RESUMEN
Electron micrography (EM) is an important method for determining the three-dimensional (3D) structure of macromolecular complexes and biological specimens. But there are several limitations such as poor signal-to-noise, limitation on range of tilt angles and sub-region subject to electron exposure, unintentional movements of the specimen, with EM systems that make the reconstruction procedure a severely ill-posed problem. A different choice of reconstruction method may lead to different results and create different additional artifacts in reconstructed images. In this paper, we combined the artifacts reduction strategy and the iterative reconstruction algorithm using a Mumford-Shah model. With the combined method, one can not only regularize the ill-posedness and provide segmentation simultaneously but also smooth additional artifacts due to the limited data. We applied the method to both simulated data from the Shepp-Logan phantom and cryo-specimen tomography. The results demonstrate the performance of the method in reducing the noise and artifacts while preserving and enhancing the edges in the reconstructed image.
Asunto(s)
Artefactos , Simulación por Computador , Tomografía con Microscopio Electrónico/métodos , Procesamiento de Imagen Asistido por Computador/métodos , Modelos Teóricos , AlgoritmosRESUMEN
Filtered back-projection and weighted back-projection have long been the methods of choice within the electron microscopy community for reconstructing the structure of macromolecular assemblies from electron tomography data. Here, we describe electron lambda-tomography, a reconstruction method that enjoys the benefits of the above mentioned methods, namely speed and ease of implementation, but also addresses some of their shortcomings. In particular, compared to these standard methods, electron lambda-tomography is less sensitive to artifacts that come from structures outside the region that is being reconstructed, and it can sharpen boundaries.