RESUMEN
Disrupting inositol 1,4,5-trisphosphate (IP3) receptor (IP3R)/B-cell lymphoma 2 (Bcl-2) complexes using a cell-permeable peptide (stabilized TAT-fused IP3R-derived peptide (TAT-IDP(S))) that selectively targets the BH4 domain of Bcl-2 but not that of B-cell lymphoma 2-extra large (Bcl-Xl) potentiated pro-apoptotic Ca(2+) signaling in chronic lymphocytic leukemia cells. However, the molecular mechanisms rendering cancer cells but not normal cells particularly sensitive to disrupting IP3R/Bcl-2 complexes are poorly understood. Therefore, we studied the effect of TAT-IDP(S) in a more heterogeneous Bcl-2-dependent cancer model using a set of 'primed to death' diffuse large B-cell lymphoma (DL-BCL) cell lines containing elevated Bcl-2 levels. We discovered a large heterogeneity in the apoptotic responses of these cells to TAT-IDP(S) with SU-DHL-4 being most sensitive and OCI-LY-1 being most resistant. This sensitivity strongly correlated with the ability of TAT-IDP(S) to promote IP3R-mediated Ca(2+) release. Although total IP3R-expression levels were very similar among SU-DHL-4 and OCI-LY-1, we discovered that the IP3R2-protein level was the highest for SU-DHL-4 and the lowest for OCI-LY-1. Strikingly, TAT-IDP(S)-induced Ca(2+) rise and apoptosis in the different DL-BCL cell lines strongly correlated with their IP3R2-protein level, but not with IP3R1-, IP3R3- or total IP3R-expression levels. Inhibiting or knocking down IP3R2 activity in SU-DHL-4-reduced TAT-IDP(S)-induced apoptosis, which is compatible with its ability to dissociate Bcl-2 from IP3R2 and to promote IP3-induced pro-apoptotic Ca(2+) signaling. Thus, certain chronically activated B-cell lymphoma cells are addicted to high Bcl-2 levels for their survival not only to neutralize pro-apoptotic Bcl-2-family members but also to suppress IP3R hyperactivity. In particular, cancer cells expressing high levels of IP3R2 are addicted to IP3R/Bcl-2 complex formation and disruption of these complexes using peptide tools results in pro-apoptotic Ca(2+) signaling and cell death.
Asunto(s)
Apoptosis/efectos de los fármacos , Receptores de Inositol 1,4,5-Trifosfato/metabolismo , Péptidos/farmacología , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Calcio/metabolismo , Línea Celular Tumoral , Humanos , Receptores de Inositol 1,4,5-Trifosfato/antagonistas & inhibidores , Receptores de Inositol 1,4,5-Trifosfato/genética , Leucemia Linfocítica Crónica de Células B/metabolismo , Leucemia Linfocítica Crónica de Células B/patología , Péptidos/química , Unión Proteica , Isoformas de Proteínas/metabolismo , Estructura Terciaria de Proteína , Proteínas Proto-Oncogénicas c-bcl-2/química , Interferencia de ARN , ARN Interferente Pequeño/metabolismoRESUMEN
Hyperosmotic stress promotes rapid and pronounced apoptosis in cultured cardiomyocytes. Here, we investigated if Ca(2+) signals contribute to this response. Exposure of cardiomyocytes to sorbitol [600 mosmol (kg water)(-1)] elicited large and oscillatory intracellular Ca(2+) concentration increases. These Ca(2+) signals were inhibited by nifedipine, Cd(2+), U73122, xestospongin C and ryanodine, suggesting contributions from both Ca(2+) influx through voltage dependent L-type Ca(2+) channels plus Ca(2+) release from intracellular stores mediated by IP(3) receptors and ryanodine receptors. Hyperosmotic stress also increased mitochondrial Ca(2+) levels, promoted mitochondrial depolarization, reduced intracellular ATP content, and activated the transcriptional factor cyclic AMP responsive element binding protein (CREB), determined by increased CREB phosphorylation and electrophoretic mobility shift assays. Incubation with 1 mM EGTA to decrease extracellular [Ca(2+)] prevented cardiomyocyte apoptosis induced by hyperosmotic stress, while overexpression of an adenoviral dominant negative form of CREB abolished the cardioprotection provided by 1 mM EGTA. These results suggest that hyperosmotic stress induced by sorbitol, by increasing Ca(2+) influx and raising intracellular Ca(2+) concentration, activates Ca(2+) release from stores and causes cell death through mitochondrial function collapse. In addition, the present results suggest that the Ca(2+) increase induced by hyperosmotic stress promotes cell survival by recruiting CREB-mediated signaling. Thus, the fate of cardiomyocytes under hyperosmotic stress will depend on the balance between Ca(2+)-induced survival and death pathways.
