RESUMEN
The presence and molecular characterization of Clostridium perfringens in healthy Merino lambs over a six-month period was investigated in this study. Overall, a high prevalence of C. perfringens was detected, even in day-old lambs. Even though the majority of the isolates were characterized as being of type A, types C and D were also isolated. Furthermore, a high genetic diversity was observed by PFGE among the type A isolates.
Asunto(s)
Infecciones por Clostridium/veterinaria , Clostridium perfringens/genética , Enfermedades Gastrointestinales/veterinaria , Variación Genética , Enfermedades de las Ovejas/microbiología , Animales , Argentina/epidemiología , Infecciones por Clostridium/epidemiología , Infecciones por Clostridium/microbiología , Clostridium perfringens/aislamiento & purificación , Electroforesis en Gel de Campo Pulsado/veterinaria , Femenino , Enfermedades Gastrointestinales/epidemiología , Enfermedades Gastrointestinales/microbiología , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , Ovinos , Enfermedades de las Ovejas/epidemiologíaRESUMEN
In order to know whether IFN alpha prevents in vivo oncogenesis in the very-early-stage cancer cells, we evaluated the action of IFN alpha-2b on preneoplastic foci in rats. Animals were divided into six groups: subjected to an initiation-promotion model of cancer development (G1), treated with IFN alpha-2b during: a) initiation-promotion (G2), b) initiation (G3), promotion (G4); subjected only to an initiation stage (G5) and treated with IFN alpha-2b during this period (G6). The number and area of rGST P-positive foci were reduced and the Apoptotic index was increased in G2, 3 and 6. Bcl-2 and Bcl-XL protein levels were decreased in IFN alpha-2b-treated rats. Increased levels of mitochondrial Bax protein were observed in G2, 3 and 6. In conclusion, preneoplastic hepatocytes in the IFN alpha-2b-treated rats undergo programmed cell death as a result of a significant increase of Bax and its translocation to the mitochondria.