A two-compartment upflow pilot scale bioreactor system for microbial sulfate reduction control studies.

Souring in oil fields occurs mainly due to the activity of sulfate reducing bacteria (SRB). Most of the studies on SRB are performed using upflow packed-bed reactors that have a limitation to describe the region close to the injection wells in oil fields, which is characterized by void and saturated porous bed regions. Here, it is described the design and operation of a pilot scale system to investigate the SRB activity, inhibition and control in oil fields. •The bioreactor is composed by two-compartments (empty and packed-bed).•The reactor system has two parallel bioreactors that can be supplied with the same source of nutrients through a single pump or can be supplied separately with different solutions using two pumps.•The hydrodynamics for conventional packed bed bioreactors has a mixing behavior dependent of the flow rate and has a significant by-pass. In contrast, the two-compartment system presented here has a mixing behavior almost independent of the flow rate.

Detection and quantification of hepatitis E virus in the absence of IgG and IgM anti-HEV in HIV-positive patients.

AIMS: To improve RT-qPCR with an internal control and a synthetic standard curve to detect HEV in HIV co-infected patients. METHODS AND RESULTS: A single-stranded RNA (ssRNA) and a double-stranded DNA (dsDNA) synthetic curve were designed, compared to the international reference panel for HEV genotypes, and tested to quantify and detect a reference panel for HEV genotypes. The detection limit of the RNA synthetic curve (50 copies per ml) was better than the DNA synthetic curve (100 copies per ml) and the WHO standard curve (250 copies per ml). Then, 280 serum samples from HIV-positive patients were tested for HEV RNA, which was detected in 3·6% of serum samples. The viral load ranged from 2 × 102 copies per ml to 4·78 × 108 copies per ml. HEV IgM/IgG antibodies were not detected in the RNA-positive patients. Sequencing analysis of HEV showed that the virus belongs to genotype 3 (HEV GT3). CONCLUSIONS: Real-time PCR was a useful tool to estimate co-infection with HEV/HIV, even in patients with low viral loads and undetectable anti-HEV IgG and IgM antibodies. SIGNIFICANCE AND IMPACT OF THE STUDY: Hepatitis E virus genotype 3 (HEV GT3) has been associated with silent chronic hepatitis and cirrhosis in HIV-positive subjects worldwide, but there is a lack of data on this co-infection in Brazil.

Simulation of the inhibition of microbial sulfate reduction in a two-compartment upflow bioreactor subjected to molybdate injection.

Souring of oil fields during secondary oil recovery by water injection occurs mainly due to the action of sulfate-reducing bacteria (SRB) adhered to the rock surface in the vicinity of injection wells. Upflow packed-bed bioreactors have been used in petroleum microbiology because of its similarity to the oil field near the injection wells or production. However, these reactors do not realistically describe the regions near the injection wells, which are characterized by the presence of a saturated zone and a void region close to the well. In this study, the hydrodynamics of the two-compartment packing-free/packed-bed pilot bioreactor that mimics an oil reservoir was studied. The packed-free compartment was modeled using a continuous stirred tank model with mass exchange between active and stagnant zones, whereas the packed-bed compartment was modeled using a piston-dispersion-exchange model. The proposed model adequately represents the hydrodynamic of the packed-free/packed-bed bioreactor while the simulations provide important information about the characteristics of the residence time distribution (RTD) curves for different sets of model parameters. Simulations were performed to represent the control of the sulfate-reducing bacteria activity in the bioreactor with the use of molybdate in different scenarios. The simulations show that increased amounts of molybdate cause an effective inhibition of the souring sulfate-reducing bacteria activity.

Improved method for enumerating sulfate-reducing bacteria using optical density.

The photometric determination of bacterial concentration can be affected by secondary scattering and other interferences. The conventional growth medium for sulfate-reducing bacteria (SRB) has iron that precipitates as iron sulfides, a dark precipitate which is useful to indicate bacterial activity. However, iron hydroxides also precipitate at high pH values and the presence of these precipitates interferes considerably in the optical density of the solution affecting estimates of the cell population thus seriously limiting the use of the conventional method. In this method a modification of the current method improves the measurement of the optical density of a solution with SRB cells. •The method consists of an acidification with hydrochloric acid of a sample of a mixed culture of SRB enriched from the produced water from oil fields to pH below 2.•The results show that the relationship between the bacterial dry mass and absorbance is exponential in the observed range. It was observed a large slope in the linearized fit equation, and the acidified solution does not change the integrity of the SRB cells after the treatment.•The results of the kinetic experiments, including the bacterial growth time evolution, demonstrate the applicability of the method.

A kinetic study on bacterial sulfate reduction.

