RESUMEN
The Bachaur is a mediumized draft purpose breed which has been recognized by ICAR-National Bureau of Animal Genetic Resources (NBAGR) Karnal, India, and presently is on the verge of extinction. Since there are no data regarding the seminal parameters of this breed, this work was performed to evaluate seminal parameters of freshly ejaculated semen. A total of three healthy breeding Bachaur bulls aged 2.5-5 years were selected for the study which were maintained under identical managemental conditions. Semen parameters of these bulls were studied across 10 ejaculates. The average scrotal circumference and testicular weight of the three bulls were 27.78 ± 1.2 cm and 400.67 ± 26.6 g, respectively. The average overall volume (mL), pH, concentration (million/mL), liveability (%), abnormality (%), HOST (%) and acrosome integrity (%) were 2.20 ± 0.19, 6.86 ± 0.06, 1245.60 ± 23.49, 85.09 ± 0.91, 4.13 ± 0.06, 81.16 ± 1.18 and 83.54 ± 1.32, respectively. The average overall mass motility of three Bachaur bulls was 3.57 ± 0.06 in 0-5 scale and individual motility averaged 84.78 ± 1.70 per cent. The volume of ejaculates in Bachaur bull seemed to be lower as compared to other exotic and Indian breeds. However, the semen parameters with regard to mass motility, liveability, abnormalities, hypo-osmotic swelling test (HOST) and acrosomal integrity seemed similar to other exotic and Indian breeds.
Asunto(s)
Análisis de Semen , Semen , Motilidad Espermática , Animales , Masculino , Bovinos , Semen/fisiología , Análisis de Semen/veterinaria , India , Espermatozoides/fisiología , Testículo/anatomía & histología , AcrosomaRESUMEN
In this study, an attempt was made to evaluate the relative efficacy of two important anti-gout agents, viz. allopurinol and febuxostat, in the control of hyperuricaemia/gout using a poultry model. A 21-day study was conducted on 48 Vencobb-400 broiler chicks randomly divided into four groups. In one group hyperuricaemia/gout was induced by the oral administration of diclofenac (group D); in two other groups the ameliorative effect of the two drugs under study was investigated by providing both simultaneously, i.e. diclofenac and allopurinol (group DA), diclofenac and febuxostat (group DF); and the fourth group was kept un-induced and untreated as a control (group C). Both allopurinol and febuxostat inhibit xanthine oxidase enzymes, thereby reducing the production of uric acid. The birds kept on diclofenac alone exhibited the highest level of hyperuricaemia, clinical signs of gout, and overt adverse changes in the visceral organs, whereas these changes were lesser in allopurinol- and febuxostat-treated groups. Furthermore, haematological, biochemical, patho-morphological, and ultra-structural studies using transmission electron microscopy were carried out to evaluate the pathology and, thus, the ameliorative effect of allopurinol and febuxostat. The findings proved that allopurinol and febuxostat carry definite ameliorative potential as anti-hyperuricemic and anti-gout agents in poultry, which was better expressed by febuxostat compared to allopurinol.
Asunto(s)
Gota , Hiperuricemia , Animales , Alopurinol/farmacología , Pollos , Diclofenaco/efectos adversos , Febuxostat/farmacología , Gota/inducido químicamente , Gota/tratamiento farmacológico , Gota/veterinaria , Supresores de la Gota/farmacología , Hiperuricemia/inducido químicamente , Hiperuricemia/tratamiento farmacológico , Hiperuricemia/veterinaria , Aves de Corral , Resultado del Tratamiento , Xantina Oxidasa/farmacología , Modelos Animales de EnfermedadRESUMEN
In this investigation the morphological and morphometrical features of the optic tectum in post-hatch broiler chicken were studied macroscopically and microscopically. The present study was conducted on 70 day old broiler chicks which were reared up to 42 days. The whole experimental period of study was divided into seven groups (from group I to VII) at weekly interval (days 0, 7, 14, 21, 28, 35 and 42). The optic lobes were paired and spherical to oval eminences located on the ventro-lateral part of the midbrain in broiler chicken. There was significant increase in length and width of the optic lobes with the advancement of age. Histological analysis of optic tectum shows six basic layers from the external surface to internal one towards the optic ventricle. Different layers of optic tectum were identified as stratum opticum, stratum griseum superficial, stratum griseum central, stratum album central, stratum griseum periventriculare and stratum fibrosum periventriculare with several types of neurons. Among all six layers of the optic tectum the stratum griseum superficial layer showed very high degree of secondry differentiation and evolved into nine sub- layers in all age groups of broiler chickens. Three main cell types had been identified that is, small to medium sized stellate shaped neuron, pyramidal neuron and fusiform neuron, beside these multipolar neuron were also evident. The thickness of all layers of optic tectum significantly increases with the advancement of age of the birds. The optic ventricle was lined with a layer of cuboidal ependymal cells.
