RESUMEN
PURPOSE: Poultry by-products can contribute as an innovative natural source for the development of composites based on polymers and minerals aiming at bone regeneration. The objective of this study was the physicochemical and biological characterization of collagen-based hydrogels crosslinked with ultraviolet (UV)-riboflavin. METHODS: Pure hydrogels of 100% collagen (G1) or hybrid hydrogels, 90% collagen:10% apatite (G2), 90% collagen:10% nanokeratin (G3), and 90% collagen:5% apatite:5% nanokeratin (G4) were characterized by scanning electron microscope, Fourier-transform infrared spectroscopy, differential scanning calorimetry, swelling degree and quali-quantitative histological analysis. Ectopic implantation in subcutaneous tissue in mice at one, three and nine weeks allowed to assess the inflammation (neutrophils, lymphocytes, macrophages, and giant cells) and repair (neovascularization, and connective tissue) to determine biocompatibility and the integrity of biomaterials to score their biodegradability. Histomorphometry on critical size defects in rat calvaria at one and three months evaluated the percentage of bone, connective tissue, and biomaterials in all groups. RESULTS: The hydrogels presented porous microstructure, water absorption and physicochemical characteristics compatible with their polymeric and/or mineral composition. All materials exhibited biocompatibility, biodegradability, and low osteoconductivity. G2 showed greater density of new bone and biomaterial than the G1, G3 and G4. CONCLUSIONS: The collagen-apatite group formulation suggests potential for development as osteopromoting membrane.
Asunto(s)
Hidrogeles , Andamios del Tejido , Animales , Apatitas , Materiales Biocompatibles/química , Materiales Biocompatibles/farmacología , Regeneración Ósea , Colágeno/farmacología , Hidrogeles/farmacología , Ratones , Aves de Corral , Ratas , Cráneo/cirugía , Espectroscopía Infrarroja por Transformada de Fourier , Ingeniería de Tejidos/métodos , Andamios del Tejido/químicaRESUMEN
La Odontología está entre las ocupaciones más afectadas por la insatisfacción y el Síndrome de Burnout, un estado de agotamiento mental que incide negativamente en la calidad de vida del trabajador y la eficiencia de los servicios que presta en los Sistemas de Salud Pública. El objetivo de este estudio fue analizar la satisfacción en el trabajo y estimar la prevalencia del Síndrome de Burnout en los profesionales que integran los equipos de salud bucal de atención primaria en el municipio Sobral, Ceará-Brasil. Fue realizado un estudio transversal con 35 Cirujanos-Dentistas (CD) y 15 Técnicos/Auxiliares de Salud Bucal (TSB/ASB), por medio de los cuestionarios Occupational Stress Indicatory Maslach Burnout Inventory-Human Services Survey. Los resultados indicaron distintos niveles de insatisfacción entre los CD y los TSB/ASB; con una prevalencia general baja de Burnout (2%), sin diferencias entre grupos. La alta insatisfacción laboral y mayor expresión parcial de subescalas para Burnout reflejan la importancia de la investigación de estados poco saludables en equipos de Salud Bucal(AU)
Dentistry is one of the occupations most affected by dissatisfaction and burnout syndrome, an mental exhaustion state which negatively affects the worker's quality of life and efficiency of services provided by the national health service. The objective of this study was to analyze job satisfaction and estimate the prevalence of burnout syndrome in oral health professionals who work in primary care centers in the municipality of Sobral, Ceará-Brazil. We performed a cross-sectional study of 35 dentists (D) and 15 dental health technicians/assistants (DAusing the Occupational Stress Indicator and Maslach Burnout Inventory- Human Services Survey questionnaires. The level of dissatisfaction differed between D and DA, with a low overall prevalence of burnout syndrome (2%) and no between-group differences. The high level of job dissatisfaction and greater partial expression of burnout subscales underscore the importance of further researching unhealthy workplace factors in oral health teams(AU)