1.
Methods Mol Biol
; 2529: 109-120, 2022.
Artículo
en Inglés
| MEDLINE
| ID: mdl-35733012
RESUMEN
The catalytic activity of histone methyltransferases is not restricted to histones but also includes noncanonical substrates. Increasing evidence shows that histone methyltransferases methylate themselves, and automethylation has emerged as a self-regulatory mechanism. Here, we introduce experimental procedures to identify automethylation sites of histone methyltransferases and to investigate the function of automethylation in a reconstituted biochemical system and in cellular contexts.