RESUMEN
Among the primary threats to human health worldwide, nonsmall cell lung cancer (NSCLC) remains a significant factor and is a leading cause of cancer-related deaths. Due to subtle early symptoms, NSCLC patients are diagnosed at advanced stages, resulting in low survival rates. Herein, novel Au-Se bond nanoprobes (NPs) designed for the specific detection of Calpain-2 (CAPN2) and Human Neutrophil Elastase (HNE), pivotal biomarkers in NSCLC, were developed. The NPs demonstrated exceptional specificity and sensitivity toward CAPN2 and HNE, enabling dual-color fluorescence imaging to distinguish between NSCLC cells and normal lung cells effectively. The NPs' performance was consistent across a wide pH range (6.2 to 8.0), and it exhibited remarkable resistance to biological thiol interference, indicating its robustness in complex physiological environments. These findings suggest the nanoprobe is a promising tool for early NSCLC diagnosis, offering a novel approach for enhancing the accuracy of cancer detection.
Asunto(s)
Carcinoma de Pulmón de Células no Pequeñas , Colorantes Fluorescentes , Neoplasias Pulmonares , Humanos , Carcinoma de Pulmón de Células no Pequeñas/diagnóstico , Carcinoma de Pulmón de Células no Pequeñas/patología , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/patología , Colorantes Fluorescentes/química , Imagen Óptica , Oro/química , Calpaína/metabolismo , Calpaína/análisis , Biomarcadores de Tumor/análisis , Biomarcadores de Tumor/metabolismo , Línea Celular TumoralRESUMEN
Novel Au-Se bond-based nanoprobes were designed for concurrent detection of PSA and PSMA in serum samples, aiming to enhance the early diagnosis of prostate cancer. These probes demonstrate robust stability, specificity and accuracy, underscoring their potential as non-invasive tools for diagnosis.
Asunto(s)
Antígenos de Superficie , Colorantes Fluorescentes , Glutamato Carboxipeptidasa II , Oro , Antígeno Prostático Específico , Neoplasias de la Próstata , Humanos , Neoplasias de la Próstata/sangre , Neoplasias de la Próstata/diagnóstico , Masculino , Antígeno Prostático Específico/sangre , Glutamato Carboxipeptidasa II/sangre , Colorantes Fluorescentes/química , Antígenos de Superficie/sangre , Oro/químicaRESUMEN
A Au-Se bond-based nanoprobe using 3',3-diselenopropionic acid to simultaneously link response chains for Pro-GRP protein and Cyfra21-1 was developed. Early diagnosis and subtyping of lung cancer can be achieved based on the nanoprobes' differential response of the probes to the two targets in lung cancer patients' serum.
Asunto(s)
Neoplasias Pulmonares , Humanos , Neoplasias Pulmonares/diagnóstico , Antígenos de Neoplasias , Queratina-19 , Colorantes FluorescentesRESUMEN
A pH-responsive cascade nanoplatform based on ZIF-8 disintegrates in the weakly acidic tumor microenvironment to release MnO2, CaO2 and Ce6. The drugs can cyclically generate O2 for sonodynamic therapy, consume glutathione to tune the redox hemostasis, and produce cytotoxic ËOH to kill tumor cells via chemical dynamics therapy.