Research progress in rodent models of endometriosis.

Endometriosis is a common and frequent disease in gynecology; its etiology and pathogenesis are partially understood and still not clear. The construction of suitable animal models is beneficial for basic research related to the disease. Currently, rodents have the advantages of low cost, fast reproduction, easy rearing, and a similar endometrial structure to humans. Depending on the purpose of the experiment, different molding methods have their advantages. In this paper, we describe the traditional methods of constructing endometriosis rodent models, compare their advantages and disadvantages, and introduce newly developed rodent models, such as cell line injection models, pain models, genetically engineered mouse models, fluorescent tracer models, iron overload models, chemical induction models, and methods of constructing rodent models of different subtypes of endometriosis. Fertility and treatment of endometriosis rodent models are also described. This study provides a reference for researchers in the selection of animal models for pathogenesis and drug treatment studies.

The roles of chromatin regulatory factors in endometriosis.

PURPOSE: Endometriosis is an estrogen-dependent inflammatory disease and one of the most common gynecological diseases in women of reproductive age. The aim of the review was to explore the relationship between the chromatin regulatory factors and endometriosis. METHODS: By searching for literature on chromatin regulators and endometriosis in PuMed. Finally, 98 documents were selected. RESULTS: Chromatin regulators (CRs) are essential epigenetic regulatory factors that can regulate chromatin structure changes and are usually divided into three categories: DNA methylation compounds, histone modification compounds, and chromatin remodeling complexes. Noncoding RNAs are also chromatin regulators and can form heterochromatin by binding to protein complexes. Chromatin regulators cause abnormal gene expression by regulating chromatin structure, thereby affecting the occurrence and development of endometriosis. CONCLUSION: This review summarizes the participation of chromatin regulators in the mechanisms of endometriosis, and these changes in related chromatin regulators provide a comprehensive reference for diagnosis and treatment of endometriosis.

Low-CD weakly-coupled FMF for short-reach IM/DD MDM transmission.

Weakly-coupled mode division multiplexing (MDM) technique is a promising candidate for capacity enhancement of short-reach optical interconnections, for which the multiple-ring-core few-mode fiber (MRC-FMF) has been proven to be an effective design method to suppress distributed modal crosstalk. Similar to low chromatic-dispersion (CD) O-band transmission based on single-mode fibers (SMF), all the mode channels in a weakly-coupled FMF for short-reach applications should achieve low CD to support intensity-modulation/direct-detection (IM/DD) transmission. In this paper, we propose, for the first time to the best of our knowledge, an index perturbation method to adjust both effective index and CD of each mode in an MRC-FMF. Firstly, an accurate modeling method to model the relationship between SiO2-GeO2 material index and the germanium concentration at different wavelengths is proposed by analyzing the index characteristics of 4 kinds of germanium-doped fused silica SMFs at the same fabrication processing, which could be utilized to calculate the CD characteristics for an MRC-FMF with perturbed index profile. Then, based on the perturbation method considering the influences on both effective index and CD, a weakly-coupled low-CD MRC-FMF supporting 4 linearly-polarized (LP) modes is designed and fabricated. The measured minimum effective index difference min|Δneff| among all modes is larger than 1.3 × 10-3, and the CD values of all the modes lie between -6 and +6 ps/km/nm ranging from 1280 to 1320 nm, which agree well with the design. The 2-km transmission experiment indicates that the fabricated MRC-FMF could support stable digital-signal-processing (DSP)-free IM/DD transmission for all the 4 LP modes. This work is beneficial to the application of short-reach weakly-coupled MDM systems.

Associations between microbial presence in follicular fluid with IVF outcomes: a systematic review and meta-analysis.