Asunto(s)
Apoptosis/fisiología , Señalización del Calcio/fisiología , Calcio/metabolismo , Supervivencia Celular , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/fisiología , Sorbitol/farmacología , Animales , Células Cultivadas , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Humanos , Indicadores y Reactivos/farmacología , Mitocondrias/metabolismo , Miocitos Cardíacos/citología , Presión Osmótica , Ratas , Ratas Sprague-DawleyRESUMEN
BACKGROUND AND PURPOSE: Venoms are a rich source of ligands for ion channels, but very little is known about their capacity to modulate G-protein coupled receptor (GPCR) activity. We developed a strategy to identify novel toxins targeting GPCRs. EXPERIMENTAL APPROACH: We studied the interactions of mamba venom fractions with alpha(1)-adrenoceptors in binding experiments with (3)H-prazosin. The active peptide (AdTx1) was sequenced by Edman degradation and mass spectrometry fragmentation. Its synthetic homologue was pharmacologically characterized by binding experiments using cloned receptors and by functional experiments on rabbit isolated prostatic smooth muscle. KEY RESULTS: AdTx1, a 65 amino-acid peptide stabilized by four disulphide bridges, belongs to the three-finger-fold peptide family. It has subnanomolar affinity (K(i)= 0.35 nM) and high specificity for the human alpha(1A)-adrenoceptor subtype. We showed high selectivity and affinity (K(d)= 0.6 nM) of radio-labelled AdTx1 in direct binding experiments and revealed a slow association constant (k(on)= 6 x 10(6).M(-1).min(-1)) with an unusually stable alpha(1A)-adrenoceptor/AdTx1 complex (t(1/2diss)= 3.6 h). AdTx1 displayed potent insurmountable antagonism of phenylephrine's actions in vitro (rabbit isolated prostatic muscle) at concentrations of 10 to 100 nM. CONCLUSIONS AND IMPLICATIONS: AdTx1 is the most specific and selective peptide inhibitor for the alpha(1A)-adrenoceptor identified to date. It displays insurmountable antagonism, acting as a potent relaxant of smooth muscle. Its peptidic nature can be exploited to develop new tools, as a radio-labelled-AdTx1 or a fluoro-labelled-AdTx1. Identification of AdTx1 thus offers new perspectives for developing new drugs for treating benign prostatic hyperplasia.
Asunto(s)
Antagonistas de Receptores Adrenérgicos alfa 1 , Venenos Elapídicos/química , Elapidae , Péptidos/farmacología , Secuencia de Aminoácidos , Animales , Fraccionamiento Químico , Venenos Elapídicos/aislamiento & purificación , Venenos Elapídicos/farmacología , Humanos , Técnicas In Vitro , Masculino , Espectrometría de Masas , Datos de Secuencia Molecular , Contracción Muscular/efectos de los fármacos , Músculo Liso/efectos de los fármacos , Músculo Liso/fisiología , Péptidos/aislamiento & purificación , Pichia , Próstata/efectos de los fármacos , Próstata/fisiología , Conejos , Ensayo de Unión Radioligante , Ratas , Ratas Sprague-Dawley , Receptores Adrenérgicos alfa 1RESUMEN
The skeletal neuromuscular junction has been considered as a model of chemical synapses due to its relatively simple organization. It is made up of three cellular partners including the motoneuron nerve terminals, the peri-synaptic Schwann cells and a specialized region of skeletal muscle fibers. It has been extensively studied revealing its ultrastructural complexity involving many molecular actors. The neuromuscular junction is a highly specialized structure, optimized for the rapid transmission of information from the presynaptic nerve terminal to the post-synaptic muscle fiber. This rapid transmission requires a very close apposition of plasmic membranes of pre- and post-synaptic partners, and a strict structural and molecular arrangement on both sides of the narrow synaptic cleft separating nerve terminal and muscle membranes. In this short review, we summarize the knowledge regarding pre- and post-synaptic ultrastructural specializations and give an overview of some functional aspects of neuromuscular transmission, including the quantal acetylcholine release process, which will help to better understand the pharmacological actions of botulinum toxins in esthetic and corrective dermatology.