Asunto(s)
Pollos , Colículos Superiores , Animales , Colículos Superiores/anatomía & histología , Neuronas , Neuroglía , OjoRESUMEN
Rabies is a zoonotic disease caused by rabies virus of the genus Lyssa virus and family Rhabdoviridae. It affects all mammals and is prevalent throughout the world and endemic in many countries except in Islands like Australia and Antarctica. It is highly fatal, but preventable. Disease causes threat to public health because rabid dogs bite humans, resulting in thousands of deaths every year. Around 59,000 people die every year from rabies in the world. Dogs play a vital role in most of the human exposure in rabies endemic areas. Transmission of virus occurs through the bite of an infected dog. Disease is manifested by fatal nervous symptoms leading to paralysis and death. Direct fluorescent antibody technique is the gold standard for the diagnosis of the disease in animals and humans. Prevention of rabies involves the vaccination of dogs and humans before or after an exposure. This review describes the etiology, pathogenesis, diagnosis, its prevention and control strategies.
Asunto(s)
Mordeduras y Picaduras , Enfermedades de los Perros , Vacunas Antirrábicas , Virus de la Rabia , Rabia , Animales , Perros , Humanos , Rabia/diagnóstico , Rabia/epidemiología , Rabia/prevención & control , Rabia/veterinaria , Salud Pública , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/prevención & control , Zoonosis , Virus de la Rabia/fisiología , Mamíferos , Mordeduras y Picaduras/veterinariaRESUMEN
Nitric oxide (NO), a pleiotropic free radical messenger molecule, is responsible for the various cellular function of the gastrointestinal mucosa. It plays a major role in the maintenance of perfusion, regulation of microvascular, epithelial permeability, and immune functions. Nitric oxide exerts its beneficial effect on the initiation and maintenance of inflammation in human inflammatory bowel disease (IBD). But the accelerated production of NO triggers activation of the inducible form of the NO synthase enzyme (iNOS) that leads to damages of the intestinal membrane. Nitric oxide synthase enzyme is responsible for the higher production of NO from l-arginine and causes an inflammatory condition in the intestinal epithelium. Nitric oxide induces nitrative DNA damage and oxidative DNA damage in the cellular system. Accelerated production of NO enhances iNOS activity that is associated with cytotoxicity and apoptosis of gastrointestinal epithelial cells in the dog. Chronic inflammation leads to angiogenesis that is modulated by the immune system in IBD. Chronic inflammation is a major risk factor for the development of gastrointestinal malignancies. Nitric oxide participates in mucosal inflammation in the intestine through invigoration of NO synthase enzyme. The intrinsic complex mechanism is correlated with the inflammation in the gastrointestinal tract and is also correlated with the expression of iNOS, enzymatic activity and NO production. Nitric oxide employs a significant role in modulating epithelial permeability with accelerated immune response in acute colitis. But the enormous generation of NO causes adverse effects on the mucosal cell during the inflammatory process in IBD. In this review, a complex episode of NO generation with altered biochemical pathways was assessed for the regulation of mucosal inflammation in inflammatory bowel disease of dogs. This review is a unique compilation of the role of NO in the pathogenesis of inflammatory bowel disease of dogs. Nitric oxide plays a key role in modulating cancer in the gastrointestinal tract. This review seeks to explore the characteristics of NO as a major hallmark of canine inflammatory bowel diseases.