PURPOSE: The aim of the study was to synthesize disparate studies to investigate potential impact of microbial presence in FF of infertile women on IVF outcomes. METHODS: Following preliminary searches to find medical subject heading (MeSH) terms plus free terms, a systematic search was performed in the PubMed, Cochrane Library, Embase, Web of Science, and Clinicaltrials.gov databases from January 10, 2022, to July 5, 2023. Data collected for each study were analyzed using RevMan 5.4 software available on the Cochrane website. RESULTS: After correcting for contamination from the vagina, the FFs of 289 women were detected positively by microbial culture and identification, ELISA, and IPA. The pregnancy rate of the FF-positive group was significantly lower than the FF-negative group (19.7% vs. 32.2%) and (OR: 0.57, 95% CI: 0.28-1.14, P=0.11; I2=56%) while the fertilization rate was almost equal (60.0% vs. 62.0%) and (OR: 1.03, 95% CI: 0.88-1.20, P=0.72; I2=0%). Evidence quality was very low. CONCLUSIONS: The different species of microorganisms in FF of infertile women may have different effects on IVF outcomes. The Lactobacillus spp. may have a positive effect, while other microorganisms may have the opposite effect.

Long non-coding RNA TLR8-AS1 induces preeclampsia through increasing TLR8/STAT1 axis.

OBJECTIVE: Our current study tried to assay the role of long noncoding RNAs (lncRNAs) TLR8-AS1 in regulating preeclampsia. METHODS: TLR8-AS1 expression was examined in the clinical placental tissues of preeclampsia patients and the trophoblast cells induced by lipopolysaccharide (LPS). Then, different lentivirus was infected into trophoblast cells to study the role of TLR8-AS1 in cell functions. Furthermore, interactions among TLR8-AS1, signal transducer and activator of transcription 1 (STAT1) and toll-like receptor 8 (TLR8) were determined. A rat model of preeclampsia induced by N(omega)-nitro-L-arginine methyl ester was developed to validate the in-vitro findings. RESULTS: High expression of TLR8-AS1 was detected in placental tissues of preeclampsia patients and LPS-induced trophoblast cells. In addition, overexpression of TLR8-AS1 arrested the proliferation, migration and invasion of trophoblast cells, which was related to the upregulation of TLR8 expression. Mechanistically, TLR8-AS1 recruited STAT1 to bind to the TLR8 promoter region, and thus promoted the transcription of TLR8. Meanwhile, overexpression of TLR8-AS1 was shown to aggravate preeclampsia by elevating TLR8 in vivo . CONCLUSION: Our study confirmed that TLR8-AS1 aggravated the progression of preeclampsia through increasing the expression of STAT1 and TLR8.

Reducing multipath interference in quasi-single-mode transmission via distributed Raman amplification.

In this Letter, we show numerically and experimentally that multipath interference can be reduced significantly by distributed Raman amplification (DRA) in quasi-single-mode transmission using few-mode fibers, owing to the extra differential mode attenuation induced between the fundamental mode and higher-order modes. The experimental results show that the required number of equalization taps can be reduced by 1.6 times. Moreover, by carefully optimizing the intensity overlap between the LP01 and LP11 modes through structural adjustment of the two-mode fiber, a 3.5-times reduction in the required number of taps can be obtained.

Magnetic sulfur-doped porous carbon for preconcentration of trace mercury in environmental water prior to ICP-MS detection.

A novel magnetic sulfur-doped porous carbon (MSPC) was fabricated via a simple one-step carbonization of a mixture of sucrose, basic magnesium sulfate whiskers and Fe3O4@SiO2 nanoparticles. Due to the high S content, the prepared MSPC possessed high adsorption capacity for Hg2+ (343 mg g-1) with good selectivity. Based on this, a method coupling magnetic solid phase extraction (MSPE) with inductively coupled plasma mass spectrometry (ICP-MS) was developed for the determination of trace Hg2+ in environmental water samples. Various parameters such as pH, desorption solvent and its concentration, desorption volume and time, sample volume, and adsorption time that affect the determination have been optimized. Under the optimal conditions, a high enrichment factor of 100-fold was obtained, the limit of detection (LOD) was found to be 0.52 pg mL-1 with a relative standard deviation (c = 10 pg mL-1, n = 7) of 7.1%, and a good linearity was obtained within the concentration range of 2-5000 pg mL-1 for Hg2+. Besides, the proposed method has very fast adsorption/desorption kinetics, target Hg2+ could be rapidly adsorbed on the prepared MSPC in 2 min and desorbed from the MSPC in 2 min with the assistance of a permanent magnet. Therefore, the proposed method of MSPE-ICP-MS exhibits good application potential in the determination of trace Hg2+ in environmental water samples.