Raw milk cheeses from Beira Baixa, Portugal-A contributive study for the microbiological hygiene and safety assessment.

Due to specific bacterial microbiota, raw milk cheeses have appreciated sensory properties. However, they may pose a threat to consumer safety due to potential pathogens presence. This study evaluated the microbiological contamination of 98 raw milk cheeses from Beira Baixa, Portugal. Presence and enumeration of Coagulase Positive Staphylococci (CPS), Listeria monocytogenes, Salmonella spp., pathogenic Escherichia coli, and indicator microorganisms (non-pathogenic E. coli and Listeria spp.) was attained. E. coli antimicrobial resistance (AMR) was also evaluated. PCR and/or Whole genome sequencing (WGS) was used to characterize E. coli, Salmonella spp. and L. monocytogenes isolates. Sixteen cheeses (16.3%) were classified as Satisfactory, 59 (60.2%) as Borderline and 23 (23.5%) as Unsatisfactory/Potential Injurious to Health. L. monocytogenes, CPS > 104 cfu g-1, Extraintestinal pathogenic E. coli (ExPEC) and Salmonella spp. were detected in 4.1%, 6.1%, 3.1% and 1.0% of the samples, respectively. Listeria innocua (4.1%) and E. coli > 104 cfu g-1 (16.3%) were also detected. AMR E. coli was detected in 23/98 (23.5%) of the cheese samples, of which two were multidrug resistant. WGS identified genotypes already associated to human disease and Listeria spp. cluster analysis indicated that cheese contamination might be related with noncompliance with Good Hygiene Practices during cheese production.

Two Cultural Processing Asymmetries Drive Spatial Attention.

Cultural routines, such as reading and writing direction (script direction), channel attention orientation. Depending on one's native language habit, attention is biased from left-to-right (LR) or from right-to-left (RL). Here, we further document this bias, as it interacts with the spatial directionality that grounds time concepts. We used a spatial cueing task to test whether script direction and the grounding of time in Portuguese (LR, Exp. 1) and Arabic (RL, Exp. 2) shape visuomotor performance in target discrimination. Temporal words (e.g., tomorrow, yesterday) were presented as cues in two modalities: visual (Exp. 1-2) and auditory (Exp. 1). Gaze movement (Exp. 1) and speed of discrimination decisions (Exp. 1-2) of targets presented to the left or right sides of the screen were assessed. As predicted, the interaction between target location and time concepts was significant across both modalities and linguistic communities. Additionally, LR participants detected the target on the right side of the screen faster after a future word than the target on the left side of the screen after a past word cue. In contrast, RL participants detected the target on the left side of the screen faster when the cue word was a future word than the target on the right side of the screen cued by a past word. In both modalities, the initial eye-gaze movement (Exp. 1) was responsive to the cue's time referent, further confirming that time orients attention. An additional bias was observed for the first fixation onset, which landed earlier on the target set that matched habitualized spatial routines. We conclude that scanning regularities are shaped by writing habits and bodily grounded categorical features.

Taxa de morbimortalidade entre homens e mulheres com diagnóstico de infarto agudo do miocárdio / Morbidity and mortality rate among men and women diagnosed with myocardial infarction / Tasa de morbimortalidad entre hombres y mujeres diagnosticados con infarto agudo del miocardio

Objetivo: verificar se existe diferença na taxa de morbimortalidade entre homens e mulheres com diagnóstico de infarto agudo do miocárdio em um hospital de ensino. Método: estudo transversal, descritivo e quantitativo, com amostra de 647 prontuários, comparando-se fatores de risco, dados sociodemográficos e referentes à internação hospitalar. Resultados: de 647 pacientes, 415 eram homens e 232 mulheres, com idade variando entre 61 e 80 anos. Dos fatores de risco, 74,96% eram hipertensos em ambos os sexos, sendo que o percentual de hipertensão, diabetes e dislipidemia foi maior no sexo feminino. A taxa de mortalidade foi de 17,24% nas mulheres e 9,64% nos homens. Dentre os fatores de risco relacionados ao óbito, o mais significante foi a hipertensão arterial, seguido de diabetes e dislipidemia. Conclusão: houve maior prevalência de infarto do miocárdio no sexo masculino, entretanto, a taxa de morbidade e mortalidade foi maior no sexo feminino.

