Physiological and toxicological response of Microcystis aeruginosa BCCUSP232 exposed to Salvinia auriculata extracts.

Microcystis aeruginosa is one of the most predominant freshwater bloom-forming cyanobacterium found globally which is capable of producing toxic secondary metabolites including microcystins that might intoxicate animals and humans when contaminated water or food is ingested. Salvinia auriculata Aubl is one of the plants that might possess bioactive compounds capable of controlling growth and reproduction of M. aeruginosa. The present study aimed to determine the presence of bioactive compounds in S. auriculata extracts and determine alterations occurred in growth and reproduction of M. aeruginosa when exposed to these plant extracts. In addition, this investigation aimed to examine the influence of S. auriculata on antioxidant enzymes detected in M. aeruginosa. The results obtained demonstrated that the aqueous and ethanolic extracts of S. auriculata presented potential for control of cyanobacteria populations, exhibiting algicidal action on M. aeruginosa as well as interfering in antioxidant enzymes activities and parameters associated with oxidative stress. Phytochemical analyses demonstrated the presence of polyphenols and flavonoids content in both extracts. In addition, application of S. auriculata extracts did not produce cytogenotoxicity and/or mutagenicity utilizing Allium cepa test. Therefore, further studies are needed in order to identify and characterize the compounds responsible for these effects on M. aeruginosa and provide information regarding the possible application of S. auriculata in the treatment of drinking water.

Duckweed application in nature-based system for water phytoremediation and high-value coproducts at family agrisystem from a circular economy perspective.

Duckweeds are widely recognized for their efficiency in the phytoremediation of agricultural and industrial effluents. This study had two main objectives: 1) Implement a Nature-based Solutions (NBS) utilizing the environmental services of duckweeds to improve water quality through phytoremediation in small fish farms; 2) Analysis of duckweeds biomass produced in these fish farms to develop coproducts from a circular economy perspective in family agrisystem in Brazilian Atlantic Forest. The effectiveness of the phytoremediation system was assessed by the reduction of the Trophic State Index (TSI). Phytoremediation in small fish farming NBS was implemented using Clarias gariepinus, employing two different managements approaches: (i) System I - L. minor cultured every 15 days, with biomass harvest and effluent analysis conducted in each cycle over 60 days; (ii) System II - L. minor cultured every 30 days, following a similar cycle and analysis. Additionally, effluent from fish production underwent testing for phytoremediation in a batch system within a climate-controlled laboratory. L. minor demonstrated efficiency in System II, leading to a reduction of the TSI. The dry biomass of the plants emerged as a viable source of amino acid for application in functional foods and feed or nutraceuticals. The findings underscore the potential integration of L. minor into the NBS system and the generation of new co-products from circular production. In addition to its effective phyto- remediation properties, L. minor's dry biomass exhibited appealing characteristics, with elevated levels of crude protein, minerals, fatty acids, and carotenoids. This positions L. minor as a promising candidate for developing bioproducts tailored for functional foods and nutraceuticals. This underscores the potential of duckweeds to produce valuable nutritional compounds beyond their remediation capabilities.

Cytogenotoxicity evaluation of heavy metals detected in extracts and infusion of Baccharis trimera, potential bioaccumulator plant.

Heavy metals (HMs) are natural components of the Earth's crust that might originate from natural and anthropogenic sources. In excess quantities, the presence of these metals is harmful for both environment and human health. Taking this into account, various investigators examined bioaccumulator species in order to reduce environmental toxicity, among these Baccharis trimera. Therefore, the present study aimed to determine the capacity of B. trimera to bioaccumulate HMs and assess consequent cytogenotoxicity following exposure. B. trimera vegetative parts were collected from two groups (1) control, in which plants were cultivated in soil exposed to distilled water, and (2) exposed, in which plants were cultivated in soil exposed to HMs including manganese (Mn), iron (Fe), lead (Pb), copper (Cu), cobalt (Co), zinc (Zn), and chromium (Cr). HMs were quantified in cultivation soil and extracts (aqueous and ethanolic) as well as infusion of B. trimera vegetative parts. Root lengths and cytogenotoxic effects were determined using Allium cepa test. Results demonstrated that all HMs studied were absorbed and bioaccumulated by B. trimera. Root lengths were decreased when exposed to ethanolic extract of B. trimera cultivated in soil exposed to HMs solution, which was the extract that exhibited the highest cytogenotoxicity values. Thus, data demonstrated that B. trimera might serve as a bioaccumulator for the reduction of environmental toxicity associated with the presence of certain HMs.

