RESUMEN
Candida wangnamkhiaoensis is a species clustered under the Hyphopichia clade has not ever been isolated from any clinical specimens. To the best of our knowledge, this is the first report of C. wangnamkhiaoensis associated with fungaemia in immunocompromised paediatric patient. The isolate was assigned a strain name as UZ1679/14, in which the identification was confirmed by a polymerase chain reaction-sequencing of the internal transcribed spacer (ITS) and large subunit (LSU) regions of the rRNA gene. Antifungal susceptibility pattern showed that the isolate was sensitive to anidulafungin, caspofungin, fluconazole and voriconazole. The patient clinically improved after the antifungal treatment with caspofungin.
Asunto(s)
Candida/aislamiento & purificación , Candidemia/microbiología , Huésped Inmunocomprometido , Anidulafungina , Antifúngicos/farmacología , Candida/clasificación , Candida/genética , Candida/ultraestructura , Candidemia/tratamiento farmacológico , Candidemia/inmunología , Caspofungina , Preescolar , ADN Espaciador Ribosómico , Equinocandinas/farmacología , Equinocandinas/uso terapéutico , Fluconazol/farmacología , Humanos , Hifa/aislamiento & purificación , Hifa/ultraestructura , Lipopéptidos/farmacología , Lipopéptidos/uso terapéutico , Masculino , Filogenia , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Voriconazol/farmacologíaRESUMEN
The number of new fungal pathogens is increasing due to growing population of immunocompromised patients and advanced identification techniques. Fereydounia khargensis is a yeast and was first described in 2014 from environmental samples. As far as we know, this is the first report of human infections associated with F. khargensis. The yeasts were isolated from blood of a HIV-positive patient and pleural fluid of chronic renal failure patient. Amplification and sequencing of the internal transcribed spacer and the large subunit regions confirmed the identity of the isolates. Both isolates showed multi-drug resistance to antifungal agents tested.
Asunto(s)
Basidiomycota/efectos de los fármacos , Basidiomycota/aislamiento & purificación , Farmacorresistencia Fúngica Múltiple , Micosis/diagnóstico , Micosis/patología , Adulto , Antifúngicos/farmacología , Sangre/microbiología , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Infecciones por VIH/complicaciones , Humanos , Masculino , Técnicas Microbiológicas , Microscopía , Persona de Mediana Edad , Micosis/microbiología , Derrame Pleural/microbiología , Insuficiencia Renal/complicaciones , Análisis de Secuencia de ADNRESUMEN
Trichosporon species are opportunistic yeasts which can cause infections, especially in immunocompromised patients. This is a report of Trichosporon ovoides that caused subcutaneous infection in a patient with underlying ischemic heart disease. The identification of fungal isolate was confirmed by PCR sequencing of ITS and large subunit regions in rRNA gene. In vitro susceptibility study showed that the isolate was susceptible to amphotericin B, fluconazole and voriconazole, and resistant to caspofungin, anidulafungin and itraconazole. The lesion improved after treatment with oral fluconazole and topical miconazole.