Objective: to identify any difference in morbidity and mortality rate between men and women diagnosed with acute myocardial infarction. Method: this quantitative, descriptive, cross-sectional study, involving a sample of 647 medical records, compared risk factors, and sociodemographic and hospitalization-related data. Results: of 647 patients, 415 were men and 232 women aged from 61 to 80 years. Risk factors included 74.96% of both sexes were hypertensives. Percentages of hypertension, diabetes, and dyslipidemia were higher in women. Mortality rates were 17.24% in women and 9.64% in men. The most significant risk factors for mortality were arterial hypertension, followed by diabetes and dyslipidemia. Conclusion: prevalence of myocardial infarction was higher in men, but morbidity and mortality rates were higher in women.

Objetivo: : verificar si existe alguna diferencia en la tasa de morbimortalidad entre hombres y mujeres diagnosticados con infarto agudo de miocardio en un hospital universitario. Método: se trata de un estudio transversal, descriptivo y cuantitativo, que involucró una muestra de 647 historias clínicas, comparando factores de riesgo, datos sociodemográficos y relacionados con la hospitalización. Resultados: de 647 pacientes, 415 eran hombres y 232 mujeres con edades comprendidas entre 61 y 80 años. En cuanto a los factores de riesgo, el 74,96% era hipertenso en ambos sexos. El porcentaje de hipertensión, diabetes y dislipidemia se mostró superior entre las mujeres. La tasa de mortalidad fue del 17,24% en mujeres y del 9,64% en hombres. Entre los factores de riesgo relacionados con la muerte, el más significativo fue la hipertensión arterial, seguido de la diabetes y la dislipidemia. Conclusión: hubo una mayor prevalencia de infarto de miocardio entre hombres, sin embargo, la tasa de morbilidad y mortalidad fue más alta entre mujeres.

The Effect of Simultaneously Presented Words and Auditory Tones on Visuomotor Performance.

The experiment reported here used a variation of the spatial cueing task to examine the effects of unimodal and bimodal attention-orienting primes on target identification latencies and eye gaze movements. The primes were a nonspatial auditory tone and words known to drive attention consistent with the dominant writing and reading direction, as well as introducing a semantic, temporal bias (past-future) on the horizontal dimension. As expected, past-related (visual) word primes gave rise to shorter response latencies on the left hemifield and future-related words on the right. This congruency effect was differentiated by an asymmetric performance on the right space following future words and driven by the left-to-right trajectory of scanning habits that facilitated search times and eye gaze movements to lateralized targets. Auditory tone prime alone acted as an alarm signal, boosting visual search and reducing response latencies. Bimodal priming, i.e., temporal visual words paired with the auditory tone, impaired performance by delaying visual attention and response times relative to the unimodal visual word condition. We conclude that bimodal primes were no more effective in capturing participants' spatial attention than the unimodal auditory and visual primes. Their contribution to the literature on multisensory integration is discussed.

Asymmetric practices of reading and writing shape visuospatial attention and discrimination.

Movement is generally conceived of as unfolding laterally in the writing direction that one is socialized into. In 'Western' languages, this is a left-to-right bias contributing to an imbalance in how attention is distributed across space. We propose that the rightward attentional bias exercises an additional unidirectional influence on discrimination performance thus shaping the congruency effect typically observed in Posner-inspired cueing tasks. In two studies, we test whether faces averted laterally serve as attention orienting cues and generate differences in both target discrimination latencies and gaze movements across left and right hemifields. Results systematically show that right-facing faces (i.e. aligned with the script direction) give rise to an advantage for cue-target pairs pertaining to the right (versus left) side of space. We report an asymmetry between congruent conditions in the form of right-sided facilitation for: (a) response time in discrimination decisions (experiment 1-2) and (b) eye-gaze movements, namely earlier onset to first fixation in the respective region of interest (experiment 2). Left and front facing cues generated virtually equal exploration patterns, confirming that the latter did not prime any directionality. These findings demonstrate that visuospatial attention and consequent discrimination are highly dependent on the asymmetric practices of reading and writing.