The activity of anaerobic sulfate reduction was studied using sulfate-reducing bacteria isolated from the water produced from a Brazilian oil reservoir. The effects of the initial sulfate concentration on the anaerobic sulfate reduction and sulfide generation kinetics were investigated. The redox potential, the biomass solution content, and the sulfate and the sulfide solution content were measured. The results indicate that the sulfate conversion and the sulfide generation are both first-order processes for the initial sulfate concentration of 823, 1,282, and 1,790 mg/L. The results for the kinetic constants for the sulfate conversion indicate an inhibition with the enhancement of the initial sulfate solution content. The kinetic constants for the sulfide generation indicate that this reaction is almost independent of the initial sulfate solution content due to the presence of at least two in-series processes that are faster than the microbial conversion of the sulfate. The kinetic test using the water from an onshore oil field, with an initial sulfide content of 228 mg/L and sulfate content of 947 mg/L, shows a sulfate conversion of 50 % in 528 h. The kinetic modeling for the net content of sulfate and sulfide indicates that the sulfate conversion is slower for this water than for the deionized water tests; however, the sulfide formation has almost the same conversion velocity. The reactions are first order in both cases.

Characterization of the lead smelter slag in Santo Amaro, Bahia, Brazil.

For 33 years, a primary lead smelter operated in Santo Amaro (Brazil). Since the 1970s, large amounts of Pb and Cd have been widely documented in the blood and hair of people living near the smelter. The plant closed down in 1993, and several years later, the Pb levels in the blood of children under 4 years of age living near the smelter were high, where the disposed lead slag was suspected to be the main source of this contamination. The objective of this study is to elucidate the source of the Pb contamination and any other potentially toxic contamination, focusing on the characterization of the slag. The samples used for this characterization study were taken from the slag heaps. The results of the chemical analysis showed that the major constituents of the slag, in decreasing order of wt%, were the following: Fe(2)O(3) (28.10), CaO (23.11), SiO(2) (21.39), ZnO (9.47), MgO (5.44), PbO (4.06), Al(2)O(3) (3.56), C (2.26), MnO (1.44), Na(2)O (0.27), S (0.37), K(2)O (0.26), and TiO(2) (0.25). The Cd content of the slag was 57.3mg/kg, which is relatively low. The X-ray diffraction and the electron probe microanalyzer X-ray mapping indicated that the major phases in the slag were wüstite, olivine, kirschsteinite, and franklinite. Only spheroidal metallic Pb was found in the slag. The leaching study showed that the slag was stable at a pH greater than 2.8, and only in an extremely acidic environment was the solubilization of the Pb enhanced significantly. The solubilization of Zn was very limited in the acidic and alkaline environments. These results can be explained by the limited leachability of the metallic Pb and Zn-bearing compounds. The leaching study used TCLP, SPLP, and SWEP and indicated that the lead slag was stable in weak acidic environments for short contact times.

Characterization and treatment of artisanal gold mine tailings.

The solid waste generated by artisanal gold mining, with high mercury and gold contents, can be found in several areas in the South America. The present study focused on the tailings of an artisanal gold mine area located in the Brazilian northeastern. Samples of the mine tailings were taken and used to perform a physical and chemical characterization study using X-ray diffraction, scanning electron microscopy, neutron activation, X-ray fluorescence, induced coupled plasma-mass spectrometry, among others analytical methods. The results indicate that the material is composed mainly by quartz and goethite, the characteristic size of the particles (d(50)) is about 150 microm, and the density is close of that of quartz. The main constituents are silicon, iron, and aluminum. The tailings gold content is of about 1.8 mg/kg and the mercury content is of about 10 mg/kg. A remarkable feature of this solid waste is that the gold and mercury are both concentrated in both the fine and the coarse particles, but not in particles of intermediary size. Leaching studies indicated that the tailings are stable in weak organic acids, but soluble in alkaline and aired cyanide solutions, in which 89% of gold and 100% of mercury are extracted in 24 h. Electroleaching experiments, performed using sodium chloride as electrolyte, indicated that mercury and gold are extracted simultaneously and the recovery of both metals can be as high as 70% in 4 h. In addition, chromium, nickel, and lead are found in relatively large amounts in the solution, which indicate an effectively action of the electroleaching method to clean up solid wastes contaminated with metals.

Efeitos de bifosfonatos e estatinas na reparação de fraturas em tíbias de ratas com osteoporose induzidacom dexametasona / Effects of bisphosphonates and statins on tibial bone healing in rats with osteoporosis induced by dexamethasone treatment

A osteoporose é uma doença caracterizada por diminuição da massa óssea, com consequente aumento da incidência de fraturas, necessitando de métodos preventivos e de tratamentos eficazes. Alguns estudos demonstram que não somente a osteoporose, mas fármacos como alendronato, promovem a não consolidação de fraturas, presumivelmente pelo desequilíbrio de atividade osteoblástica e osteoclástica ocasionadas por esse fármaco. O presente estudo teve como objetivo verificar, comparativamente, os efeitos de alendronato de sódio, risedronato sódico, atorvastatina cálcica e lovastatina na reparação de fraturas em ratas osteoporóticas induzidas com dexametasona. Os efeitos desses fármacos foram analisados pelos marcadores bioquímicos cálcio, fósforo, albumina, proteínas totais, fosfatase alcalina e exame histomorfométrico de densidade trabecular óssea. Além disso, foi realizada morfometria através das medidas de comprimento, espessura, diâmetro do calo ósseo e densidade das tíbias dos animais. Os resultados bioquímicos não apresentaram diferenças significativas. Através damorfometria e histomorfometria foi possível constatara indução da osteoporose com o glicocorticóide, assim como a melhoria na densidade óssea, densidade trabecular óssea e diâmetro do calo ósseo em todos os grupos tratados. Estas técnicas mostraram que a recuperação óssea foi equiparável ao do grupo normal, o que demonstra que os fármacos usados são eficazes em animais osteoporóticos submetidos à fratura, ressaltando que os melhores efeitos foram obtidos como alendronato.

