RESUMEN
The formation process of Pt decorated Ni-Pt nanocubes was investigated by analysing the elemental distribution of Ni and Pt in the particles obtained from time-resolved in situ sampling during the synthesis in the oleylamine-1-heptanol system. The analysis confirmed the formation of Pt(core)-Ni(shell) nanoparticles at the initial stages of the reaction. However, as the reaction time progressed, the Pt atoms at the centre diffused outward and reached the corners and edges of the particle, whose shape changed from nearly spherical at the initial stages of the reaction to a perfect cube at the end of the reaction, forming a Ni rich cube (core)-Pt(cage). The cage obtained by dissolving the Ni rich cube was composed mainly of Pt and the Ni content in the frame was a mere 12%. The catalytic activity of the Pt cage was measured using cyclic voltammetry. The initial measurements suggested that the activity was comparable to some of the commercially available Pt catalysts.
Asunto(s)
Anticuerpos/inmunología , Factor VIII/inmunología , Hemofilia A/inmunología , Tolerancia Inmunológica , Terapia Recuperativa , Factor de von Willebrand/inmunología , Niño , Preescolar , Relación Dosis-Respuesta a Droga , Hemofilia A/complicaciones , Hemorragia/etiología , Humanos , Recién Nacido , JapónAsunto(s)
Deficiencia del Factor VII/tratamiento farmacológico , Deficiencia del Factor VII/cirugía , Factor VIIa/administración & dosificación , Adulto , Artroplastia de Reemplazo de Cadera , Deficiencia del Factor VII/complicaciones , Femenino , Hemartrosis/etiología , Hemartrosis/cirugía , Articulación de la Cadera/patología , Humanos , Periodo Intraoperatorio , Japón , Proteínas Recombinantes/administración & dosificación , Resultado del TratamientoAsunto(s)
Angioedema/patología , Enfermedades Intestinales/patología , Intestino Delgado/patología , Adulto , Angioedema/diagnóstico por imagen , Angioedema/etiología , Angioedema/terapia , Femenino , Humanos , Enfermedades Intestinales/diagnóstico por imagen , Enfermedades Intestinales/etiología , Enfermedades Intestinales/terapia , Mucosa Intestinal/patología , Intestino Delgado/diagnóstico por imagen , Masculino , Tomografía Computarizada por Rayos XRESUMEN
The dielectric relaxation spectra of alcohol/nonpolar solute mixtures are measured at several temperatures (-15 degrees C < or = T < or = 25 degrees C) and for several molar fractions of solute (0 < or = X(s) < or = 0.114) in the frequency range of 200 MHz < or = nu < or = 20 GHz. The double-Debye-type function is used for fitting of the spectra of mixtures, and the mean dielectric relaxation times (tau(mean)) of alcohol molecules are determined. In the systems having strong interaction between alcohol and nonpolar solutes, tau(mean) becomes shorter with an increase in the concentration of the solutes. On the other hand, tau(mean) becomes longer in the system having weak interaction between alcohol and nonpolar solutes. These results contradict with our intuitive predictions, do not correspond to mixing enthalpy, and are not explained by the hydrodynamic theory. They are attributed to the mechanism of the coupling between long-range electrostatic interactions and concentration fluctuation caused by the addition of solutes, which is suggested by Yamaguchi et al. based on the mode-coupling theory (Yamaguchi, T.; Matsuoka, T.; Koda, S. J. Chem. Phys. 2004, 120, 7590).