Asunto(s)
Músculo Esquelético/anatomía & histología , Músculo Esquelético/fisiología , Unión Neuromuscular/anatomía & histología , Unión Neuromuscular/fisiología , Transmisión Sináptica/fisiología , Acetilcolina/metabolismo , Humanos , Placa Motora/anatomía & histología , Placa Motora/fisiología , Neuronas Motoras/fisiología , Fibras Musculares Esqueléticas/fisiología , Neurotransmisores/metabolismo , Terminales Presinápticos/fisiología , Receptores Colinérgicos/fisiología , Sinapsis/fisiologíaRESUMEN
Several bacteria of the Clostridium genus (C. botulinum) produce 150 kDa di-chainal protein toxins referred as botulinum neurotoxins or BoNTs. They associate with non-toxic companion proteins and form a complex termed botulinum toxin. BoNTs specifically inhibit vesicular neurotransmitter release. The cellular action of BoNTs can be depicted according to a multi-step model : The toxin's heavy chain mediates binding to specific receptors comprised of a ganglioside moiety and a vesicular protein (SV2 for BoNT type A, synaptotagmin for BoNT type B), followed by endocytotic internalisation of the BoNT/receptor complex. Vesicle recycling induces BoNT internalisation. Upon acidification of vesicles, the light chain of the neurotoxin is translocated into the cytosol. Here, this zinc-endopeptidase cleaves one or two among three synaptic proteins (VAMP-synapto-brevin, SNAP25, and syntaxin). As the three protein targets of BoNT play major role in fusion of synaptic vesicles at the release sites, their cleavage is followed by blockade of neurotransmitter exocytosis. Importantly, as the BoNT receptors and intracellular targets are present in all nerve terminals, the BoNTs are not specific for cholinergic transmission. Duration of their inhibitory action is mainly determined by the the life-time of the toxin's light chain in the cytosol. Sprouting of new nerve-endings, which are retracted when the poisoned nerve terminals have recovered full functionality, may lead to anticipated recovery of the poisoned nerve terminals.
Asunto(s)
Toxinas Botulínicas/farmacología , Fármacos Dermatológicos/farmacología , Fármacos Neuromusculares/farmacología , Toxinas Botulínicas/química , Toxinas Botulínicas/metabolismo , Clostridium botulinum/metabolismo , Fármacos Dermatológicos/química , Fármacos Dermatológicos/metabolismo , Humanos , Metaloendopeptidasas/metabolismo , Datos de Secuencia Molecular , Proteínas del Tejido Nervioso/metabolismo , Fármacos Neuromusculares/química , Fármacos Neuromusculares/metabolismo , Transmisión Sináptica/efectos de los fármacosRESUMEN
The inositol 1,4,5-trisphosphate receptor (IP(3)R) is a major regulator of apoptotic signaling. Through interactions with members of the Bcl-2 family of proteins, it drives calcium (Ca(2+)) transients from the endoplasmic reticulum (ER) to mitochondria, thereby establishing a functional and physical link between these organelles. Importantly, the IP(3)R also regulates autophagy, and in particular, its inhibition/depletion strongly induces macroautophagy. Here, we show that the IP(3)R antagonist xestospongin B induces autophagy by disrupting a molecular complex formed by the IP(3)R and Beclin 1, an interaction that is increased or inhibited by overexpression or knockdown of Bcl-2, respectively. An effect of Beclin 1 on Ca(2+) homeostasis was discarded as siRNA-mediated knockdown of Beclin 1 did not affect cytosolic or luminal ER Ca(2+) levels. Xestospongin B- or starvation-induced autophagy was inhibited by overexpression of the IP(3)R ligand-binding domain, which coimmunoprecipitated with Beclin 1. These results identify IP(3)R as a new regulator of the Beclin 1 complex that may bridge signals converging on the ER and initial phagophore formation.
Asunto(s)
Proteínas Reguladoras de la Apoptosis/metabolismo , Autofagia/fisiología , Receptores de Inositol 1,4,5-Trifosfato/metabolismo , Proteínas de la Membrana/metabolismo , Proteínas Asociadas a Microtúbulos/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Animales , Proteínas Reguladoras de la Apoptosis/genética , Autofagia/efectos de los fármacos , Beclina-1 , Calcio/metabolismo , Línea Celular , Línea Celular Tumoral , Técnicas de Silenciamiento del Gen , Células HeLa , Humanos , Receptores de Inositol 1,4,5-Trifosfato/antagonistas & inhibidores , Compuestos Macrocíclicos/farmacología , Proteínas de la Membrana/genética , Oxazoles/farmacología , Proteínas Proto-Oncogénicas c-bcl-2/genética , ARN Interferente Pequeño/metabolismo , RatasRESUMEN
The reduction of intracellular 1,4,5-inositol trisphosphate (IP(3)) levels stimulates autophagy, whereas the enhancement of IP(3) levels inhibits autophagy induced by nutrient depletion. Here, we show that knockdown of the IP(3) receptor (IP(3)R) with small interfering RNAs and pharmacological IP(3)R blockade is a strong stimulus for the induction of autophagy. The IP(3)R is known to reside in the membranes of the endoplasmic reticulum (ER) as well as within ER-mitochondrial contact sites, and IP(3)R blockade triggered the autophagy of both ER and mitochondria, as exactly observed in starvation-induced autophagy. ER stressors such as tunicamycin and thapsigargin also induced autophagy of ER and, to less extent, of mitochondria. Autophagy triggered by starvation or IP(3)R blockade was inhibited by Bcl-2 and Bcl-X(L) specifically targeted to ER but not Bcl-2 or Bcl-X(L) proteins targeted to mitochondria. In contrast, ER stress-induced autophagy was not inhibited by Bcl-2 and Bcl-X(L). Autophagy promoted by IP(3)R inhibition could not be attributed to a modulation of steady-state Ca(2+) levels in the ER or in the cytosol, yet involved the obligate contribution of Beclin-1, autophagy-related gene (Atg)5, Atg10, Atg12 and hVps34. Altogether, these results strongly suggest that IP(3)R exerts a major role in the physiological control of autophagy.
