[Characteristics of a clinical course of immune cytopenias with a high titer of autoantibodies to the microsomal antigen of the thyroid gland]. / Osobennosti klinicheskogo techeniia immunnykh tsitopenii, soprovozhdaiushchikhsia povyshennym titrom autoantitel k mikrosomal'nomu antigenu shchitovidnoi zhelezy.

AIM: To ascertain the role of high antibodies (Ab) titers to microsomal antigen (MA) of the thyroid in clinical manifestations of cytopenic syndromes. MATERIAL AND METHODS: Clinical data are presented on 144 patients with depressed hemopoiesis having different levels of thyroid Aab. RESULTS: When the titer was significantly elevated (41% patients with immune cytopenias), hematological malignancies run with recurrences, remission is rare. Treatment of the thyroid pathology improves hematological indices in some cases though does not reduce titers of Ab thyroid MA. CONCLUSION: Detection of autoimmune pathology of the thyroid is necessary not only for specification of the concurrent process diagnosis but also for initiation of adequate combined treatment.

[Autoantibodies to the thyroid gland in hemoblastoses and cytopenias]. / Autoantitela k shchitovidnoi zheleze pri gemoblastozakh i tsitopeniiakh.

AIM: To study the level of thyroid antibodies (TAB) in blood diseases. MATERIAL AND METHODS: TAB levels dynamics was studied in 413 patients with hematological diseases. RESULTS: Increased incidence of cases with high Ab titer to thyroid microsomal antigen was found. High and moderate titers were revealed in 33% of all examinees, including 41%, 22%, 32% and 29% among patients with immune cytopenia, chronic lymphoid leukemia, generalized mature-cell lymphoma and myeloproliferative diseases, respectively. These high and moderate titers were rather stable. In 15% patients Ab were elevated insignificantly, this rise being unstable. CONCLUSION: Stable high titers of antibodies to thyroid antigens point to increased incidence rates of concomitant autoimmune hyroiditis in patients with blood diseases.

[Immunogenic peptide neogen stimulates postradiation recovery thrombopoiesis]. / Immunomoduliator neogen stimuliruet postradiatsionnoe vosstanovlenie trombotsitopoéza.

In experiments on mice F1 total exposed to gamma-irradiation at the 5.0 Gy, showed that a intraperitoneal injection neogen at the dose 10 micrograms/kg oue time at the day and night during the 8 days after irradiation results in recovery of the number blood platelets and time issue of blood from standard wound to normal level on the week by compare with irradiated control animals.

[Postradiation recovery of hematopoiesis in mice affected by Neogen (IEW)]. / Postradiatsionnoe vosstanovlenie krovetvoreniia u myshei pod vliianiem neogena (IEW).

The effect of the synthetic peptide IEW (Neogen) with immunomodulating properties on postradiation recovery of haemopoiesis was investigated. We have shown that Neogen is a potential stimulator of haemopoiesis. The administration of Neogen after irradiation shortened duration of period of the recovery of the compartment of CFU-S-8 and the amount of bone marrow cells. The comparision of the effects of Neogen and GM-CSF (Leucomax) and G-CSF (Granocyte 34) have shown that the targets for these agents are probably different: polypotent CFU-S-for Neogen, and CFU-GM-for GM-CFS. Based on the results, we suggested the mechanism of Neogen effects on heamopoiesis.

Synthetic peptide thymodepressin promoted take of transplanted hemopoietic precursor cells in the bone marrow of irradiated mice.

We studied the possibility of using a new synthetic heme-inhibiting peptide thymodepressin for improving the efficiency of transplantations of syngeneic and allogeneic bone marrow. Thymodepressin was injected to recipients 3 times (48, 24, and 2 h) before irradiation and transplantation of bone marrow suspension. The yield of 9- and 12-day colonies increased, the number of proliferating CFU-S-12 and pre-CFU-S in recipient bone marrow increased in comparison with the control. In case of an allogeneic donor-recipient combination, the number of 9-day splenic colonies in thymodepressin-treated animals increased 5-fold compared to the control. We hypothesized that thymodepressin therapy and irradiation form an adaptive response of the recipient hemopoietic microenvironment in the bone marrow, which provides conditions for optimal functioning of donor hemopoietic precursors.

[The protective effect of thermal treatment before irradiation on CFUs from bone marrow of mice: a potential involvement of heat shock proteins]. / Zashchitnyi éffekt predradiatsionnoi termicheskoi obrabotki myshei na KOEs ikh kostnogo mozga: vozmozhnoe uchastie belkov teplovogo shoka.

It was studied on mice how prior whole body hyperthemia affects a colony-forming ability of bone marrow after gamma-irradiation. It was found that heating of the animals (42 degrees C, 10 min) 18-22 h before their total irradiation (4 Gy) increases 2-fold the level of CFUs8 and CFUs12 determined in the spleen exotest. The induced radioresistance correlated with accumulation of heat shock proteins, HSP70 and HSP25, in tissues of preheated mice. Injection of quercetin (a selective inhibitor of the heat shock protein synthesis) 0.5 h before the heating fully abolished both the subsequent heat shock protein accumulation and the rise in CFUs populations as compared with control. It is suggested that heat shock proteins, whose expression increases in response to hyperthermia, can play a role of endogenous radioprotectors. Possible mechanisms of their protective action under irradiation are discussed.

