Inferência bayesiana da conversão alimentar em diferentes experimentos animais / Bayesian Inference of feed conversion in different animal experiments

O presente trabalho teve por objetivo avaliar a conversão alimentar (CA) por meio da inferência bayesiana considerando-se análises bivariadas. Foram utilizadas diferentes espécies animais de experimentos conduzidos na Universidade Federal de Viçosa, no estado de Minas Gerais, Brasil. O modelo proposto mostrou ser apropriado, uma vez que possibilitou a detecção de diferenças significativas entre níveis de fatores não detectados por procedimentos frequentistas em ANOVA tradicional, principalmente em pequenas amostras. No experimento com codornas, evidenciou-se que aves cujos níveis de proteína bruta eram de 23% e 29%, respectivamente, para machos e fêmeas, apresentaram uma melhor CA, de 2,83±0,03 e 2,66±0,03, respectivamente. No experimento com frangos, no grupo sem o aditivo antibiótico, a inclusão de 0,02% de extrato de ésteres naturais foi o que promoveu a melhor CA (1,72±0,01), e, de modo geral, o uso de antibiótico e a ausência de ésteres naturais promoveram CA de 1,63±0,02. Em caprinos, verificou-se que o aleitamento, seja com leite de cabra ou de vaca, promove igualmente uma melhor CA, respectivamente, no grupo de 60 e 90 dias, de 1,29±0,14 e 1,79±0,11, sugerindo que o aleitamento seja feito até os 60 dias. Em suínos, a dieta com maior nível de energia metabolizável e aminoácidos foi a que promoveu a melhor CA (2,86±0,07), quando comparada a uma dieta com nível nutricional mais baixo. Já o uso de enzimas na dieta com menor nível energético e de aminoácidos proporcionou resultado intermediário (2,90±007). Em bovinos, observou-se que o uso de 1% de concentrado na dieta promoveria uma melhor CA estimada de 7,33±0,35 entre os Nelores e que essa promoção seria de 7,40±0,58 entre os cruzados com o uso de 2% de concentrado na dieta.

The aim of this study was to evaluate the feed conversion (CA) by Bayesian inference in bivariate considering analyzes in real and simulated data. Different animal species experiments conducted at the Universidade Federal de Viçosa, state of Minas Gerais, Brazil are used. The proposed model proved to be appropriate once it enabled the detection of significant differences between levels of factors not detected by frequentist procedures with traditional ANOVA, especially in small samples. In the experiment with quails, it became clear that the birds' brute protein levels were 23% and 29%, respectively, for males and females, which presented better CA, 2.83±0.03 and 2.66±0.03, respectively. In the experiment with chickens, the group without additive antibiotic, including 0.02% extract natural esters promoted the best CA (1.72±0.01) and in general antibiotic absence of esters natural promoted 1.63±0.02 of the CA. In goats, it has been found that feeding milk from cows or goats also promotes better CA, respectively, in groups milked up to 60 and 90 days, being 1.29±0.14 and 1.79±0.11, suggesting that suckling done until 60 days. Pigs fed the highest level of metabolizable energy and aminoacids promoted the best CA (2.86±0.07) compared to a diet with lower nutritional level. But the use of enzymes in the diet with lower energy level and amino acid provided intermediate result (2.90±007). In cattle, it was observed that the use of 1% concentrate diet, CA, promotes a better estimate of 7.33±0.35 between Nellore and this promotion would be 7.40±0.58 between the cross breeds using 2% concentrate diet.

