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Aliphatics prevail in asteroids, comets, meteorites and other bodies in our solar system. They are also found in the interstellar and circumstellar media both in gas-phase and in dust grains. Among aliphatics, linear alkanes (n-CnH2n+2) are known to survive in carbonaceous chondrites in hundreds to thousands of parts per billion, encompassing sequences from CH4 to n-C31H64. Despite being systematically detected, the mechanism responsible for their formation in meteorites has yet to be identified. Based on advanced laboratory astrochemistry simulations, we propose a gas-phase synthesis mechanism for n-alkanes starting from carbon and hydrogen under conditions of temperature and pressure that mimic those found in carbon-rich circumstellar envelopes. We characterize the analogs generated in a customized sputter gas aggregation source using a combination of atomically precise scanning tunneling microscopy, non-contact atomic force microscopy and ex-situ gas chromatography-mass spectrometry. Within the formed carbon nanostructures, we identify the presence of n-alkanes with sizes ranging from n-C8H18 to n-C32H66. Ab-initio calculations of formation free energies, kinetic barriers, and kinetic chemical network modelling lead us to propose a gas-phase growth mechanism for the formation of large n-alkanes based on methyl-methylene addition (MMA). In this process, methylene serves as both a reagent and a catalyst for carbon chain growth. Our study provides evidence of an aliphatic gas-phase synthesis mechanism around evolved stars and provides a potential explanation for its presence in interstellar dust and meteorites.
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OBJECTIVE: To study the clinical characteristics of macular diplopia, treatment, and outcome. METHODS: Retrospective descriptive study of cases referred to the ocular motility section of a tertiary hospital with diplopia, diagnosed with macular diplopia between 2022-23. The etiology of the macular pathology and the type of associated strabismus were recorded. The result was considered good if the diplopia improved or was eliminated with the medical or surgical treatment. Follow-up time from the onset of diplopia until data collection was recorded. RESULTS: a total of 19 cases comprised the sample (63.2% women), mean age: 67.16 years. Amblyopia (21.1%), high myopia (47.4%), epirretinal membrane (ERM) (36.8%), neovascular membrane (26.3%), macular hole (10.5%), and lamellar (15.8%), and age macular degeneration (5.3%) were registered. The 47.4% had vertical diplopia, horizontal: 5.3 and 47.4% mixed. The mean horizontal deviation was: 7.3 PD (prism diopters) and vertical: 6.22 PD. Ocular extorsion was observed in 26.3%, and intorsion: 5.3%. Torticollis was present in 15.8%. The treatment consisted of strabismus surgery + Botox (15.8%), strabismus surgery (47.4%), medical treatment with Fresnel prims or Scotch cellophane (36.8%). A 68.4% presented a good result at the end of the study. The mean follow-up was 55.58 months. CONCLUSIONS: Misregistration of macular photoreceptors is the most common cause of binocular diplopia in patients with ERM or other macular pathologies. Most complains of vertical or mixed diplopia. Sensorimotor evaluation of these patients should be thorough. Early diagnosis prevents unnecessary prescription of prism glasses. Surgical and/or medical treatment achieves good results in most cases.
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Diplopía , Estrabismo , Humanos , Femenino , Masculino , Estudios Retrospectivos , Estrabismo/etiología , Anciano , Diplopía/etiología , Persona de Mediana Edad , Resultado del Tratamiento , Anciano de 80 o más Años , Adulto , Mácula Lútea , Enfermedades de la Retina/complicaciones , Ambliopía/etiología , Ambliopía/terapiaRESUMEN
PURPOSE: To describe the outcome of the patients diagnosed of sagging/heavy eye associated to myopia, that were operated on with the supra-equatorial displacement with LR myopexy. METHODS: A retrospective study of 9 cases between 2017-2023. The following data were analyzed: horizontal and vertical deviation, diplopia, amblyopia, ductions, ocular torsion, sensorial test, macular pathology, and the orbital magnetic resonance. Treatment was considered Successful if the diplopia was improved or eliminated and a final vertical deviation (VD) ≤5 prism diopters (PD). RESULTS: The mean age (SD) was: 62.11 (4.6) years (100% women). A total of 88.88% presented diplopia. The mean preoperative hypotropia was: 11.33 PD (SD 3.16), and the mean final VD 3.44 PD (SD 3.05). After surgery, the hypotropia was overcorrected in one case, under corrected in 5, and orthophoria was achieved in another three. The technique was associated with surgery of another rectus muscle in 4 subjects. The mean follow-up time after surgery was 34 months (SD 34.62). Six of the 9 patients improved with a vertical deviation ≤5 PD. In 3 patients, the diplopia was eliminated, while in 5 it remained intermittent (three with macular pathology). CONCLUSION: Supra-equatorial displacement with LR myopexy for treatment of myopic sagging/heavy eye, is a therapeutic option if hypotropia is less than 12 PD or the Yokoyama technique is not indicated. A good result was obtained in most cases, although diplopia could only be totally suppressed in three, and another five remained intermittent.