Inflammatory bowel disease (IBD), the most significant chronic inflammatory condition, is characterized by the painful infection in the gastrointestinal tract among dogs. Nitric oxide (NO) plays a significant role in counteracting the inflammation in the mucosa of the intestine. But prolonged chronic inflammation in the submucosal layers leads to accelerated production of NO that causes harmful effects on the cellular system of the intestine of IBD cases. In IBD, a complex mechanism has occurred in the intestine of dogs.
Asunto(s)
Enfermedades Inflamatorias del Intestino , Óxido Nítrico , Animales , Perros , Inflamación/patología , Inflamación/veterinaria , Enfermedades Inflamatorias del Intestino/patología , Enfermedades Inflamatorias del Intestino/veterinaria , Mucosa Intestinal/patología , Óxido Nítrico Sintasa/genética , Óxido Nítrico Sintasa/metabolismo , Óxido Nítrico Sintasa de Tipo IIRESUMEN
Nanoparticles have been attracted attention in poultry research due to their low toxicity, higher bio-availability, high surface area with sustained drug release. Dietary supplementation with selenium nanoparticles (Se-NPs) plays a regulatory role in maintaining growth performance, feed conversion ratio (FCR), antioxidant defense as well as microbial control. Se-NPs have emerging importance in modulating intestinal health through the maintenance of beneficial microbes (microflora) as well as the production of short-chain fatty acids (SCFA). Se-NPs regulate intrinsic redox status by scavenging free radicals. The antioxidant potentiality of Se-NPs is influenced by the activation of the seleno-enzymes such as thioredoxin reductase and glutathione peroxidase family (GPx) involved in scavenging of Reactive Oxygen Species (ROS). The emerging significance of Se-NPs on antimicrobial activity has been exploited due to their bio-accumulative effects and biocompatibility potentiality in the cellular systems against poultry pathogens. The present review highlights on growth performance, antioxidant defense, and anti-bacterial potentiality of Se-NPs in poultry and also provide insight into its significance in the poultry industry.
Asunto(s)
Nanopartículas , Selenio , Alimentación Animal/análisis , Animales , Antioxidantes , Aves de Corral , Selenio/farmacologíaRESUMEN
Nanotechnology, an emerging and promising technology, has been implicated to revolutionize the poultry industry. The main aspect of nanotechnology was to modify or alter the particle size into nanometers and thereby alter the physical as well as chemical features of the particular molecules. Selenium (Se), an essential trace element, can play an immense role in the maintenance of diverse physiological functions, body metabolism and cellular homeostasis, and the performance of poultry. Selenium nanoparticles (Se-NPs) are of growing importance due to its nutrients digestibility, medicinal therapy, targeted drug delivery system, and production of vaccines. Se-nanoparticles are having importance due to its high bioavailability and digestive efficiency. Se-NPs have been implicated to increase relative weights of immune-related organs (burse and thymus) to enhance immunity and thereby modulate egg production as well as the reproductive performance of birds. The present review is highlighted on the significant role of nano-selenium on reproductive performance and immunocompetence in poultry as comparative advantages over conventional sources of Se in poultry diets.
