The effects of radial oxygen loss on arsenic tolerance and uptake in rice and on its rhizosphere.

Understanding the complex biotic and abiotic interactions invoked by the rice root system in oxygen-depleted soil is an important step in screening genotypes for low toxic metal or metalloid accumulation. A hydroponic and a rhizobox experiment have been conducted to explore the effects of varying root oxygen release on chemical changes, As fractionation in rhizosphere soil and Fe plaque formation, As uptake and tolerance by different rice genotypes. The results showed that rice genotypes with higher rates of radial oxygen loss (ROL) and at the bolting stage, tended to have greater effects on rhizosphere Eh, pH, Fe(3+)/Fe(2+) quotients, As fractionation and mobility and also on Fe plaque formation compared to those with lower ROL and at the tillering stage. Genotypes with higher ROL have a strong ability to reduce As accumulation in shoots and increase As tolerance by reducing As mobilization in the rhizosphere and by limiting As translocation.

The relationship of root porosity and radial oxygen loss on arsenic tolerance and uptake in rice grains and straw.

The correlations among arsenic (As) accumulation in grains and straw, rates of radial oxygen loss (ROL), and porosity of roots using 25 rice cultivars were investigated based on two pot experiments: (1) soil with addition of 100mg As kg(-1) for analysis of As in grains and straw, and (2) deoxygenated solution for analyzing rates of ROL and porosity of roots. The results showed that there were great differences in grain As (0.71-1.72 mg kg(-1)) and straw As (15.6-31.7 mg kg(-1)), rates of ROL (7.40-13.24 mmol O(2)kg(-1)root d.w. h(-1)), and porosity (20.91-33.08%) among the cultivars. There were significant negative correlations between As in grains or straw and ROL and porosity, and significant positive correlations between rates of ROL and porosities, respectively. Rice cultivars with high porosities tended to possess higher rates of ROL, and had higher capacities for limiting the transfer of As to aboveground tissues.