Glicerina bruta para codornas de corte, de um a 14 e de 15 a 35 dias de idade / Crude glycerin for meat quails, from 1 to 14 and 15 to 35 days old

Três experimentos foram conduzidos para determinar o valor nutritivo da glicerina bruta (GB), bem como verificar o desempenho de codornas de corte alimentadas com essa fonte alternativa de alimento. No experimento I, foram utilizadas 75 codornas, distribuídas em delineamento inteiramente ao acaso (DIC), com três tratamentos e cinco repetições. Os tratamentos consistiram de uma ração referência (RR) e duas rações teste (RT), e a GB substituiu a RR em níveis de oito e 12%. O valor estimado de energia metabolizável aparente corrigida para balanço de nitrogênio (EMAn) foi de 4.893kcal/kg. No experimento II, de um a 14 dias de idade, foram utilizadas 1.320 codornas, não sexadas, distribuídas em DIC, com seis tratamentos e quatro repetições. Os tratamentos foram constituídos por seis rações com níveis crescentes (0, 3, 6, 9, 12 e 15%) de GB. O peso vivo (PV), o ganho de peso (GP), o consumo de ração (CR), a conversão alimentar (CA), a biomassa corporal acumulada (BCA) e a umidade da cama (UC) aumentaram linearmente com a inclusão de GB. No experimento III, de 15 a 35 dias de idade, foram utilizadas 1.032 codornas, distribuídas sob as mesmas condições do experimento II. O GP, o CR, a BCA, a UC, o peso de peito (PPEI) e o rendimento de peito (RPEI) aumentaram linearmente com a inclusão de GB. Considerando-se os dados de desempenho, a GB pode ser incluída até o nível de 15%...

Three experiments were carried out aiming to determine the nutritive value and verify the performance of meat quails feed with crude glycerin (CG). In the first trial 75 quails were used, allocated in a completely randomized design, consisting in three treatments and five replications. The treatments consisted of one reference diet (RD) and two test diets (TD), in which the CG replaced the RD at levels of eight and 12%. The estimated value of metabolizable energy corrected for nitrogen balance was 4,893kcal/kg. In experiment two o, from d 1 to 14, 1,320 not sexed quails were used, allotted in a completely randomized design, with six treatments and 4 four replications. The treatments consisted of six diets with increased levels of CG (0; 3; 6; 9; 12 and 15%). The live weight (LW), weight gain (WG), feed intake (FI), feed conversion (FC), biomass accumulated body (BAB) and litter moisture (LM) increased linearly with the inclusion of CG. Considering the performance data, it can be concluded that CG can be included in the diets up to15%. In experiment three, d 15 to 35, 1,032 quails were used, allocated under the same conditions of experiment two. The WG, FI, BAB, LM, breast weight (BW) and breast yield (BY) increased linearly with the inclusion of CG. Considering the performance data, it can be concluded that the CG can be included in the diets up to the level of 15%...

Productive performance, eggshell quality, and eggshell ultrastructure of laying hens fed diets supplemented with organic trace minerals.

This study was carried out with the purpose of evaluating the effect of supplementing hens' diets with trace minerals from inorganic or organic sources on the productive performance, eggshell quality, and eggshell ultrastructure of laying hens. Three hundred sixty Hy-Line W36 laying hens between 47 to 62 wk of age were used and distributed in a completely randomized experimental design with 9 treatments, 5 replicates, and 8 birds for each experimental unit. The treatments consisted of a control diet without supplementation of the trace minerals Mn, Zn, and Cu; 4 supplementation levels of these trace minerals from an inorganic source; and the same levels of supplementation from an organic source (proteinates). The supplementation levels in milligrams per kilogram for Mn, Zn, and Cu, were, respectively, 35-30-05, 65-60-10, 95-90-15, and 125-120-20. There was no effect of supplementation of trace minerals on the rate of posture, feed intake, feed conversion, specific weight, and Haugh unit of eggs. However, there was a quadratic effect (P < 0.05) of the levels of trace mineral supplementation on average egg weight and egg mass; the results did not differ regarding the source used. The increase in the levels of supplementation of Mn, Zn, and Cu provided a linear increase (P < 0.05) in the breaking strength and the percentage of eggshell. There was a linear decrease (P < 0.05) in the egg loss and the number of mammillary buttons in the shell. The best results were obtained using diets supplemented with trace minerals from an organic source because these diets provided lower egg loss, higher thickness, and increased strength of the shell. Structurally, organic Mn, Zn, and Cu provided higher thickness of the palisade layer and lower mammillary density. The trace mineral supplementation improved the structural characteristics and the quality of the eggshells.

