RESUMEN
Objective: To evaluate the hemostatic efficacy, safety and immunogenicity of recombinant human thrombin in the treatment of liver wounds that still ooze after conventional surgical hemostasis. Methods: A multicenter, stratified randomized, double-blind, placebo-controlled phase â ¢ trial with a planned enrollment of 510 subjects at 33 centers, with a 2â¶1 randomization to the thrombin group versus the placebo group. An interim analysis will be conducted after approximately 70% of the subjects have completed the observation period. The primary efficacy endpoint was the rate of hemostasis within 6 minutes at the point of bleeding that could be evaluated. Safety analysis was performed one month after surgery, and the positive rates of anti-drug antibody (ADA) and neutralizing antibody were evaluated. Results: At the interim analysis, a total of 348 subjects had been randomized and received the study drug (215 were male and 133 were female). They were aged 19-69 (52.9±10.9)years. Among them, 232 were in the thrombin group and 116 were in the placebo group, with balanced and comparable demographics and baseline characteristics between the two groups. The hemostasis rate at 6 minutes was 71.6% (95%CI:65.75%-77.36%) in the thrombin group and 44.0% (95%CI: 34.93%-53.00%) in the placebo group, respectively (P<0.001). No grade≥3 drug-related adverse events and no drug-related deaths were reported from the study.No recombinant human thrombin-induced immunologically-enhanced ADA or immunologically-induced ADA was detected after topical use in subjects. Conclusion: Recombinant human thrombin has shown significant hemostatic efficacy and good safety in controlling bleeding during liver resection surgery, while also demonstrating low immunogenicity characteristics.
Asunto(s)
Hemostáticos , Trombina , Humanos , Masculino , Femenino , Trombina/efectos adversos , Hemostáticos/uso terapéutico , Hemostáticos/efectos adversos , Hígado , Hemostasis , Resultado del TratamientoRESUMEN
OBJECTIVE: The present study aimed to explore the transmission pattern and the incubation period of coronavirus disease 2019 (COVID-19) as well as the clinical characteristics of infants with COVID-19 to provide a scientific basis for introducing further measures to reduce the infection rate and control the pandemic. PATIENTS AND METHODS: A descriptive epidemiological study of 18 patients with COVID-19 in People's Hospital of Deyuan was carried out. Among these patients, 16 cases were connected with clusters (11 family-cluster cases and 5 public-cluster cases). The basic characteristics, clinical symptoms, and epidemiological characteristics of the patients were considered in the investigation. RESULTS: The median age of the 18 patients was 44.5 years (37.5-52.0 years), and there were 10 males and 8 females in the sample. The main clinical symptoms were fever and cough. The epidemiological characteristics were as follows: (1) the median incubation period was 8 days (with an interquartile range of 4-12 days); (2) the incubation period in one case was ≥18 days; (3) one infant patient was asymptomatic prior to their diagnosis; and (4) two asymptomatic patients had a positive nucleic acid test after their family members were diagnosed with COVID-19. CONCLUSIONS: COVID-19 can be transmitted in many ways, including via respiratory droplets and indirect contact, and it spreads easily among close contacts. People with a history of contact with areas affected by the disease should be isolated at home for 14 days. Moreover, attention should be focused on the issues of asymptomatic infectors, asymptomatic infants, and infants with mild symptoms.
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COVID-19/epidemiología , Punto Alto de Contagio de Enfermedades , Periodo de Incubación de Enfermedades Infecciosas , Adolescente , Adulto , Anciano , Infecciones Asintomáticas , COVID-19/fisiopatología , COVID-19/transmisión , Niño , Preescolar , China/epidemiología , Transmisión de Enfermedad Infecciosa , Femenino , Humanos , Lactante , Masculino , Persona de Mediana Edad , SARS-CoV-2 , Adulto JovenRESUMEN
Objective: To analyze the outcome and the prognostic factors of hepatic veno-occlusive disease (HVOD) after hematopoietic stem cell transplantation (HSCT). Methods: A total of 797 patients receiving HSCT were analyzed retrospectively. The prophylaxis regimen of HVOD in the First Affiliated Hospital of Guangxi Medical University consisted of low molecular weight heparin and lipoprostaglandin E1 (PGE1). Results: Fifty-nine patients (7.4%) developed HVOD at 3-49 days after HSCT (median 12 days). Age younger than 15 years at transplant(HR= 6.47, P<0.001), busulphan conditioning (HR=6.40, P<0.001), thalassemia major (HR=6.35, P<0.001), allogeneic transplantation (HR=7.74, P=0.005) were univariate risk factors for HVOD. Multivariate analyses suggested that thalassemia major and busulphan conditioning were independently correlated with the development of HVOD. Conclusion: Thalassemia major and busulphan conditioning are independent risk factors for HVOD after HSCT.