Asunto(s)
Selenio , Alimentación Animal/análisis , Animales , Inmunocompetencia , Aves de Corral , ReproducciónRESUMEN
Inflammatory bowel disease (IBD) is an important chronic condition associated with the infection affecting the gastrointestinal tract (G.I.) in dogs. Several factors' viz. gastrointestinal tract lymphoid tissue (GALT), permeability defects, genetic, ischemic, biochemical, psychosomatic disorders, infectious and parasitic agents, dietary allergies, and adverse drug reactions are associated with inflammatory bowel disease. The most noticeable clinical signs are vomiting, diarrhea, changes in appetite, weight loss, anorexia, ascites and peripheral edema. Nitric oxide (NO), a pleiotropic free radical messenger molecule plays an immense role in playing mucosal inflammation in the intestine through activation of NO synthase enzyme (iNOS). The complex mechanism associated with inflammation in the G.I. tract is also correlated with the expression of iNOS, enzymatic activity, and NO production. NO exerts a beneficial role in maintaining epithelial permeability as well as the immune response in acute colitis. But the excessive production of NO causes adverse effects. In the review, the author suggests that a complex phenomenon is associated with competing biochemical pathways triggered by NO through the regulation of mucosal inflammation in inflammatory bowel disease. This review is a unique compilation about the role of NO in the pathogenesis of inflammatory bowel disease of the dogs.
Asunto(s)
Tracto Gastrointestinal/inmunología , Tracto Gastrointestinal/metabolismo , Enfermedades Inflamatorias del Intestino/veterinaria , Óxido Nítrico/inmunología , Óxido Nítrico/metabolismo , Animales , Biomarcadores , Perros , Radicales Libres , Predisposición Genética a la Enfermedad , Humanos , Inmunidad , Enfermedades Inflamatorias del Intestino/inmunología , Enfermedades Inflamatorias del Intestino/fisiopatología , Mucosa Intestinal/fisiopatología , Óxido Nítrico Sintasa/metabolismo , Transducción de SeñalRESUMEN
Chronic arsenicosis is a major environmental health hazard throughout the world, including India. Animals and human beings are affected due to drinking of arsenic contaminated ground water, due to natural mineral deposits, arsenical pesticides or improperly disposed arsenical chemicals. Arsenic causes cancer with production of free radicals and reactive oxygen species (ROS) that are neutralized by an elaborate antioxidant defense system consisting of enzymes and numerous non-enzymatic antioxidants. Dietary antioxidant supplements are useful to counteract the carcinogenesis effects of arsenic. Oyster mushroom lectins can be regarded as ingredients of popular foods with biopharmaceutical properties. A variety of compounds have been isolated from mushrooms, which include polysaccharides and polysaccharopeptides with immune-enhancing effects. Lectins are beneficial in reducing arsenic toxicity due to anticarcinogenetic roles and may have therapeutic application in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.
Asunto(s)
Agaricales/química , Intoxicación por Arsénico/complicaciones , Carcinogénesis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Lectinas/uso terapéutico , Estrés Oxidativo/efectos de los fármacos , Animales , Intoxicación por Arsénico/fisiopatología , Carcinogénesis/patología , HumanosRESUMEN
Arsenic is one of the most hazardous substances in the environment known to cause toxicity in multiple organs. Cell adhesion, morphological alterations, cell proliferation, terminal deoxyuridine triphosphate nick-end labeling (TUNEL) and caspase-3/CPP32 fluorometric protease assay were important biomarkers to assess apoptosis in cells. This study aimed to evaluate arsenic-induced apoptosis in the hepatocytes of rat and its protective efficacy with coadministration of ascorbic acid (AA) and Pleurotus florida lectin (PFL) individually. Results of the present study also showed that arsenic caused cytotoxicity by elevating morphological alterations, TUNEL-positive nuclei, caspase-3 activity and DNA damage and reducing cell adhesion and cell proliferation in a time-dependent manner. The apoptosis in hepatocytes was reverted to normal value after coadministration of mushroom lectin in arsenic-exposed rat. The study provided significant evidence that PFL has antiapoptotic property against arsenic-induced toxicity. The beneficial effect of PFL was proportional to its duration of exposure. Retard activities of superoxide dismutase and catalase, enhanced lipid peroxidation as well as protein carbonyl in erythrocytes caused by arsenic could also be maintained toward normalcy by supplementation of AA and PFL. These antioxidative effects were exhibited in a time-dependant manner. In rat, treatment with AA and PFL prevented alteration of plasma enzyme activities caused by arsenic. The results concluded that treatment with PFL has significant role in protecting animals from arsenic-induced erythrocytic damage. This finding might be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem especially relevant to millions of people on the Indian subcontinent.
