Navigating Antibacterial Frontiers: A Panoramic Exploration of Antibacterial Landscapes, Resistance Mechanisms, and Emerging Therapeutic Strategies.

The development of effective antibacterial solutions has become paramount in maintaining global health in this era of increasing bacterial threats and rampant antibiotic resistance. Traditional antibiotics have played a significant role in combating bacterial infections throughout history. However, the emergence of novel resistant strains necessitates constant innovation in antibacterial research. We have analyzed the data on antibacterials from the CAS Content Collection, the largest human-curated collection of published scientific knowledge, which has proven valuable for quantitative analysis of global scientific knowledge. Our analysis focuses on mining the CAS Content Collection data for recent publications (since 2012). This article aims to explore the intricate landscape of antibacterial research while reviewing the advancement from traditional antibiotics to novel and emerging antibacterial strategies. By delving into the resistance mechanisms, this paper highlights the need to find alternate strategies to address the growing concern.

Mining conditions specific hub genes from RNA-Seq gene-expression data via biclustering and their application to drug discovery.

Gene-expression data is being widely used for various clinical research. It represents expression levels of thousands of genes across the various experimental conditions simultaneously. Mining conditions specific hub genes from gene-expression data is a challenging task. Conditions specific hub genes signify the functional behaviour of bicluster across the subset of conditions and can act as prognostic or diagnostic markers of the diseases. In this study, the authors have introduced a new approach for identifying conditions specific hub genes from the RNA-Seq data using a biclustering algorithm. In the proposed approach, efficient 'runibic' biclustering algorithm, the concept of gene co-expression network and concept of protein-protein interaction network have been used for getting better performance. The result shows that the proposed approach extracts biologically significant conditions specific hub genes which play an important role in various biological processes and pathways. These conditions specific hub genes can be used as prognostic or diagnostic biomarkers. Conditions specific hub genes will be helpful to reduce the analysis time and increase the accuracy of further research. Also, they summarised application of the proposed approach to the drug discovery process.

COSCEB: Comprehensive search for column-coherent evolution biclusters and its application to hub gene identification.

Biclustering is an increasingly used data mining technique for searching groups of co-expressed genes across the subset of experimental conditions from the gene-expression data. The group of co-expressed genes is present in the form of various patterns called a bicluster. A bicluster provides significant insights related to the functionality of genes and plays an important role in various clinical applications such as drug discovery, biomarker discovery, gene network analysis, gene identification, disease diagnosis, pathway analysis etc. This paper presents a novel unsupervised approach 'COmprehensive Search for Column-Coherent Evolution Biclusters (COSCEB)' for a comprehensive search of biologically significant column-coherent evolution biclusters. The concept of column subspace extraction from each gene pair and Longest Common Contiguous Subsequence (LCCS) is employed to identify significant biclusters. The experiments have been performed on both synthetic as well as real datasets. The performance of COSCEB is evaluated with the help of key issues. The issues are comprehensive search, Deep OPSM bicluster, bicluster types, bicluster accuracy, bicluster size, noise, overlapping, output nature, computational complexity and biologically significant biclusters. The performance of COSCEB is compared with six all-time famous biclustering algorithms SAMBA, OPSM, xMotif, Bimax, Deep OPSM- and UniBic. The result shows that the proposed approach performs effectively on most of the issues and extracts all possible biologically significant column-coherent evolution biclusters which are far more than other biclustering algorithms. Along with the proposed approach, we have also presented the case study which shows the application of significant biclusters for hub gene identification.

Identifying condition specific key genes from basal-like breast cancer gene expression data.

Mining patterns of co-expressed genes across the subset of conditions help to narrow down the search space for the analysis of gene expression data. Identifying conditions specific key genes from the large-scale gene expression data is a challenging task. The conditions specific key gene signifies functional behavior of a group of co-expressed genes across the subset of conditions and can be act as biomarkers of the diseases. In this paper, we have propose a novel approach for identification of conditions specific key genes from Basal-Like Breast Cancer (BLBC) disease using biclustering algorithm and Gene Co-expression Network (GCN). The proposed approach is a two-stage approach. In the first stage, significant biclusters have been extracted with the help of 'runibic' biclustering algorithm. The second stage identifies conditions specific key genes from the extracted significant biclusters with the help of GCN. By using difference matrix and gene correlation matrix, we have constructed biologically meaningful and statistically strong GCN. Also, presented the proposed approach with the help of a process diagram and demonstrated the procedure with an example of bicluster number 93 (Bic93). From the experimental results, we observed that 95% and 85% of the extracted biclusters are found to be biologically significant at the p-values less than 0.05 and 0.01 respectively. We have compared proposed approach with the Weighted Gene Co-expression Network Analysis (WGCNA) based approach. From the comparison, our approach has performed effectively and extracted biologically significant biclusters. Also, identified conditions specific key genes which cannot be extracted using the WGCNA based approach. Some of the important identified known key genes are PIK3CA, SHC3, ERBB2, SHC4, PTOV1, STAG1, ZNF215 etc. These key genes can be used as a diagnostic and prognostic biomarker for the BLBC disease after the rigorous analysis. The identified conditions specific key genes can be helpful to reduce the analysis time and increase the accuracy of further research such as biomarker identification, drug target discovery etc.