In Vivo Studies of a Liquid Embolic Compound.

A composition in the form of liquid polymer substance intended for embolization procedures was studied in in vivo experiment. The preparation was injected to rabbits into the femoral artery and abdominal aorta. The polymer composition exhibited properties previously demonstrated in vitro: strong adhesion to the vascular wall, high plasticity sufficient for embolization of the blood vessels, distal distribution, and the absence of toxic effects. The contrast substance remained in the embolus, which simplified its further localization. The agent underwent nether resorption nor organization. Injection of the agent in a volume of 0.1 ml was sufficient for embolization of an artery with a diameter of 0.1 cm. The polymer composition completely obstructed the vessel without inducing perforation of its wall. During the first day of the experiment, a slight infiltration of surrounding tissues with lymphoid cells was observed. By day 7, total dry necrosis of pelvic limb distal to the injection site was diagnosed. Inflammation of the surrounding tissues was shown histologically and was considered as the body response to impaired circulation and necrosis.

[Comparative evaluation of the immunochromatographic test for detection of hemoglobin.] / Сравнительная апробация Ð¸Ð¼Ð¼ÑƒÐ½Ð¾Ñ Ñ€Ð¾Ð¼Ð°Ñ‚Ð¾Ð³Ñ€Ð°Ñ„Ð¸Ñ‡ÐµÑÐºÐ¾Ð¹ тест-системы для выявления гемоглобина в кале.

The evaluating results of the diagnostic characteristics of an immunochromatographic test for the detection of fecal occult blood (hemoglobin) were presents in the article. The test was approved samples without hemoglobin and model samples containing of standardized preparation of hemoglobin in various concentrations in comparison with two immunochromatographic test systems. The developed test system identified hemoglobin in concentrations higher 50 ng/ml in 99,2% cases, the specificity of the analysis was 98,1%. The obtained levels of sensitivity and specificity were higher the similar parameters for the compared test systems. The immunochromatographic FOB-test can be used for rapid analysis in the primary screening of colorectal cancer.

[Immunochromatographic test for determination of D-dimer.]

The developing and the testing results of an immunochromatographic test for the D-dimer qualitative determination were presents in the article. The test was approved blood plasma samples in comparison with a quantitative enzyme-linked immunosorbent assay (ELISA). The results of assay with developed test were the same with ELISA results for 87,1% and 100% for samples with increased (more than 400 ng/ml FEU) and normal concentration of D-dimer, respectively. The immunochromatographic test for determination of D-dimer can be included in the diagnostic strategy as a cut test after the assessment of venous thromboembolism risk.

[Immunochromatographic test for detection of fecal occult blood.]

The developing and evaluating results of the diagnostic characteristics of an immunochromatographic test for the detection of fecal occult blood (hemoglobin) were presents in the article. The test was approved samples without hemoglobin and model samples containing of standardized preparation of hemoglobin in various concentrations. The developed test system identified hemoglobin in concentrations higher 5 µg / g feces in 97.6% cases, the specificity of the analysis was 100%. The immunochromatographic FOB-test can be used for rapid analysis in the primary screening of colorectal cancer.

Multiplex immunoassay for detection of immunoglobulin G to herpes simplex virus types 1, 2 and cytomegalovirus based on PHOSPHAN technology.

We have developed a multiplex immunoassay test (immunochip) based on PHOSPHAN technology for the detection of immunoglobulin G to herpes simplex virus (HSV) types 1, 2 and cytomegalovirus (CMV). The immunochip consists of HSV type specific gG1 (HSV-1) and gG2 (HSV-2) recombinant antigens, the lysate antigen for detection of total IgG to both HSV types (HSV 1/2), and CMV specific chimeric recombinant antigen containing the immunodominant sequences of pp150, gB, pp28 and pp52 proteins. The sensitivity and specificity of simultaneous IgGs detection with recombinant proteins were comparable to the commercial ELISA kits regardless of the kind of investigated serum specimens (patient sera, standard serum panels). The lysate HSV antigen was as sensitive but significantly less specific, so that it could not be recommended for use as a component of the multiplex test. These results can be used as a basis for creating commercial multiplex tests intended for high-productive screening of HSV, CMV and other TORCH-infections in a clinical laboratory.