Asunto(s)
Autofagia , Receptores de Inositol 1,4,5-Trifosfato/metabolismo , Animales , Autofagia/genética , Calcio/metabolismo , Retículo Endoplásmico/efectos de los fármacos , Retículo Endoplásmico/metabolismo , Privación de Alimentos , Células HeLa , Humanos , Inositol 1,4,5-Trifosfato/metabolismo , Receptores de Inositol 1,4,5-Trifosfato/antagonistas & inhibidores , Compuestos Macrocíclicos/farmacología , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Oxazoles/farmacología , Isoformas de Proteínas/metabolismo , Ratas , Proteína bcl-X/metabolismoRESUMEN
Acetylcholinesterase (AChE) plays an essential role in neuromuscular transmission, therefore it is surprising that AChE knockout (KO) mice could live to the adulthood. Neuromuscular functioning in KO and normal (wild type, WT) mice were studied, at different age (1.5-, 4- and 9-month-old). Hindlimb muscle force productions in response to nerve or muscle electric stimulation were recorded in situ and in vitro. Our results show that contrary to WT mice, 1.5-, 4- and 9-month-old KO mice exhibited a decreased in tetanic force during short periods (500 ms) of repetitive nerve stimulations (tetanic fade). Nevertheless submaximal muscle forces in response to single or repetitive nerve stimulation were increased (potentiation) in 1.5-, 4- and 9-month-old KO mice as compared to WT mice (p<0.05). Tetanic fade and potentiation were absent when muscles were directly stimulated, indicating neuromuscular transmission alterations in KO mice. Contrary to younger mice, muscle weight and maximal tetanic force in response to repetitive nerve stimulation were not reduced in 4- and 9-month-old KO mice as compared to WT mice (p>0.05). In conclusion AChE deficit leads to marked neuromuscular alterations in hind limb muscle functioning and a prominent symptom is the lack of resistance to fatigue.
Asunto(s)
Acetilcolina/metabolismo , Acetilcolinesterasa/genética , Enfermedades de la Unión Neuromuscular/enzimología , Unión Neuromuscular/enzimología , Transmisión Sináptica/genética , Animales , Modelos Animales de Enfermedad , Estimulación Eléctrica , Femenino , Miembro Posterior/inervación , Miembro Posterior/fisiopatología , Masculino , Ratones , Ratones Noqueados , Contracción Muscular/genética , Fatiga Muscular/genética , Debilidad Muscular/enzimología , Debilidad Muscular/genética , Debilidad Muscular/fisiopatología , Músculo Esquelético/inervación , Músculo Esquelético/fisiopatología , Síndromes Miasténicos Congénitos/enzimología , Síndromes Miasténicos Congénitos/genética , Síndromes Miasténicos Congénitos/fisiopatología , Unión Neuromuscular/genética , Unión Neuromuscular/fisiopatología , Enfermedades de la Unión Neuromuscular/genética , Enfermedades de la Unión Neuromuscular/fisiopatología , Tamaño de los Órganos/genéticaRESUMEN
A new specific voltage-sensitive calcium channel (VSCC) blocker has been isolated from the venom of the fish-hunting cone snail Conus consors. This peptide, named omega-Ctx CNVIIA, consists of 27 amino acid residues folded by 3 disulfide bridges. Interestingly, loop 4, which is supposed to be crucial for selectivity, shows an unusual sequence (SSSKGR). The synthesis of the linear peptide was performed using the Fmoc strategy, and the correct folding was achieved in the presence of guanidinium chloride, potassium buffer, and reduced/oxidized glutathione at 4 degrees C for 3 days. Both synthetic and native toxin caused an intense shaking activity, characteristic of omega-conotoxins targeting N-type VSCC when injected intracerebroventricularly to mice. Binding studies on rat brain synaptosomes revealed that the radioiodinated omega-Ctx CNVIIA specifically and reversibly binds to high-affinity sites with a K(d) of 36.3 pM. Its binding is competitive with omega-Ctx MVIIA at low concentration (K(i) = 2 pM). Moreover, omega-Ctx CNVIIA exhibits a clear selectivity for N-type VSCCs versus P/Q-type VSCCs targeted respectively by radioiodinated omega-Ctx GVIA and omega-Ctx MVIIC. Although omega-Ctx CNVIIA clearly blocked N-type Ca(2+) current in chromaffin cells, this toxin did not inhibit acetylcholine release evoked by nerve stimuli at the frog neuromuscular junction, in marked contrast to omega-Ctx GVIA. omega-Ctx CNVIIA thus represents a new selective tool for blocking N-type VSCC that displays a unique pharmacological profile and highlights the diversity of voltage-sensitive Ca(2+) channels in the animal kingdom.
