Optimization of multi-environment trials for genomic selection based on crop models.

KEY MESSAGE: We propose a statistical criterion to optimize multi-environment trials to predict genotype × environment interactions more efficiently, by combining crop growth models and genomic selection models. Genotype × environment interactions (GEI) are common in plant multi-environment trials (METs). In this context, models developed for genomic selection (GS) that refers to the use of genome-wide information for predicting breeding values of selection candidates need to be adapted. One promising way to increase prediction accuracy in various environments is to combine ecophysiological and genetic modelling thanks to crop growth models (CGM) incorporating genetic parameters. The efficiency of this approach relies on the quality of the parameter estimates, which depends on the environments composing this MET used for calibration. The objective of this study was to determine a method to optimize the set of environments composing the MET for estimating genetic parameters in this context. A criterion called OptiMET was defined to this aim, and was evaluated on simulated and real data, with the example of wheat phenology. The MET defined with OptiMET allowed estimating the genetic parameters with lower error, leading to higher QTL detection power and higher prediction accuracies. MET defined with OptiMET was on average more efficient than random MET composed of twice as many environments, in terms of quality of the parameter estimates. OptiMET is thus a valuable tool to determine optimal experimental conditions to best exploit MET and the phenotyping tools that are currently developed.

Maximizing the reliability of genomic selection by optimizing the calibration set of reference individuals: comparison of methods in two diverse groups of maize inbreds (Zea mays L.).

Genomic selection refers to the use of genotypic information for predicting breeding values of selection candidates. A prediction formula is calibrated with the genotypes and phenotypes of reference individuals constituting the calibration set. The size and the composition of this set are essential parameters affecting the prediction reliabilities. The objective of this study was to maximize reliabilities by optimizing the calibration set. Different criteria based on the diversity or on the prediction error variance (PEV) derived from the realized additive relationship matrix-best linear unbiased predictions model (RA-BLUP) were used to select the reference individuals. For the latter, we considered the mean of the PEV of the contrasts between each selection candidate and the mean of the population (PEVmean) and the mean of the expected reliabilities of the same contrasts (CDmean). These criteria were tested with phenotypic data collected on two diversity panels of maize (Zea mays L.) genotyped with a 50k SNPs array. In the two panels, samples chosen based on CDmean gave higher reliabilities than random samples for various calibration set sizes. CDmean also appeared superior to PEVmean, which can be explained by the fact that it takes into account the reduction of variance due to the relatedness between individuals. Selected samples were close to optimality for a wide range of trait heritabilities, which suggests that the strategy presented here can efficiently sample subsets in panels of inbred lines. A script to optimize reference samples based on CDmean is available on request.

Modelling gene flow between oilseed rape and wild radish. I. Evolution of chromosome structure.

The assessment of gene flow from crop species to weeds has found a new emphasis over the last years because of the marketing of transgenic crops and the possible selective advantage that crop (trans)gene may confer to the weeds. Several studies focused on the F1 interspecific hybrid production but few data are available on the factors affecting the genetic structure of advanced generations. It depends on the genomic structure of the species concerned as well as on the degree of their genome homology that affect the occurrence of intergenomic recombination. Oilseed rape (Brassica napus, AACC, 2n = 38)-wild radish (Raphanus raphanistrum, RrRr, 2n = 18), a distantly related weed, is a good model to address such questions. From seven male sterile oilseed rape lines carrying an herbicide tolerance transgene, F1 interspecific hybrids and four advanced generations were produced under field conditions with wild radish as pollinator. Observation of hybrid chromosome numbers across four generations revealed a high variability, especially in the "BC1" generation. A regression model was fitted in order to describe the relationship between parent and offspring chromosome numbers. The effects of generation, transgenic line and selection pressure on the mean relationship were investigated. The first two factors had an influence on the rate of decrease of chromosome numbers, whereas selection pressure resulted in the presence of an additional chromosome in the herbicide treated plants. The model provided a convenient framework for analysing how chromosome numbers evolve over successive hybridization events and it may prove useful as a basis for simulation-based approaches.

Maximal voluntary force and rate of force development in humans--importance of instruction.