Exigência de treonina digestível para codornas de corte no período de 15 a 35 dias de idade / Digestible threonine requirements for quail meat from 15 to 35 days of age

Estimou-se a exigência de treonina digestível (ThrD) para codornas de corte (Coturnix coturnix sp.) em fase de crescimento no período de 15 a 35 dias de idade. Foram utilizadas 1020 codornas com 15 dias de idade, não sexadas, distribuídas em um delineamento experimental inteiramente ao acaso, com seis porcentagens de adição de treonina digestível - 0,93; 1,00; 1,07; 1,14; 1,21 e 1,28% na dieta - , cinco repetições e 34 codornas por unidade experimental. Não houve efeito sobre o desempenho, o rendimento e a composição química da carcaça e sobre o balanço de nitrogênio com o aumento da porcentagem de treonina digestível na dieta. Houve aumento linear no consumo de treonina e no custo da ração por quilograma de peso vivo ganho com o aumento da porcentagem de treonina digestível. Concluiu-se que o melhor nível de treonina digestível avaliado foi suficiente para atender as exigências de codornas de corte, no período de 15 a 35 dias de idade.

The digestible threonine (DThr) requirements for meat quails (Coturnix coturnix sp) during growing phase from 15 to 35 days of age were estimated, using 1,020 quails with 15 days of age, of both sexes, distributed in a completely randomized design, with six levels of digestible threonine - 0.93; 1.00; 1.07; 1.14; 1.21 and 1.28% in diet, five replications and 34 quails per experimental unit. There was no effect on performance variables, carcass yield and chemical composition and nitrogen balance with the studied increase in digestible threonine levels. There was a linear increase in threonine intake andfeed cost per kilogram of body weight gain with increasing levels of digestible threonine. It was concluded that the best level of threonine evaluated was sufficient to meet the requirements of meat quails, in the period from 15 to 35.

The effect of glycerol on mRNA expression of growth hormone, insulin-like growth factor, and mitochondrial breast muscle genes of Japanese quail.

1. A study was carried out to evaluate the expression of growth hormone (GH), insulin-like growth factor I (IGF-I), mitochondrial adenine nucleotide translocase (ANT), cytochrome oxidase III (COX III) and avian uncoupling protein (avUCP) genes in 14-d-old Japanese quail that were fed different levels of glycerol (0%, 4%, and 12% dietary glycerol) which replaced maize as an energy source. 2. Total RNA was extracted from the breast muscle, and cDNA was amplified using real-time PCR with primers specific to the examined genes. 3. Quail fed the diet with 12% glycerol supplementation presented higher growth hormone (GH) mRNA expression than did those fed 0% glycerol. Supplementation with 12% glycerol negatively influenced IGF-I mRNA expression and reduced ANT mRNA expression in comparison with the treatment with no glycerol. COX III mRNA expression in the pectoralis superficialis muscle was decreased by 26% in quail fed 12% glycerol compared with those fed 0 and 4% glycerol. 4. There was no difference in UCP mRNA expression between quail fed 0 and 4% glycerol; however, UCP expression was reduced (73%) in birds fed the 12% glycerol diet compared with the level in those fed the 4% glycerol diet. 5. The inclusion of 4% glycerol in the diet produced results similar to those of the diet with no glycerol. Based on quail performance and the expression of the GH, IGF-I, ANT, COX III and UCP genes, 4% glycerol can be used in quail feeding without any harmful effects.

Effect of arginine on the development of the pectoralis muscle and the diameter and the protein:deoxyribonucleic acid rate of its skeletal myofibers in broilers.