Asunto(s)
Arsénico/toxicidad , Eritrocitos/efectos de los fármacos , Lectinas/farmacología , Hígado/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Pleurotus/química , Animales , Apoptosis/efectos de los fármacos , Arsénico/sangre , Adhesión Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Hepatocitos , Etiquetado Corte-Fin in Situ , Lectinas/química , Masculino , Oxidorreductasas/metabolismo , Fagocitosis/efectos de los fármacos , Ratas , Ratas WistarRESUMEN
Arsenicosis caused due to drinking of arsenic contaminated ground water is a major environmental health hazard throughout the world. We evaluated the ecotoxicological effect of arsenic on chicken and duck in an arsenic endemic zone. The concentration of arsenic was higher in chicken and duck feed and their by-products than that in the respective samples of control area. Arsenic concentration in the eggs of both chicken and duck was higher than that in the respective samples of control area. Thus, we concluded that arsenic enters into food chain through the intake of contaminated eggs. Furthermore, adverse health effect of arsenic on avian population is due to the alteration in haematobiochemical indices.
Asunto(s)
Intoxicación por Arsénico/veterinaria , Enfermedades de las Aves de Corral/inducido químicamente , Alanina Transaminasa/sangre , Animales , Arsénico/análisis , Intoxicación por Arsénico/sangre , Intoxicación por Arsénico/epidemiología , Aspartato Aminotransferasas/sangre , Pollos/metabolismo , Patos/metabolismo , Huevos/análisis , Exposición a Riesgos Ambientales/efectos adversos , Exposición a Riesgos Ambientales/estadística & datos numéricos , Plumas/química , Hematócrito/veterinaria , Hemoglobinas/análisis , India/epidemiología , Enfermedades de las Aves de Corral/sangre , Enfermedades de las Aves de Corral/epidemiologíaRESUMEN
Arsenic is ubiquitously found metalloid that commonly contaminates drinking water and agricultural food. To minimise the ecotoxicological effect of arsenic in the environment, it is important to ameliorate the deleterious effects on human and animal health. We investigated the effects of arsenic on cattle by estimating arsenic concentration in biological samples of cattle that consumed contaminated drinking water and feedstuffs directly or indirectly. We have selected arsenic prone village that is Ghentugachi, Nadia district, West Bengal, India, along with arsenic safe control village, Akna in Hoogli district, West Bengal, India. It is found that arsenic is deposited highly in blood, urine and faeces. Agricultural field is contaminated through cattle urine, hair, faeces, cow dung cakes and farmyard manure. Bioconcentration factor and biotransfer factor are two important biomarkers to assess the subclinical toxicity in cattle, as they do not exhibit clinical manifestation like human beings.
Asunto(s)
Intoxicación por Arsénico/veterinaria , Agua Potable/química , Exposición a Riesgos Ambientales/análisis , Contaminantes Químicos del Agua/análisis , Alimentación Animal/análisis , Animales , Arsénico/análisis , Arsénico/sangre , Arsénico/orina , Bovinos , Heces/química , PoaceaeRESUMEN
The present study was undertaken to investigate the protective effect of Pleurotus florida lectin (PFL) against arsenic-induced cytotoxicity and oxidative damages in freshly isolated splenocytes of rodents. Our finding indicated that arsenic caused reduction in cell adhesion, morphological alterations, cell proliferation, nitro blue tetrazolium (NBT) index, superoxide dismutase (SOD) activity, catalase (CAT) activity and relative mRNA expression of SOD2 in relation to housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and increased production of nitric oxide (NO), protein carbonyl (PC) and lipid peroxidation levels (LPO) assembled to play key factors for cytotoxicity and oxidative stress. PFL normalized cellular damages and enhanced SOD production pathway relating to gene expression. Further studies are needed to address effective phytochemicals of the edible mushroom and their mechanism.