Assessing flow alignment of nematic liquid crystals through linear viscoelasticity.

Shear alignment of rodlike nematic liquid crystals is found when the reactive parameter lambda>1. Measurements of lambda usually require complex experiments. This paper presents a method based on the nematodynamic theory of Leslie and Ericksen that assesses flow alignment through small amplitude oscillatory flow. The method is based on the fact that the effect of lambda on the storage modulus G' of linear viscoelasticity, when the director is along the flow direction, is directly proportional to lambda-1. Thus the alignment-nonalignment transition for increasing lambda is a reentrant viscoelastic transition: viscoelastic (lambda<1) -->purely viscous (lambda=0) -->viscoelastic (lambda>1) that is reflected in the storage modulus G' and in the "loss angle" delta= tan(-1) (G"/G'). The methodology is demonstrated by analyzing the Leslie-Ericksen equations for small-amplitude oscillatory Poiseuille flow of (4-n-octyl- 4' -cyanobiphenyl) (8CB) using analytical and scaling methods. Since linear viscoelastic moduli are easily accessible, the proposed methodology is an additional useful and economical tool for nematodynamicists.

Efeito de caseína, proteína isolada de soja e colestiramina em coelhos hiperlipidêmicos / Effect of casein, isolated protein of soy and cholestyramine in hyperlipidemics rabbits

As anormalidades lipídicas são de grande importância, devido a sua ligação com a doença vascular aterosclerótica e com a doença cardíaca coronariana. Assim, objetivou-se comparar os efeitos da ingestão da caseína e proteína de soja sobre a diminuição do colesterol e suas funções, triglicérides e glicose, deposição lipídica no tecido hepático e redução do peso corporal total. Coelhos da raça albina foram divididos durante 30 dias nos seguintes grupos: colesterol + ácido cólico + ração (hipercolesterolêmico) e ração pura (controle), um para caseína, um para proteimax (proteína isolada de soja) e um outro para a colestiramina. Os níveis de colesterol plasmático, triglicérides, LDL-c e HDL-c foram medidos, no 16º e 31º dias do experimento, utilizando kits enzimáticos e os resultados foram expressos em mg/dL. Foram analisadas amostras de tecido hepático, sendo os cortes fixados em historesina e paraplasto. Observou-se a indução significativa de hipercolesterolemia nos animais que receberam ração + colesterol + ácido cólico. Foi verificado um maior aumento de colesterol, LDL-c e triglicérides no grupo que recebeu caseína, bem como maior deposição de gotículas de gordura nos hepatócitos, em comparação com o grupo que recebeu proteimax. Em contrapartida, houve aumento proporcional aos níveis de colesterol total nos níveis de HDL para o grupo que recebeu proteimax. Observou-se, ainda, redução no peso corporal total desencadeado pela proteína de soja. Verificou-se que a ingestão de maior quantidade de proteína vegetal na dieta, em substituição a proteína animal, pode ser um fator de prevenção ao ganho de peso corporal, aumento do colesterol plasmático e à disposição de triacilgliceróis nos hapatócitos, levando à esteatose e à consequente degeneração hepática

Influência de produtos naturais na ativação da lipase pancreática humana / Influence of natural products in the human pancreatic lipase activation

O presente trabalho objetivou caracterizar a ação de alguns compostos naturais (baicaleína, quercitrina, isoquercitrina, bicina, xantona) sobre a lipase pancreática humana in vitro, na tentativa de elucidar seus mecanismos de ação relacionados com o catabolismo de triacilgliceróis. Na pesquisa com vários fármacos, que é o centro de nossos estudos, buscas com relação a mecanismo de ação estão relacionadas aos efeitos de substâncias teste sobre a atividade de enzimas. Assim, a atividade enzimática da lipase foi medida na presença de cada uma dessas substâncias, variando a concentração do substrato e fixando a concentração do agente, e variando a concentração do agente e fixando a concentração do substrato. Os resultados mostraram que todas as combinações testadas apresentaram ativação sobre a lipase, enquanto que a quercitrina na concentração de 1,6 x 10-5mol/L com um volume de substrato de 24,19 ml/L apresentou uma porcentagem de ativação maior (35,2%). Ao se fixar a concentração do substrato, foi observado que a bicina na concentração de 5,5 x 10-5 mol/L apresentou porcentagem de ativação maior (20,6%)