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Trasplante de Células Madre Hematopoyéticas/efectos adversos , Heparina de Bajo-Peso-Molecular/uso terapéutico , Enfermedad Veno-Oclusiva Hepática/inducido químicamente , Enfermedad Veno-Oclusiva Hepática/prevención & control , Acondicionamiento Pretrasplante/métodos , Adolescente , Busulfano/administración & dosificación , Busulfano/efectos adversos , China/epidemiología , Enfermedad Veno-Oclusiva Hepática/epidemiología , Humanos , Inmunosupresores/administración & dosificación , Inmunosupresores/efectos adversos , Complicaciones Posoperatorias , Estudios Retrospectivos , Factores de Riesgo , Acondicionamiento Pretrasplante/efectos adversos , Trasplante Homólogo , Resultado del TratamientoRESUMEN
Liver cancer is one of the most common cancers in the world. Macrothele raven venom, a complicated mixture of neurotoxic peptides, proteins and low molecular weight material, has antitumor properties, but its mechanism of action is unknown. Moderate exercise has been shown to shrink tumors and cause a remarkable reduction in the tumor growth rate. In this study, we examined the antitumor effect of Macrothele raven venom in combination with exercise on tumor-bearing mice. Our results demonstrate that aerobic exercise in combination with venom administered at different doses was much more effective in a mouse H22 hepatoma model compared to separate administration of the 2 treatments. The underlying mechanism of this effect may be related to the expression of various tumor suppressor factors.
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Antineoplásicos/administración & dosificación , Terapia por Ejercicio , Neoplasias Hepáticas Experimentales/terapia , Condicionamiento Físico Animal , Venenos de Araña/administración & dosificación , Animales , Biomarcadores de Tumor/metabolismo , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Inmunohistoquímica , Infusiones Intravenosas , Neoplasias Hepáticas Experimentales/metabolismo , Neoplasias Hepáticas Experimentales/patología , Masculino , Ratones , Necrosis , Carga TumoralRESUMEN
A cytosolic manganese superoxide dismutase gene (Es-cMnSOD) was cloned from the Chinese mitten crab Eriocheir sinensis, using reverse transcription-polymerase chain reaction and the rapid amplification of cDNA ends. The open reading frame of Es-cMnSOD is 867 bp in length and encodes a 288-amino acid protein without a signal peptide. The calculated molecular mass of the translated protein of Es-cMnSOD is 31.43 kDa, with an estimated isoelectric point of 6.30. The deduced amino acid sequence of Es-cMnSOD has similarities of 90, 89, 84, 87, and 81% to those of white shrimp Litopenaeus vannamei MnSOD, black tiger shrimp Penaeus monodon MnSOD, giant freshwater prawn Macrobrachium rosenbergii MnSOD, blue crab Callinectes sapidus MnSOD, and red swamp crayfish Procambarus clarkii MnSOD, respectively. Es-cMnSOD contains a manganese superoxide dismutase domain (DVWEHAYY) and 4 conserved amino acids responsible for binding manganese. Es-cMnSOD was expressed in the hemocytes, eyestalk, muscle, intestine, gill, and hepatopancreas. Es-cMnSOD transcripts in hemocytes of E. sinensis increased at 1.5 and 48 h after injection of Aeromonas hydrophila, indicating that the induction of the SOD system response occurred within a short period of time. This study suggests that MnSOD may play a critical role in crab immunity, allowing efficient activation of an early innate immune response in the crab.
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Braquiuros/enzimología , Citosol/enzimología , Superóxido Dismutasa/genética , Secuencias de Aminoácidos , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN Complementario/genética , Regulación Enzimológica de la Expresión Génica , Datos de Secuencia Molecular , Filogenia , ARN Mensajero/genética , ARN Mensajero/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Superóxido Dismutasa/química , Superóxido Dismutasa/metabolismoRESUMEN
The scaly-sided merganser (Mergus squamatus), found in temperate East Asia, has been reduced to a very small population. Central and southern China are its main wintering habitat. However, populations have declined greatly since the 1980s due to habitat loss and degradation, and poaching. To meet the urgent need for up-to-date conservation information, we examined RAPD DNA markers from 156 specimens in 6 populations in Jiangxi Province. We found that genetic diversity (based on individual similarities) is in fact low; molecular variance between populations ranged from 0.137 to 0.347. Genetic similarity ranged from 0.683 to 0.866. In conclusion, the geographical pattern of genetic diversity supports the long-term refugial status of the scaly-sided merganser in central-southern China; strong conservation measures should be taken to maintain the merganser in this region.