Asunto(s)
Productos Biológicos/farmacología , Proteínas Fúngicas/farmacología , Lectinas/farmacología , Estrés Oxidativo/efectos de los fármacos , Pleurotus/química , Sustancias Protectoras/farmacología , Animales , Arsénico/toxicidad , Adhesión Celular/efectos de los fármacos , Forma de la Célula/efectos de los fármacos , Células Cultivadas , Fragmentación del ADN/efectos de los fármacos , Peroxidación de Lípido/efectos de los fármacos , Masculino , Óxido Nítrico/análisis , Óxido Nítrico/metabolismo , Oxidorreductasas/análisis , Oxidorreductasas/metabolismo , Ratas , Ratas Wistar , Bazo/citologíaRESUMEN
Chronic arsenic exposure results in toxicity in humans and causes many toxicologic manifestations. Apoptosis was measured by cell adhesion, morphologic alterations, cell proliferation, terminal deoxyuridine triphosphate nick-end labeling (TUNEL), and caspase-3/CPP32 fluorometric protease assay. Results of the present study suggested that arsenic administration in rats caused apoptosis by elevating morphologic alterations, TUNEL-positive nuclei, caspase-3 activity, and DNA damage and by reducing cell adhesion and cell proliferation in a time-dependent manner. The apoptosis in renal cells of arsenic-exposed rats reverted to normal values after coadministration of mushroom lectin. This study provided significant evidence that Pleurotus florida lectin has an antiapoptotic property by protecting from arsenic-induced toxicity. The beneficial effect of Pleurotus florida lectin was proportional to its duration of exposure. This finding might be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.
Asunto(s)
Apoptosis/efectos de los fármacos , Intoxicación por Arsénico/prevención & control , Arsénico/toxicidad , Riñón/efectos de los fármacos , Lectinas/farmacología , Lectinas/uso terapéutico , Pleurotus , Animales , Arsénico/farmacología , Caspasa 3/metabolismo , Adhesión Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Enfermedad Crónica , Daño del ADN/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Riñón/metabolismo , Riñón/patología , Masculino , Modelos Animales , Ratas , Ratas WistarRESUMEN
The present study was planned to investigate the effect of arsenic in rats on several biochemical indices of oxidative stress. Rats were exposed to arsenite in drinking water for upto 12 weeks. Chronic exposure to arsenic for a period of 12 weeks significantly (p < 0.05) increased arsenic burden in blood, liver, and kidney. Several intrinsic antioxidant defenses were activated after a 4-week exposure to arsenic. Some remained elevated, but others became depressed over a longer exposure period. Alterations in most of the biochemical variables reached statistical significant (p < 0.05). Arsenic significantly (p < 0.01) reduced mRNA expression of the superoxide dismutase 2 (SOD2) gene with respect to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene. These observations indicated that prolong exposure to arsenic causes induction of oxidative stress and biochemical alterations.
Asunto(s)
Arsénico/toxicidad , Eritrocitos/metabolismo , Riñón/metabolismo , Hígado/metabolismo , Estrés Oxidativo/efectos de los fármacos , Animales , Arsénico/farmacocinética , Peso Corporal/efectos de los fármacos , Catalasa/metabolismo , Eritrocitos/efectos de los fármacos , Eritrocitos/enzimología , Expresión Génica/efectos de los fármacos , Riñón/efectos de los fármacos , Riñón/enzimología , Hígado/efectos de los fármacos , Hígado/enzimología , Masculino , Óxido Nítrico/metabolismo , Fagocitosis/efectos de los fármacos , Carbonilación Proteica/efectos de los fármacos , Ratas , Ratas Wistar , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismoRESUMEN
Thirty goats were selected randomly from a village of Nadia district, West Bengal according to the previous reports of human being suffering from chronic arsenicosis. Environmental samples viz. drinking water, rice plants and grass used for goat and biological samples viz. blood, urine, faeces, hair and meat were collected to evaluate the arsenic status. It was found that arsenic concentration in both environmental and biological samples was significantly (p<0.01) higher rather than respective samples on control zone. Bio-concentration factor (BCF) and bio-transfer factor (BTF) are indicated to evaluate the subclinical toxicity in goat as they do not exhibit clinical manifestation like human beings.