[The detection of immunoglobulins G to agents of TORCH-infections using technique of multiplex immunoassay on the basis of FOSFANtm technology.]

The immune chip is developed for detecting immunoglobulins G to agents of four infections of TORCH-complex (toxoplasmosis, German measles, cytomegalovirus and herpes viral infections) on the basis of FOSFANtm technology. The sensitivity and specificity of simultaneous detection of IgG on immune chip were comparable with indices of commercial immunoenzyme test-systems, including under analysis of standard panels of serums. This permits considering derived results as a basis for development of commercial multiplex test intended for highly productive screening of TORCH-infections in clinical laboratory.

[THE SELECTION OF ANTIGENS FOR DETECTING OF IMMUNOGLOBULIN G TO CYTOMEGALOVIRUS ON THE BASIS OF FOSFAN TECHNOLOGY].

The results of selection of composition of antigens to cytomegalovirus in the structure of multiplex test on the basis of FOSFAN™ technique are presented. In the process of detection of immunoglobulin G (IgG) to this virus the best indicators of sensitivity were registered with mosaic antigen containing immunodominant sequences of proteins pp150, gB, pp28 and pp52; reliably lower indicators of sensitivity was registered with phosphoprotein pp150; the lowest indicators of sensitivity were registered with proteins gB and pp65. The specificity made up from 93.5% to 96.8% independently of type of antigen. The mosaic antigen ensured the best ratio between sensitivity and specificity of immunoassay and is considered as the main component of immunochip for detecting IgG to cytomegalovirus.

[Impulsive-compulsive syndrome in Parkinson's disease].

Dopaminergic replacement therapy (DRT) is effective in treatment the motor symptoms of Parkinson's disease (PD) but can lead to impulse control disorders (ICD) in some patients. ICD include pathological gambling, hypersexuality, compulsive shopping, binge eating, punding, dopamine dysregulation syndrome (DDS). Authors studied the prevalence of ICD and its impact on the quality of life and daily activities of PD patients and their relatives. Among 246 patients studied, 55 patients (23%) (28 men, mean age 66.5±9.4 years) were diagnosed with ICD. DDS was noted in 36.4%, punding in 36.4%, binge eating in 23.6%, hypersexuality in 14.5%, compulsive shopping in 14.5% and pathological gambling in 1.8%. Of these 55 patients, 10 (18.1%) had symptoms of 2 of the ICDs: 3 (5.45%) had 3 of the ICDs and 2 (3.63%) patients had 5 of the ICDs. Quality of life ranged from 25% to 89%. Treatment approaches including the adjustment of doses of levodopa and dopamine receptor agonists in PD patients with ICD are presented.

[Endogenous abscisic acid level in wheat plants upon inoculation with the powdery mildew causative agent (Erysiphe graminis f.sp. tritici)]. / Endogennaia abstsizovaia kislota v rasteniiakh pshenitsy pri zarazhenii vozbuditelem muchnistoi rosy (Erysiphe graminis f.sp. tritici).

We studied the dynamics of the abscisic acid content in above-ground parts of wheat cultivars with different resistance against powdery mildew. It was assayed by the HPLC method in healthy and diseased (inoculated by the powdery mildew causative agent) plants (from inoculation to the stage of pathogen spore formation). The results are discussed with special reference to the wheat resistance against the powdery mildew.

[Clinical trial of ventax (long-acting theophylline) in chronic obstructive bronchitis]. / Opyt klinicheskogo primeneniia preparata ventaks (prolongirovannyi teofillin) pri lechenii bol'nykh khronicheskim obstruktivnym bronkhitom.

Clinical evidence obtained on efficacy of a new long-acting theophylline ventax in chronic obstructive bronchitis demonstrates that it has advantages over theophylline, produces positive changes in the patients' external respiration, dyspnea severity, auscultatory parameters, subjective response, is well tolerated and highly effective against chronic obstructive bronchitis. Side effects of ventax are outlined.