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Miopía , Estrabismo , Humanos , Femenino , Persona de Mediana Edad , Masculino , Diplopía/etiología , Diplopía/cirugía , Estudios Retrospectivos , Procedimientos Quirúrgicos Oftalmológicos/métodos , Miopía/complicaciones , Miopía/cirugía , Músculos Oculomotores/cirugía , Músculos Oculomotores/patología , Estrabismo/etiología , Estrabismo/cirugíaRESUMEN
OBJECTIVE: To study the outcomes of treatment with central mini-plication of the medial rectus (MR) muscles in adult convergence insufficiency with diplopia. METHODS: The study sample comprised 9 cases (7 women). The length of MR plication was collected. Other variables reported were postsurgical deviation, overcorrections in the early postoperative period and at the end of follow-up, final horizontal deviation at near and at distance vision, diplopia, and mean follow-up from surgery. Surgical outcome was considered to be favorable when diplopia and symptoms were resolved and final exotropia at near was ≤8â¯pd at the end of follow-up. RESULTS: A central mini-plication of the MR was performed in 9 patients (8 unilateral). Mean (±SD) age was 58.66 (21.39) years. Mean near preoperative deviation: 16.22 (±2.9) pd and distance preoperative deviation: 6.88 (±4) pd Overcorrection at distance vision was recorded in 5 cases; this resolved by 3 months postoperatively. There was not overcorrection at near vision except one case. None of the cases operated on had overcorrection at the end of follow-up. The final horizontal deviation was ≤8â¯pd at near vision, except for 3 cases (mean: 6.22). Symptoms and diplopia resolved in 8 cases. The mean follow-up was 10.33 months. CONCLUSION: Central mini-plication of 1 or 2 medial rectus muscles can improve the symptoms and signs of exotropia associated with convergence insufficiency when exercises and the prisms are rejected by the patients and when these approaches have not solved the problem.
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Exotropía , Miopía , Trastornos de la Motilidad Ocular , Humanos , Adulto , Femenino , Persona de Mediana Edad , Exotropía/cirugía , Resultado del Tratamiento , Diplopía/etiología , Diplopía/cirugía , Procedimientos Quirúrgicos Oftalmológicos , Estudios Retrospectivos , Trastornos de la Motilidad Ocular/cirugía , Miopía/cirugíaRESUMEN
PURPOSE: To evaluate the efficacy and safety of inferior oblique muscle transposition and myopexy in patients with mild/moderate inferior oblique muscle overaction, with or without diplopia. METHOD: We retrospectively analysed data for the 12 patients who underwent the technique. Data were collected from October 2018 to September 2021. Surgery was performed by suturing the inferior oblique belly to the sclera at 5â¯mm posterior to the temporal end of the inferior rectus. All 12 patients had mild hypertropia (≤6 prism diopters [pd]) in primary position and mild/moderate inferior oblique overaction. Mean preoperative hypertropia was 4.42 pd⯱â¯1.62. Diplopia was recorded in 10 cases. The diagnoses were fourth nerve paresis (9), unilateral primary inferior oblique overaction (2) and dissociated vertical deviation (1). Torticollis was observed in 7 cases, 2 had subjective torsion and 2 objective torsion. RESULTS: Mean age was 46.86⯱â¯25.1 years (50%: men). Diplopia resolved in 9 of the 10 cases. The mean final vertical deviation was 1.5⯱â¯2.93 (pâ¯=â¯0.001) pd in straight gaze. Of 7 mild overshoot in adduction, it disappeared in 3 and 4 remained the same. Of 5 moderate overshoot in adduction, 2 improved to mild and 3 disappeared. Torticollis was eliminated in 5 patients and improved in another 2. Mean time from surgery was 14.08⯱â¯8.05 months. There were no overcorrections. CONCLUSIONS: Inferior oblique muscle transposition with myopexy is a safe and effective procedure in patients with mild-to-moderate inferior oblique muscle overaction and small-angle hypertropia, with or without diplopia.
