaneous nitrogen and pharmaceutical removal during the sidestream process.

Biosorption of chromium (VI), iron (II), copper (II), and nickel (II) ions onto alkaline modified Chlorella vulgaris and Spirulina platensis in binary systems.

The simultaneous biosorption of chromium (VI), copper (II), iron (II), and nickel (II) was investigated by alkaline-modified Chlorella vulgaris and Spirulina platensis in binary systems. The alkaline modified biosorbents were CV-KCl, SP-KCl, CV-Na2CO3, and SP-Na2CO3. The maximum removal efficiency recorded in this study was 99.7% with a biosorbent dosage of 0.3 g within a pH range of 2 to 6. The highest biosorption capacities obtained were 14.1, 13.5, 21.6, and 15.8 mg/g for Cr (VI), Cu (II), Fe (II), and Ni (II), respectively. The pseudo-second-order best described the biosorption rate, while the Langmuir isotherm model best described the biosorption equilibrium interaction. The values for Gibbs free energy (ΔG°) were in the range of 0.5 to 6.5 kJ/mol (Cr-Fe), 1.3 to 8.4 kJ/mol (Cr-Ni), and 3.9 to 11.3 kJ/mol (Cr-Cu) binary systems. This showed that the biosorption processes were characterized by physisorption reactions. The Temkin constant B values were in the range of 0.339 to 1.485 kcal/mol and the biosorption processes were largely exothermic reactions. The values for the Freundlich constant KF were between 1.4 and 10.4 (L/g), which indicated favourable biosorption. The Temkin isotherm model confirmed a strong binding affinity for Fe (II) and Ni (II). The results suggest that potassium chloride and sodium carbonate modification are very suitable for green algae and cyanobacteria for the efficient removal of heavy metals.

Spectral bands of incandescent lamp leading to variable productivity of purple bacteria biomass and microbial protein: Full is better than segmented.

Purple non­sulfur bacteria (PNSB) are competent microorganisms capable of producing value-added products from waste streams. Light source is one of the most influential factors determining the efficiency of this process. Previous studies mostly focused on optimizing light intensity, while the impact of spectral bands on PNSB growth is still unknown. To fill the knowledge gap, this study investigated the responses of PNSB (i.e., Rhodobacter sphaeroides) growth, protein content and enzyme activity to various spectral bands of an incandescent lamp for the first time. It was found that the full spectrum of the incandescent lamp was propitious to cultivate PNSB than segmented spectral bands, as demonstrated by the maximum biomass yield of 1.05 g biomass g-1 CODremoved, specific growth rate of 0.53 d-1 and protein concentration of 0.48 g L-1. The production of biomass and protein under infrared (IR) spectral band were slightly lower than those under full spectrum, but 3.2 and 1.7 times higher than the average values (0.14 g L-1 and 0.07 g L-1) under visible spectral bands, respectively. The variation trends of enzymatic activities, such as fructose-1,6-bisphosphatase (FBP) and photopigments were consistent with that of PNSB biomass upon varying spectral bands, suggesting that the spectral bands might induce a variable PNSB biomass via affecting the Calvin cycle and photophosphorylation process. These results provide a new perspective that spectrum bands of light sources should be considered in the process optimization.

Insight into integration of photocatalytic and microbial wastewater treatment technologies for recalcitrant organic pollutants: From sequential to simultaneous reactions.

The more and more stringent environmental standards for recalcitrant organic pollutants pushed forward the development of integration of photocatalytic and microbial wastewater treatment technologies. The past studies proposed mainly two typical integration ways: a) Independent sequence of photocatalysis and biodegradation (ISPB) conducting the sequential reactions; b) Intimate coupling of photocatalysis and biodegradation (ICPB) conducting the simultaneous reactions. Although ICPB has received more attraction recently due to its novelty, ISPB gives an edge in certain cases. The article reviews the state-of-the-art ISPB and ICPB studies to comprehensively compare the two systems. The strengths and weaknesses of ISPB and ICPB regarding the treatment efficiency, cost, toxicity endurance and flexibility are contradistinguished. The reactor set-ups, photocatalysts, microbial characteristics of ISPB and ICPB are summarized. The applications for different kinds of recalcitrant compounds are elaborated to give a holistic view of the removal efficiencies and transformation pathways by the two technologies. Currently, in-depth understandings about the interference among mixed pollutants, co-existing components and key parameters in realistic wastewater are urgently needed. The long-term and large-scale application cases of the integration technologies are still rare. Overall, we conclude that both ISPB and ICPB technologies are reaching maturity while challenges still exist for two systems especially regarding the reliability, economy and generalization for realistic wastewater treatment plants. Future research should not only manage to reduce the cost and energy consumption by upgrading reactors and developing novel catalysts, but also attach importance to the cocktail effects of wastewater during the sequential or simultaneous photocatalysis and biodegradation.

Regulating light, oxygen and volatile fatty acids to boost the productivity of purple bacteria biomass, protein and co-enzyme Q10.

Purple non­sulfur bacteria (PNSB) possess significant potential for bioresource recovery from wastewater. Effective operational tools are needed to boost productivity and direct the PNSB biomass towards abundant value-added substances (e.g., protein and co-enzyme Q10, CoQ10). This study aimed to investigate the impact of light, oxygen and volatile fatty acids (VFAs) on PNSB growth (i.e., Rhodobacter sphaeroides) and productivity of protein and CoQ10. Overall, the biomass yields and specific growth rates of PNSB were in the ranges of 0.57-1.08 g biomass g-1 CODremoved and 0.48-0.71 d-1, respectively. VFAs did not influence the biomass yield, yet acetate and VFA mixtures enhanced the specific growth rate with a factor of 1.2-1.5 compared to propionate and butyrate. The most PNSB biomass (1.08 g biomass g-1 CODremoved and 0.71 d-1) and the highest biomass quality (protein content of 609 mg g-1 dry cell weight (DCW) and CoQ10 content of 13.21 mg g-1 DCW) were obtained in the presence of VFA mixtures under natural light and microaerobic (low light alternated with darkness; dissolved oxygen (DO) between 0.5 and 1 mg L-1) conditions (vs. light anaerobic and dark aerobic cultivations). Further investigation on VFAs dynamics revealed that acetate was most rapidly consumed by PNSB in the individual VFA feeding (specific uptake rate of 0.76 g COD g-1 DCW d-1), while acetate as a co-substrate in the mixed VFAs feeding might accelerate the consumption of propionate and butyrate through providing additional cell metabolism precursor. Enzymes activities of succinate dehydrogenase and fructose-1,6-bisphosphatase as well as the concentration of photo pigments confirmed that light, oxygen and VFAs regulated the key enzymes in the energy metabolism and biomass synthesis to boost PNSB growth. These results provide a promising prospect for utilization of fermented waste stream for the harvest of PNSB biomass, protein and CoQ10.