This work aimed at evaluating the effects of the supplementation of starter diet with Arg on breast muscle development in broilers and the activation of satellite cells and the aggregation of myofibrillar protein. Male Cobb chicks (n = 990) were randomly assigned to 1 of 5 treatments in a complete random design. Measurements of 33 chicks per treatment were made in 6 repetitions. The treatments consisted of a basal diet with 1.390% digestible Arg (without supplementation) and 4 dietary levels of Arg (1.490, 1.590, 1.690, and 1.790%) with Arg:Lys ratios of 1.103, 1.183, 1.262, 1.341, and 1.421, respectively. Arginine supplementation was used only in the starter phase (1 to 21 d). Dietary supplementation with Arg had a positive effect (P < 0.05) on breast and breast fillet weight on d 7 and 21 and on myofiber diameter on d 14 and 21. However, no effect was observed (P > 0.05) on the protein:DNA ratio, which demonstrates that Arg does not interfere with the mitotic activity of the satellite cells. Independently from mechanism, Arg affected muscle growth in the starter phase positively. Dietary supplementation with Arg in the starter phase had no effect (P > 0.05) on the carcass yield of broilers on d 42. Diet supplementation with Arg at levels above the ones recommended for the starter phase may be necessary for improved muscle development in broilers.

Evaluation of internet websites about retinopathy of prematurity patient education.

BACKGROUND/AIMS: The success of the treatment in patients with retinopathy of prematurity (ROP) is mainly associated with timely diagnosis and appropriate management. Information dissemination is crucial for the outcome of ROP. This study aimed to evaluate the quality of the information about ROP available for patients on the internet. METHODS: Cross sectional study. In March 2004 the ROP information available on the internet was evaluated using two search engines (MetaCrawler and MSN) and four key terms ("retinopathy of prematurity," "premature eye," "premature retina," and "ROP"). The quality of each website was evaluated using a score system. The sites were classified as academic, organisational, or commercial. Readability, general quality of the website (based on ownership, purpose, authorship, author qualification, attribution, interactivity, and currency), and quality of the content specific to ROP (definition, causes, epidemiology, risk factors, diagnosis, classification, treatment, and prognosis) were analysed. RESULTS: Of 114 websites evaluated, 40 were included. 10 sites (25.0%) were academic, eight (20.0%) organisational, and 22 (55.0%) commercial. In the majority of the sites (62.5%) the ROP information was fair or poor. CONCLUSIONS: A large amount of information about ROP is available on the internet. However, most websites were considered incomplete.

Aerobic bacterial conjunctival flora in diabetic patients.

OBJECTIVE: To study the aerobic conjunctival flora of diabetic patients and its relation to the presence and level of diabetic retinopathy and the duration of the disease. METHODS: One hundred three patients from the diabetic retinopathy screening program of the Federal University of São Paulo with no evidence of ocular surface disease were included. The diabetic patient cohort was compared with 60 nondiabetic subjects. All patients underwent slit-lamp evaluation, conjunctival scrapings, and indirect ophthalmoscopy. RESULTS: The frequency of positive conjunctival cultures was significantly higher in the diabetic group (94.18%) than in the nondiabetic group (73.33%). Among diabetic patients, a significantly higher frequency of positive cultures was detected in those with diabetic retinopathy than in those without retinopathy. Neither the duration of the diabetes nor the hypoglycemic therapy correlated with the culture results. Coagulase-negative Staphylococcus was the most common microorganism isolated, and its identification was more frequent in patients with retinopathy than in those without diabetic retinopathy. CONCLUSION: Diabetic patients have a significantly higher number of positive conjunctival cultures. The presence of diabetic retinopathy was correlated with an increase in positive cultures and a higher proportion of coagulase-negative Staphylococcus.

Trajetória de crescimento e efeito da idade da vaca nos modelos de regressäo aleatória de bovinos jovens da raça Tabapuä / Growth trajectory and age-of-dam effect for random regression models of young Tabapuä beef cattle

Para estabelecer a melhor forma de considerar os efeitos fixos dos modelos de regressäo aleatória, avaliou-se a utilizaçäo de funçöes polinomiais na descriçäo de curvas de crescimento e no efeito da idade da vaca sobre pesos corporais de 41.415 bovinos jovens da raça Tabapuä, criados em regime de pasto. A idade da vaca ao parto e o sexo do animal influenciaram os pesos nos primeiros dois anos de vida, e o efeito da idade da mäe sobre o desenvolvimento dos animais mostrou-se dependente da idade dos filhos. Altos coeficientes de determinaçäo (R²>0,98) foram alcançados utilizando-se o efeito da idade da vaca no dia da pesagem do animal (i.e., a idade da vaca ao parto mais a idade do animal no dia de sua pesagem) em polinômios de, no mínimo, segundo grau, e curvas de crescimentos médios, diferenciadas para machos e fêmeas, descritas por meio de polinômios de, no mínimo, terceiro grau

Sodium and chloride requirements of growing broiler chickens (twenty-one to forty-two days of age) fed corn-soybean diets.