Asunto(s)
Etanol/química , Metanol/química , Solventes/química , Fenómenos Químicos , Química Física , Congelación , Cómputos Matemáticos , TermodinámicaRESUMEN
We evaluated the ability of human anti-lipopolysaccharide (LPS) O6 immunoglobulin G (IgG) and IgM antibodies to protect mice challenged with Escherichia coli serotype O6:K2ac. Purified whole IgG, commercial gammaglobulin, whole IgM-effluent, pool of normal human serum (NHS), agammaglobulinaemic serum (test groups) or phosphate-buffered saline (control group) was injected into adult male 18 h before a challenge with viable O6 E. coli. The mortality rate was assessed over a period of 72 h. To determine the opsonic and phagocytic activity of the antibody isotypes, we incubated peritoneal macrophages from the control and test groups collected at different times after challenge with the live bacteria with acridine orange for fluorescent analysis. Tumour necrosis factor (TNF)-alpha and interleukin (IL)-6 were quantified in serum of both the test and control groups. All mice that received commercial gammaglobulin or NHS survived. Purified whole IgG (containing 1.1 mg/l of anti-LPS O6 IgG antibodies) protected 87.5% of the animals tested in this experiment, while whole IgM-enriched effluent with 1.5 mg/l of anti-LPS O6 IgM antibodies protected only 12.5%. The agamma serum showed no protective capacity compared with PBS (serving as control). The minimal concentration of anti-LPS O6 IgG antibodies able to protect 50% of animals was 0.137 mg/l of purified whole IgG. Whole IgM-enriched effluent showed no protective capacity independently of the concentration tested (0.048-17.0 mg/l of anti-LPS O6 IgM antibodies). Fluorescent analysis of peritoneal macrophages from animals pretreated with purified whole IgG showed no bacteria at 8 h after the challenge. By contrast, whole IgM effluent showed an increasing number of live bacteria at the same time. Mice that had received whole IgM effluent (1.5 mg/l of anti-LPS O6 IgM antibodies) before the challenge with LPS O6 presented 20.5 microg/l of IL-6 and 1.5 microg/l of TNF-alpha. Serum from animals pretreated with purified IgG did not present any detectable pro-inflammatory cytokine. Our findings suggest that IgG but not IgM antibodies protect animals from a challenge with E. coli O6 serotype.
Asunto(s)
Infecciones por Escherichia coli/inmunología , Infecciones por Escherichia coli/prevención & control , Vacunas contra Escherichia coli/administración & dosificación , Escherichia coli/inmunología , Inmunización Pasiva , Inmunoglobulina G/farmacología , Inmunoglobulina M/farmacología , Animales , Formación de Anticuerpos , Antígenos Bacterianos/inmunología , Antígenos Bacterianos/farmacología , Relación Dosis-Respuesta a Droga , Vacunas contra Escherichia coli/inmunología , Vacunas contra Escherichia coli/uso terapéutico , Humanos , Interleucina-6/sangre , Lipopolisacáridos/administración & dosificación , Lipopolisacáridos/inmunología , Lipopolisacáridos/farmacología , Masculino , Ratones , Ratones Endogámicos , Fagocitosis/inmunología , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
Abstract Although many bamboo species are characterized by simultaneous flowering at long intervals of up to 120 years, few studies have revealed the length of the flowering interval for very-long-lived bamboo species by observing the whole life cycle of a single clone. The flowering interval of Phyllostachys pubescens had been determined to be 67 years by means of observation through the entire life cycle from 1912 to 1979. We observed and analysed the clonal structure and flowering traits of a P. pubescens community which had regenerated from seed in 1930 and began to flower in 1997. Although this interval was again 67 years, flowering and nonflowering culms were mixed, and the flowering event lasted three years in the community. AFLP analysis of DNA samples showed distinct genets that originated from the previous flowering event and that each genet had its own flowering time. This is the first report to show that (i) different genets are mixed in a community of a large bamboo species with a leptomorphic rhizome system, and (ii) a community that originated from a single flowering event can have a range of flowering years.