Murumuru (Astrocaryum murumuru) meal as an additive to elephant grass silage / Torta de murumuru (Astrocaryum murumuru) como aditivo en el ensilado de pasto elefante / Torta de murumuru (Astrocaryum murumuru) como aditivo em silagens de capim elefante

Abstract Background: Tropical grasses, such as elephant grass, have high moisture content during its ideal phenological state for silage. High moisture content hinders proper preservation and reduces the nutritive value of silage due to secondary fermentation and production of effluents. Adding feed materials with high dry matter content, such as murumuru (Astrocaryum murumuru) meal, is a potential alternative to improve silage yield. Objective: To determine the effects of including murumuru meal (0, 7, 14, 21, and 28%) on the fermentative characteristics, microbiological activity, aerobic stability, and chemical composition of elephant grass silages. Methods: A completely randomized design with five treatments and five replicates was used. Elephant grass was collected at 60 d of age, minced, and homogenized with murumuru meal. The mass was placed in experimental 15-L silos. The silos were collected and analyzed 45 d later. Results: Effluent production decreased (p<0.05) as the proportions of murumuru meal in silage increased. A quadratic effect (p<0.05) was observed on dry matter recovery. An increase (p<0.05) was observed in dry matter content, a decrease (p<0.05) in the neutral detergent fiber content, and an increase (p<0.05) in the non-fibrous carbohydrate content with the inclusion of murumuru meal. Conclusions: Addition of murumuru meal improves chemical composition and does not affect the fermentative characteristics of elephant grass silage, while it reduces effluent losses. Nevertheless, the inclusion of murumuru meal in the elephant grass silage decreased the time of aerobic stability.

Resumen Antecedentes: los pastos tropicales, tales como el pasto elefante, tienen alto contenido de humedad cuando están en su estado fenológico ideal para ensilar. Esto dificulta su adecuada preservación en el silo, reduciendo el valor nutritivo del ensilaje debido a fermentaciones secundarias y generación de efluentes. Una posible solución sería incluir materiales con alto contenido de materia seca, tales como la torta de murumuru (Astrocaryum murumuru). Objetivo: determinar el efecto de la inclusión de torta de murumuru (0; 7; 14; 21 y 28%) sobre las características fermentativas, microbiológicas, estabilidad aeróbica y composición química de los ensilajes de pasto elefante. Métodos: se utilizó un diseño completamente al azar, con cinco tratamientos y cinco repeticiones. El pasto elefante fue colectado a los 60 días de edad, luego picado y homogeneizado con la torta de murumuru. La masa fue colocada en silos experimentales con capacidad de 15 L. Luego de 45 días de ensilado, los silos fueron abiertos y las muestras fueron colectadas para su posterior análisis. Resultados: hubo reducción (p<0,05) en la producción de efluentes a medida que se incrementó la proporción de torta de murumuru en el ensilado. Se observó un efecto cuadrático (p<0,05) en la recuperación de materia seca. Hubo aumento (p<0,05) en los contenidos de materia seca con la adición de torta de murumuru. Se observó disminución (p<0,05) en el contenido de fibra detergente neutra y aumento (p<0,05) de carbohidratos no fibrosos. Conclusión: La adición de torta de murumuru mejora la composición química, reduce las perdidas por efluentes y no afecta las características fermentativas de ensilado de pasto elefante. Sin embargo, la inclusión de la torta de murumuru en el ensilado de pasto elefante disminuye el tiempo en estabilidad aeróbica.

Resumo Antecedentes: capins tropicais, como por exemplo o capim-elefante apresentam alto teor de umidade quando possuem produtividade compatível e estão no estádio fenológico adequado para ensilagem. Isso dificulta a adequada preservação no silo, ocasionando a redução do valor nutritivo da silagem devido a fermentações secundárias e a produção de efluentes. Uma possível solução seria o uso de aditivos com alto teor de matéria seca, como bolo de murumuru (Astrocaryum murumuru). Objetivo: determinar o efeito da inclusão da torta de murumuru (0; 7; 14; 21 e 28%) na ensilagem de capim-elefante sobre as características fermentativas, microbiológicas, estabilidade aeróbia e a composição química das silagens. Métodos: utilizou- se um delineamento inteiramente casualizado, com cinco tratamentos e cinco repetições. O capim-elefante foi colhido aos 60 dias de idade, o mesmo foi picado e homogeneizado à torta de murumuru. A massa foi ensilada em silos experimentais com capacidade de 15 L. Após 45 dias de ensilagem, os silos foram abertos e amostras foram coletadas para posteriores análises laboratoriais. Resultados: houve redução (p<0,05) na produção de efluentes à medida que se elevaram as proporções da torta de murumuru na ensilagem. Observou-se efeito quadrático (p<0,05) na recuperação de matéria seca. Houve aumento (p<0,05) nos teores de matéria seca com a adição da torta murumuru. Observou-se diminuição (p<0,05) nos teores de fibra em detergente neutro e aumento (p<0,05) nos teores de carboidratos não fibrosos. Conclusão: torta de murumuru como aditivo melhora a composição química, reduz perdas principalmente por efluente e não afeta as características fermentativas de silagens de capim- elefante. Entretanto, a inclusão da torta de murumuru na ensilagem de capim-elefante diminui o tempo em estabilidade aeróbia.

