RESUMEN
Boysenberry, a hybrid Rubus berry, is mainly cultivated in New Zealand. We previously reported that consumption of boysenberry juice (BBJ) exhibited anti-obesity effects in high-fat feeding rats. In this study, we focused on the suppressive effect of BBJ and its fraction on triglyceride absorption from the gastrointestinal tract. BBJ effectively inhibited pancreatic lipase activity in vitro, and was separated into four fractions (Fr1, Fr2, Fr3 and Fr4) by HP-20 column chromatography. Among all the fractions, Fr3, the ellagic acid-rich fraction, showed the most potent inhibition against pancreatic lipase in vitro with Fr2, the anthocyanin-rich fraction, second. Authentic ellagic acid equivalent in Fr3 showed poor activity against pancreatic lipase. Then, each fraction was orally administered with corn oil to rats fitted with a jugular catheter to examine the effects of each fraction on plasma triglyceride levels. Both Fr2 and Fr3 effectively suppressed the plasma triglyceride level elevation at a dose of 1,000 mg/kg body weight. These findings demonstrated that BBJ contains chemical components which inhibit triglyceride absorption from the gastrointestinal tract.
Asunto(s)
Jugos de Frutas y Vegetales , Frutas/química , Polifenoles/farmacología , Rubus , Triglicéridos/sangre , Animales , Tracto Gastrointestinal/metabolismo , Lipasa/efectos de los fármacos , Masculino , Páncreas/efectos de los fármacos , Páncreas/enzimología , Ratas , Ratas WistarRESUMEN
Four new chromone alkaloids, chrotacumines G-J (1-4), have been isolated from the barks of Dysoxylum acutangulum. Their structures and absolute configurations were elucidated on the basis of NMR and CD data. Chrotacumines G and J (1 and 4) showed osteoclast differentiation inhibitory activity in a dose dependent manner.
Asunto(s)
Alcaloides/farmacología , Cromonas/farmacología , Meliaceae/química , Osteoclastos/efectos de los fármacos , Piperidinas/farmacología , Alcaloides/química , Alcaloides/aislamiento & purificación , Diferenciación Celular/efectos de los fármacos , Cromonas/química , Cromonas/aislamiento & purificación , Relación Dosis-Respuesta a Droga , Humanos , Estructura Molecular , Osteoclastos/citología , Piperidinas/química , Piperidinas/aislamiento & purificación , Corteza de la Planta/química , Relación Estructura-ActividadRESUMEN
Two new limonoids, sanjecumins A (1) and B (2), have been isolated from the leaves of Sandoricum koetjape, together with sandoripins A (3) and B (4). Their structures and absolute configurations were elucidated on the basis of NMR and CD data. Sandoripins A (3) and B (4) moderately inhibited nitric oxide production in mouse macrophage-like cell line J774.1 stimulated by lipopolysaccharide.
Asunto(s)
Limoninas/química , Limoninas/farmacología , Meliaceae/química , Animales , Línea Celular , Lipopolisacáridos/farmacología , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Espectroscopía de Resonancia Magnética , Ratones , Estructura Molecular , Óxido Nítrico/metabolismo , Hojas de la Planta/químicaRESUMEN
Five new limonoids, ceramicines E-I (1-5), have been isolated from the bark of Chisocheton ceramicus. The structures and relative stereochemistry of them were fully elucidated based on 1D- and 2D-NMR data. Ceramicines E-I (1-5) exhibited moderate cell growth inhibitory activities on a range of cell lines (HL-60, A549, MCF7, and HCT116). The absolute structure of previously isolated ceramicine B (6) was also elucidated by circular dichroism (CD) and X-ray analysis.
Asunto(s)
Limoninas/química , Meliaceae/química , Línea Celular Tumoral , Dicroismo Circular , Cristalografía por Rayos X , Humanos , Limoninas/aislamiento & purificación , Limoninas/toxicidad , Espectroscopía de Resonancia Magnética , Conformación Molecular , Corteza de la Planta/químicaRESUMEN
Four new chromone alkaloids, chrotacumines A-D (1-4), consisting of a 5,7-dihydroxy-2-methylchromone, an N-Me piperidine ring, and an ester side chain were isolated from Dysoxylum acutangulum, and their structures including absolute configurations were elucidated on the basis of spectroscopic data interpretation including 2D NMR, CD spectra, and X-ray analysis. The known compound rohitukine (5) showed moderate cytotoxicity against human HL-60 promyelocytic leukemia and HCT-116 colon cancer cells.