Asunto(s)
Flores/fisiología , Poaceae/genética , Poaceae/fisiología , Simulación por Computador , Genotipo , Japón , Técnicas de Amplificación de Ácido Nucleico , Poaceae/crecimiento & desarrollo , Polimorfismo de Longitud del Fragmento de Restricción , Reproducción/fisiología , Factores de TiempoRESUMEN
An in-hospital 72-year-old male with complaint of anterior chest pain was presented with shock. Pericardial effusion was confirmed by echocardiography, and therefore, he was brought to the operating room immediately with diagnosis of ventricular free wall rupture. The repair was performed using fibrin glue, fibrin sheet, and pericardial patch. His post-operative course was uneventful until massive bleeding came through the chest tube on the 5th day from the initial surgery. The emergency sternotomy was made again for hemostasis. In the re-operation, an approximately 20 mm laceration along the proximal circumflex artery was found and this was successfully repaired with 2 pledgetted horizontal mattress sutures. It is important and necessary to treat a postoperative patient taking care of re-rupture.
Asunto(s)
Rotura Cardíaca Posinfarto/cirugía , Anciano , Ventrículos Cardíacos , Humanos , Masculino , Recurrencia , ReoperaciónRESUMEN
We investigated whether various carotenoids present in foodstuffs were potentially involved in cancer-preventing action on human prostate cancer. The effects of 15 kinds of carotenoids on the viability of three lines of human prostate cancer cells, PC-3, DU 145 and LNCaP, were evaluated. When the prostate cancer cells were cultured in a carotenoid-supplemented medium for 72 h at 20 micromol/L, 5,6-monoepoxy carotenoids, namely, neoxanthin from spinach and fucoxanthin from brown algae, significantly reduced cell viability to 10.9 and 14.9% for PC-3, 15.0 and 5.0% for DU 145, and nearly zero and 9.8% for LNCaP, respectively. Acyclic carotenoids such as phytofluene, zeta-carotene and lycopene, all of which are present in tomato, also significantly reduced cell viability. On the other hand, phytoene, canthaxanthin, beta-cryptoxanthin and zeaxanthin did not affect the growth of the prostate cancer cells. DNA fragmentation of nuclei in neoxanthin- and fucoxanthin-treated cells was detected by in situ TdT-mediated dUTP nick end labeling (TUNEL) assay. Neoxanthin and fucoxanthin were found to reduce cell viability through apoptosis induction in the human prostate cancer cells. These results suggest that ingestion of leafy green vegetables and edible brown algae rich in neoxanthin and fucoxanthin might have the potential to reduce the risk of prostate cancer.
Asunto(s)
Carotenoides/farmacología , División Celular/efectos de los fármacos , Neoplasias de la Próstata/patología , Humanos , Masculino , Células Tumorales CultivadasRESUMEN
Despite the interest in the beneficial roles of dietary carotenoids in human health, little is known about their solubilization from foods to mixed bile micelles during digestion and the intestinal uptake from the micelles. We investigated the absorption of carotenoids solubilized in mixed micelles by differentiated Caco-2 human intestinal cells, which is a useful model for studying the absorption of dietary compounds by intestinal cells. The micelles were composed of 1 micromol/L carotenoids, 2 mmol/L sodium taurocholate, 100 micromol/L monoacylglycerol, 33.3 micromol/L fatty acid and phospholipid (0-200 micromol/L). The phospholipid content of micelles had profound effects on the cellular uptake of carotenoids. Uptake of micellar beta-carotene and lutein was greatly suppressed by phosphatidylcholine (PC) in a dose-dependent manner, whereas lysophosphatidylcholine (lysoPC), the lipolysis product of PC by phospholipase A2 (PLA2), markedly enhanced both beta-carotene and lutein uptake. The addition of PLA2 from porcine pancreas to the medium also enhanced the uptake of carotenoids from micelles containing PC. Caco-2 cells could take up 15 dietary carotenoids, including epoxy carotenoids, such as violaxanthin, neoxanthin and fucoxanthin, from micellar carotenoids, and the uptakes showed a linear correlation with their lipophilicity, defined as the distribution coefficient in 1-octanol/water (log P(ow)). These results suggest that pancreatic PLA2 and lysoPC are important in regulating the absorption of carotenoids in the digestive tract and support a simple diffusion mechanism for carotenoid absorption by the intestinal epithelium.