Two trials were conducted to determine Na+ and Cl- nutritional requirements and dietary electrolyte balance (DEB) and its effects on acid-base balance, litter moisture, and incidence of tibial dyschondroplasia (TD) in broiler chickens during the growing period. Cobb broilers were distributed in a completely randomized design (30 pens) with six treatments, five replicates, and 50 birds per experimental unit at 21 d of age. Treatments used in both trials were a basal diet with 0.10% Na+ (Trial 1) or Cl- (Trial 2) supplemented to result in diets with Na+ or Cl- levels of 0.10, 0.15, 0.20, 0.25, 0.30, and 0.35%. In the first trial, the results indicated an optimum Na+ requirement of 0.15%. The Na+ levels, obtained with supplemental NaHCO3, did not affect blood gas parameters and TD incidence. Litter moisture increased linearly with Na+ levels. In the second trial, the Cl- requirement was estimated at 0.23%. Increasing Cl- levels, provided by NaCl with NaHCO3 to balance Na+, caused a linear effect (P < or = 0.01) on blood gas parameters, with an estimated equilibrium at 0.19% dietary Cl-. No effect (P > or = 0.05) of Cl- levels on litter moisture was observed. The hypertrophic area of growth plate in the proximal tibiotarsus increased with Cl- levels (P < or = 0.001). A nonlinear model describes this response. The best dietary electrolyte balance (DEB) was between 250 to 261 mEq/kg in Trial 1 and 249 to 257 mEq/kg in Trial 2. We concluded that the Na+ requirement was 0.15%, and the Cl- requirement was 0.23% for maximum performance of growing chickens between 21 and 42 d of age, and the best DEB was between 249 and 261 mEq/kg.

Desempenho de cordeiros Corriedale, puros e mestiços, terminados em pastagem e em confinamento / Performance of Corriedale and crossbred lambs raised on pasture and on drylot

O experimento teve por objetivo avaliar o desempenho de cordeiros, do nascimento ao abate com 30-32kg de peso vivo na origem, usando-se 23 cordeiros Corriedale (C), 25 Bergamácia x Corriedale (BC) e 17 Hampshire Down x Corriedale (HC), terminados em pastagem de coast cross (Cynodon dactylon) ou em confinamento. O peso ao nascimento e o ganho de peso médio diário do nascimento ao desmame näo foram influenciados pelo grupo genético (P>0,05). O ganho de peso médio diário do desmame ao abate (GPDA) de 0,144kg dos cordeiros confinados foi superior aos 0,106kg dos cordeiros em pastagem (P<0,05). O GPDA dos cordeiros BC (0,136kg) e HC (0,130kg) foram maiores (P<0,05) que o dos C (0,106kg). O sistema de terminaçäo e de cruzamento (P<0,05) influiu no ganho de peso do nascimento ao abate (GPNA), com maiores ganhos observados para cordeiros confinados (0,137kg) e cruzados (0,132kg). Os cordeiros confinados atingiram o peso de abate (219 dias) mais cedo (P<0,05) que os da pastagem (258 dias). Os cordeiros terminados em confinamento, no dia do abate, apresentaram menor perda de peso da origem ao abate (3,16 por cento), comparados aos da pastagem (5,17 por cento) (P<0,05). Os resultados indicam que pode-se recomendar o cruzamento de ovelhas Corriedale com carneiros das raças citadas, bem como a terminaçäo dos cordeiros em confinamento, desde que se tenha preocupaçäo de avaliar os custos de produçäo deste sistema