[The synthetic peptide D-(iEW) (thymodepressin) protects bone marrow CFU-S from ionizing radiation exposure]. / Sinteticheskii peptid D-(iEW) (timodepressin) zashchishchaet KOEs kostnogo mozga ot vozdeistviia ioniziruiushchei radiatsii.

Synthetic polypeptide D-(iEW) (Thymodepressin) was shown to reduce the bone marrow CFU-S in the S-phase of cell cycle. Apparently, due to this property the agent being administered 2 days before the irradiation with 4 Gy causes a prominent restoration of the CFU-S population afterwards. The 3-5 times higher value of this parameter as compared to the control (irradiation only) is likely to be connected to the increased "survival" of the Thymodepressin-protected CFU-S after the treatment with ionizing irradiation.

[Radiation damage of the thyroid gland as a probable cause of increased incidence of non-Hodgkin lymphoma and other hematologic diseases]. / Radiatsionnoe povrezhdenie shchitovidnoi zhelezy kak veroiatnaia prichina uvelicheniia chastoty nekhodzhkinskikh limfom i drugikh gematologicheskikh zabolevanii.

Th distribution of autoimmune thyroiditis in the patients with diseases of blood system was investigate. The attribute of autoimmune thyroiditis was revealed by the detection of antimicrosomal antibodies. It was established that the autoimmune thyroiditis are more often in patients with various hematological diseases than in control group. It is supposed that the increase in frequency of some hematological diseases in residents suffered from the Chernobyl accident can be defined not only by the influence of the radiation on blood system, but also can be connected with damage to thyroid glands.

[Natural antihistone antibodies: their possible role in genesis of radiation injuries]. / Prirodnye antigistonovye antitela: veroiatnaia rol' v geneze luchevykh porazhenii.

The substantial decrease of free natural antibodies reacting with histones under effect of 4 Gy total dose radiation in sera of healthy animals was established. It is supposed that the binding of natural antihistone antibodies with histones reduces the cytotoxic reactivity of histones released from cells under effect of ionizing radiation. It was determined that complexes of antihistone antibodies and histones get the ability to react with DNA. The possible biological role of these complexes is discussed.

[A comparative study of the immunological (antimicrosomal antibodies) and cytogenetic indices of children living in radioactively contaminated areas of Bryansk Province]. / Sravnitel'noe izuchenie immunologicheskikh (antimikrosomal'nykh antitel) i tsitogeneticheskikh pokazatelei u detei, prozhivaiushchikh na radioaktivno zagriaznennykh territoriakh Brianskoi oblasti.

The chromosome dicentric aberrations in the lymphocytes and levels of antibodies to human thyroid microsomal antigen in the serum of the children lived in the area of Bryansk Province suffered from the Chernobyl accident was examined. Correlation between those tests was not estimated: the autoantibodies were revealed in group with dicentrics and without those in 4.0% and 4.5% of cases correspondingly. Antimicrosomal antibodies were revealed more frequently (5.0%) and in higher titers in the children from the more polluted Bryansk Province than in those from Kaluga Province (3.1%). These data can testify about the role of inside radiation of thyroid gland in appearance of autoimmune thyroiditis signs.

[Palladium-containing compounds possess radiation-protective properties and enhance adaptive response of CFUs to radiation]. / Palladiisoderzhashchie soedineniia obladaiut radioprotektivnymi svoistvami i usilivaiut adaptivnyi otvek KOEs k radiatsionnomu vozdeistviiu.

Efasol and potassium tetrachloropalladate being injected into mice prior to irradiation with a dose of 150 sGy increase the colony forming activity of animals bone marrow cells transplanted in lethally irradiated recipient mice. The palladium presence in such compounds is supposed to be a reason of radioprotection. Upon the combined application of potassium tetrachloropalladate and irradiation of mice with an adaptive dose of 5 sGy a potentiation of individual effect of each separate agent to increase CFUs resistance against the consequent irradiation was observed.

[The probable sequelae of thyroid damage from radioactive iodine during the Chernobyl accident]. / Veroiatnye posledstviia povrezhdeniia radioaktivnym iodom shchitovidnoi zhelezy v period Chernobyl'skoi avarii.

An important peculiarity of the Chernobyl catastrophe is the discharge into the atmosphere of tremendous amount of radioactive iodine and, as a result, selective damage of the thyroid in children from the affected areas. The most dangerous consequence is the thyroid cancer. The analysis of the situation when children's thyroids were subjected to irradiation shows that tumors can most frequently develop as late as 20-30 years after irradiation. There are reasons to believe that tumors are induced by low dose of irradiation. The most important factor in development of pathologies is for sure the age of the children of the moment of irradiation. A well-known consequence of the impact of radiation on the thyroid is the lymphocyte thyroiditis. The interest to this pathology is determined by the fact that it substantially increases the probability of development of various haematologic diseases (lympho- and myeloproliferative neoplasms).