Gene expression analysis of alkaline phosphatase in peri-implantitis tissue.

Peri-implantitis is a condition that causes inflammation and bone loss around dental implants. Alkaline phosphatase (ALP) is a gene involved in the inflammation of the tissue. Therefore, it is of interest to evaluate the expression of the ALP gene around the peri-implantitis site. 20 samples were collected and analyzed using qRT-PCR, and statistical analysis was performed. The results showed a significant decrease in ALP expression in infected peri-implantitis tissue compared to normal tissue, indicating that ALP gene is involved in the inflammation of peri-implantitis and correlates with clinical findings.

STITCH enabled protein-protein interactions between Phyllanthus emblica and peri-implant microbiome.

It is of interest to document the protein-protein interaction between Phyllanthus emblica and peri implant pathogens in the context of peri implant illness. The peri implant pathogens includes Aggregatibacter actinomycetemcomitans (D7S-1), Centipeda periodontii, Campylobacter gracilis, Fusobacterium nucleatum, Slackia exigua, Prevotella intermedia, Tannerella forsythia, Staphylococcus aureus, Bacteroides fragilis, and Bacteroides fragilis. Hence, a user-defined query was used to conduct analysis on the provided bacterial strains whose molecular data available in the STITCH database. Thus, we used the STITCH tool to examine protein interactions and the VirulentPred tool to assess pathogenicity using the known molecular data on Phyllanthus emblica and peri implant pathogens. Data shows that Phyllanthus emblica interacts with peri implant pathogens.

Correlation between CT - derived bone density and optimal bone densities acquired from CBCT scans.

It is of interest to explore the correlation between preoperative bone density, assessed via CBCTT, and primary stability of dental implants, assessed by torque ratchet. A total of 100 patients who had implant placed were taken a sample for this retrospective study. The Hounsfield units (HU) derived preoperative bone densities at implant sites that were acquired with the help of the CBCT and the primary stability was achieved during the day of surgery. Both were compared to optimum bone densities. Statistical correlation was done between the HU and Bone density. Data suggests that evaluating HU values, along with other parameters, before performing implant surgery could lead to better primary implant stability.

Efficacy of mucolytics and steam therapy in the management of sinusitis among Indians

It is of interest to compare the treatment modalities of sinus membrane thickening, by analyzing the difference in pre and post-intervention radiographic measurement of sinus membrane thickness. Results showed that combination therapy of steam and mucolytics decreased the sinus thickening in a statistically significant manner compared to mucolytics alone. Thus, there is a correlation between maxillary sinus membrane thickening and patency of maxillary ostium radiographically.

Influence of antagonist tooth on mandibular implant positioning during surgery among Indians

A retrospective radiographic analysis of cone beam computed tomographic radiographs of 42 patients who had undergone dental implant therapy at the department of implantology, Saveetha Dental College Hospitals, India. The mean angular deviation was 3.17 ° in the anterior, 1.6° in the premolar and 0.81° in the molar region. Data shows that free hand placement could be done with minimal deviation taking the opposing dentition as a guide.

Effectiveness of Herbal, Homeopathic and Conventional Dentifrices on Dental Caries - A Double-Blind Randomised Controlled Trial.

PURPOSE: To compare the effectiveness of ayurvedic, homeopathic and conventional dentifrices on plaque and saliva in terms of cariogenic bacteria, salivary pH, and plaque pH. MATERIALS AND METHODS: This double-blinded, parallel-group, randomised controlled trial was performed at Saveetha Dental College and Hospitals, Chennai, India. The participants comprised healthy adults possessing more than 20 permanent natural teeth and having a Decayed Missing and Filled Teeth (DMFT) score, plaque index score, and gingival index score less than or equal to 2. There were 3 intervention groups: 1: herbal dentifrice (Dabur Meswak); 2: homeopathic dentifrice (Gum Forte gel); 3: fluoride dentifrice (Colgate Total). The outcome measures were as follows: plaque and saliva samples were evaluated for pH; colony counts of Streptococcus mutans and Lactobacillus at baseline, 14 and 28 days of follow-up. One-way and repeated measures ANOVA, Wilcoxon signed-rank and Kruskal Wallis tests were used to compare the mean differences of plaque and salivary pH and plaque and salivary S. mutans and Lactobacillus counts at baseline, 14 and 28 days. RESULTS: The mean S. mutans and Lactobacillus counts in plaque and saliva decreased statistically significantly in all treatment groups at the 28-day follow-up. Mean plaque pH was not statistically significantly different at the 14-day follow-up (p-value = 0.16). On the 28th day, group 1 (7.64 ± 0.20) showed the highest increase in plaque pH followed by group 2 (7.39 ± 0.25) and group 3 (7.27 ± 0.19), which was found to be statistically significant. No statistically significant difference in mean salivary pH was observed between the three groups at the different time points. CONCLUSION: This study reveals that the herbal dentifrice tested here was effective in reducing cariogenic bacterial count and increasing the plaque pH, thereby warranting the usage of the same.