Social Inferences From Faces as a Function of the Left-to-Right Movement Continuum.

We examined whether reading and writing habits known to drive agency perception also shape the attribution of other agency-related traits, particularly for faces oriented congruently with script direction (i.e., left-to-right). Participants rated front-oriented, left-oriented and right-oriented faces on 14 dimensions. These ratings were first reduced to two dimensions, which were further confirmed with a new sample: power and social-warmth. Both dimensions were systematically affected by head orientation. Right-oriented faces generated a stronger endorsement of the power dimension (e.g., agency, dominance), and, to a lesser extent, of the social-warmth dimension, relative to the left and frontal-oriented faces. A further interaction between the head orientation of the faces and their gender revealed that front-facing females, relative to front-facing males, were attributed higher social-warmth scores, or communal traits (e.g., valence, warmth). These results carry implications for the representation of people in space particularly in marketing and political contexts. Face stimuli and respective norming data are available at www.osf.io/v5jpd.

Effects of inclusion of patauá, Oenocarpus bataua meal on elephant grass silage

Elephant grass silages have high moisture content, causing nutrient loss, mainly by effluent production. The use of moisture absorbent additives, such as patauá, Oenocarpus bataua meal, can be a strategy to reduce losses in these silages. The aim of this study was to determine the fermentation losses, microbiological composition, fermentative characteristics, and chemical composition of elephant grass silage with increasing levels of inclusion of patauá meal. The experiment was conducted in a completely randomized design, with four concentrations of patauá meal (7%, 14%, 21%, and 28%, based on fresh matter) and a control without patauá, and five replicates. The elephant grass was collected manually after 60 days of growth. After chopping, the grass was homogenized, mixed with the patauá meal according to the established treatments, and ensiled in experimental silos of 15 L. The inclusion of patauá meal in elephant grass silage had positive effects on the chemical composition and effluent loss. It significantly increased dry matter, organic matter, crude protein, nitrogen insoluble in neutral detergent and acid, ethereal extract, lignin, and non-fibrous carbohydrates of the silages. It also reduced effluent losses, neutral and acid detergent fiber contents, and cellulose. Our findings contribute towards reducing the environmental impact caused by the production of effluents during ensilage of tropical grasses and providing a potential use for the residue from the production of patauá oil, thus avoiding disposal in the environment. (AU)

Presence of respiratory viruses in equines in Brazil.

Equines are susceptible to respiratory viruses such as influenza and parainfluenza. Respiratory diseases have adversely impacted economies all over the world. This study was intended to determine the presence of influenza and parainfluenza viruses in unvaccinated horses from some regions of the state of São Paulo, Brazil. Blood serum collected from 72 equines of different towns in this state was tested by hemagglutination inhibition test to detect antibodies for both viruses using the corresponding antigens. About 98.6% (71) and 97.2% (70) of the equines responded with antibody protective titers (≥ 80 HIU/25µL) H7N7 and H3N8 subtypes of influenza A viruses, respectively. All horses (72) also responded with protective titers (≥ 80) HIU/25µL against the parainfluenza virus. The difference between mean antibody titers to H7N7 and H3N8 subtypes of influenza A viruses was not statistically significant (p > 0.05). The mean titers for influenza and parainfluenza viruses, on the other hand, showed a statistically significant difference (p < 0.001). These results indicate a better antibody response from equines to parainfluenza 3 virus than to the equine influenza viruses. No statistically significant differences in the responses against H7N7 and H3N8 subtypes of influenza A and parainfluenza 3 viruses were observed according to the gender (female, male) or the age (≤ 2 to 20 years-old) groups. This study provides evidence of the concomitant presence of two subtypes of the equine influenza A (H7N7 and H3N8) viruses and the parainfluenza 3 virus in equines in Brazil. Thus, it is advisable to vaccinate equines against these respiratory viruses.