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Trastornos de la Motilidad Ocular , Enfermedades Orbitales , Estrabismo , Tortícolis , Adulto , Anciano , Diplopía/etiología , Diplopía/cirugía , Humanos , Masculino , Persona de Mediana Edad , Músculos Oculomotores/cirugía , Estudios Retrospectivos , Estrabismo/cirugía , Resultado del Tratamiento , Visión Binocular/fisiología , Adulto JovenRESUMEN
PURPOSE: To study the results of surgical treatment of acquired concomitant esotropia and its posible causes. METHODS: Retrospective study of the patients with acute acquired esotropia that were operated on since 2017. Distance and near horizontal deviation, spherical equivalent and the excessive use of the tablets and smartphones were analyzed. A good sensorial outcome was considered when stereopsis was recovered and good motor outcome if horizontal deviation was ≤10 prism diopters at the end of the follow-up. RESULTS: A total of 15 cases were included (60% women), mean age: 29.07 years (86.66% ≥16 years). Mean time since the beginning of the symptoms to the surgery was 23.3 months. Mean horizontal preoperative deviation was 19.73 ± 7.2 pd at near and 22.93 ± 7.3 dp at distance decreasing to 3.33 ± 3.8 pd at near and 3.86 ± 3.9 pd at distance (P < .001). The 66.6% had diplopía. The 46.66% had decompensated esophoria or microesotropia, 86,66 % myopia and the 33.33% referred excesive use of the tablets and smartphones. The medial rectus recession were done in 10 cases. A good motor outcome was obtained in the 93.33% of the cases and sensorial outcome in the 53.3% without diplopía. CONCLUSION: Surgery was an excellent treatment to resolve the deviation and diplopia. The esophorias and microesotropias represented near the half of the cases and the excesive use of the tablets and smartphones were the third part of the sample. Most of the patients had myopia.
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Esotropía , Miopía , Enfermedad Aguda , Adulto , Diplopía/cirugía , Esotropía/diagnóstico , Esotropía/etiología , Esotropía/cirugía , Femenino , Humanos , Masculino , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
[This corrects the article DOI: 10.1093/ofid/ofab250.].
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Circular economy principle aims to achieve sustainable production systems, focusing on the waste valorisation and the reduction of gaseous losses to the atmosphere. Nitrogen (N) compounds in terms of ammonia (NH3) and nitrous oxide (N2O) represent the major losses to the atmosphere of laying hen manure management chain. We present a study aimed to evaluate NH3 and N2O emission and mitigation strategies at housing, storage and land spreading stages. The whole manure management chain was evaluated under different scenarios which combined mitigation strategies of each stage. Two intensive laying hen facilities were involved in the study. Evaluated mitigation strategies were: (i) frequency of manure removal from housing facility, (ii) dried manure storage after passing throughout a manure drying tunnel (MDT) compared to fresh manure storage and (iii) fresh or dried manure incorporation versus surface land application. Increasing the frequency of manure removal from 4 days to 1/3 daily, reduced N losses around 68%. Dried manure storage achieved around 75% reduction in N losses compared to fresh manure storage. Spreading dried manure on grassland surface reduced ≈77% NH3 losses in relation to the emission level reached by fresh manure. The reduction was similar when dried manure was incorporated compared to surface application of fresh manure (≈79%). A 40% reduction in N losses was achieved using the MDT compared to no drying strategy. In the whole manure management chain, the combination of strategies that most reduced N losses was: removal frequency of 1/3 daily, dry storage after passing through the MDT and incorporated land application. These strategies reduced N losses between 40 and 60% compared to the 4 days of removal frequency, fresh storage and surface application of fresh manure.
