Comparison of the Idylla™ Respiratory (IFV-RSV) panel with the GeneXpert Xpert® Flu/RSV assay: a retrospective study with nasopharyngeal and midturbinate samples.

The objective of this study was to compare the performance of the Idylla™ Respiratory (IFV-RSV) panel to the GeneXpert Xpert® Flu/RSV assay and establish the performance of a midturbinate swab compared to nasopharyngeal sampling. Considering GeneXpert® assay as imperfect reference standard, a positive percentage agreement between both assays of 98-100% for influenza A and 96-99% for influenza B could be calculated when 354 nasopharyngeal and 325 midturbinate swabs were retrospectively analyzed. Comparing midturbinate samples to nasopharyngeal specimens of 321 subjects, positive percentage agreement varied from 42% to 94% depending on both target virus and assay used. Negative percentage agreements ranged from 98% to 100% for both methods and sample type comparison. The Idylla™ assay showed excellent performance compared to the GeneXpert® assay for the detection of influenza virus. The study also showed a slightly better performance for nasopharyngeal sampling compared to the use of a midturbinate swab.

Comparison between J-aggregates in a self-assembled multilayer and polymer-bound J-aggregates in solution: a steady-state and time-resolved spectroscopic study.

The self-assembly of a thiacarbocyanine dye on a charged substrate via the layer-by-layer alternate adsorption technique was studied in detail. The influence of the chemical structure of the oppositely charged polyelectrolyte (PEL) in the multilayer on J-aggregate formation was investigated by means of absorption spectroscopy and steady-state and time-resolved fluorescence spectroscopy. J-Aggregates were formed yielding a bathochromic narrow absorption band. Fine tuning of the position of the absorption band is possible by incorporating other polyelectrolytes in the multilayer. The results obtained for the dye-polyelectrolyte multilayers were compared with measurements made on the aqueous dye-polyelectrolyte mixtures. The parameters obtained by fitting the fluorescence decays to several different models allowed us to suggest a mechanism for the non-radiative decay channel. The low fluorescence quantum yields and fast non-exponential fluorescence decays obtained for both the dye-polyelectrolyte multilayers and the aqueous dye polyelectrolyte mixtures were attributed to the trapping of a fast migrating exciton by a non-luminescent trap. The results related to J-aggregate formation, decay time and dimensionality of the aggregates were compared in solution and in a self-assembled multilayer.