The present investigation has been designed to confirm the effect of instruction (hard-and-fast instruction compared to fast instruction) upon maximal voluntary isometric force (MVF) and maximal rate of force development (MRFD) in muscle groups which differ with regards to muscle mass and usage. In addition, we took advantage of the force data collected during unilateral and bilateral leg extension, to compare the instruction effects on the indices of the bilateral deficits (BI, the differences between the data collected during bilateral extensions and the sum of the data collected during unilateral left and right extensions) with regard to MVF (BIMVF) and MRFD (BIMRFD). Force-time curves were recorded during maximal isometric contractions of the elbow flexors, the leg extensors of the take-off and lead legs and during bilateral leg extension in 26 healthy young male volunteers from the track-and-field national team of Tunisia. In the first protocol, the subjects were instructed to produce MFV as hard-and-fast as possible (instruction I). In the second protocol (instruction II) the subjects were instructed to provide MFRD, that is the most explosive force, by concentrating on the fastest contraction without concern for achieving maximal force. The present study confirmed the importance of an appropriate instruction for the measurement of MRDF The MRFD (F = 40.8, P < 0.001) were significantly higher when measured after instruction II compared to instruction I. The effect of the instruction upon MRFD were similar for muscle groups with different volumes, cortical representations and uses. The same results (F = 52.1; P < 0.001) were observed when MRFD was related to MVF [MRFD% = (MRFD/MVF) x 100]. On the other hand, MVF was similar following both instructions (ANOVA, F = 0.562; P = 0.454). Moreover, the results of the present study suggested that the effect of instruction was significantly larger for BIMRFD than for BIMVF.

Effects of external loading on power output in a squat jump on a force platform: a comparison between strength and power athletes and sedentary individuals.

The aim of this study was to determine the effects of external loading on power output during a squat jump on a force platform in athletes specializing in strength and power events (6 elite weight-lifters and 16 volleyball players) and in 20 sedentary individuals. Instantaneous power was computed from time-force curves during vertical jumps with and without an external load (0, 5 or 10 kg worn in a special vest). The jumps were performed from a squat position, without lower limb counter-movement or an arm swing. Peak instantaneous power corresponded to the highest value of instantaneous power during jumping. Average power throughout the push phase of the jump was also calculated. A two-way analysis of variance showed significant interactions between the load and group effects for peak instantaneous power (P< 0.01) and average power (P< 0.001). Peak instantaneous power decreased significantly in sedentary individuals when moderate external loads were added. The peak instantaneous power at 0 kg was greater than that at 5 and 10 kg in the sedentary individuals. In contrast, peak instantaneous power was independent of load in the strength and power athletes. Mean power at 0 kg was significantly lower than at 5 kg in the athletes; at 0 kg it was significantly higher than at 10 kg in the sedentary males and at 5 and 10 kg in the sedentary females. In all groups, the force corresponding to peak instantaneous power increased and the velocity corresponding to peak instantaneous power decreased with external loading. The present results suggest that the effects of external loading on peak instantaneous power are not significant in strength and power athletes provided that the loads do not prevent peak velocity from being higher than the velocity that is optimal for maximal power output.

Optimal complete block designs to adjust for interplot competition with a covariance analysis.

Optimal complete block designs are given for variety trials with interplot competition. In the paper, competition is modeled using height differences between neighboring varieties. When variety heights are known at the design stage, a simulated annealing algorithm is proposed. The optimal designs tend to reduce height differences between neighboring varieties. When variety heights are not known in advance, neighbor-balanced designs at distances 1 and 2 are shown to have optimality properties.

The contribution of vision in dynamic spontaneous sways of male classical dancers according to student or professional level.

We investigated the involvement of vision in the regulation of dynamic equilibrium in male children and young adults performing a physical activity requiring a high level of spatial skill: self-induced body sways of ballet dancers on a free unstable platform, 45 professional male dancers (Paris Opera) participated in the study. They included two student groups (beginners and confirmed) and two performer groups (adolescent and adult). They maintained their equilibrium on the platform under different visual and position conditions. The displacement of the seesaw platform were calculated from accelerometer measures. Fast Fourier transform processing of stabilograms allowed spectral frequency analysis. The total spectrum energy and the energies of the three frequency bands (0-0.5 Hz, 0.5-2 Hz, 2-20 Hz) were determined. For all groups, ANOVA indicated that values were higher for eyes-closed than for eyes-open conditions. The visual dependence differed according to age: for 14-year-old students the postural control for dynamic equilibrium was less visually dependent than for 11-year-old students. The 18-year-old dancers, although professional, were more dependent on vision than 14-year-old student dancers. These 18-year-old dancers were still adolescent because they had recently undergone growth acceleration which could disturb their proprioceptive references and internal body representations. Thus, visual input may dominate over the other sensory inputs in the regulation of postural control.