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Estiércol , Óxido Nitroso , Amoníaco/análisis , Animales , Pollos , Granjas , Femenino , Óxido Nitroso/análisisRESUMEN
OBJECTIVE: The perform pre-clinical testing using optical design tools to simulate the optical quality of a smart artificial iris platform encapsulated in a scleral contact lens. These tools allow us to generate aniridia eye models and evaluate different metrics of visual quality and retinal illumination based on the aperture of the artificial iris based on liquid crystals. METHOD: The OCT imaging technique was used to measure the geometry of the anterior segment in a patient with aniridia and, from these data, the eye model was generated with the Zemax optical design program and specific programs developed in Matlab. Ocular aberrations were calculated and the visual function of the anirida eye model was evaluated in three scenarios: (i) without optical correction, (ii) with correction with a commercial scleral contact lens, and (iii) with correction with an optical lens. Intelligent contact based on artificial iris. RESULTS: Optical quality in patients with aniridia is limited by the magnitude of high-order aberrations. Conventional scleral contact lens design accurately corrects for blur but is unable to compensate for high-order ocular aberrations, especially spherical aberrations. The artificial iris-based smart contact lens design enables virtually all high-order aberrations to be compensated with active control of the pupillary diameter (activation of liquid crystal cells based on ambient lighting). In addition to minimizing high-order aberrations, reducing the pupil size would increase the depth of focus. CONCLUSIONS: This article demonstrates by means of optical simulations the concept of an intelligent artificial iris platform encapsulated in a scleral contact lens and its possible application in patients with aniridia. Furthermore, it allows us to anticipate possible visual results in clinical trials with healthy patients (after application of mydriatic agents) and in patients with aniridia. The results demonstrate a better visual quality and a decrease in retinal illumination.
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Aniridia , Lentes de Contacto , Lentes Intraoculares , Aniridia/terapia , Humanos , Iris , Agudeza VisualRESUMEN
BACKGROUND: There are no clear criteria for antifungal de-escalation after initial empirical treatments. We hypothesized that early de-escalation (ED) (within 5 days) to fluconazole is safe in fluconazole-susceptible candidemia with controlled source of infection. METHODS: This is a multicenter post hoc study that included consecutive patients from 3 prospective candidemia cohorts (2007-2016). The impact of ED and factors associated with mortality were assessed. RESULTS: Of 1023 candidemia episodes, 235 met inclusion criteria. Of these, 54 (23%) were classified as the ED group and 181 (77%) were classified as the non-ED group. ED was more common in catheter-related candidemia (51.9% vs 31.5%; Pâ =â .006) and episodes caused by Candida parapsilosis, yet it was less frequent in patients in the intensive care unit (24.1% vs 39.2%; Pâ =â .043), infections caused by Nakaseomyces glabrata (0% vs 9.9%; Pâ =â .016), and candidemia from an unknown source (24.1% vs 47%; Pâ =â .003). In the ED and non-ED groups, 30-day mortality was 11.1% and 29.8% (Pâ =â .006), respectively. Chronic obstructive pulmonary disease (odds ratio [OR], 3.97; 95% confidence interval [CI], 1.48-10.61), Pitt scoreâ >â 2 (OR, 4.39; 95% CI, 1.94-9.20), unknown source of candidemia (OR, 2.59; 95% CI, 1.14-5.86), candidemia caused by Candida albicans (OR, 3.92; 95% CI, 1.48-10.61), and prior surgery (OR, 0.29; 95% CI, 0.08-0.97) were independent predictors of mortality. Similar results were found when a propensity score for receiving ED was incorporated into the model. ED had no significant impact on mortality (OR, 0.50; 95% CI, 0.16-1.53). CONCLUSIONS: Early de-escalation is a safe strategy in patients with candidemia caused by fluconazole-susceptible strains with controlled source of bloodstream infection and hemodynamic stability. These results are important to apply antifungal stewardship strategies.
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OBJECTIVE: To perform pre-clinical testing using optical design tools to simulate the optical quality of a smart artificial iris platform encapsulated in a scleral contact lens. These tools allow us to generate aniridia eye models and evaluate different metrics of visual quality and retinal illumination based on the aperture of the artificial iris based on liquid crystals. METHOD: The OCT imaging technique was used to measure the geometry of the anterior segment in a patient with aniridia and, from these data, the eye model was generated with the Zemax optical design program and specific programs developed in Matlab. Ocular aberrations were calculated and the visual function of the anirida eye model was evaluated in three scenarios: (i) without optical correction, (ii) with correction with a commercial scleral contact lens, and (iii) with correction with an optical lens. intelligent contact based on artificial iris. RESULTS: Optical quality in patients with aniridia is limited by the magnitude of high-order aberrations. Conventional scleral contact lens design accurately corrects for blur but is unable to compensate for high-order ocular aberrations, especially spherical aberrations. The artificial iris-based smart contact lens design enables virtually all high-order aberrations to be compensated with active control of the pupillary diameter (activation of liquid crystal cells based on ambient lighting). In addition to minimizing high-order aberrations, reducing the pupil size would increase the depth of focus. CONCLUSIONS: This article demonstrates by means of optical simulations the concept of an intelligent artificial iris platform encapsulated in a scleral contact lens and its possible application in patients with aniridia. Furthermore, it allows us to anticipate possible visual results in clinical trials with healthy patients (after application of mydriatic agents) and in patients with aniridia. The results demonstrate a better visual quality and a decrease in retinal illumination.
