Thrombin Generation Assay and Hemostatic Profile for Elucidating Hypercoagulability in Endogenous Canine Hyperadrenocorticism.

Canine hyperadrenocorticism is a common endocrine disorder caused by chronic secretion of glucocorticoid, often associated with hypercoagulability and secondary thrombosis. The thrombin generation assay (TGA) evaluates hemostasis globally by measuring endogenous thrombin potential. We aimed to determine whether TGA is suitable for assessing hypercoagulability in dogs with endogenous hyperadrenocorticism (HAC), and to correlate TGA with coagulation markers including fibrinogen, antithrombin (AT), D-dimer, prothrombin time (PT) and activated partial thromboplastin time (aPTT), and with routine laboratory tests for elucidating prothrombotic mechanisms and evaluating their utility as hypercoagulability screening tests. Thrombin generation performed with high activator concentration showed significantly higher endogenous thrombin potential (ETP) (P = .0239) and peak thrombin (P = .0281) in Cushing patients. Fibrinogen (P = <.0001) and AT (P = .0444) activities were significantly higher in the HAC group, while those of PT (P = .0046) and aPTT (P = .0002) were lower. Basal cortisol levels correlated positively with fibrinogen (r = 0.4503; P = .0355) and negatively with AT activity (r = -0.4580; P = .0280). Fibrinogen and hematocrit values were inversely correlated (r = -0.4853; P = .0076). Our study confirmed the presence of higher thrombin generation in dogs with HAC. However, TGA performed with lower activator concentrations was unsuitable for detecting hypercoagulability. Higher AT and fibrinogen levels and lower aPTT activity were identified in dogs with HAC relative to controls suggesting a potential role for the combined use of these assays when assessing hypercoagulability in canine hyperadrenocorticism.

Evaluation of soft tissues simulant materials in cone beam computed tomography.

OBJECTIVES: To evaluate different materials in simulating soft tissues and to analyze the influence of these materials on the mean (MPIV) and standard deviation of pixel intensity values comparing them to a gold-standard in CBCT images. METHODS: Images of three piglet heads with their soft tissues intact (gold-standard) and different simulant materials were acquired: ice, modelling wax, and ballistic gelatin, with the same thickness of the original soft tissues. The pixel intensities were measured in dental, bone and soft tissues regions, in the mandible and maxilla, for all the groups. Analysis of variance, Dunnet's, Pearson's and linear regression tests were performed. RESULTS: The simulators did not significantly change the MPIV of teeth in comparison with the gold-standard (p = 0.1017). Only ice (p = 0.0156) affected the MPIV of bone. Wax (p = 0.001) and ice (p = 0.0076), but not ballistic gelatin (p = 0.5814), altered the MPIV of soft tissue regions. When assessing the influence of the location (mandible or maxilla) among the simulants, the differences were significant only for the soft tissue regions. Standard deviation was not influenced by simulants (p > 0.05), but ballistic gelatin presented the lower variability. CONCLUSIONS: The ballistic gelatin was the best soft tissue simulant since it had the lowest influence on the pixel intensity values for all regions.