RESUMEN
Plastic and mixed plastic waste (PW) has received increased worldwide attention owing to its huge rate of production, high persistency in the environment, and unsustainable waste management practices. Therefore, sustainable PW management and upcycling approaches are imperative to achieve the objectives of the United Nations Sustainable Development Goals. Numerous recent studies have shown the application and feasibility of various PW conversion techniques to produce materials with better economic value. Within this framework, the current review provides an in-depth analysis of cutting-edge thermochemical technologies such as pyrolysis, gasification, carbonization, and photocatalysis that can be used to value plastic and mixed PW in order to produce energy and industrial chemicals. Additionally, a thorough examination of the environmental impacts of contemporary PW upcycling techniques and their commercial feasibility through life cycle assessment (LCA) and techno-economical assessment are provided in this review. Finally, this review emphasizes the opportunities and challenges accompanying with existing PW upcycling techniques and deliver recommendations for future research works.
RESUMEN
Neurons and sensory cells are particularly vulnerable to oxidative stress due to their high oxygen demand during stimulus perception and transmission. The mechanisms that protect them from stress-induced death and degeneration remain elusive. Here we show that embryonic deletion of the chromodomain helicase DNA-binding protein 7 (CHD7) in auditory neurons or hair cells leads to sensorineural hearing loss due to postnatal degeneration of both cell types. Mechanistically, we demonstrate that CHD7 controls the expression of major stress pathway components. In its absence, hair cells are hypersensitive, dying rapidly after brief exposure to stress inducers, suggesting that sound at the onset of hearing triggers their degeneration. In humans, CHD7 haploinsufficiency causes CHARGE syndrome, a disorder affecting multiple organs including the ear. Our findings suggest that CHD7 mutations cause developmentally silent phenotypes that predispose cells to postnatal degeneration due to a failure of protective mechanisms.
Asunto(s)
Nervio Coclear/fisiopatología , Proteínas de Unión al ADN/genética , Células Ciliadas Auditivas/fisiología , Mutación , Fenotipo , Estrés Fisiológico , Animales , Proteínas de Unión al ADN/metabolismo , Femenino , Humanos , Masculino , RatonesRESUMEN
Plastic is referred to as a "material of every application". From the packaging and automotive industries to the medical apparatus and computer electronics sectors, plastic materials are fulfilling demands efficiently. These plastics usually end up in landfills and incinerators, creating plastic waste pollution. According to the Environmental Protection Agency (EPA), in 2015, 9.1% of the plastic materials generated in the U.S. municipal solid waste stream was recycled, 15.5% was combusted for energy, and 75.4% was sent to landfills. If we can produce high-value chemicals from plastic wastes, a range of various product portfolios can be created. This will help to transform chemical industries, especially the petrochemical and plastic sectors. In turn, we can manage plastic waste pollution, reduce the consumption of virgin petroleum, and protect human health and the environment. This review provides a description of chemicals that can be produced from different plastic wastes and the research challenges involved in plastic waste to chemical production. This review also provides a brief overview of the state-of-the-art processes to help future system designers in the plastic waste to chemicals area.
RESUMEN
During vertebrate embryonic development, the formation of axial structures is driven by a population of stem-like cells that reside in a region of the tailbud called the chordoneural hinge (CNH). We have compared the mouse CNH transcriptome with those of surrounding tissues and shown that the CNH and tailbud mesoderm are transcriptionally similar, and distinct from the presomitic mesoderm. Amongst CNH-enriched genes are several that are required for axial elongation, including Wnt3a, Cdx2, Brachyury/T and Fgf8, and androgen/oestrogen receptor nuclear signalling components such as Greb1 We show that the pattern and duration of tailbud Greb1 expression is conserved in mouse, zebrafish and chicken embryos, and that Greb1 is required for axial elongation and somitogenesis in zebrafish embryos. The axial truncation phenotype of Greb1 morphant embryos can be explained by much reduced expression of No tail (Ntl/Brachyury), which is required for axial progenitor maintenance. Posterior segmentation defects in the morphants (including misexpression of genes such as mespb, myoD and papC) appear to result, in part, from lost expression of the segmentation clock gene, her7.