Cytogenotoxic, insecticidal, and phytotoxic activity from biomass extracts of the freshwater algae Nitella furcata.

Agroecology, the application of ecological concepts to agricultural production, has been developing over the last years with consequent promotion for discovery of bioactive compounds to control pests and abolish crop diseases. In this context, algae from Nitella genus are characterized by high potential for bioeconomic applications due to (1) available biomass for harvesting or cultivation and (2) production of allelochemicals, which present a potential to protect field crops from insect infestation. Therefore, this study aimed to determine primary and secondary metabolites derived from aqueous and hydroethanolic extracts of Nitella furcata and to evaluate phytotoxic, cytogenotoxic, insecticidal, and pro-oxidative activities of these extracts. Determination of metabolites showed the presence predominantly of carbohydrates, proteins, phenols, and flavonoids in hydroethanolic extract. Both extracts of N. furcata interfered in the germination of seeds and development of seedlings of Lactuca sativa, with hydroethanolic extract exhibiting greater inhibition. Both extracts also interfered with meristematic cells of Allium cepa as evidenced by chromosomal alterations and higher pro-oxidative activity. Aqueous extract at 5 and 0 mg/ml produced 100% insect mortality. Further, hydroethanolic extract at 0 mg/ml was lethal immediately upon exposure. Therefore, results demonstrate that N. furcata is potential algae species to be considered for development of environmental and ecotoxicological studies as a source of compounds with potential use in agroecological strategies.

Coastal degradation impacts on green turtle's (Chelonia mydas) diet in southeastern Brazil: Nutritional richness and health.

This study evaluated the influence of environmental degradation on the nutritional value of the main marine macrophytes consumed by green sea turtles (Chelonia mydas) in areas with different degrees of urbanization. Macrophyte assemblages in the highly urbanized area (HUa) showed lower richness compared to the lightly urbanized area (LUa) (Mann-Whitney U test: 10.0 ± 3.6 SD genera and 11.9 ± 4.2 taxa per transect vs. 20.1 ± 7.0 genera and 23.5 ± 9.2 taxa per transect) respectively. Also, diet was poorer with 4.0 ± 1.6 genera per turtle (vs. 8.5 ± 4.0 in HUa) and less diverse with Shannon index of diversity = 0.45 ± 0.29 (vs. 0.64 ± 0.46 in LUa). Body condition was similar in both areas. About half of individuals were classified as having normal body condition, 14-15% as underweight and 23-34% as being emaciated. Fibropapillomatosis prevalence (χ2 = 8.720; n = 222; df = 1; p = 0.003) was higher in the HUa but, in affected animals, severity was marginally non-significant (χ2 = 5.721; n = 82; df = 2; p = 0.057). Significant differences in energy content (kcal) were detected between areas in both summer (S) and winter (W). All ANOVAs on total lipids (F = 22.15 [S] and 30.39 [W]), total water-soluble proteins (F = 327.65 [S] and 64.42 [W]) and total carbohydrates (F = 70.90 [S] and 27.62 [W]) showed high significance (p < 0.001). Carotenoids concentration yielded significant results for Halodule in summer and Hypnea in winter (ANOVAs, F = 39.42 and 13.07, respectively). For both, tests revealed that concentration was higher in LUa than HUa. High levels of phycobiliproteins and proteins in this area probably reflect nitrogen accumulation. Frequency and severity of fibropapillomatosis suggest that urbanization-caused alterations in species diversity and in chemical composition of marine plants affect green turtles' health. LIGHT ABSTRACT: The use of coastal areas by humanity is widespread and increasing. The impacts caused to the coastal environment, be it terrestrial, estuarine or marine, are important and affect numerous species. Our study evaluated the influence of environmental degradation on the nutritional value of the main algae eaten by the green turtle, one of the very few marine megaherbivores (those herbivores with body mass above 10 kg). Diet in the highly urbanized area was richer in proteins, lipids and carbohydrates (sugars) and lower in carotenoids (photosynthetic and photoprotectant pigments in algae and plants; precursors of vitamin A involved in oxygen transport in animals-animals do not synthetize such molecules). High levels in phycobiliproteins (photosynthetic pigments present in some algae) and proteins in the highly urbanized area probably result from organic pollution and nitrogen accumulation in coastal waters. Nitrogen compounds dissolved in water are a threat to vertebrates due to its toxicity and negative effects on the immune system. Our results suggest that algae chemical composition and severity of fibropapillomatosis (tumors caused by a herpesvirus in green sea turtles) are directly related through environmental alterations caused by urbanization.

