Clinical diagnostic value of IL-14, 1L-16 and SAA in periodontitis.

OBJECTIVES: Periodontitis is a chronic infectious disease, which leads to inflammatory destruction of periodontal supporting tissues. Interleukin 14 (IL-14), Interleukin 16 (IL-16) and serum amyloid A (SAA) have been demonstrated to be abnormally expressed in inflammatory diseases. Therefore, this study was performed to analyzed the expression and potential clinical values of IL-14, 1L-16 and SAA in periodontitis. MATERIALS AND METHODS: A total of 100 periodontitis patients and 100 healthy volunteers were recruited and the saliva and serum samples were collected. Then the C-reactive protein (CRP), procalcitonin (PCT), IL-14, 1L-16 and SAA levels in the saliva and serum of periodontitis patients were measured by Elisa kits. Besides, the significance of CRP, PCT, IL-14, 1L-16 and SAA in periodontitis patients were analyzed by receiver operating characteristic (ROC) analysis. RESULTS: The results showed that CRP, PCT, IL-14, 1L-16 and SAA levels were significantly increased in the the saliva and serum of the periodontitis patients. Additionally, the area under the curve (AUC) of saliva CRP, PCT, IL-14, 1L-16 and SAA for the diagnosis of periodontitis were 0.9035, 0.9435, 0.9508, 0.9500 and 0.9467, respectively. The AUC of serum CRP, PCT, IL-14, 1L-16 and SAA for the diagnosis of periodontitis were 0.9035, 0.9435, 0.9508, 0.9500 and 0.9467, respectively. What's more, the diagnostic value of IL-14, 1L-16 and SAA were enhanced when combining with CRP and PCT. CONCLUSION AND CLINICAL RELEVANCE: This study demonstrated that IL-14, IL-16 and SAA expressions were upregulated in periodontitis patients and exhibited a significant significance for periodontitis diagnosis.

[Clinical effects of two different hydroxyapatite bone graft materials during maxillary sinus lifting surgery with bone grafting and simultaneous implantation of implants].

PURPOSE: To compare the clinical effects of two different hydroxyapatite bone graft materials in maxillary sinus lifting surgery with bone grafting and implantation at the same time. METHODS: Seventy-two patients with implantation in the maxillary posterior dental area and insufficient bone mass admitted to the First Affiliated Hospital of Bengbu Medical College were collected from March 2018 to April 2019, and they were divided into experimental group(n=36) and control group(n=36) by random number table. Patients in the control group used hydroxyapatite composite material, while patients in the experimental group used nano-hydroxyapatite composite material. The clinical effects, bone increment and bone density changes of the two groups of implants were compared, and postoperative implant stability and complications of the two groups were compared. Statistical analysis was performed with SPSS 18.0 software package. RESULTS: The survival rate of implants in the experimental group was significantly higher than that in the control group at 24 months (P＜0.05). Alveolar bone resorption in the experimental group was significantly lower than the control group 6 months after surgery(P＜0.05). The bone mineral density of implants in the experimental group was significantly higher than the control group 6 months and 12 months after operation(P＜0.05). The implant stability coefficient value of the experimental group was significantly higher than the control group 12 months after operation(P＜0.05). There was no significant difference in the total complication rate between the two groups(P＞0.05). CONCLUSIONS: In maxillary sinus lift, the survival rate of nano-hydroxyapatite composite material implanted in the same period is high, which can improve postoperative bone metabolism and increase implant stability with good safety.

A comparative histologic study of rapid three-dimensional zygomatic suture expansion osteogenesis.

OBJECTIVE: To explore the histologic changes in zygomatic suture with 3-dimensional (3D) zygomatic suture expansion osteogenesis (SEO). STUDY DESIGN: The zygomatic bones were drawn by 3D external expansion appliance, and sutures of the zygomatic were extended. Biopsy specimens of the zygomatic bone were collected after 1, 3, 5, and 8 weeks. Each specimen was stained with Triplex staining as well as hematoxylin and eosin (HE), and the histologic changes were evaluated compared with the control section. RESULTS: With the 1-week study group, there were visible fibroblasts, osteoblasts, and capillary vessels in the expanded suture tissues. The fibers were connected to the sides of suture in an orderly way. In the 3-week group, active bone formation can be seen in expanded sides. The bone trabeculae were matured and oriented in the direction of distraction in the expanded sides of suture and most of them were collagen fibers; but the tissues of both sides of expanded suture were not in order. New woven bones were found in the histologic sections of the 5-week group. In the 8-week group, reticular and elastic fibers could not be observed, and bones were formed completely. Compared with the histologic examination of the same tissue section and its change in different periods of the biopsy specimen with Triplex staining and HE staining, the method of Triplex staining showed more clearly in collagen, reticular, and elastic fiber examination. The superiority of HE staining was in showing fibrous tissue, shape of cells, and bone formation in various degrees of maturation in rapid SEO. It is suggested that the 2 staining methods might be combined in distinguishing the collagen as well as reticular fibers from fibrin in the same section simultaneously. CONCLUSIONS: Collagen fibers and new bones were observed to form rapidly in expanded sides with direct SEO. Continual affluent blood supply, integral structure of periosteum and synostoses in the suture, and rapid uniform calcification of the whole new tissues are the histologic characteristics of SEO.