Chitosan-Reinforced Gelatin Microspheres-Modified Glass Ionomer Cement (GIC): A Novel Bone Alloplast Graft Material Synthesis and an In Vivo Analysis.

Aim and objective The study aimed to assess and evaluate the efficacy of glass ionomer modified with chitosan-reinforced gelatin microspheres on bone formation. Materials and methods  The study involved three groups: Group I comprised plain glass ionomer cement; Group II comprised glass ionomer cement/gelatin (70:30 wt%); in Group III, glass ionomer cement/gelatin/chitosan (70:30%) scaffold were made into discs; the gelatin microspheres were synthesized by oil emulsion method. The synthesized scaffold was subjected to the following in vitro testing, Instron Universal Testing Machine (UTM), U3000, (Instron Corporation, Norwood, Massachusetts, United States) to assess compressive strength, scanning electron microscope (SEM) examination, and biocompatibility testing using hemocompatibility assay. The material was then tested in vivo; male Wistar albino rats, a total of nine animals, were utilized for this purpose. Three animals were used in each group; a femoral defect model was the model of choice for the experiment and the animals were observed for a period of four weeks, following which the animals were sacrificed and sent for histopathological analysis. Results The compression testing was carried out using UTM; test group I was 33 MPa, test group II was 2.3 MPa, and test group III was 25.75 MPa. SEM (JSM-IT800 Schottky Field Emission NANO SEM (JEOL, Tokyo, Japan)) analysis was done to observe the porosity of the fabricated scaffold with the average measurement of 0.12 ± 0.2 µm in test group II and 0.29 ± 0.4 µm in test group III. Hemocompatibility reports noted 0.4-0.8% lysis for the synthesized scaffolds. Histopathology staining of the femur defects showed that group III favoured bone formation. One-way analysis of variance (ANOVA) and post hoc Bonferroni test was done on the data. The optical density values of the alizarin red stained slide showed statistical significance for group III. Conclusion In conclusion, the synthesized scaffolds are biocompatible, distribution of porosity and pore characteristics in the glass ionomer cement/gelatin/chitosan group is better than that of the glass ionomer cement/gelatin group. The glass ionomer cement/gelatin/chitosan group had better compressive strength and induced more bone formation compared to the other test group and the control. Thus, the novel glass ionomer modified with chitosan-reinforced gelatin microspheres has optimal properties to be used as a bone graft material.

Workflow for the full-mouth rehabilitation of a patient with different prosthetic materials in opposing arches: A bold move in desperate scenario.

Fixed rehabilitation is an exhaustive approach for most dental practitioners to treat partial or complete edentulism. In recent years, we are witnessing a digital transformation in prosthodontics and smile makeovers. This requires an in-depth understanding of concepts and the skills to achieve desired clinical results. Many situations of the oral cavity often need fixed rehabilitation treatment. This extensive treatment primarily solves two goals: maintains crown integrity and also achieves optimal esthetics. To restore lost parts of the stomatognathic system, a multidisciplinary approach is of utmost importance. The treatment options may range from removable implant-supported dentures to fixed implant-supported prostheses. The choice of treatment relies on the limitations of anatomic structures and existing. Partial or complete edentulousness can inadvertently reduce the quality of life of the patient and may hinder physiology. This report narrates a combination of two types of dental prosthetic material to obtain a desirable outcome in fixed rehabilitation of a patient. Furthermore, it highlights the management of complete treatment in a limited period and patient appointments.