Bone marrow morphologic features, MyPRS, and gene mutation correlations in plasma cell myeloma.

Genetics has played an important role in risk stratification for plasma cell myeloma patients, providing therapeutic guidance. In this study, we investigated the correlation of bone marrow morphologic features and genetic aberrations, including gene expression profiles, translocations, and gene mutations. For the first time we show that high plasma cell volume, diffuse sheet growth pattern, immature cell morphology, high mitotic index, and increased reticulin fibrosis, significantly correlates with high risk disease determined by MyPRS gene expression profiles. Furthermore, we show the association between MyPRS risk stratification and chromosomal alterations and specific gene mutations. We also demonstrate the combinational effect of TP53 mutation and 17p loss on the histological changes in bone marrow.

Antischistosomal activity from Brazilian marine algae

Abstract Schistosomiasis may be caused by six different species of the genus Schistosoma. Current treatment is based only on two drugs: oxamniquine, which is only effective against the Schistosoma mansoni species, and praziquantel, which is ineffective against young parasites. Therefore, research on new drugs and their targets for the treatment of this disease is urgently needed. In the present work, the efficacies of several seaweeds extracts against S. mansoni were tested. Worm couples were incubated with different concentration of seaweed extracts for 120 h and monitored after the first 2 h and then every 24 h to evaluate death, mobility reduction and couple detachment. The extracts of 13 different seaweed species were tested in a first trial and the active extracts were further evaluated in lower concentrations. The extracts of Gracilaria ornata and species belonging to the genera Dictyota and Laurencia showed activity at relatively low concentrations. The active extracts were analyzed by LC–MS, and possible candidates are proposed.

Evaluation of acetylcholinesterase inhibitory activity of Brazilian red macroalgae organic extracts

Abstract Alzheimer's disease affects nearly 36.5 million people worldwide, and acetylcholinesterase inhibition is currently considered the main therapeutic strategy against it. Seaweed biodiversity in Brazil represents one of the most important sources of biologically active compounds for applications in phytotherapy. Accordingly, this study aimed to carry out a quantitative and qualitative assessment of Hypnea musciformis (Wulfen) J.V. Lamouroux, Ochtodes secundiramea (Montagne) M.A. Howe, and Pterocladiella capillacea (S.G. Gmelin) Santelices & Hommersand (Rhodophyta) in order to determine the AChE effects from their extracts. As a matter of fact, the O. secundiramea extract showed 48% acetylcholinesterase inhibition at 400 μg/ml. The chemical composition of the bioactive fraction was determined by gas chromatography–mass spectrometry (GC–MS); this fraction is solely composed of halogenated monoterpenes, therefore allowing assignment of acetylcholinesterase inhibition activity to them.

Chemical analysis and toxicity of seaweed extracts with inhibitory activity against tropical fruit anthracnose fungi.

BACKGROUND: Banana and papaya are among the most important crops in the tropics, with a value amounting to millions of dollars per year. However, these fruits suffer significant losses due to anthracnose, a fungal disease. It is well known that certain seaweed extracts possess antifungal activity, but no published data appear to exist on the practical application of this property. In the present study, five organic Brazilian seaweed extracts were screened for their activity against banana and papaya anthracnose fungi. Furthermore, cytotoxic and mutagenic effects of the extracts were evaluated by the brine shrimp lethality assay and the Allium cepa root-tip mutagenicity test respectively, while their major components were identified by gas chromatography/mass spectrometry. RESULTS: Strong fungus-inhibitory effects of Ochtodes secundiramea and Laurencia dendroidea extracts were observed on both papaya (100 and 98% respectively) and banana (89 and 78% respectively). This impressive activity could be associated with halogenated terpenes, the major components of both extracts. Only Hypnea musciformis extract showed cytotoxic and mutagenic effects. CONCLUSION: The results of this study suggest the potential use of seaweed extracts as a source of antifungal agents with low toxicity to control anthracnose in papaya and banana during storage.