Partial mycoheterotrophy in the leafless orchid Eulophia zollingeri specialized on wood-decaying fungi.

Although the absence of normal leaves is often considered a sign of full heterotrophy, some plants remain at least partially autotrophic despite their leafless habit. Leafless orchids with green stems and capsules probably represent a late evolutionary stage toward full mycoheterotrophy and serve as valuable models for understanding the pathways leading to this nutritional strategy. In this study, based on molecular barcoding and isotopic analysis, we explored the physiological ecology of the leafless orchid Eulophia zollingeri, which displays green coloration, particularly during its fruiting phase. Although previous studies had shown that E. zollingeri, in its adult stage, is associated with Psathyrellaceae fungi and exhibits high 13C isotope signatures similar to fully mycoheterotrophic orchids, it remained uncertain whether this symbiotic relationship is consistent throughout the orchid's entire life cycle and whether the orchid relies exclusively on mycoheterotrophy for its nutrition during the fruiting season. Our study has demonstrated that E. zollingeri maintains a specialized symbiotic relationship with Psathyrellaceae fungi throughout all life stages. However, isotopic analysis and chlorophyll data have shown that the orchid also engages in photosynthesis to meet its carbon needs, particularly during the fruiting stage. This research constitutes the first discovery of partial mycoheterotrophy in leafless orchids associated with saprotrophic non-rhizoctonia fungi.

Effects of paddy irrigation-drainage system on water quality and productivity of small rivers in the Himi region of Toyama, Central Japan.

The aim of this study was to clarify the impact of differences between historical and recently introduced irrigation and drainage management systems on water quality in the rivers around paddy fields. We investigated the seasonal variation in nutrients concentration and dissolved organic carbon (DOC) components in single- (used for intake only) and dual-purpose (used for both intake and drainage) channels during a 4-year period in the Himi region of Toyama, Central Japan. The system of dual-purpose channel has traditionally been used in the region of this study. A total of 197 three-dimensional excitation-emission matrix (3DEEM) fluorescence spectra of DOM in waters were applied for the parallel factor analysis (PARAFAC) modeling. Based on the 3DEEM and PARAFAC, the abundance of terrestrial humic-like components in the dual-purpose channel was significantly higher than that in the single-purpose channel. The even long-chain n-fatty acids derived associated with rice cropping in sediments of the dual-purpose channels were 22-30-fold higher than that of the single-purpose channel. In addition, the turbidity values of the river waters had significantly positive linear correlations with concentrations of K+, DOC, and humic-like components. These observations indicate that the dissolved nutrient concentrations in the river water were higher in the dual-purpose channel compared to those of the single-purpose channel, which may be supplied by leaching from the inflow of soil particles from the paddy fields. During the mid-irrigation period, the quantity of epiphytic chlorophyll a on artificial substrate tiles in the dual-purpose channel were 3.1-4.1-fold higher than that in the single-purpose channel. This study clear that the input of paddy drainage during the irrigation season significantly changes the DOC components in river waters and irrigation management is strongly linked to the primary production in agricultural channels. Therefore, it is important to consider the impact of the introduction of different irrigation and drainage management systems on water quality and productivity in order to maintain the riverine ecosystems around rice paddies, which are based on historical water use systems.

Mineral acquisition of Japanese macaques: Contents in the foods, digestibility, and sodium-provisioning experiment.

Minerals provide micronutrients that function in various ways in the body, and they are necessary for the survival of animals. In this study, we first compared the mineral content of foods of wild Japanese macaques in lowland Yakushima with that of monkey chow used for many years to feed captive macaques and specifically formulated to obtain good health in captive macaques (National Research Council [NRC] recommendations). Second, we clarified the mineral balance in captive individuals when feeding them monkey chow to clarify the digestibility/bioavailability of the minerals. Third, we investigated the physiological response when we experimentally increased sodium intake. In the lowland of Yakushima, which is in the vicinity (<800 m) of the coast, animals, fungi, and mature leaves had high sodium contents compared with NRC recommendations. The calcium contents of mature leaves and animals were higher than the NRC recommendations. The overall mineral intake in this population was lower than that in the captive animals for calcium, phosphorus, sodium, and iron, while similar for magnesium and higher in potassium. Patterns in the intake and excretion of minerals indicated that excretion was mostly from urine and not from feces, and apparent digestibility was high for sodium. This tendency was opposite for calcium, magnesium, and phosphorus and intermediate for potassium. A sodium-provisioning experiment showed that fecal aldosterone concentration remained low in both control and sodium-provisioning conditions so the macaques do not need reabsorb sodium in the kidneys. Therefore, sodium content in the monkey chow, which is slightly lower than the NRC recommendation, seemed high enough so that the macaques could avoid the need to reabsorb sodium in the kidneys. We advocate similar studies for other primate populations or species to better understand the role of mineral concentrations on food selection and to identify potential mineral deficiencies.