Asunto(s)
Carotenoides/farmacocinética , Intestinos/efectos de los fármacos , Lisofosfatidilcolinas/farmacología , Ácidos y Sales Biliares/fisiología , Células CACO-2 , Humanos , Mucosa Intestinal/metabolismo , Intestinos/enzimología , Fosfolipasas A/metabolismo , Fosfolipasas A2RESUMEN
BACKGROUND: Fanconi's anemia (FA) is a rare recessive chromosomal instability disorder, characterized by progressive bone marrow failure and congenital defects. Patients with FA present with recurrent infections, particularly those of the respiratory tract. OBJECTIVE: The aim of the present study was to evaluate whether patients with FA have altered antibody-mediated immune responses. METHODS: A group of 12 patients with FA, 5-32 years old (6 males) was studied. Serum levels of IgG, IgM, IgA and IgG subclasses, isohemagglutinin titers and specific IgG antibodies to poliovirus and measles were determined using standard methods. Immediate skin tests to common inhalant allergens were performed, and total and specific serum IgE was quantitated using a fluoroenzymatic assay (Uni-CAP, Pharmacia). Antipneumococcal antibodies were measured by ELISA before and 4-8 weeks after immunization with pneumococcal vaccine (Pneumo 23, Pasteur Mérieux Connaught). Responses to serotypes 1, 3, 5, 6B, 9V and 14, which are the most prevalent in our country, were studied. RESULTS: Ten patients had elevated IgE levels in sera, and 7 of them had detectable specific IgE and positive immediate skin tests. An inadequate response to pneumococcal vaccination was found in 2 of the 12 patients. Isohemagglutinin titers and levels of IgG, IgM, IgA and IgG subclasses and antipoliovirus and antimeasles antibodies were within the normal limits for age in all patients. Two patients had undetectable IgG4 levels (below 5 mg/dl). CONCLUSIONS: The results indicate that a proportion of patients with FA (2/12) in our study had inadequate responses to pneumococcal vaccination. No other significant abnormalities of the immune system were found in these patients.
Asunto(s)
Anemia de Fanconi/inmunología , Adolescente , Adulto , Formación de Anticuerpos , Niño , Preescolar , Femenino , Humanos , Inmunización , Inmunoglobulina E/sangre , Inmunoglobulina G/sangre , Inmunoglobulina G/clasificación , Masculino , Vacunas Neumococicas/inmunologíaRESUMEN
A high prevalence of systemic infections caused by enterobacteria such as Escherichia coli is observed during the neonatal period. Lipopolysaccharide (LPS) is one of the major factors responsible for septic shock caused by these Gram-negative bacteria. We have recently demonstrated the presence of anti-LPS immunoglobulin (Ig)G antibodies in cord blood with a repertoire identical to that found in maternal serum. In the present study, we analyzed anti-LPS O111 antibody isotypes in maternal serum and colostrum from mothers and in cord serum from their respective full-term (n = 30) and preterm (n = 13) neonate infants. The main isotype found in serum samples from mothers of term infants was IgM (range between 28 and 54 mg/l), followed by IgA (1-2 mg/l) and IgG (2-3 mg/l). The range of IgG antibody concentrations in cord blood was between 2 and 3 mg/l, as a result of placental transfer. A novel observation in our study was that the LPS bands recognized by colostral antibodies were completely different from those recognized by IgG in serum. Colostral IgA antibodies recognized several bands not bound by serum IgG antibodies from the respective maternal serum, independently of the antibody quantity. In addition, we verified the pattern of LPS recognition by serum IgA and colostral IgA antibodies was identical, what suggested that the antibody isotype found in serum could probably be derived from differentiated IgA-positive cells which were homing to the mucosa through the mucosal homing mechanism. Identical pattern of recognition was obtained comparing the IgA and IgM isotypes in colostrum. Slight differences in the pattern of recognition were found between colostral and serum IgM antibodies. The fact that colostral antibodies recognize much more bands than serum antibodies may be important for the host to mount an effective immune response in the intestinal lumen, in order to prevent excessive absorption of LPS, reducing possible systemic effects caused by the molecule.