Asunto(s)
Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio Tipo N/efectos de los fármacos , omega-Conotoxinas/farmacología , Secuencia de Aminoácidos , Animales , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Bloqueadores de los Canales de Calcio/síntesis química , Bloqueadores de los Canales de Calcio/aislamiento & purificación , Canales de Calcio Tipo N/metabolismo , Células Cromafines/fisiología , Ciprinodontiformes , Relación Dosis-Respuesta a Droga , Inyecciones Intraventriculares , Masculino , Potenciales de la Membrana/fisiología , Ratones , Datos de Secuencia Molecular , Músculo Liso/fisiología , Técnicas de Placa-Clamp , Unión Proteica , Ensayo de Unión Radioligante , Rana esculenta , Ratas , Homología de Secuencia de Aminoácido , Caracoles/química , Relación Estructura-Actividad , omega-Conotoxinas/síntesis química , omega-Conotoxinas/aislamiento & purificaciónRESUMEN
Two new quaternary indole alkaloids 5',6'-dehydroguiachrysine (1) and 5',6'-dehydroguiaflavine (2) were isolated from Strychnos guianensis stem bark. Their structures were determined by analysis of spectral data. Their inhibitory effects on neuromuscular transmission are also reported and compared to that of other quaternary alkaloids.
Asunto(s)
Alcaloides/aislamiento & purificación , Indoles/aislamiento & purificación , Loganiaceae/química , Tallos de la Planta/química , Alcaloides/química , Indoles/química , Estructura Molecular , Análisis EspectralRESUMEN
Scorpion alpha-toxins from Leiurus quinquestriatus hebraeus, LqhII and LqhIII, are similarly toxic to mice when administered by a subcutaneous route, but in mouse brain LqhII is 25-fold more toxic. Examination of the two toxins effects in central nervous system (CNS), peripheral preparations and expressed sodium channels revealed the basis for their differential toxicity. In rat brain synaptosomes, LqhII binds with high affinity, whereas LqhIII competes only at high concentration for LqhII-binding sites in a voltage-dependent manner. LqhII strongly inhibits sodium current inactivation of brain rBII subtype expressed in HEK293 cells, whereas LqhIII is weakly active at 2 microM, suggesting that LqhIII affects sodium channel subtypes other than rBII in the brain. In the periphery, both toxins inhibit tetrodotoxin-sensitive sodium current inactivation in dorsal root ganglion neurons, and are strongly active directly on the muscle and on expressed muI channels. Only LqhII, however, induced repetitive end-plate potentials in mouse phrenic nerve-hemidiaphragm muscle preparation by direct effect on the motor nerve. Thus, rBII and sodium channel subtypes expressed in peripheral nervous system (PNS) serve as the main targets for LqhII but are mostly not sensitive to LqhIII. Toxicity of both toxins in periphery may be attributed to the direct effect on muscle. Our data elucidate, for the first time, how different toxins affect mammalian central and peripheral excitable cells, and reveal unexpected subtype specificity of toxins that interact with receptor site 3.