A new rating scale of perceived exertion based on subjective estimation of exhaustion time: a preliminary study.

Perceived exertion has been investigated during incremental exercise to exhaustion with athletes, according to the 6-20 scale proposed by Borg (RPE, 1970) and a new scale based on the estimation of exhaustion time (t(lim)) (Estimated Time Limit or ETL, from 1 to 20; ETL= 21 - 2 n, with n = log2t(lim)). ETL increased linearly with the percentage of maximal aerobic power (%MAP) up to the ventilatory threshold (VT). Beyond VT, an inflection of the ETL-%MAP relationship was observed. RPE and ETL calculated at VT were equal to 15 +/- 1.7, i.e. an exercise intensity perceived as "hard", and 10 +/- 2.3, i.e. an estimated exhaustion time equal approximately to 45 minutes. Standard deviations for RPE and ETL at VT were too large for an accurate estimation of this threshold. The results of the present study suggest that RPE and ETL should be used in addition to physiological data, but not replace them in the prescription of a training program.

Ratings of perceived exertion (RPE) during cycling exercises at constant power output.

The purpose of the present investigation was to study the overall rating of perceived exertion (RPEov) according to the 6-20 scale proposed by Borg (1970) and muscular RPE (RPEmu) in exercises at constant load. The relationship between RPE and heart rate for three different loads was studied during exhausting exercises in 10 participants. Whether the drift of RPE during a 20 min exercise at constant load could be an index of the endurance time during long-lasting exercises at constant load was also investigated. At 1-week intervals, the participants performed cycling exercises up to exhaustion at 60, 73, and 86% maximal aerobic power (MAP) measured during an incremental test. Heart rate, RPEov, RPEmu and exhaustion time (tlim) were measured. The upward shift of the HRmax-RPE regressions was significant between 86, 73 and 60% MAP (p < 0.001) for RPEov and RPEmu. This result suggests that the equation HR = 10 x RPE proposed by Borg (1973) for incremental exercise is not valid for long-lasting exercise at constant load until exhaustion because the heart rate corresponding to a given RPE depends on load and time. Mean RPE increased linearly with time up to exhaustion. Unexpectedly, the relationships between RPEmu or RPEov and percentage of exhaustion time were similar for exercises at 60 and 73% MAP although the exhaustion times were very different (79.40 +/- 30.64 min versus 36.19 +/- 15.99 min, respectively) (p < 0.001). Consequently, it is likely that RPE was a subjective estimation of the hardness of exercise rather than the intensity of exercise. The RPE pattern at the beginning of long-lasting exercises at constant load (60 and 73% MAP) cannot be considered as a sensitive predictor of the point of self-imposed exhaustion for individuals. Indeed, the errors in the estimation of exhaustion time from extrapolation of RPE at the beginning of exercise were very large. Moreover, at 60% MAP, a steady-state in RPE was observed during 20 min in five subjects whose tlim were not longer than tlim of the other subjects. In addition, the data of the present study indicate that RPEmu could be more useful than RPEov in cycling.

The isometric force that induces maximal surface muscle deoxygenation.