Presence of resiiratory viruses in equines in Brazil / Presença de vírus respiratórios em equinos do Brasil

Equines are susceptible to respiratory viruses such as influenza and parainfluenza. Respiratory diseases have adversely impacted economies all over the world. This study was intended to determine the presence of influenza and parainfluenza viruses in unvaccinated horses from some regions of the state of São Paulo, Brazil. Blood serum collected from 72 equines of different towns in this state was tested by hemagglutination inhibition test to detect antibodies for both viruses using the corresponding antigens. About 98.6% (71) and 97.2% (70) of the equines responded with antibody protective titers (≥ 80 HIU/25µL) H7N7 and H3N8 subtypes of influenza A viruses, respectively. All horses (72) also responded with protective titers (≥ 80) HIU/25µL against the parainfluenza virus. The difference between mean antibody titers to H7N7 and H3N8 subtypes of influenza A viruses was not statistically significant (p > 0.05). The mean titers for influenza and parainfluenza viruses, on the other hand, showed a statistically significant difference (p < 0.001). These results indicate a better antibody response from equines to parainfluenza 3 virus than to the equine influenza viruses. No statistically significant differences in the responses against H7N7 and H3N8 subtypes of influenza A and parainfluenza 3 viruses were observed according to the gender (female, male) or the age (≤ 2 to 20 years-old) groups. This study provides evidence of the concomitant presence of two subtypes of the equine influenza A (H7N7 and H3N8) viruses and the parainfluenza 3 virus in equines in Brazil. Thus, it is advisable to vaccinate equines against these respiratory viruses.

Os equinos são susceptíveis aos vírus respiratórios, como o vírus influenza, e também tem sido citado o vírus parainfluenza. Doenças respiratórias têm impactado a economia em todo mundo. Este estudo intencionou determinar a presença dos vírus influenza e parainfluenza em equinos não vacinados de certas regiões do Estado de São Paulo, Brasil. Os soros coletados de 72 equinos, de diferentes cidades deste Estado, foram submetidos ao teste de Inibição da Hemaglutinação (IH) com objetivo de detectar anticorpos contra os referidos vírus, usando antígenos correspondentes. Cerca de 98,8% (72) e 97,2% (70) desses equinos responderam com títulos protetores (≥ 80 UIH/25µL) para os subtipos H7N7 e H3N8 de vírus influenza, respectivamente. Todos equinos (72) responderam com títulos protetores (≥ 80 UIH/25µL) contra o vírus parainfluenza 3. A diferença entre as médias de anticorpos contra o vírus influenza A não foi estatisticamente significante (p > 0,05). As médias de títulos dos vírus influenza e parainfluenza, por outro lado, demonstraram diferença estatisticamente significante (p < 0,001). Esses resultados indicam melhor resposta de anticorpos pelos equinos ao vírus parainfluenza 3 do que ao vírus da influenza equina. Nenhuma diferença estatística foi observada nas respostas contra os vírus da influenza equina A (H7N7 e H3N8) e parainfluenza 3, com relação ao gênero (fêmeas e machos) e grupo etário (≤ 2 até 20 anos) nos equinos avaliados. Este estudo fornece evidência da presença concomitante dos dois subtipos vírus influenza A (H7N7 e H3N8) e do parainfluenza 3 em cavalos no Brasil. Portanto, é aconselhável a vacinação dos cavalos contra esses vírus respiratórios.

Influenza viruses in adult dogs raised in rural and urban areas in the state of São Paulo, Brazil / Incidência do vírus influenza em cães adultos criados em áreas rural e urbana do estado de São Paulo, Brasil

In 1970, searching for the interspecies transmission of influenza viruses led to the first study on influenza viruses in domestic animals. Birds and mammals, including human beings, are their natural hosts; however, other animals may also play a role in the virus epidemiology. The objective was to investigate the incidence of influenza viruses in adult dogs raised in rural (9, 19.56%) and urban (37, 80.43%) areas in the state of São Paulo, Brazil. Dog serum samples were examined for antibodies to influenza viruses by the hemagglutination inhibition (HI) test using the corresponding antigens from the circulating viruses in Brazil. Dogs from rural areas presented antibodies to influenza A H3N2, and influenza A H7N7 and H3N8. In rural areas, dog sera displayed mean titers as 94.37, 227.88, 168.14, 189.62 HIU/25 µL for subtypes H1N1, H3N2, H7N7, H3N8, respectively. About 84% and 92% of dogs from urban areas exhibited antibodies to human influenza A H1N1 and H3N2, respectively, with statistical difference at p < 0.05 between the mean titers of antibodies to H1N1 and H3N2. About 92% and 100% were positive for H7N7 and H3N8, respectively. In dogs from urban areas, the mean titers of antibodies against influenza A H1N1, H3N2, H7N7 and H3N8, were 213.96, 179.42, 231.76, 231.35 HIU/25 µL respectively. The difference among them was not statistically significant at p > 0.05. In conclusion, these dogs were positive for both human and equine influenza viruses. The present study suggests the first evidence that influenza viruses circulate among dogs in Brazil.