Asunto(s)
Química Encefálica/fisiología , Activación del Canal Iónico/efectos de los fármacos , Nervio Frénico/química , Venenos de Escorpión/farmacología , Canales de Sodio/metabolismo , Animales , Sitios de Unión/fisiología , Encéfalo/citología , Células Cultivadas , Femenino , Ganglios Espinales/química , Ganglios Espinales/citología , Humanos , Activación del Canal Iónico/fisiología , Riñón/citología , Mamíferos , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Ratones , Datos de Secuencia Molecular , Neuronas Motoras/química , Neuronas Motoras/citología , Neuronas Motoras/efectos de los fármacos , Contracción Muscular/efectos de los fármacos , Contracción Muscular/fisiología , Músculo Esquelético/química , Músculo Esquelético/citología , Unión Neuromuscular/química , Unión Neuromuscular/citología , Neuronas Aferentes/química , Neuronas Aferentes/citología , Neuronas Aferentes/efectos de los fármacos , Técnicas de Placa-Clamp , Nervio Frénico/citología , Ratas , Ratas Sprague-Dawley , Ratas Wistar , Venenos de Escorpión/metabolismo , Homología de Secuencia de Aminoácido , Canales de Sodio/química , Sinaptosomas/química , Sinaptosomas/efectos de los fármacos , Sinaptosomas/fisiologíaRESUMEN
SPOCK is prevalent in developing synaptic fields of the central nervous system (Charbonnier et al., 2000. Mech. Dev. 90, 317-321). The expression of SPOCK during neuromuscular junction (NMJ) formation was compared to agrin and acetylcholine receptor (AChR) distribution. SPOCK is detected within the myogenic masses during the early steps of embryonic development, and distributed in the cytoplasm of myotubes before coclustering with AChRs. In the adult, SPOCK is present in axons and is highly expressed by Schwann cells. SPOCK altered expression pattern after nerve lesioning, or cholinergic transmission blockade, strongly indicate that its cellular distribution at the NMJ depends on innervation.
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Músculo Esquelético/embriología , Unión Neuromuscular/embriología , Unión Neuromuscular/crecimiento & desarrollo , Proteoglicanos/genética , Proteoglicanos/metabolismo , Animales , Citoplasma/metabolismo , Regulación del Desarrollo de la Expresión Génica , Ratones , Ratones Endogámicos , Fibras Musculares Esqueléticas/fisiología , Proteoglicanos/inmunología , Ratas , Ratas Sprague-Dawley , Receptores Colinérgicos/metabolismoAsunto(s)
Toxinas Botulínicas/toxicidad , Células Cromafines/efectos de los fármacos , Unión Neuromuscular/efectos de los fármacos , Toxina Tetánica/toxicidad , Animales , Toxinas Botulínicas/farmacología , Clostridium botulinum/metabolismo , Clostridium tetani/metabolismo , Ratones , Neuronas/efectos de los fármacos , Células PC12/efectos de los fármacos , Ratas , Toxina Tetánica/farmacologíaRESUMEN
Two new quaternary alkaloids, 9-methoxy-Nb-methylgeissoschizol and guiachrysine together with the known compounds C-alkaloid O, fluorocurine, mavacurine, macusine B and C-profluorocurine, were isolated from Strychnos guianensis stembark. The structures of the compounds were elucidated on the basis of spectroscopic studies.
Asunto(s)
Alcaloides/aislamiento & purificación , Indoles/aislamiento & purificación , Fármacos Neuromusculares/aislamiento & purificación , Plantas Medicinales/química , Alcaloides/química , Alcaloides/toxicidad , Animales , Técnicas In Vitro , Indoles/química , Indoles/toxicidad , Potenciales de la Membrana/efectos de los fármacos , Ratones , Fármacos Neuromusculares/química , Fármacos Neuromusculares/toxicidad , Unión Neuromuscular/efectos de los fármacos , Unión Neuromuscular/fisiología , Tallos de la Planta/química , Ranidae , América del SurRESUMEN
Equinatoxin-II (EqTx-II), a cytotoxic protein (mol.wt 20 kDa) isolated from the sea anemone Actinia equina, was found to consistently increase the three-dimensional projected area of differentiated neuroblastoma (NG108-15) cells provided Ca(2+) was present in the medium. No swelling was detected when external NaCl was replaced by sucrose, but replacement of NaCl by Na-isethionate did not prevent the swelling, as revealed by confocal laser scanning microscopy. In addition, microspectrofluorometric measurements in cells preloaded with the Ca(2+) indicator fura-2/AM revealed that EqTx-II (100 nM) markedly increased the fluorescence (F(340)/F(380)) ratio indicating a rise of intracellular Ca(2+) concentration ([Ca(2+)](i)). The elevation of [Ca(2+)](i) exhibited two components that seem to be related to the kinetics of EqTx-II-induced Ca(2+) entry since pretreatment of cells with Ca(2+)-ATPase inhibitors (thapsigargin), Ca(2+) channel blockers (nifedipine and Gd(3+)) or prolonged exposure to a high K(+) (75 mM) medium did not alter EqTx-II-induced Ca(2+) signals. As far as we know, this is the first demonstration that EqTx-II causes swelling of neuroblastoma cells and that this effect is correlated both with an increase of [Ca(2+)](i) and needs the presence of extracellular Na(+). It is suggested that EqTx-II has the ability to insert into the plasma membrane of neuroblastoma cells and to form pores altering the membrane permeability and the intracellular osmolality, inducing a marked influx of water into the cells.