To determine the external force that induces maximal deoxygenation of brachioradialis muscle 32 trained male subjects maintained isometric contractions using the elbow flexor muscles up to the limit time (isotonic part of the isometric contraction, IIC) and beyond that time for 120 s (anisotonic part of the isometric contraction). During IIC each subject maintained relative forces of either 25% and 70% maximal voluntary contraction (MVC), 50% and 100% MVC, or 40% and 60% MVC. Muscle oxygenation was assessed using a near infrared spectroscope, and expressed as a percentage of the reference value (deltaO2rest) which was the difference between the minimal oxygenation obtained after 6 min of ischaemia at rest and the maximal reoxygenation following the release of the tourniquet. During IIC at 25% MVC, muscle oxygenation decreased to 17 (SEM 3)% deltaO2rest, then it levelled off [25 (SEM 1)% deltaO2rest]. After the point at which target force could not be maintained, reoxygenation was very weak. During IIC at 40%, 50%, 60%, and 70% MVC, the lowest muscle oxygenation values were obtained after 15-20 s of contraction and corresponded to -18 (SEM 6), -59 (SEM 12) -31 (SEM 6), and -29 (SEM 6)% deltaO2rest, respectively. For the contraction at 100% MVC, the lowest oxygenation [-19 (SEM 9)% deltaO2rest] was obtained while force was decreasing (69% MVC). During the anisotonic part of the isometric contractions, the greatest reoxygenation rate was obtained after 50% MVC IIC (P < 0.001). Our results showed that during isometric elbow flexions between 25% and 100% MVC, there was no linear relationship between external force and muscle oxygenation, and that the maximal deoxygenation of the brachioradialis muscle was obtained at 50% MVC.

Ratings of perceived exertion (RPE) as an index of aerobic endurance during local and general exercises.

The main purpose of this study was to assess the correlations of RPE with indicators of endurance (critical power), exhaustion time and the electromyographic activity of a relevant muscle during general and local exercises. Eight healthy subjects participated in the study. During the first session, Maximal Aerobic Power (MAP) was measured by means of an incremental cycling exercise on a Monark ergometer. At 1-week intervals the participants performed a general or local exhausting exercise, which consisted of a cycle exercise (60, 73, 86 or 100% MAP) and a knee extension exercise (lifting a load between 17.5 and 32.5 kg every 3 s), respectively. The critical powers corresponding to cycling (CPcycling) and knee extension (CPknee) were calculated from the slope of the linear relationship between exhaustion time (tlim) and work. Rate of perceived exertion, concerning the whole body (overall RPE [RPEov]), RPE concerning the exercising muscles (muscular RPE [RPEmu]) and exhaustion time were measured. During the last session, the integrated electromyogram of the right Vastus Lateralis (iEMG s-1) was measured during short (20 s) cycling and knee extension non-exhausting exercises, with the same electrode location, at the different power outputs used during the exhausting exercises. The relationships between RPEmu at the fifth minute and log tlim and between iEMG% (percentage of the iEMG s-1 corresponding to 100% MAP during cycling) and log tlim were similar for the cycling and knee extension exercises. The mean values of RPEmu at the fifth minute of exercise calculated for CPcycling and CPknee were almost equal (12.3 +/- 1.7 versus 12.2 +/- 0.9). Similarly, the mean values of iEMG% corresponding to CPcycling and CPknee were equal (78.44 versus 79.02%). These results suggest that RPEmu is related to aerobic endurance and that the possibility of sustaining a high percentage of maximal aerobic power during a general exercise is mainly related to local muscular factors.

Maximal power and force-velocity relationships during cycling and cranking exercises in volleyball players. Correlation with the vertical jump test.

BACKGROUND: The aim of this study was to propose a test battery adjusted to volleyball players and to study the links between dynamic (vertical jump, force-velocity relationships and maximal anaerobic power in cranking and cycling) and static (maximal voluntary force and rate of force development in isometric conditions) performances. METHODS: The relationships between braking force (F) and peak velocity (V) have been determined for cycling and cranking exercises in 18 male volleyball players of a district league. According to previous studies, these F-V relationships were assumed to be linear and were expressed as follows: V = V0(1-F/F0), where V0 should be an estimate of the maximal velocity at zero braking force whereas F0 is assumed to be a braking force corresponding to zero velocity. Maximal anaerobic power in cycling (Pmax leg) and cranking (Pmax arm) were calculated as equal to 0.25 V0F0. The same subjects performed a vertical jump test (VJ) and a strength test on an isometric leg press with the measurement of the unilateral isometric maximal voluntary force (MVF) and indices of rate of isometric force development (RFD): maximal rate of force development (MRFD) and the time from 25% to 50% of MVF (T25-50). RESULTS: Pmax leg (15.8 +/- 1.4 W.kg-1) and V0 arm (259.6 +/- 13.1 rpm) were high but similar to the results of elite athletes, previously collected with the same protocols and the same devices. VJ was significantly with F0 leg, Pmax leg and Pmax arm related to body mass. The performances of the dynamic tests were significantly correlated and especially the parameters (V0, F0, Pmax) of the force velocity tests in cycling were significantly correlated with the same parameters in cranking. The results of the isometric tests (MVF, MRFD) were not correlated with VJ, except T25-50 of the left leg. CONCLUSIONS: A vertical jump test and a force velocity test with the arms are proposed for a test battery in volleyball players.