A transmissão interespecífica do vírus influenza é relatada em estudo sobre influenza com animais domésticos desde 1970. Pássaros e mamíferos, incluindo o homem, são seus hospedeiros naturais, porém outros animais podem participar da sua epidemiologia. Foi investigada a incidência do vírus influenza em cães adultos criados em zonas rural (9, 19,56%) e urbana (37, 80,43%), do Estado de São Paulo. Os soros dos cães foram examinados pelo teste de inibição da hemaglutinação (IH), usando antígeno dos vírus influenza circulantes no Brasil. Nos cães rurais foram detectados títulos médios de 94,37, 227,88, 168,14 e 189,62 UIH/25 mL (unidades inibidoras de hemaglutinação/25 mL) para os subtipos H1N1, H3N2, H7N7, H3N8 de vírus influenza A, respectivamente, com diferenças estatisticamente significativas (p<0,05) entre as médias de títulos de anticorpos contra H1N1 e H3N2. Cerca de 84% e 92% dos cães urbanos responderam aos vírus influenza A humano H1N1 e H3N2, respectivamente e destes 92% e 100% foram positivos para os vírus eqüinos H7N7 e H3N8, respectivamente. Para esses cães as médias de títulos de anticorpos para os vírus influenza A H1N1, H3N2, H7N7 e H3N8 foram 213,96, 179,42, 231,76 e 231,35UIH/25 mL, respectivamente. As diferenças entre as médias não foram estatisticamente significativas (p>0,05). Conclui-se que os cães apresentaram positividade para ambos vírus influenza humano e equino. O presente estudo sugere, pela primeira vez, evidências de que há circulação do vírus influenza em cães, no Brasil.

Influenza viruses in adult dogs raised in rural and urban areas in the state of São Paulo, Brazil.

In 1970, searching for the interspecies transmission of influenza viruses led to the first study on influenza viruses in domestic animals. Birds and mammals, including human beings, are their natural hosts; however, other animals may also play a role in the virus epidemiology. The objective was to investigate the incidence of influenza viruses in adult dogs raised in rural (9, 19.56%) and urban (37, 80.43%) areas in the state of São Paulo, Brazil. Dog serum samples were examined for antibodies to influenza viruses by the hemagglutination inhibition (HI) test using the corresponding antigens from the circulating viruses in Brazil. Dogs from rural areas presented antibodies to influenza A H3N2, and influenza A H7N7 and H3N8. In rural areas, dog sera displayed mean titers as 94.37, 227.88, 168.14, 189.62 HIU/25 µL for subtypes H1N1, H3N2, H7N7, H3N8, respectively. About 84% and 92% of dogs from urban areas exhibited antibodies to human influenza A H1N1 and H3N2, respectively, with statistical difference at p < 0.05 between the mean titers of antibodies to H1N1 and H3N2. About 92% and 100% were positive for H7N7 and H3N8, respectively. In dogs from urban areas, the mean titers of antibodies against influenza A H1N1, H3N2, H7N7 and H3N8, were 213.96, 179.42, 231.76, 231.35 HIU/25 µL respectively. The difference among them was not statistically significant at p > 0.05. In conclusion, these dogs were positive for both human and equine influenza viruses. The present study suggests the first evidence that influenza viruses circulate among dogs in Brazil.

Antiviral effect of the egg wax of Amblyomma cajennense (Acari: Ixodidae).

The control of viral infections, especially those caused by influenza viruses, is of great interest in Public Health. Bio prospection has shown the presence of active principles in the hemolymph of arthropods, and in the salivary gland of ticks, and some of these are of interest for the development of new pharmacological drugs. Ticks lay their eggs in the environment, and to protect them from desiccation and microbial attack they involve the eggs in a waxy layer produced by an organ known as Gené's Organ. In this study, the eggs wax from tick Amblyomma cajennense (Fabricius) was extracted using ice cold phosphate buffer. The antiviral activity was evaluated with picornavirus and influenza virus. In both cases egg wax was able to inhibit virus replication. For influenza virus, an amount as small as 12 µg/mL of crude egg wax suspension neutralized 128 UHA (hemaglutinant unit) of H(1)N(1) influenza virus. With picornavirus, egg wax led to a 256-fold reduction in virus production by L929 cells. Egg wax was not cytotoxic to VERO, MDCK and L929 cell, being observed that the cell morphology was preserved with concentration as high as 2 mg/mL. In addition no genotoxic effect was observed for Vero cells, suggesting a very interesting potential antiviral activity.