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Desarrollo Embrionario/genética , Proteínas de la Membrana/genética , Morfogénesis/genética , Proteínas de Neoplasias/genética , Proteínas de Pez Cebra/genética , Animales , Biología Computacional/métodos , Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Técnicas de Silenciamiento del Gen , Ratones , Fenotipo , Células Madre/citología , Células Madre/metabolismo , TranscriptomaRESUMEN
During early embryogenesis, the ectoderm is rapidly subdivided into neural, neural crest and sensory progenitors. How the onset of lineage determinants and the loss of pluripotency markers are temporally and spatially coordinated in vivo is still debated. Here, we identify a crucial role for the transcription factor PRDM1 in the orderly transition from epiblast to defined neural lineages in chick. PRDM1 is initially expressed broadly in the entire epiblast, but becomes gradually restricted as cell fates are specified. We find that PRDM1 is required for the loss of some pluripotency markers and the onset of neural, neural crest and sensory progenitor specifier genes. PRDM1 directly activates their expression by binding to their promoter regions and recruiting the histone demethylase Kdm4a to remove repressive histone marks. However, once neural lineage determinants become expressed, they in turn repress PRDM1, whereas prolonged PRDM1 expression inhibits neural, neural crest and sensory progenitor genes, suggesting that its downregulation is necessary for cells to maintain their identity. Therefore, PRDM1 plays multiple roles during ectodermal cell fate allocation.
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Diferenciación Celular/genética , Sistema Nervioso/embriología , Cresta Neural/embriología , Células-Madre Neurales/fisiología , Factor 1 de Unión al Dominio 1 de Regulación Positiva/fisiología , Células Receptoras Sensoriales/fisiología , Animales , Animales Modificados Genéticamente , Embrión de Pollo , Desarrollo Embrionario/genética , Regulación del Desarrollo de la Expresión Génica , Sistema Nervioso/citología , Cresta Neural/citología , Neurogénesis/genética , Células Receptoras Sensoriales/citologíaRESUMEN
Around the time of gastrulation in higher vertebrate embryos, inductive interactions direct cells to form central nervous system (neural plate) or sensory placodes. Grafts of different tissues into the periphery of a chicken embryo elicit different responses: Hensen's node induces a neural plate whereas the head mesoderm induces placodes. How different are these processes? Transcriptome analysis in time course reveals that both processes start by induction of a common set of genes, which later diverge. These genes are remarkably similar to those induced by an extraembryonic tissue, the hypoblast, and are normally expressed in the pregastrulation stage epiblast. Explants of this epiblast grown in the absence of further signals develop as neural plate border derivatives and eventually express lens markers. We designate this state as "preborder"; its transcriptome resembles embryonic stem cells. Finally, using sequential transplantation experiments, we show that the node, head mesoderm, and hypoblast are interchangeable to begin any of these inductions while the final outcome depends on the tissue emitting the later signals.
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Sistema Nervioso Central/metabolismo , Inducción Embrionaria , Células Madre Embrionarias/metabolismo , Gástrula/metabolismo , Mesodermo/metabolismo , Placa Neural/metabolismo , Animales , Sistema Nervioso Central/embriología , Embrión de Pollo , Gástrula/embriología , Perfilación de la Expresión Génica/métodos , Regulación del Desarrollo de la Expresión Génica , Hibridación in Situ , Mesodermo/embriología , Placa Neural/embriologíaRESUMEN
In vertebrates, cranial placodes contribute to all sense organs and sensory ganglia and arise from a common pool of Six1/Eya2+ progenitors. Here we dissect the events that specify ectodermal cells as placode progenitors using newly identified genes upstream of the Six/Eya complex. We show in chick that two different tissues, namely the lateral head mesoderm and the prechordal mesendoderm, gradually induce placode progenitors: cells pass through successive transcriptional states, each identified by distinct factors and controlled by different signals. Both tissues initiate a common transcriptional state but over time impart regional character, with the acquisition of anterior identity dependent on Shh signalling. Using a network inference approach we predict the regulatory relationships among newly identified transcription factors and verify predicted links in knockdown experiments. Based on this analysis we propose a new model for placode progenitor induction, in which the initial induction of a generic transcriptional state precedes regional divergence.