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The hexagonal close packed surface of gold shows a 22 × 3 "herringbone" surface reconstruction which makes it unique among the (111) surfaces of all metals. This long-range energetically favored dislocation pattern appears in response to the strong tensile stress that would be present on the unreconstructed surface. Adsorption of molecular and atomic species can be used to tune this surface stress and lift the herringbone reconstruction. Here we show that herringbone reconstruction can be controllably lifted in ultrahigh vacuum at cryogenic temperatures by precise hot electron injection in the presence of hydrogen molecules. We use the sharp tip of a scanning tunneling microscope (STM) for charge carrier injection and characterization of the resulting chain nanostructures. By comparing STM images, rotational spectromicroscopy and ab initio calculations, we show that formation of gold atomic chains is associated with release of gold atoms from the surface, lifting of the reconstruction, dissociation of H2 molecules, and formation of surface hydrides. Gold hydrides grow in a zipper-like mechanism forming chains along the [11Ì 0] directions of the Au(111) surface and can be manipulated by further electron injection. Finally, we demonstrate that Au(111) terraces can be transformed with nearly perfect terrace selectivity over distances of hundreds of nanometers.
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Kabuki syndrome is a rare genetic disorder, caused by mutation in the KMT2D or KDM6A genes, which affects several organs in the majority of patients, among which are the eyes. The most typical clinical characteristics are mental retardation, postnatal growth retardation, skeletal anomalies, and characteristic facial features. As the eyes are affected in most of the cases, ophthalmological examination is recommended for the early detection of ocular anomalies, in order to prevent visual impairment. The most frequent ocular signs are strabismus, ptosis, and refractive anomalies. A series of cases of Kabuki syndrome is described in five children, four of whom exhibited strabismus with esotropia, over action of inferior oblique muscles, and under action of superior oblique muscles associated with a V pattern. Most published papers do not report or might underestimate the ocular problems. It may be appropriate to perform orbital magnetic resonances in order to detect changes in the muscle paths that are related to the pathology of the eye movements found.
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Anomalías Múltiples/fisiopatología , Cara/anomalías , Enfermedades Hematológicas/fisiopatología , Trastornos de la Motilidad Ocular/genética , Enfermedades Vestibulares/fisiopatología , Anomalías Múltiples/genética , Astigmatismo/genética , Blefaroptosis/genética , Niño , Preescolar , Proteínas de Unión al ADN/genética , Cara/fisiopatología , Femenino , Mutación del Sistema de Lectura , Enfermedades Hematológicas/genética , Humanos , Hiperopía/genética , Masculino , Proteínas de Neoplasias/genética , Estrabismo/genética , Estrabismo/cirugía , Enfermedades Vestibulares/genéticaRESUMEN
The original Rivermead Behavioural Memory Test (RBMT) suggests a cutoff global score of 10 points. However, this limit may be too stringent for older adults attending memory training programs, particularly for those with low education levels. This study aims to provide appropriately adjusted age and education norms for the RBMT. Data from 711 subjects were grouped based on age (65-67, 68-71, 72-75 and 76-83) and education level (primary school, high school and university studies). The data exhibit a clear trend of scores decreasing with age. The diminution in scores does not reach trend levels of significance between neighboring (< 5 years) age intervals, but it is statistically significant at the designated alpha level (p = .05) when younger and older adults are compared over a range of 5 years of difference. 81.86% of our sample has global scores below the suggested cutoff of 10 points. The present study provides a more accurate representation of RBMT global score performance in older adults for specific age and education stratifications.