Asunto(s)
Calcio/metabolismo , Venenos de Cnidarios/toxicidad , Citotoxinas/toxicidad , Neuroblastoma/patología , Sodio/metabolismo , Animales , Bloqueadores de los Canales de Calcio/farmacología , Señalización del Calcio/efectos de los fármacos , ATPasas Transportadoras de Calcio/antagonistas & inhibidores , Diferenciación Celular , Inhibidores Enzimáticos/farmacología , Potenciales de la Membrana/efectos de los fármacos , Ratones , Microscopía Confocal/métodos , Ratas , Anémonas de Mar/química , Células Tumorales CultivadasRESUMEN
The effects of trachynilysin (TLY), a protein toxin isolated from stonefish (Synanceia trachynis) venom, were studied on the electrical and mechanical activities of frog atrial fibres. TLY (1 microg/ml) hyperpolarized the membrane, shortened the action potential (AP) duration (APD), exerted a negative inotropic effect and elicited contracture. These effects did not develop in the presence of atropine. TLY shortened the APD of fibres isolated from a frog completely paralyzed with botulinum type A toxin, in the presence of Ca2+ but not when Ca2+ was replaced by Sr2+. TLY increased the basal and the peak of the fluorescence ratio of stimulated fibres loaded with fura-2. Confocal laser scanning microscopy revealed the existence of a diffuse innervation in atrial tissue. Our results suggest that TLY enhances the release of acetylcholine from atrial cholinergic nerve terminals and activates indirectly muscarinic receptors leading to a shortening of APD. They also show that the mechanical effects induced by TLY are due to an increase of the Ca2+ influx and to a rise in intracellular Ca2+ levels which leads to (i) a slowing of the Na+/Ca2+ exchange activity, which accounts for the contracture and (ii) the activation of a Ca2+-dependent K+ current involved in the APD shortening.
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Venenos de los Peces/farmacología , Contracción Miocárdica/efectos de los fármacos , Neurotoxinas/farmacología , Acetilcolina/metabolismo , Potenciales de Acción/efectos de los fármacos , Animales , Atropina/farmacología , Calcio/metabolismo , Interacciones Farmacológicas , Estimulación Eléctrica , Peces , Atrios Cardíacos/efectos de los fármacos , Rana esculentaRESUMEN
Trachynilysin, a 159 kDa dimeric protein purified from stonefish (Synanceia trachynis) venom, dramatically increases spontaneous quantal transmitter release at the frog neuromuscular junction, depleting small clear synaptic vesicles, whilst not affecting large dense core vesicles. The basis of this insensitivity of large dense core vesicles exocytosis was examined using a fluorimetric assay to determine whether the toxin could elicit catecholamine release from bovine chromaffin cells. Unlike the case of the motor nerve endings, nanomolar concentrations of trachynilysin evoked sustained Soluble N-ethylmaleimide-sensitive fusion protein Attachment Protein REceptor-dependent exocytosis of large dense core vesicles, but only in the presence of extracellular Ca2+. However, this response to trachynilysin does not rely on Ca2+ influx through voltage-activated Ca2+ channels because the secretion was only slightly affected by blockers of L, N and P/Q types. Instead, trachynilysin elicited a localized increase in intracellular fluorescence monitored with fluo-3/AM, that precisely co-localized with the increase of fluorescence resulting from caffeine-induced release of Ca2+ from intracellular stores. Moreover, depletion of the latter stores inhibited trachynilysin-induced exocytosis. Thus, the observed requirement of external Ca2+ for stimulation of large dense core vesicles exocytosis from chromaffin cells implicates plasma membrane channels that signal efflux of Ca2+ from intracellular stores. This study also suggests that the bases of exocytosis of large dense core vesicles from motor nerve terminals and neuroendocrine cells are distinct.
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Calcio/fisiología , Catecolaminas/metabolismo , Células Cromafines/efectos de los fármacos , Células Cromafines/metabolismo , Venenos de los Peces/farmacología , Proteínas de la Membrana/fisiología , Proteínas de Transporte Vesicular , Animales , Transporte Biológico , Calcio/metabolismo , Bovinos , Células Cultivadas , Gránulos Citoplasmáticos/fisiología , Exocitosis/efectos de los fármacos , Neurotoxinas/farmacología , Proteínas SNARERESUMEN
Equinatoxin II (EqT II) is a basic 20 kD protein isolated from the sea anemone Actinia equina. Intravenous injection of 3 LD50 of EqT II causes cardiorespiratory arrest. The aim of our study was to check the effects of EqT II on neuronal cells to assess the role of neuronal mechanisms in respiratory arrest after intravenous injection of the toxin. Effects of EqT II on mouse neuroblastoma x rat glioma NG108-15 cell were studied using confocal laser scanning microscopy and by Fura-2 fluorescence measurements. The results show that EqT II applied in nanomolar range increases intracellular Ca2+ activity significantly, which is possibly responsible for the morphological changes of NG108-15 cells after the exposure to 10 nM EqT II. Intracellular increase in Ca2+ activity can not be prevented by use of the various pharmacological substances (e.g. Ca2+ channels blocker Verapamil and Bekanamycin). Swelling of the NG108-15 cells after the exposure to the EqT II also can not be blocked with the sodium channel blocker tetrodotoxin. Increase in the intracellular Ca2+ activity is probably a result of Ca2+ entry through pores produced by the toxin, which has been shown by other authors on other cells and on phospholipid bilayer. Respiratory arrest after intravenous injection of the toxin can be caused by the action of the toxin on neuronal cells in medulla oblongata provided that EqT II can damage blood brain barrier thus enabling access to the neuronal cells. The results allow the conclusion that EqT II can affect normal calcium homeostasis and cell morphology of neuronal cells that can disturb cell physiology and its function thus affecting normal respiratory pattern.