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Anseriformes/genética , Marcadores Genéticos/genética , Variación Genética , Técnica del ADN Polimorfo Amplificado Aleatorio , Animales , China , Ecosistema , Especies en Peligro de Extinción , Genética de PoblaciónRESUMEN
Obstructive jaundice is a common surgical disease and a variety of end-stage severe liver injuries still lack effective treatments. Compared to traditional liver transplantation therapy, herbal treatment is noninvasive and has fewer side effects. Research results have shown that a modified major decoction of bupleurum can reduce the toxic reaction of obstructive jaundice, even though the mechanism is unclear. A period of chronic exercise training can significantly reduce TLR4 expression in mononuclear cells and the secretion of inflammatory cell factors. Our study administered a modified major decoction of bupleurum in combination with exercise in rats with obstructive jaundice and the results indicated that applying a major bupleurum decoction in combination with moderately intense aerobic exercise showed a beneficial effect on adjusting the expression of liver inflammatory cytokines, which thus improved immunity and finally reduced the liver injury of rats with obstructive jaundice.
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Bupleurum/química , Ictericia Obstructiva/terapia , Condicionamiento Físico Animal/métodos , Receptor Toll-Like 4/metabolismo , Animales , Terapia Combinada , Citocinas/metabolismo , Modelos Animales de Enfermedad , Inflamación/fisiopatología , Inflamación/terapia , Ictericia Obstructiva/fisiopatología , Hepatopatías/fisiopatología , Hepatopatías/terapia , Masculino , Extractos Vegetales/farmacología , Ratas , Ratas Sprague-Dawley , Índice de Severidad de la Enfermedad , Transducción de SeñalRESUMEN
The preparation of an unsymmetrical binucleating ligand bearing a bridging oxadiazole ring flanked on one side by three ligands and on the other by four ligands is described. When bound to two metals, the ligand forms complexes where the metals are in 5- and 6-coordinate sites after the incorporation of an exogenous bridging ligand. A di-Co(2+) complex of this ligand has been prepared containing a hydroxide bridge. The complex is readily oxidized to the di-Co(3+) state by outer sphere electron transfer with ferrocenium ions. Addition of Br(2) or NO(2)(+) to the di-Co(2+) complex leads to the rapid formation of the di-Co(3+) bromo or nitro complexes, respectively. The ligand characteristics which allow for double oxidation with ferrocenium ions and for the one-site addition two-metal oxidations with Br(2) and NO(2)(+) are discussed in terms of mechanical coupling between the two metal sites.
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Cobalto/química , Sitios de Unión , Ligandos , Oxidación-Reducción , Análisis EspectralRESUMEN
Two (1,3,4)-oxadiazole ligands have been prepared. In one case the oxadiazole ring is flanked by two o-aniline groups, and in the other case it is an extension of the first where the amines are condensed with 2-picolyl groups. A monometallic copper(II) complex of the former has been prepared, and its crystal structure was determined. A number of bimetallic copper(II), cobalt(II), and nickel(II) complexes of the di-deprotonated latter ligand were prepared and isolated. The crystal structure of the cobalt(II) complex bearing two acetate bridges is reported. The work demonstrates that the seldom-employed oxadiazole ring can be used effectively for generating bimetallic complexes.
Asunto(s)
Compuestos Organometálicos/química , Oxadiazoles/química , Aminas/química , Compuestos de Anilina/química , Cobalto/química , Cobre/química , Ligandos , Estructura Molecular , Níquel/químicaRESUMEN
Four binucleating ligands bearing 4- and 6-coordinate sites employing phenolate bridges have been prepared. Bimetallic copper(II) and nickel(II) complexes of some of these ligands have been isolated and characterized. Crystal structures of two of the copper(II) complexes have been determined. A monometallic manganese(II) complex of one of these ligands was isolated. Upon exposure to dioxygen, acetonitrile solutions of the complex in the presence of chloride ions lead to the formation of a manganese(IV) complex. The crystal structure of this complex is reported, and it is shown that the metal is in the 4-coordinate ligand site and is bound to two chloride ions.