Asunto(s)
Intoxicación por Arsénico/veterinaria , Arsénico/análisis , Contaminantes Ambientales/análisis , Enfermedades de las Cabras/inducido químicamente , Animales , Arsénico/sangre , Arsénico/orina , Intoxicación por Arsénico/etiología , Intoxicación por Arsénico/metabolismo , Carga Corporal (Radioterapia) , Agua Potable/química , Monitoreo del Ambiente , Contaminantes Ambientales/sangre , Contaminantes Ambientales/orina , Heces/química , Cadena Alimentaria , Contaminación de Alimentos , Enfermedades de las Cabras/metabolismo , Cabras , Cabello/química , Humanos , India , Carne/análisis , Oryza/química , Poaceae/química , Medición de RiesgoRESUMEN
Natural contamination of arsenic in ground water is a major health problem throughout the World. It is one of the most hazardous substances in the environment known to cause toxicity in multiple organs via oxidative stress. The molecular basis for arsenic toxicity involves direct or indirect damage to protein, lipid and DNA. Various studies have focused on the possible toxic effects of arsenic on membrane components and its correlation with oxidative damage. The present study was aimed to mitigation of arsenic induced hepatic oxidative stress by dietary modulation using of mushroom lectin in rats. Animals were divided into four groups; the first group was used as control. Groups 2, 3 and 4 were arsenic (20 ppm) exposed through drinking water, arsenic exposed plus oral ascorbic acid (25 mg/kg body weight) and arsenic exposed plus oral mushroom lectin (150 mg/kg body weight) respectively for a period of 12 weeks. We observed significant alterations in the antioxidant enzymes, oxidative stress intermediates and SOD(2) gene expression profile on arsenic exposure. These alterations were restored by co-administration of Pleurotus florida lectin which was as potent as standard antioxidant viz. ascorbic acid. The findings of the experiment suggested that P. florida lectin has capability of modulating arsenic mediated toxic effects and could be helpful in ameliorating them.
Asunto(s)
Antioxidantes/farmacología , Arsenitos/toxicidad , Lectinas/farmacología , Hígado/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Pleurotus/química , Compuestos de Sodio/toxicidad , Contaminantes Químicos del Agua/toxicidad , Animales , Antioxidantes/aislamiento & purificación , Arsenitos/farmacocinética , Lectinas/aislamiento & purificación , Peróxidos Lipídicos/metabolismo , Hígado/enzimología , Hígado/metabolismo , Hígado/patología , Masculino , Óxido Nítrico/metabolismo , Tamaño de los Órganos/efectos de los fármacos , Carbonilación Proteica , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Compuestos de Sodio/farmacocinética , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo , Factores de Tiempo , Transcripción Genética , Contaminantes Químicos del Agua/farmacocinéticaRESUMEN
This study was accomplished to exemplify the possible protective role of ascorbic acid and mushroom lectin against arsenic-induced cytotoxicity and impairment of superoxide dismutase (SOD) production pathway in hepatocytes of rat. Hepatocytes were isolated from rat and treated with sodium arsenite (AS), arsenic plus ascorbic acid (AS + AA) and arsenic plus mushroom lectin (AS + ML). A placebo control was also included. Arsenic treatment resulted in the depletion of cell proliferation, phagocytic activity (nitro blue tetrazolium index) and superoxide dismutase (SOD) activity, relative mRNA expression of superoxide dismutase 2 (SOD(2)) and enhanced production of nitric oxide (NO). Ascorbic acid, a standard antioxidant, could normalize cellular perturbation and SOD production pathway relating to gene expression, whereas partially purified Pleurotus florida lectin (PFL), an edible mushroom containing protein complex, maintained cellular activity and prevented stress by normalizing phagocytic (NBT index) and SOD activities vis-à-vis relative gene expression. It could further defend NO production of hepatocytes. Mushroom lectin strongly prevented sodium arsenite-induced damage of SOD production pathway in hepatocytes, and its effect was also comparable to a standard antioxidant, i.e. ascorbic acid.