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Calcio/metabolismo , Venenos de Cnidarios/farmacología , Membranas Intracelulares/metabolismo , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Animales , Ratones , Ratas , Células Tumorales CultivadasRESUMEN
1. Fluorescent dyes have been used at the frog neuromuscular junction to label synaptic vesicular membrane. Retrieved membrane is reformed into vesicles, which are released along with pre-existing vesicles. Consequently, if vesicular refilling with acetylcholine (ACh) is depressed by inhibitors, two sizes of quanta should be released: normal and smaller. As recycling continues the fraction of smaller size quanta should increase exponentially. 2. We enhanced the rate of quantal release by elevating the K+ concentration. The principal inhibitors were (-)-vesamicol (VES), hemicholinium-3 (HC3), and NH4+. Quantal size measurements were fitted to one and to two cumulative lognormal probability distribution functions. When two fitted better, the statistical significance assessment took into account the three additional parameters used in calculating the fit. 3. After recycling in the presence of inhibitor, many sets were fitted better by two lognormal functions. As recycling continued, the fraction of the miniature endplate potential voltage-time integrals ( MEPPs) in the larger sub-population decreased exponentially. 4. The size of the releasable pool was estimated by counting the quanta released by carbonyl cyanide m-chlorophenylhydrazone (CCCP). This was compared to pool sizes calculated from the inhibitor experiments. The two estimates of pool size were indistinguishable, with mean values ranging from about 170,000 to 270,000. 5. With all of the treatments tested, the means of the sizes in the smaller sub-population of MEPPs were about 1/3 those of the larger sub-populations. 6. Recycling synaptic vesicles appear to be incorporated into the releasable pool from which they have roughly the same probability of release as the pre-existing vesicles.
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Macrólidos , Unión Neuromuscular/metabolismo , Neurotransmisores/metabolismo , ATPasas de Translocación de Protón Vacuolares , Animales , Antibacterianos/farmacología , Carbonil Cianuro m-Clorofenil Hidrazona/farmacología , Concanavalina A/farmacología , Electrofisiología , Inhibidores Enzimáticos/farmacología , Predicción , Placa Motora/fisiología , Unión Neuromuscular/efectos de los fármacos , Unión Neuromuscular/fisiología , Potasio/farmacología , ATPasas de Translocación de Protón/antagonistas & inhibidores , Rana pipiens , SolucionesRESUMEN
The effects of hyperosmolar D-mannitol were studied on single frog myelinated nerve fibres previously poisoned with Caribbean ciguatoxin-1 (C-CTX-1), a new toxin isolated from the pelagic fish Caranx latus inhabiting the Caribbean region. In current-clamped myelinated axons, C-CTX-1 (50-120 nM) caused spontaneous and repetitive action potential discharges after a short delay. In addition, the toxin produced a marked swelling of nodes of Ranvier of myelinated axons that reached a steady state within about 90 min, as revealed by using confocal laser scanning microscopy. The increased excitability and the nodal swelling caused by C-CTX-1 were prevented or reversed by an external hyperosmotic solution containing 100 mM D-mannitol. Moreover, the C-CTX-1-induced nodal swelling was completely prevented by the blockade of voltage-sensitive sodium channels by tetrodotoxin (TTX). It is suggested that C-CTX-1, by increasing nerve membrane excitability, enhances Na(+) entry into nodes of Ranvier through TTX-sensitive sodium channels, which directly or indirectly disturb the osmotic equilibrium between intra- and extra-axonal media resulting in an influx of water that was responsible for the long-lasting nodal swelling. The fact, that hyperosmolar D-mannitol either reversed or prevented the neurocellular actions of C-CTX-1, is of particular interest since it provides the rational basis for its use to treat the neurological symptoms of ciguatera fish poisoning in the Caribbean area.