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Transducción de Señal/fisiología , Vertebrados/embriología , Animales , Comunicación Celular/genética , Comunicación Celular/fisiología , Embrión de Pollo , Pollos , Ectodermo/citología , Ectodermo/embriología , Ectodermo/metabolismo , Electroporación , Ganglios Sensoriales/citología , Ganglios Sensoriales/embriología , Ganglios Sensoriales/metabolismo , Regulación del Desarrollo de la Expresión Génica/genética , Regulación del Desarrollo de la Expresión Génica/fisiología , Hibridación in Situ , Análisis de Secuencia por Matrices de Oligonucleótidos , Codorniz , Órganos de los Sentidos/citología , Órganos de los Sentidos/embriología , Órganos de los Sentidos/metabolismo , Transducción de Señal/genética , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Vertebrados/metabolismoRESUMEN
The signal peptide plays a key role in targeting and membrane insertion of secretory and membrane proteins in both prokaryotes and eukaryotes. In E. coli, recombinant proteins can be targeted to the periplasmic space by fusing naturally occurring signal sequences to their N-terminus. The model protein thioredoxin was fused at its N-terminus with malE and pelB signal sequences. While WT and the pelB fusion are soluble when expressed, the malE fusion was targeted to inclusion bodies and was refolded in vitro to yield a monomeric product with identical secondary structure to WT thioredoxin. The purified recombinant proteins were studied with respect to their thermodynamic stability, aggregation propensity and activity, and compared with wild type thioredoxin, without a signal sequence. The presence of signal sequences leads to thermodynamic destabilization, reduces the activity and increases the aggregation propensity, with malE having much larger effects than pelB. These studies show that besides acting as address labels, signal sequences can modulate protein stability and aggregation in a sequence dependent manner.
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Escherichia coli/metabolismo , Pliegue de Proteína , Señales de Clasificación de Proteína , Tiorredoxinas/química , Tiorredoxinas/metabolismo , Secuencia de Aminoácidos , Naftalenosulfonatos de Anilina/metabolismo , Tampones (Química) , Rastreo Diferencial de Calorimetría , Cromatografía en Gel , Dicroismo Circular , Electroforesis en Gel de Poliacrilamida , Guanidina/farmacología , Insulina/metabolismo , Datos de Secuencia Molecular , Desnaturalización Proteica/efectos de los fármacos , Pliegue de Proteína/efectos de los fármacos , Replegamiento Proteico/efectos de los fármacos , Estabilidad Proteica/efectos de los fármacos , Estructura Cuaternaria de Proteína , Proteolisis/efectos de los fármacos , Proteínas Recombinantes de Fusión/metabolismo , Espectrometría de Fluorescencia , TemperaturaRESUMEN
Molten globule-like intermediates have been shown to occur during protein folding and are thought to be involved in protein translocation and membrane insertion. However, the determinants of molten globule stability and the extent of specific packing in molten globules is currently unclear. Using far- and near-UV CD and intrinsic and ANS fluorescence, we show that four periplasmic binding proteins (LBP, LIVBP, MBP, and RBP) form molten globules at acidic pH values ranging from 3.0 to 3.4. Only two of these (LBP and LIVBP) have similar sequences, but all four proteins adopt similar three-dimensional structures. We found that each of the four molten globules binds to its corresponding ligand without conversion to the native state. Ligand binding affinity measured by isothermal titration calorimetry for the molten globule state of LIVBP was found to be comparable to that of the corresponding native state, whereas for LBP, MBP, and RBP, the molten globules bound ligand with approximately 5-30-fold lower affinity than the corresponding native states. All four molten globule states exhibited cooperative thermal unfolding assayed by DSC. Estimated values of DeltaCp of unfolding show that these molten globule states contain 28-67% of buried surface area relative to the native states. The data suggest that molten globules of these periplasmic binding proteins retain a considerable degree of long range order. The ability of these sequentially unrelated proteins to form highly ordered molten globules may be related to their large size as well as an intrinsic property of periplasmic binding protein folds.
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Escherichia coli/metabolismo , Proteínas de Unión Periplasmáticas/química , Proteínas de Unión Periplasmáticas/metabolismo , Rastreo Diferencial de Calorimetría , Proteínas Portadoras/química , Proteínas Portadoras/metabolismo , Dicroismo Circular , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/metabolismo , Concentración de Iones de Hidrógeno , Ligandos , Conformación Proteica , Pliegue de Proteína , Espectrometría de Fluorescencia , TermodinámicaRESUMEN
The amino acid Pro is more rigid than other naturally occurring amino acids and, in proteins, lacks an amide hydrogen. To understand the structural and thermodynamic effects of Pro substitutions, it was introduced at 13 different positions in four different proteins, leucine-isoleucine-valine binding protein, maltose binding protein, ribose binding protein, and thioredoxin. Three of the maltose binding protein mutants were characterized by X-ray crystallography to confirm that no structural changes had occurred upon mutation. In the remaining cases, fluorescence and CD spectroscopy were used to show the absence of structural change. Stabilities of wild type and mutant proteins were characterized by chemical denaturation at neutral pH and by differential scanning calorimetry as a function of pH. The mutants did not show enhanced stability with respect to chemical denaturation at room temperature. However, 6 of the 13 single mutants showed a small but significant increase in the free energy of thermal unfolding in the range of 0.3-2.4 kcal/mol, 2 mutants showed no change, and 5 were destabilized. In five of the six cases, the stabilization was because of reduced entropy of unfolding. However, the magnitude of the reduction in entropy of unfolding was typically several fold larger than the theoretical estimate of -4 cal K(-1) mol(-1) derived from the relative areas in the Ramachandran map accessible to Pro and Ala residues, respectively. Two double mutants were constructed. In both cases, the effects of the single mutations on the free energy of thermal unfolding were nonadditive.
