Asunto(s)
Compuestos de Bencidrilo/metabolismo , Hepatocitos/metabolismo , Resistencia a la Insulina/fisiología , Fenoles/metabolismo , Proteína 3 Supresora de la Señalización de Citocinas/genética , Proteína 3 Supresora de la Señalización de Citocinas/metabolismo , Animales , Regulación de la Expresión Génica , Técnicas de Silenciamiento del Gen , Glucosa/metabolismo , Células Hep G2/citología , Células Hep G2/metabolismo , Humanos , Insulina/metabolismo , Proteínas Sustrato del Receptor de Insulina/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismoRESUMEN
The aim of the present study was to investigate whether environmental endocrine disrupting chemical, bisphenol A (BPA), affects secretion of suppressor of cytokine signaling 3 (SOCS-3) and insulin signaling transduction in 3T3-L1 adipocytes. 3T3-L1 adipocytes were treated for 0, 2, 6, 12 and 24 h with BPA at 80 µM in serumdeprived medium. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to detect the mRNA expression levels of SOCS3 and protein expression levels of SOCS3, insulin receptor substrate 1 (IRS1), phosphorylated (p)IRS1, Akt and pAkt. The levels of pIRS1, Akt and pAkt in cultures treated for 6 h with BPA were also analyzed by immunofluorescence. The SOCS3 mRNA and protein expression levels were decreased in the 6, 12 and 24 h groups. The levels of pIRS1 and pAkt protein were markedly downregulated, while the level of IRS1 and Akt protein remained unaltered among these groups, which was consistent with the results observed using immunofluorescence. BPA may inhibit insulin signal transduction and result in the occurrence of insulin resistance via promoting the expression of SOCS-3.