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INTRODUCTION: Semantic content processing is associated with the potential N400, and the P600 is linked with the processing of syntactic and grammatical rules. AIM: To verify whether the semantic processing of complex visual stimuli such as repetition, identity, order and double incongruence is recursive or computable. SUBJECTS AND METHODS: 27 university students responded to an adapted N400 paradigm with five conditions, each with 80 tasks, while recording their brain activity with a 64-channel cap. RESULTS: Two temporal windows of 400 to 550 ms and 550 to 800 ms were analyzed using an ANOVA contrast of the condition factor by regions of interest. In addition, the inverse solution of the windows was calculated by low resolution electromagnetic tomography (LORETA) to identify the main sources related to the electrical power. The significant differences (p <0.05) in the results for the N400 and P600 in frontal and centroparietal areas in the logical operators studied are corroborated. CONCLUSION: It is confirmed that the brain processing of complex images is modulated by repetition, identity and order, but not by negation. Therefore, it can be said that the semantic processing of complex images is semi-computable.
TITLE: Localizacion cerebral del procesamiento semantico.Introduccion. El procesamiento del contenido semantico se asocia al potencial N400, y el P600 se vincula con el procesamiento de reglas sintacticas y gramaticales. Objetivo. Verificar si el procesamiento semantico de estimulos visuales complejos, como la repeticion, la identidad, el orden y la doble incongruencia, es recursivo o computable. Sujetos y metodos. Veintisiete universitarios respondieron a un paradigma adaptado N400 con cinco condiciones, cada una con 80 tareas, mientras se registraba su actividad cerebral con un gorro de 64 electrodos. Resultados. Dos ventanas temporales de 400 a 550 ms y de 550 a 800 ms se analizaron mediante un contraste ANOVA del factor condicion por regiones de interes. Ademas, se calculo la solucion inversa de las ventanas mediante tomografia electromagnetica de baja resolucion para identificar las fuentes corticales subyacentes a los potenciales electricos. Se corroboran diferencias significativas (p < 0,05) en los potenciales N400 y P600 en areas frontales y centroparietales asociadas a los operadores logicos estudiados. Conclusion. Se confirma que el procesamiento cerebral de imagenes complejas (congruentes/incongruentes) es modulado por la repeticion, la identidad y el orden, pero no por la negacion. Por consiguiente, se puede decir que el procesamiento semantico de imagenes complejas es semicomputable.
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Mapeo Encefálico/métodos , Encéfalo/fisiología , Potenciales Evocados , Semántica , Adulto , Femenino , Humanos , Masculino , Adulto JovenRESUMEN
To determine Toll-like receptors (TLR)2 and TLR4 expression levels and associate them with matrix metalloproteinases (MMPs) in asymptomatic apical periodontitis (AAP), symptomatic apical periodontitis (SAP), and healthy controls. Apical tissue/lesion samples were obtained from chronic AAP (n = 35) and SAP (n = 29), and healthy periodontal ligament (HPL, n = 10) with indication of tooth extraction, respectively. mRNA expression levels of TLR2, TLR4, MMP-1, MMP-2, MMP-8, and MMP-13 were determined by real-time reverse-transcription polymerase chain reaction. The data were analyzed with Kruskal-Wallis and Dunn's pot hoc test (p < 0.05). The correlation coefficient was obtained using the Spearman correlation (p < 0.05). TLR2, MMP-1, MMP-2, and MMP-13 mRNA levels were the highest in SAP followed by AAP and controls (p < 0.05). TLR4 and MMP-8 were over expressed in AAP and SAP compared to HPL (p < 0.05). TLR2 positively correlated with TLR4, MMP-1, MMP-8, and MMP-13 in SAP (p < 0.05). TLR2 and TLR4 are overexpressed in apical lesions versus healthy periodontal ligament and correlate with collagenolytic MMPs. Particularly, TLR2 is overexpressed in SAP in association with MMP-1, MMP-8, and MMP-13. Our results suggest that the activation of TLR2 along with MMP overexpression might contribute to SAP clinical presentation and progression. TLRs, MMPs, and their interaction can explain the clinical presentations and evolution of apical periodontitis and might represent key targets for new diagnostic and treatment approaches.