Work-exhaustion time relationships and the critical power concept. A critical review.

The present review is focused on the physiological meanings of the critical power concept proposed by Scherrer in 1954 and its applications to general exercises such as running, cycling and swimming. Since the first studies on the critical power of local exercises, many studies have found that critical power is correlated with indices which are related to aerobic endurance such as maximal oxygen uptake, ventilatory threshold, OBLA or maximal lactate steady state. In fact, the relationship between exhaustion time t(lim) and the Work Wlim (or Distance Dlim) performed at exhaustion is not exactly linear and, consequently, the power-t(lim) equation is not a true hyperbola. The effect of the range of t(lim), used in the calculation of the slope of the Wlim-t(lim) relationship (called critical power) are discussed. When critical power is calculated from short supramaximal exercises, this power is higher than the power output which corresponds to a lactate steady state (or an oxygen uptake steady state) and does not correspond to a power output which can be sustained a long time. The authors present experimental data collected during local (knee extension) and general (running and cycling) exercises which suggest that critical power could correspond to a steady state provided that critical power is calculated from heavy submaximal exercises only (t(lim) ranging between 6 and 30 min). It is difficult to predict exhaustion time from critical power or critical velocity because of the hyperbolic nature of the power-t(lim) relationship. On the other hand, a large error in the measure of t(lim) should have a small effect on the calculation of critical power or velocity. In contrast, the value of Y intercept of the Wlim-t(lim) (or Dlim-(t(lim)) relationship should be sensitive to errors in t(lim).

Sex-linked differences in equilibrium reactions among adolescents performing complex sensorimotor tasks.

Equilibrium reactions were compared between male and female adolescents (prepuberal and puberal), classified into two groups: those who had previously learned complex motor tasks (dance or acrobatics) and those with no particular training. Subjects stood (eyes open or eyes closed) on a free seesaw platform, the displacements of which were calculated from accelerometer measures. They were instructed to maintain a vertical position with their frontal plane either parallel (to measure antero-posterior oscillations) or perpendicular to the axis of the platform (to measure lateral oscillations). Girls had a better stability than boys as shown by the smaller displacement of their center of gravity. Untrained subjects, irrespective of sex, were the least stable. Subjects trained in acrobatics were more stable than dancers. Differences related to sex can be attenuated by physical training involving equilibrium exercises which suggests that moderate sustained training could reduce the incidence of falls in aged persons and in professionally exposed workers.

Effects of aerobic exercise on the torque-velocity relationship in cycling.

The kinetics of the torque-velocity (T-omega) relationship after aerobic exercise was studied to assess the effect of fatigue on the contractile properties of muscle. A group of 13 subjects exercised until fatigued on a cycle ergometer, at an intensity which corresponded to 60% of their maximal aerobic power for 50 min (MAP60%); ten subjects exercised until fatigued at 80% of their maximal aerobic power for 15 min (MAP80%). Of the subjects 7 exercised at both intensities with at least a 1-week interval between sessions. Pedalling rate was set at 60 rpm. The T-omega relationship was determined from the velocity data collected during all-out sprints against a 19 N.m braking torque on the same ergometer, according to a method proposed previously. Maximal theoretical velocity (omega zero) and maximal theoretical torque (Tzero) were estimated by extrapolation of the linear T-omega relationship. Maximal power (Pmax) was calculated from the values of Tzero and omega zero (Pmax = 0.25 omega zero Tzero). The T-omega relationships were determined before, immediately after and 5 and 10 min after the aerobic exercise. The kinetics of omega zero, Tzero and Pmax was assumed to express the effects of fatigue on the muscle contractile properties (maximal shortening velocity, maximal muscle strength and maximal power). Immediately after exercise at MAP60% a 7.8% decrease in Tzero and 8.8% decrease in Pmax was seen while the decrease in omega zero was nonsignificant, which suggested that Pmax decreased in the main because of a loss in maximal muscle strength. In contrast, MAP80% induced a 8.1% decrease in omega zero and 12.8% decrease in Pmax while the decrease in Tzero was nonsignificant, which suggested that the main cause of the decrease in Pmax was probably a slowing of maximal shortening velocity. The short recovery time of the T-omega relationship suggests that the causes of the decrease of torque and velocity are processes which recover rapidly.

