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BMC Plant Biol ; 24(1): 245, 2024 Apr 05.
Artículo en Inglés | MEDLINE | ID: mdl-38575879

RESUMEN

Seed germination is an important development process in plant growth. The phytohormone abscisic acid (ABA) plays a critical role during seed germination. However, the mechanism of rapeseed in response to ABA is still elusive. In order to understand changes of rapeseed under exogenous ABA treatment, we explored differentially expressed metabolites (DEMs) and the differentially expressed genes (DEGs) between mock- and ABA-treated seedlings. A widely targeted LC-MS/MS based metabolomics were used to identify and quantify metabolic changes in response to ABA during seed germination, and a total of 186 significantly DEMs were identified. There are many compounds which are involved in ABA stimuli, especially some specific ABA transportation-related metabolites such as starches and lipids were screened out. Meanwhile, a total of 4440 significantly DEGs were identified by transcriptomic analyses. There was a significant enrichment of DEGs related to phenylpropanoid and cell wall organization. It suggests that exogenous ABA mainly affects seed germination by regulating cell wall loosening. Finally, the correlation analysis of the key DEMs and DEGs indicates that many DEGs play a direct or indirect regulatory role in DEMs metabolism. The integrative analysis between DEGs and DEMs suggests that the starch and sucrose pathways were the key pathway in ABA responses. The two metabolites from starch and sucrose pathways, levan and cellobiose, both were found significantly down-regulated in ABA-treated seedlings. These comprehensive metabolic and transcript analyses provide useful information for the subsequent post-transcriptional modification and post germination growth of rapeseed in response to ABA signals and stresses.


Asunto(s)
Brassica napus , Brassica rapa , Ácido Abscísico/farmacología , Ácido Abscísico/metabolismo , Plantones/metabolismo , Brassica napus/metabolismo , Cromatografía Liquida , Espectrometría de Masas en Tándem , Perfilación de la Expresión Génica , Germinación/genética , Brassica rapa/metabolismo , Metaboloma , Almidón/metabolismo , Sacarosa/metabolismo , Semillas , Regulación de la Expresión Génica de las Plantas , Transcriptoma
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