Asunto(s)
Anticuerpos Antibacterianos/biosíntesis , Especificidad de Anticuerpos , Escherichia coli/inmunología , Recién Nacido/inmunología , Lipopolisacáridos/inmunología , Western Blotting , Calostro/inmunología , Femenino , Sangre Fetal/inmunología , Humanos , Inmunoglobulina A/biosíntesis , Inmunoglobulina G/biosíntesis , Inmunoglobulina M/biosíntesis , EmbarazoRESUMEN
In flowering plants, flower organ identity is controlled by the ABC genes, including several MADS box genes. We present two MADS box genes of a conifer, Cryptomeria japonica D. Don. The genes, CjMADS1 and CjMADS2, were related to the angiosperm B function genes which determine the identities of petals and stamens. A phylogenetic analysis showed that these genes form a new clade outside the angiosperm B group, that is, PISTILLATA (PI) and APETALA3 (AP3) lineages. CjMADS1 had a PI-group specific motif and CjMADS2 had AP3-group specific motifs at the C terminal end, respectively. CjMADS1 was expressed in male strobili (or cones) throughout its development, while CjMADS2 was transiently expressed during male strobilus development. The specific expression in the male reproductive organ indicated that the B function is maintained in gymnosperms. Our cladistic analysis suggests that the gene duplication event which generated B function gene lineages predates the divergence of angiosperms and gymnosperms and that the gene duplication which produced the two genes of C. japonica occurred in an ancestral conifer species.
Asunto(s)
Cupressaceae/genética , Genes de Plantas , Proteínas de Dominio MADS/genética , Proteínas de Plantas/genética , Secuencia de Aminoácidos , Expresión Génica , Genoma de Planta , Proteínas de Dominio MADS/clasificación , Magnoliopsida/genética , Datos de Secuencia Molecular , Filogenia , Proteínas de Plantas/clasificación , Análisis de Secuencia de ADN , Homología de Secuencia de AminoácidoRESUMEN
The cleavage products formed by autoxidation of lycopene were evaluated in order to elucidate possible oxidation products of lycopene in biological tissues. Lycopene solubilized at 50 microM in toluene, aqueous Tween 40, or liposomal suspension was oxidized by incubating at 37 degrees C for 72 h. Among a number of oxidation products formed, eight products in the carbonyl compound fraction were identified as 3,7,11 -trimethyl-2,4,6,10-dodecatetraen-1-al, 6,10,14-trimethyl-3,5,7,9,13-pentadecapentaen-2-one, acycloretinal, apo-14'-lycopenal, apo-12'-lycopenal, apo-10'-lycopenal, apo-8'-lycopenal, and apo-6'-lycopenal. These correspond to a series of products formed by cleavage in the respective 11 conjugated double bonds of lycopene. The maximal formation of acycloretinal was 135 nM in toluene, 49 nM in aqueous Tween 40, and 64 nM in liposomal suspension. Acycloretinoic acid was also formed by autoxidation of lycopene, although its formation was lower in the aqueous media than in toluene. The pig liver homogenate had the ability to convert acycloretinal to acycloretinoic acid, comparable to the conversion of all-transretinal to all-trans-retinoic acid. These results suggest that lycopene might be cleaved to a series of apolycopenals and short-chain carbonyl compounds under the oxidative conditions in biological tissues and that acycloretinal is further enzymatically converted to acycloretinoic acid.