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Metaloproteinasas de la Matriz/metabolismo , Periodontitis Periapical/metabolismo , Receptor Toll-Like 2/metabolismo , Receptor Toll-Like 4/metabolismo , Estudios Transversales , Humanos , Periodontitis Periapical/patología , Ligamento Periodontal/efectos de los fármacos , Ligamento Periodontal/metabolismo , Receptor Toll-Like 2/genética , Receptor Toll-Like 4/genética , Ápice del Diente/metabolismoRESUMEN
Gaseous emission in laying hen facilities affects animal production performance and the environment. Emission is ruled by gas concentration and ventilation rate (VR), which are the key parameters to estimate precise emission factors. In this work, VR were assessed in a mechanical ventilated laying hen facility under Mediterranean climate conditions. The study was performed during a complete production cycle from July 2015 to October 2016. Direct hot wire anemometer (HWA) and fan rotational speed methods, and indirect CO2 mass balance method were used to assess the VR. Mean VR was 5.3 ± 2.9 m3 h-1 hen-1 for the HWA method. The mean uncertainty of VR based on the HWA method was 8.5%, and it varied among ventilation stages from 2.3 to 12.8%. Uncertainty was higher in warm season (9.6%) than in cold season (5.4%). In relation to fan rotational speed method, mean VR was 5.9 ± 3.3 m3 h-1 hen-1, which accounts for 8.3% more than the HWA method. According to the results, the fan rotational speed method would be less sensitive than the HWA method for assessing VR. VR was 6.3 ± 2.1 m3 h-1 hen-1 for the CO2 balance method, which estimated lower VR values in warm season compared to the laser tachometer (LT) method (-5%) but higher values compared to the HWA method (+5%). In contrast, it estimated higher values in cold season compared to the HWA method (+23%) and LT method (+17%). Correlation analysis showed a strong correlation between the CO2 balance method and both direct methods (R = 0.85 and 0.86 for HWA and LT, respectively).
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Monitoreo del Ambiente/métodos , Vivienda para Animales , Ventilación , Animales , Pollos , Femenino , EspañaRESUMEN
Aluminum consumption has been associated with various neurodegenerative diseases. Previous studies suggest that regular beer intake reverses the pro-oxidant and inflammatory statuses induced by aluminum nitrate intoxication. This paper aims to evaluate the in vitro antioxidant capacity and acetylcholinesterase inhibitory activity of non-alcoholic beer (NABeer), silicon or hops, as well as their effect on animal behavior (e.g. curiosity, immobilization, rearing, grooming, swimming) and brain antioxidant enzyme (activity and gene expression) and anti-inflammatory status in aluminum nitrate intoxicated rats. Male Wistar rats were divided into five groups: 1) Control, 2) Aluminum nitrate (450⯵g/kg/day), 3) Aluminum nitrate plus NABeer, 4) Aluminum nitrate plus hops, and 5) Aluminum nitrate plus silicon. Hops showed the highest in vitro antioxidant capacity and silicon the highest anticholinesterase activity. In the Aluminum group the brain aluminum/silicon ratio increased with impairment of brain antioxidant and inflammatory statuses. NABeer, silicon and hops block the negative effect on the in vivo antioxidant and inflammatory statuses induced by Aluminum nitrate and improve swimming and rearing behavioral tests. The various positive results suggest that NABeer is useful as a functional multi-target drink in the prevention of some neurodegenerative events caused by aluminum intoxication. More studies are required to conclude present results.
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Compuestos de Aluminio/toxicidad , Antioxidantes/farmacología , Conducta Animal/efectos de los fármacos , Bebidas , Encéfalo/efectos de los fármacos , Inhibidores de la Colinesterasa/farmacología , Humulus , Nitratos/toxicidad , Silicio/farmacología , Acetilcolinesterasa/efectos de los fármacos , Animales , Encéfalo/patología , Butirilcolinesterasa/efectos de los fármacos , Inflamación/terapia , Masculino , Memoria/efectos de los fármacos , Actividad Motora/efectos de los fármacos , Ratas WistarRESUMEN
Enantiopure 2-thia-4-azabicyclo[3.1.0]hexanes, which can be considered constrained cysteines, have been obtained from l-cysteine by application of the "self-regeneration of chirality" concept. The key intermediates are homochiral thiazolines that can be prepared in multigram scale and react smoothly with a series of diazoalkanes providing Δ1-pyrazolines except for phenyldiazomethane that yield the isomeric Δ2-pyrazolines. Nitrogen extrusion in Δ1-pyrazolines and further reduction of the sulfinyl group yielded the target compounds in good overall yield. Computational studies of the cycloaddition reaction were used for determining the polarity of the process and explaining the observed stereoselectivity. Additional topological studies were employed for determining the influence of noncovalent interactions in the stereochemical course of the reaction, which showed to be a highly asynchronous concerted process.