Quality of Diet of Patients with Coeliac Disease in Comparison to Healthy Children.

A lifelong strict gluten-free diet is the only available treatment for patients with coeliac disease (CD). As with any restrictive diet, it may potentially lead to nutritional deficits. Seventy-six patients with CD (mean age 9.0 ± 4.3 years, 57% female) and 590 healthy controls (HC) (mean age 9.9 ± 0.1 years, 54% female) were recruited and requested to keep a 3-day food record (3DFR). In HC patients, anthropometric data were determined at the time when 3DFRs were collected. In CD patients, anthropometric data were determined at two time points: at diagnosis and at the time of 3DFRs collection. Intake of energy, macronutrients, and micronutrients was determined using PRODI expert 6.9 software and expressed as a percentage of recommended daily intake. In CD patients, all measured anthropometric measures (body weight (BW), body height (BH), and body mass index (BMI) z-scores) increased significantly after the mean duration of 34.1 months of a GFD. Overall, CD patients had significantly lower BW and BH z-scores compared to healthy controls. Patients with CD were generally more compliant with the recommended dietary intakes of macronutrients and some micronutrients, as compared to HC. Three participants were not compliant with the GFD; 42.1% of participants took oral nutritional supplements at the start of their GFD. Our study showed that patients with CD have better compliance with dietary recommendations compared to healthy controls, showing that a well-balanced GFD diet can provide necessary macro- and micronutrients.

Rapid quantification of dissolved solids and bioactives in dried root vegetable extracts using near infrared spectroscopy.

Artificial neural networks (ANN) were developed for prediction of total dissolved solids, polyphenol content and antioxidant capacity of root vegetables (celery, fennel, carrot, yellow carrot, purple carrot and parsley) extracts prepared from the (i) fresh vegetables, (ii) vegetables dried conventionally at 50 °C and 70 °C, and (iii) the lyophilised vegetables. Two types of solvents were used: organic solvents (acetone mixtures and methanol mixtures) and water. Near-infrared (NIR) spectra were recorded for all samples. Principal Component Analysis (PCA) of the pre-treated spectra using Savitzky-Golay smoothing showed specific grouping of samples in two clusters (1st: extracts prepared using methanol mixtures and water as the solvents; 2nd: extracts prepared using acetone mixtures as the solvents) for all four types of extracts. Furthermore, obtained results showed that the developed ANN models can reliably be used for prediction of total dissolved solids, polyphenol content and antioxidant capacity of dried root vegetable extracts in relation to the recorded NIR spectra.

In-vitro digestion of the bioactives originating from the Lamiaceae family herbal teas: A kinetic and PLS modeling study.

The stability of lavender, lemon balm, mint, sage, and thyme teas was investigated using in-vitro simulation of the digestive system. Kinetics of changes in the total polyphenolic content (TPC) and the antioxidant activity during the in-vitro trials were also evaluated. Results showed that TPC of mint teas decreases the fastest. Mathematical models for prediction of the TPC and the antioxidant activity of prepared teas based on UV-VIS and NIR spectra collected before, during, and after simulation showed that the best prediction was obtained for the wavelength ranges from 410 to 900 nm, 904 to 928 nm, and 1,399 to 1699 nm. It was concluded that the NIR can be used for calibration, validation, and classification of teas prepared from Lamiaceae plants. PRACTICAL APPLICATIONS: The bioactives' in-vitro digestion process can successfully be characterized by chemical, spectroscopic, and mathematical analysis. Application of NIR spectroscopy, in combination with multivariate analysis, leads to a reduction of time, costs, and chemical consumption and gives reliable results that pharmaceutical, food, and chemical industries can benefit from.