Edwardsiella tarda Causing Septicemia in a Wild Crested Ibis (Nipponia nippon).

An adult Crested Ibis (Nipponia nippon) was found moribund in the Qinling area of China. Postmortem examination and histopathological analysis revealed lung inflammation and multi-organ hemorrhage. Bacterial isolation and whole-genome sequencing confirmed Edwardsiella tarda infection.

The Size of Winter-Flooded Paddy Fields No Longer Limits the Foraging Habitat Use of the Endangered Crested Ibis (Nipponia nippon) in Winter.

Paddy fields have traditionally been viewed as the key foraging habitats for the endangered crested ibis (Nipponia nippon). With the population of this species now increasing, its distribution has expanded to both lowland areas and outside the nature reserve. However, little is known about the current foraging habitat preferences of these birds, especially during winter. In this research, a total of 54 used sites and 50 unused sites were investigated during winter from December 2011 to January 2012. The results of logistic regression analysis indicate that soil softness, human disturbance, and distance to the nearest road were important factors. For the site plots of winter-flooded paddy fields, the birds prefer the paddy fields with higher coverage of vegetation, except softer foraging sites and lower human-related disturbance. In lowland areas, the size of winter-flooded paddy fields was not a limiting factor, due to the availability of other wetlands capable of providing abundant food. The micro-habitat characteristics were important indicators of foraging habitat quality rather than the size of winter-flooded paddy fields, and the food accessibility may play an important role in the process of foraging habitat use. We suggest the improvement of the foraging micro-habitat and environmental characteristics would be effective in ensuring the availability of food in the dispersed lowland areas. The local people still needed to be encouraged and compensated by their single-cropping cultivation, ploughed the paddy fields after harvesting and irrigated them with shallow water flooded in the original core areas of the nature reserve.

Reduced dependence of Crested Ibis on winter-flooded rice fields: implications for their conservation.

The Crested Ibis Nipponia nippon was once thought to be extinct in the wild until seven birds were discovered in a remote mountain village in China in 1981. Studies suggested that winter-flooded rice fields play an essential role in nest site selection by the Crested Ibis and hence in their survival. Considerable efforts were therefore made to conserve the winter-flooded rice fields, but these have caused conflicts between the agricultural and conservation communities. The population and geographical range of the wild Crested Ibis has expanded greatly since 1981, but there is no spatial information on the winter-flooded rice fields, nor on the current association of nest sites and winter-flooded rice fields. We mapped winter-flooded rice fields across the entire current range of Crested Ibis using innovative remote sensing and geographical information systems (GIS) techniques. The spatial relationships between the nest site clusters and winter-flooded rice fields were quantified using Ward's hierarchical clustering method and Ripley's K-function. We show that both have significantly clumped distribution patterns and that they are positively associated. However, the dependence of Crested Ibis on the winter-flooded rice fields varied significantly among the nest site clusters and has decreased over the years, indicating the absence of winter-flooded rice fields is not constraining their recovery and population expansion. We therefore recommend that efforts should be made to protect the existing winter-flooded rice fields and to restore the functionality of natural and semi-natural wetlands, to encourage both in-situ conservation and the re-introduction of the Crested Ibis. In addition, we recommend that caution should be exercised when interpreting the habitat requirements of species with a narrow distribution, particularly when that interpretation is based only on their current habitat.

Improved molecular assay for sex identification of the endangered crested Ibis (Nipponia nippon) based on the CHD1 gene and a sex-linked microsatellite locus.

Sex Identification of monomorphic birds, especially endangered avian species, is essential for ecological study and biodiversity conservation. In this study, two popular primer sets of 2550F/2718R and P2/P8, which were designed to amplify different fragments of chromodomain-helicase-DNA binding protein 1 (CHD1) genes mapped on both Z and W chromosomes in birds, were used to identify for the first time the sex of individuals of the endangered species crested ibis (Nipponia nippon) in a large number of samples. An improved primer set of 2467F/2530R was re-designed to be specific to crested ibis following their conserved sequences derived from the 2550F/2718R primers. PCR products of the new primers were conveniently visualized with two bands of 552 base pairs (bp) and 358 bp for females, but a single band of 552 bp for males in routine 1.8% agarose gel. Similarly, the P2/P8 primer set amplified two fragments of 398 bp and 381 bp from females but one fragment of 398 bp from males; however, a high resolution involving 10% Polyacrylamide gel had to be employed to resolve the 17 bp insertions/deletions (in/dels) present between the two amplicons in females. In addition, a microsatellite locus NnNF05 was validated to be sex-linked and shown to be effective in the sexing of crested ibis, supporting its utility in non-invasive sampling. This study provides a rapid, convenient, and reliable molecular assay for improving sex identification in the monomorphic and monogamous crested ibis, and thus facilitates the selection of breeding pairs in captive programs and reintroduction initiatives.

Molecular cloning and biochemical analysis of tyrosinase from the crested ibis in China.

The crested ibis, one of the most endangered birds in the world, could benefit from research into its genetic diversity as a tool for conservation in the future. Tyrosinase is thought to play a major role in the production of common yellow to black melanins in birds. We have cloned and sequenced four exons of the crested ibis tyrosinase gene and discovered that the amino acid sequence has high similarity to zebra finch tyrosinase (93 %), followed by chicken (91 %) and quail (91 %). Some functional and structural domains in the crested ibis tyrosinase coding area were found to be conserved during evolution. Nine sequence variants were found in the partial coding sequence, one in exon 1 and eight in exon 4. Sequence variant 1 (SV1) shows intermediate polymorphism (0.25 < PIC < 0.5), and further study is needed to determine whether it can be used as a potential molecular marker in crested ibis artificial breeding programs.