Asunto(s)
Carotenoides/química , Carotenoides/metabolismo , Animales , Cromatografía Líquida de Alta Presión , Diterpenos , Liposomas , Hígado/metabolismo , Licopeno , Oxidación-Reducción , Retinoides/química , Retinoides/metabolismo , Espectrofotometría Ultravioleta , PorcinosRESUMEN
Lycopene has been known as a potential food component for cancer prevention, since tomato consumption was shown to be associated with reduced risk of certain cancers. We used HL-60 cells as a model of cancer cells to investigate whether acyclic carotenoids, such as phytoene, phytofluene, and zeta-carotene present in tomatoes, other than lycopene, as well as oxidation mixtures of these carotenoids, are potentially involved in the cancer-preventive action of tomatoes. When HL-60 cells were grown in the carotenoid-supplemented medium for 120 hours, zeta-carotene and phytofluene at 10 microM inhibited cell growth to 3.7% and 22.6% of the growth in control culture, respectively, although they were extremely unstable in the culture medium. The oxidation mixture of each carotenoid, which was prepared by incubation in toluene at 37 degrees C for 24 hours, more strongly inhibited cell growth than each intact carotenoid. The growth inhibition by lycopene was remarkably enhanced by its oxidation before supplementation to the medium. Phytofluene, zeta-carotene, and the oxidation mixture of lycopene induced apoptosis in HL-60 cells during incubation for 24 hours. The addition of alpha-tocopherol to the medium did not eliminate growth inhibition by the oxidation mixture of lycopene. These results suggest that the acyclic carotenoids inhibit cell growth through apoptosis induction and that oxidation products of the carotenoids participate in the growth inhibition.
Asunto(s)
Anticarcinógenos/farmacología , Carotenoides/farmacología , División Celular/efectos de los fármacos , Células HL-60/patología , Apoptosis , Diferenciación Celular/efectos de los fármacos , Medios de Cultivo , Estabilidad de Medicamentos , Humanos , Cinética , Licopeno , Solanum lycopersicum/química , Oxidación-Reducción , Tolueno , alfa-Tocoferol/farmacología , zeta Caroteno/farmacologíaRESUMEN
Beta-carotene-15,15'-dioxygenase is an enzyme responsible for providing vertebrates with vitamin A by catalyzing oxidative cleavage of beta-carotene at its central double bond to two molecules of retinal in intestinal cells. However, little data have been reported regarding regulation of the enzyme activity. We have evaluated the effects of antioxidants and dietary flavonoids on the beta-carotene dioxygenase activity in vitro using a pig intestinal homogenate as the enzyme source. 2,6-Di-tert-butyl-4-methylphenol (BHT), a synthetic antioxidant, strongly inhibited the activity at the level of 10(-6) M (a mixed-type inhibition), whereas butylated hydroxyanisole, nor-dihydroguaiaretic acid, n-propyl gallate, and curcumin were moderately inhibitory. Flavonoids such as luteolin, quercetin, rhamnetin, and phloretin remarkably inhibited the dioxygenase activity noncompetitively, whereas flavanones, isoflavones, catechins, and anthocyanidins were less inhibitory. The structure-activity relationship indicated that catechol structure of ring B and a planar flavone structure were essential for inhibition. The enzyme inhibition was also indicated in the cultured Caco-2 cells by the significantly reduced conversion of beta-carotene to retinol when incubated with BHT and rhamnetin at 2 microM and 5 microM, respectively. The results suggest that some dietary antioxidants derived from food sources modulate conversion of beta-carotene to vitamin A in intestinal cells.
RESUMEN
The severely displaced scaphoid fracture, when combined with a scapholunate dissociation, is a very rare condition that commonly is treated by open surgery. The authors present a case treated by arthroscopic-assisted closed reduction, using percutaneous pinning for the scapholunate dissociation and a Herbert/Whipple screw insertion for the scaphoid fracture. The patient was able to return to work four months after surgery. At twenty-seven months after surgery, both the patient's grip power and range of motion in the wrist were acceptable. The authors recommend this procedure as a minimally invasive operation.