VaxcineTM: an oil-based adjuvant for influenza vaccines

Vaccination is the method of choice for the prevention of influenza infection. However, the quantity of the antigen available, especially in the case of pandemics, often fails to meet the global demand. However, improved adjuvants can overcome this problem. Preliminary results obtained in this study revealed that one year after a single subcutaneous immunisation with influenza A H3N2 virus in an oil-based carrier, VaxcineTM, outbreed mice produced a high immunoglobulin G response that lasted for up to one year and exhibited less variation in titre compared with the response of the control group treated with alum. The haemagglutination-inhibition titres induced by VaxcineTM were also higher than those generated by alum. These data indicate that VaxcineTM is a good adjuvant candidate for seasonal influenza vaccines.

[Relapse into pregnancy in adolescents from Teresina, PI, Brazil]. / Reincidência da gravidez em adolescentes de Teresina, PI, Brasil.

Pregnancy in adolescence is a public health problem, and relapse rates make it even more serious. Out of 1,1 million parturient adolescents, 25% have a child already. We aimed to analyze the social economic cultural obstetric factors of relapse into pregnancy in adolescence in Teresina, PI, Brazil through interelational retrospective study. The sample contains 464 young who finished a gestation during the first four months in 2006. Out of those, 47.6% were in consensual union; 69.6% out of school; 86.9% with inappropriate schooling for the age; 72.2% with unpaid job. This profile evidences the lack of public policies that consider the adolescent. The elaboration and development of those policies should include family, school and the community in order to decrease pregnancy and sexually-transmited diseases rates.

Reincidência da gravidez em adolescentes de Teresina, PI, Brasil / Relapse into preganancy in adolescents from Teresina, PI, Brazil / Reincidencia del embarazo en adolescentes de Teresina, PI, Brasil

A gravidez na adolescência é um problema de saúde pública, tornando-se mais grave em virtude da reincidência. De 1,1 milhões de parturientes adolescentes, 25 por cento tem um filho. Objetivou-se analisar os fatores sócio-econômico-cultural e obstétrico da reincidência de gravidez na adolescência em Teresina, PI, Brasil por meio de um estudo interrelacional retrospectivo. A amostra incluiu 464 jovens que finalizaram uma gestação no primeiro quadrimestre de 2006. Destas, 47,6 por cento encontrava-se em união consensual; 69,6 por cento fora da escola; 86,9 por cento com escolaridade inadequada para a idade; 72,2 por cento sem trabalho remunerado. Este perfil evidencia a escassez de políticas públicas que contemplem o adolescente. A elaboração e efetivação dessas políticas deveriam incluir família, escola e comunidade para redução dos indicadores de gravidez e doenças sexualmente transmissíveis.

Pregnancy in adolescence is a public health problem, and relapse rates make it even more serious. Out of 1,1 million parturient adolescents, 25 percent have a child already. We aimed to analyze the social economic cultural obstetric factors of relapse into pregnancy in adolescence in Teresina, PI, Brazil through interelational retrospective study. The sample contains 464 young who finished a gestation during the first four months in 2006. Out of those, 47.6 percent were in consensual union; 69.6 percent out of school; 86.9 percent with inappropriate schooling for the age; 72.2 percent with unpaid job. This profile evidences the lack of public policies that consider the adolescent. The elaboration and development of those policies should include family, school and the community in order to decrease pregnancy and sexually-transmited diseases rates.

El embarazo en la adolescencia es un problema de salud pública, tornándose más grave en virtud de la reincidencia. De 1,1 millones de parturientes adolescentes, 25 por ciento ya tienen un hijo. Objetivamos analizar los factores socios económicos culturales y obstétricos de la reincidencia del embarazo en la adolescencia en Teresina, PI, Brazil por medio de un estudio interrelacional retrospectivo. La muestra incluye 464 jóvenes que finalizaron una gestación en el primer cuadrimestre de 2006. De estas, 47,6 por ciento estaban en unión consensual; 69.6 por ciento fuera de la escuela; 86,9 por ciento con escolaridad inadecuada para la edad; 72,2 por ciento sin trabajo remunerado. Este perfil evidencia la escasez de políticas públicas que contemplen el adolescente. La elaboración y efectuación de esas políticas deberían incluir la familia, la escuela y la comunidad para reducción de los indicadores de embarazo y enfermedades sexualmente transmisibles.

