Exclusion of HDAC1/2 complexes by oncogenic nuclear condensates.

Nuclear condensates have been shown to regulate cell fate control, but its role in oncogenic transformation remains largely unknown. Here we show acquisition of oncogenic potential by nuclear condensate remodeling. The proto-oncogene SS18 and its oncogenic fusion SS18-SSX1 can both form condensates, but with drastically different properties and impact on 3D genome architecture. The oncogenic condensates, not wild type ones, readily exclude HDAC1 and 2 complexes, thus, allowing aberrant accumulation of H3K27ac on chromatin loci, leading to oncogenic expression of key target genes. These results provide the first case for condensate remodeling as a transforming event to generate oncogene and such condensates can be targeted for therapy. One sentence summary: Expulsion of HDACs complexes leads to oncogenic transformation.

Representation of the spatio-temporal narrative of The Tale of Li Wa.

This paper posits a framework of digital models integrating spatial narrative theories to represent the narrative and its experience of a Chinese classical novel, The Tale of Li Wa, which has been diversely interpreted by literary critics and historians for approximately 900 years. In the proposed framework of "Time-Space-time-Space," the spatio-temporal information derived from the text of the novel and its author is extracted and fused to map the instantaneous spatial pattern perceived by readers in the flow of read time, which helps contemporary readers re-understand the classical narrative and its context through a multi-possibilities, integrated, and in-depth approach. The paper presents one possible interpretation of the novel in the framework organized by the authors, illustrating the theme of growth with respect to the male protagonist in the capital Chang'an via the "what-how-why" model.