Relationship between maximal oxygen uptake on different ergometers, lean arm volume and strength in paraplegic subjects.

The present experiment was designed to study the importance of strength and muscle mass as factors limiting maximal oxygen uptake (VO2max) in wheelchair subjects. Thirteen paraplegic subjects [mean age 29.8 (8.7) years] were studied during continuous incremental exercises until exhaustion on an arm-cranking ergometer (AC), a wheelchair ergometer (WE) and motor-driven treadmill (TM). Lean arm volume (LAV) was estimated using an anthropometric method based upon the measurement of various circumferences of the arm and forearm. Maximal strength (MVF) was measured while pushing on the rim of the wheelchair for three positions of the hand on the rim (-30 degrees, 0 degrees and +30 degrees). The results indicate that paraplegic subjects reached a similar VO2max [1.23 (0.34) 1 x min(-1), 1.25 (0.38) 1 x min(-1), 1.22 (0.18) 1 x min(-1) for AC, TM and WE, respectively] and VO2max/body mass [19.7 (5.2) ml x min(-1) x kg(-1), 19.5 (6.14) ml x min(-1) x kg(-1), 19.18 (4.27) ml x min(-1) x kg(-1) for AC, TM and WE, respectively on the three ergometers. Maximal heart rate f((c) (max)) during the last minute of AC (173 (17) beats x min(-1)], TM [168 (14) beats x min(-1)], and WE [165 (16) beats x min(-1)], were correlated, but f((c) (max)) was significantly higher for AC than for TM (P < 0.03). There were significant correlations between MVF and LAV (P < 0.001) and between the MVF data obtained at different angles of the hand on the rim [311.9 (90.1) N, 313.2 (81.2) N, 257.1 (71) N, at -30 degrees, 0 degrees and +30 degrees, respectively]. There was no correlation between VO2max and LAV or MVF. The relatively low values of f((c) (max)) suggest that VO2max was, at least in part, limited by local aerobic factors instead of central cardiovascular factors. On the other hand, the lack of a significant correlation between VO2max and MVF or muscle mass was not in favour of muscle strength being the main factor limiting VO2max in our subjects.

The effects of maturation on self-induced dynamic body sway frequencies of girls performing acrobatics or classical dance.

We investigated the effects of maturation on the dynamic body sways of healthy girls. Prepubertal and postpubertal girls practising professional physical activities requiring a good ability to maintain equilibrium (acrobats and dancers) were asked to stand on a free seesaw platform and the results compared to those for untrained age-matched girls. This platform (stabilometer) allows self-induced body sways. Stabilograms were obtained by a double integration of the angular acceleration from the recordings of the platform sways made with an accelerometer. Fast Fourier transform processing of stabilograms allowed spectral frequency analysis. The total spectrum energy and the energies of three frequency bands (0-0.5 Hz, 0.5-2 Hz, 2-20 Hz) were determined. ANOVA showed that, for all groups of different equilibrium activity and independent of visual input, prepubertal girls had higher energy values than postpubertal girls in the 0- to 0.5-Hz band whereas the opposite was true for 0.5- to 2-Hz band. Ballet dancers were more dependent than acrobats on visual inputs for the regulation of their postural control but were less dependent than untrained girls at both ages. Maturation seemed to shift body sways towards higher frequencies and the utilization of the cues of postural control was different according to the type of equilibrium activity practised by the subjects.

Influence of posture and training on the endurance time of a low-level isometric contraction.