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Proteínas Portadoras/química , Proteínas de Escherichia coli/química , Proteínas de Unión Periplasmáticas/química , Prolina/química , Desnaturalización Proteica , Tiorredoxinas/química , Sustitución de Aminoácidos , Proteínas Portadoras/genética , Dicroismo Circular , Cristalografía por Rayos X , Entropía , Escherichia coli/química , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Modelos Moleculares , Mutagénesis Sitio-Dirigida , Mutación , Proteínas de Unión Periplasmáticas/genética , Conformación Proteica , Desnaturalización Proteica/efectos de los fármacos , Pliegue de Proteína , Proteínas Recombinantes de Fusión/química , Espectrometría de Fluorescencia , Relación Estructura-Actividad , Temperatura , Termodinámica , Tiorredoxinas/genéticaRESUMEN
Calculations predict that cation- interactions make an important contribution to protein stability. While there have been some attempts to experimentally measure strengths of cation-pi interactions using peptide model systems, much less experimental data are available for globular proteins. We have attempted to determine the magnitude of cation-pi interactions of Lys with aromatic amino acids in four different proteins (LIVBP, MBP, RBP, and Trx). In each case, Lys was replaced with Gln and Met. In a separate series of experiments, the aromatic amino acid in each cation-pi pair was replaced by Leu. Stabilities of wild-type (WT) and mutant proteins were characterized by both thermal and chemical denaturation. Gln and aromatic --> Leu mutants were consistently less stable than corresponding Met mutants, reflecting the nonisosteric nature of these substitutions. The strength of the cation-pi interaction was assessed by the value of the change in the free energy of unfolding [DeltaDeltaG(degrees) = DeltaG(degrees)(Met) - DeltaG(degrees)(WT)]. This ranged from +1.1 to -1.9 kcal/mol (average value -0.4 kcal/mol) at 298 K and +0.7 to -2.6 kcal/mol (average value -1.1 kcal/mol) at the Tm of each WT. It therefore appears that the strength of cation-pi interactions increases with temperature. In addition, the experimentally measured values are appreciably smaller in magnitude than calculated values with an average difference /DeltaG(degrees)expt - DeltaG(degrees)calc/av of 2.9 kcal/mol. At room temperature, the data indicate that cation-pi interactions are at best weakly stabilizing and in some cases are clearly destabilizing. However, at elevated temperatures, close to typical Tm's, cation-pi interactions are generally stabilizing.
Asunto(s)
Cationes/química , Proteínas de Escherichia coli/química , Lisina/química , Modelos Moleculares , Proteínas Periplasmáticas/química , Pliegue de Proteína , Sustitución de Aminoácidos , Escherichia coli/química , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Lisina/genética , Proteínas Periplasmáticas/genética , Estructura Secundaria de Proteína , Electricidad EstáticaRESUMEN
Disulfides cross-link residues in a protein that are separated in primary sequence and stabilize the protein through entropic destabilization of the unfolded state. While the removal of naturally occurring disulfides leads to protein destabilization, introduction of engineered disulfides does not always lead to significant stabilization of a protein. We have analyzed naturally occurring disulfides that span adjacent antiparallel strands of beta sheets (cross-strand disulfides). Cross-strand disulfides have recently been implicated as redox-based conformational switches in proteins such as gp120 and CD4. The propensity of these disulfides to act as conformational switches was postulated on the basis of the hypothesis that this class of disulfide is conformationally strained. In the present analysis, there was no evidence to suggest that cross-strand disulfides are more strained compared to other disulfides as assessed by their torsional energy. It was also observed that these disulfides occur solely at non-hydrogen-bonded (NHB) registered pairs of adjacent antiparallel strands and not at hydrogen-bonded (HB) positions as suggested previously. One of the half-cystines involved in cross-strand disulfide formation often occurs at an edge strand. Experimental confirmation of the stabilizing effects of such disulfides was carried out in Escherichia coli thioredoxin. Four pairs of cross-strand cysteines were introduced, two at HB and two at NHB pairs. Disulfides were formed in all four cases. However, as predicted from our analysis, disulfides at NHB positions resulted in an increase in melting temperature of 7-10 degrees C, while at HB positions there was a corresponding decrease of -7 degrees C. The reduced state of all proteins had similar stability.