Water Adsorption to Leaves of Tall Cryptomeria japonica Tree Analyzed by Infrared Spectroscopy under Relative Humidity Control.

Leaf water storage is a complex interaction between live tissue properties (anatomy and physiology) and physicochemical properties of biomolecules and water. How leaves adsorb water molecules based on interactions between biomolecules and water, including hydrogen bonding, challenges our understanding of hydraulic acclimation in tall trees where leaves are exposed to more water stress. Here, we used infrared (IR) microspectroscopy with changing relative humidity (RH) on leaves of tall Cryptomeria japonica trees. OH band areas correlating with water content were larger for treetop (52 m) than for lower-crown (19 m) leaves, regardless of relative humidity (RH). This high water adsorption in treetop leaves was not explained by polysaccharides such as Ca-bridged pectin, but could be attributed to the greater cross-sectional area of the transfusion tissue. In both treetop and lower-crown leaves, the band areas of long (free water: around 3550 cm-1) and short (bound water: around 3200 cm-1) hydrogen bonding OH components showed similar increases with increasing RH, while the band area of free water was larger at the treetop leaves regardless of RH. Free water molecules with longer H bonds were considered to be adsorbed loosely to hydrophobic CH surfaces of polysaccharides in the leaf-cross sections.

Partial mycoheterotrophy in the leafless orchid Cymbidium macrorhizon.

PREMISE OF THE STUDY: The evolution of full mycoheterotrophy is one of the most interesting topics within plant evolution. The leafless orchid Cymbidium macrorhizon is often assumed to be fully mycoheterotrophic even though it has a green stem and fruit capsule. Here, we assessed the trophic status of this species by analyzing the chlorophyll content and the natural 13 C and 15 N abundance in the sprouting and the fruiting season. METHODS: The chlorophyll content was measured in five sprouting and five fruiting individuals of C. macrorhizon that were co-occurring. In addition, their 13 C and 15 N isotopic signatures were compared with those of neighboring autotrophic and partially mycoheterotrophic reference plants. KEY RESULTS: Fruiting individuals of C. macrorhizon were found to contain a remarkable amount of chlorophyll compared to their sprouting counterparts. In addition, the natural abundance of 13 C in the tissues of the fruiting plants was slightly depleted relative to the sprouting ones. Linear two-source mixing model analysis revealed that fruiting C. macrorhizon plants obtained approximately 73.7 ± 2.0% of their total carbon from their mycorrhizal fungi when the sprouting individuals were used as the 100% carbon gain standard. CONCLUSIONS: Our results indicated that despite its leafless status, fruiting plants of C. macrorhizon were capable of fixing significant quantities of carbon. Considering the autotrophic carbon gain increases during the fruiting season, its photosynthetic ability may contribute to fruit and seed production. These results indicate that C. macrorhizon should, therefore, be considered a partially mycoheterotrophic species rather than fully mycoheterotrophic, at least during the fruiting stage.

A test of the effects of timing of a pulsed resource subsidy on stream ecosystems.

Spatial resource subsidies can alter bottom-up and top-down forces of community regulation across ecosystem boundaries. Most subsidies are temporally variable, and recent theory has suggested that consumer-resource dynamics can be stabilized if the peak timing of a subsidy is desynchronized with that of prey productivity in the recipient ecosystem. However, magnitude of consumer responses per se could depend on the subsidy timing, which may be a critical component for community dynamics and ecosystem processes. The aim of this study was to test (i) whether a recipient consumer (cutthroat trout) responds differently to a resource subsidy occurring early in its growing season than to a subsidy occurring late in the season and, if this is the case, (ii) whether the timing-dependent consumer response has cascading effects on communities and ecosystem functions in streams. To test those hypotheses, we conducted a large-scale field experiment, in which we directly manipulated the timing of augmentation of the terrestrial invertebrates that enter stream (i.e. peak timing of June-August vs. August-October), keeping constant the total amounts of the invertebrates entered. We found large increases in the individual growth rate and population biomass of the cutthroat trout, in response to the early resource pulse, but not to the late pulse. This timing-dependent consumer response cascaded down to reduce benthic invertebrates and leaf breakdown rate, and increased water nutrient concentrations. Furthermore, the early resource pulse resulted in higher maturity rate of the cutthroat trout in the following spring, demonstrating the importance of the subsidy timing on long-term community dynamics via the consumer's numerical response. Our results emphasize the need to acknowledge timing-dependent consumer responses in understanding the effects of subsidies on communities and ecosystem processes. Elucidating the mechanisms by which consumers effectively exploit pulsed subsidies is an important avenue to better understand community dynamics in spatially coupled ecosystems.