Classically, the critical force of a muscle (the relative force below which an isometric contraction can be maintained for a very long time without fatigue) is comprised of between 15 and 20% of its maximum voluntary contraction (MVC). However, some authors believe that the value is below 10% MVC. If such is the case, signs that accompany the establishment of muscle fatigue (EMG changes, continuous increase in systolic blood pressure [SBP] and heart rate [HR]) would have to appear more rapidly and with a higher intensity if the muscle is already partially fatigued at the start of maintaining a contraction at 10% MVC. Twelve healthy untrained participants carried out two isometric contractions with the digit flexors: one (test A) began with a maximum contraction sustained for 4 min followed without interruption by a contraction at 10% MVC for 61 min; the other (test B) was a contraction maintained at 10% MVC for 65 min. For test B, after an initial increase of 4 bpm with respect to at rest, HR remained stable until the end of contraction, SBP progressively increased by 24 mm Hg in 28 min, then remained unchanged until the end, and there were no significant changes in EMG (absence of spectral deviation towards low frequencies). For test A, in spite of the initial maximum contraction, changes in the parameters being studied (total maintenance time, HR, SBP, EMG) during maintenance at 10% MVC were identical to those for test B. The results show that (1) when the number and intensity of the co-contractions are minimized by applying an appropriate posture, it is possible to sustain an isometric contraction at 10% MVC for at least 65 min without the appearance of signs of muscle fatigue; (2) the critical force of the digit flexors is higher than 10% MVC.

Physiological effects of downhill skiing at moderate altitude in untrained middle-aged men.

To evaluate whether occasional strong physical activity at moderate altitude for several consecutive days is acceptable in untrained middle-aged people, 10 men (age range, 46-59 years) underwent physical examinations before (control day, D0), during (D1-D8), and after 1 wk of leisure alpine skiing. With respect to D0, the resting concentration of plasma noradrenaline (NOR) increased transiently (p < 0.01) on D2 and then increased to a maximal value from D6-D8 (p < 0.01). There was no significant change in the concentration of adrenaline. Although maximal voluntary contraction of knee extensors diminished on D3 (P < 0.05), that of the digit flexors did not change. Heart rate (HR) and blood pressure at rest in the evening were always higher than control values except on D4 (forced rest). After the stay, there was a reduction in sympathetic activity. This was reflected by a return of NOR to its control value, a decrease in resting HR (64.2 [11.4] beats per minute [bpm]: control: 71.1 [10.1] bpm, P < 0.02), a tendency for triglyceride and insulin resistance to decrease, and a significant increase in alipoprotein A1/alipoprotein A2 (P < 0.01). Our results show that despite signs of fatigue on D3, the effects of physical activity that is relatively intense (HR > 70% maximal HR) together with mild hypoxia are well tolerated by untrained middle-aged men and that the controlled practice of downhill skiing may be accepted into a program to lower cardiovascular risk factors.

Use of physiological criteria for improving physical work conditions.

Physical work is still present in many countries, specially in developing ones. The physiological analysis of the work load could be conducted through two different ways, referring to his energetic cost and/or to the mechanical power of the human motor. The purpose of ergonomics is to organize any physical professional task in order to maintain the workers as much as possible inside the limits of a fatiguing activity. The process of fatigue affects differently a "general activity" and a "local work." If a worker is engaged in a general hard activity, like carrying a load or pushing a trolley, an increase of oxygen consumption or/and an acceleration of the heart rate could be observed. This latter, allowing to estimate the oxygen cost of the work, is more affected if the work is performed in a hot environment. In order to establish the ratio between the data observed at the work place and the maximal working capacity of the organism, it is possible to experiment with the physiological effects of transitory increases of the work stress, by increasing the load or/and the frequency of the repetition of the "unit" of work done. When the task could be described in terms of local work, the correct methodology is based upon the general rules established respectively for the static and the dynamic work. In both the cases, it is possible to determine the critical rate of the muscular activity, the part of the muscular capacity engaged at the work place and the probable time for local exhaustion, in order to limit the duration of each period of work. It should be remembered that 50 years ago, Lucien Brouha proposed that heart rate determinations at work be used as a criteria for a better organization of human work by a non-sophisticated method.