Asunto(s)
Arsenitos/toxicidad , Inhibidores Enzimáticos/toxicidad , Hepatocitos/efectos de los fármacos , Lectinas de Plantas/farmacología , Pleurotus/química , Compuestos de Sodio/toxicidad , Superóxido Dismutasa/antagonistas & inhibidores , Animales , Antioxidantes/farmacología , Arsenitos/antagonistas & inhibidores , Ácido Ascórbico/farmacología , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Antagonismo de Drogas , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Hepatocitos/enzimología , Masculino , Fagocitosis/efectos de los fármacos , Extractos Vegetales/farmacología , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Compuestos de Sodio/antagonistas & inhibidores , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismoRESUMEN
Oyster mushroom, Pleurotus florida is regarded as one of the popular food with biopharmaceutical properties. Here, the study aimed to investigate the antioxidative effects of mushroom (Pleurotus florida) lectin against arsenic-induced nephrotoxicity in rats. Animals were divided into four groups; Group 1 was control. Groups 2, 3 and 4 were exposed to arsenic (20 parts per million [ppm] in drinking water), arsenic plus oral supplementation of ascorbic acid (25 mg/kg body weight) and arsenic plus oral supplementation of mushroom lectin (150 mg/kg body weight) respectively. Both ascorbic acid and mushroom lectin prevented the arsenic-mediated growth retardation and normalized the elevated kidney weight. Disrupted activities of superoxide dismutase (SOD) and catalase (CAT) and enhanced lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxides (NO) production in kidney caused by arsenic could also be maintained towards normalcy by supplementation of mushroom lectin and ascorbic acid. These antioxidative effects were exhibited in a time-dependant manner. Further, arsenic-mediated down-regulation of messenger RNA (mRNA) expression of superoxide dismutase 2 (SOD(2)) gene was obstructed by these agents. Thus it was found that mushroom lectin reversed the effect of arsenic-mediated oxidative stress in a time-dependent manner.
Asunto(s)
Antioxidantes/uso terapéutico , Intoxicación por Arsénico/tratamiento farmacológico , Riñón/efectos de los fármacos , Lectinas/uso terapéutico , Pleurotus/química , Animales , Antioxidantes/aislamiento & purificación , Intoxicación por Arsénico/enzimología , Intoxicación por Arsénico/metabolismo , Intoxicación por Arsénico/patología , Peso Corporal/efectos de los fármacos , Catalasa/metabolismo , Regulación hacia Abajo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Riñón/enzimología , Riñón/metabolismo , Riñón/patología , Lectinas/aislamiento & purificación , Peroxidación de Lípido/efectos de los fármacos , Masculino , Óxido Nítrico/metabolismo , Tamaño de los Órganos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Carbonilación Proteica , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo , Factores de TiempoRESUMEN
Acute and chronic arsenic exposure result in toxicity both in human and animal beings and cause many hepatic and renal manifestations. The present study stated that mushroom lectin prevents arsenic-induced apoptosis. Apoptosis was measured by morphological alterations, cell proliferation index (CPI), phagocytic activity (nitro blue tetrazolium index; NBT), nitric oxide (NO) production, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, DNA fragmentation and caspase-3 activity. Arsenic exposure at 5 µM in the form of sodium arsenite resulted in significant elevation of deformed cells, NO production, TUNEL stained nuclei of hepatocytes, DNA fragmentation and caspase-3 activity. But the CPI and NBT index were significantly declined in arsenic-treated hepatocytes. The beneficial effect of mushroom lectin at 10 µg/mL, 20 µg/mL and 50 µg/mL) showed increased CPI and phagocytic activity. Mushroom lectin at those concentrations reduced deformed cells, NO production, DNA fragmentation and caspase-3 activity of hepatocytes. But significant better protection was observed in 50 µg/mL mushroom lectin-treated hepatocytes. This finding may be of therapeutic benefit in people suffering from chronic arsenic exposure.