Vaxcine(TM): an oil-based adjuvant for influenza vaccines.

Vaccination is the method of choice for the prevention of influenza infection. However, the quantity of the antigen available, especially in the case of pandemics, often fails to meet the global demand. However, improved adjuvants can overcome this problem. Preliminary results obtained in this study revealed that one year after a single subcutaneous immunisation with influenza A H3N2 virus in an oil-based carrier, Vaxcine(TM), outbreed mice produced a high immunoglobulin G response that lasted for up to one year and exhibited less variation in titre compared with the response of the control group treated with alum. The haemagglutination-inhibition titres induced by Vaxcine(TM) were also higher than those generated by alum. These data indicate that Vaxcine(TM) is a good adjuvant candidate for seasonal influenza vaccines.

Análise da produção científica sobre o uso dos métodos contraceptivos pelos adolescentes / Analysis of scientific production on the usage of contraceptive methods by adolescents / Análisis de la producción cientifica sobre el uso de los métodos contraceptivos por los adolescentes

Este estudo objetivou analisar a produção científica nacional relacionada aos contraceptivos na adolescência. Realizou-se uma pesquisa quantitativa, descritiva, por meio da revisão sistemática da literatura, no Banco de dados da CAPES de 1997 a 2007. Dos 43 resumos, 35 são dissertações (81,4 por cento) e oito (18,4 por cento) teses. Entre 1997 a 2002 verificou-se um maior número de publicações. A área da saúde destacou-se com 58,1 por cento e predominaram os estudos qualitativos (48,8 por cento). Quanto à ênfase, evidenciou-se o conhecimento e uso de contraceptivos e as opiniões e/ou percepções sobre a saúde sexual e reprodutiva dos adolescentes. Os profissionais de diversas áreas, principalmente os da saúde, mostraram-se preocupados com a temática, sugerindo estratégias de vida sexual e reprodutiva de forma saudável.

The purpose fo the study was to analyze national scientific production on the contraceptives in adolescence. A quantitative descriptive research through systematic revision was performed in CAPES' database from 1997 to 2007. Out of the 43 ABSTRACTs, 35 were dissertations (81.4 percent) and eigth (18.4 percent) were doctoral theses. From 1997 to 2002 there was a wide number of publications. The health area presented 58.1 percent and the qualitative studies were predominant (48.8 percent). When it comes to the focus, the knowledge and usage of contraceptives and the opinions and/or perceptions on sexual and reproductive health of adolescents were highlighted. The professionals from several fields are concerned about discussing the theme, suggesting strategies of how adolescents should live their sexual and reproductive lives in a healthy way.

Este estudio objetivó analisar la producción cientifica nacional relacionada a los contraceptivos en la adolescencia. Una pesquisa cualitativa y descriptiva a través de revisión sistemática en el Banco de datos de CAPES de 1997 a 2007 fue realizada. De los 43 resúmenes, 35 son disertaciones (81,4 por ciento) y 98 (18,4 por ciento) son tesis doctorales. Entre 1997 a 2002 fue verificado un número mayor de publicaciones. La área de la salud destacóse con 58,1 por ciento y predominaron los estudios cualitativos (48,8 por ciento). Cuanto al foco, el conocimiento y uso de contraceptivos y las opiniones y/o percepciones sobre la salud sexual y reproductiva de los adolescentes fueron evidenciados. Los profesionales de diversas áreas están preocupados en discutir el tema, sugerindo estrategias de como los adolescentes puedan vivir su vida sexual y reproductiva de una forma saludable.

[Analysis of scientific production on the usage of contraceptive methods by adolescents]. / Análise da produção científica sobre o uso dos métodos contraceptivos pelos adolescentes.

The purpose fo the study was to analyze national scientific production on the contraceptives in adolescence. A quantitative descriptive research through systematic revision was performed in CAPES' database from 1997 to 2007. Out of the 43 ABSTRACTs, 35 were dissertations (81.4%) and eighth (18.4%) were doctoral theses. From 1997 to 2002 there was a wide number of publications. The health area presented 58.1% and the qualitative studies were predominant (48.8%). When it comes to the focus, the knowledge and usage of contraceptives and the opinions and/or perceptions on sexual and reproductive health of adolescents were highlighted. The professionals from several fields are concerned about discussing the theme, suggesting strategies of how adolescents should live their sexual and reproductive lives in a healthy way.