Asunto(s)
Artroscopía , Fijación Interna de Fracturas/métodos , Fracturas Óseas/cirugía , Hueso Escafoides/lesiones , Adulto , Fracturas Óseas/diagnóstico por imagen , Humanos , Masculino , Radiografía , Rango del Movimiento Articular , Hueso Escafoides/diagnóstico por imagenRESUMEN
Beneficial effects of dietary phytic acid (myo-inositol hexaphosphate; IP6) have often been explained by its strong iron ion-chelating ability, which possibly suppresses iron ion-induced oxidative damage in the gastrointestinal tract. Because phytic acid is hydrolyzed during digestion, this work aimed to know whether its hydrolysis products (IP2, IP3, IP4, and IP5) could still prevent iron ion-induced lipid peroxidation. Studies using liposomal membranes demonstrated that hydrolysis products containing three or more phosphate groups are able to inhibit iron ion-induced lipid peroxidation although their effectiveness decreased with dephosphorylation. Similarly, they also prevented iron ion-induced decomposition of phosphatidylcholine hydroperoxide. These results demonstrate that intermediate products of phytic acid hydrolysis still possess iron ion-chelating ability, and thus they can probably prevent iron ion-induced lipid peroxidation in biological systems.
Asunto(s)
Hierro/metabolismo , Peroxidación de Lípido , Liposomas/metabolismo , Membranas/metabolismo , Ácido Fítico/metabolismo , Antioxidantes/farmacología , Quelantes/metabolismo , Cromatografía Líquida de Alta Presión , Hidrólisis/efectos de los fármacos , Fosforilación , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismo , Factores de TiempoAsunto(s)
Trasplante de Corazón/inmunología , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Adulto , Enfermedad de la Arteria Coronaria/sangre , Enfermedad de la Arteria Coronaria/inmunología , Humanos , Periodo Posoperatorio , Valores de Referencia , Estadísticas no Paramétricas , Trasplante HomólogoRESUMEN
To determine the antioxidant activity of dietary quercetin (3,3',4', 5,7-pentahydroxyflavone) in the blood circulation, we measured the inhibitory effect of quercetin metabolites and their related derivatives on copper ion-induced lipid peroxidation of human low-density lipoprotein (LDL). Conjugated quercetin metabolites were prepared from the plasma of rat 1 h after oral administration of quercetin aglycone (40 micromol/rat). The rate of cholesteryl ester hydroperoxide (CE-OOH) accumulation and the rate of alpha-tocopherol consumption in mixtures of LDL solution (0.4 mg/ml) with equal volumes of this preparation were slower than the rates in mixtures of LDL with preparations from control rats. The concentrations of CE-OOH after 2 h oxidation in the mixtures of LDL with preparations of conjugated quercetin metabolites were significantly lower than those in the control preparation. It is therefore confirmed that conjugated quercetin metabolites have an inhibitory effect on copper ion-induced lipid peroxidation in human LDL. Quercetin 7-O-beta-glucopyranoside (Q7G) and rhamnetin (3,3',4', 5-tetrahydroxy-7-methoxyflavone) exerted strong inhibition and their effect continued even after complete consumption, similarly to quercetin aglycone. The effect of quercetin 3-O-beta-glucopyranoside (Q3G) did not continue after its complete consumption, indicating that the antioxidant mechanism of quercetin conjugates lacking a free hydroxyl group at the 3-position is different from that of the other quercetin conjugates. The result that 4'-O-beta-glucopyranoside (Q4'G) and isorhamnetin (3,4',5, 7-tetrahydroxy-3'-methoxyflavone) showed little inhibition implies that introduction of a conjugate group to the position of the dihydroxyl group in the B ring markedly decreases the inhibitory effect. The results of azo radical-induced lipid peroxidation of LDL and the measurement of free radical scavenging capacity using stable free radical, 1,1,-diphenyl-2-picrylhydrazyl, demonstrated that the o-dihydroxyl structure in the B ring is required to exert maximum free radical scavenging activity. It is therefore likely that conjugation occurs at least partly in positions other than the B ring during the process of metabolic conversion so that the inhibitory effect of dietary quercetin is retained in blood plasma after absorption.