RESUMEN
PURPOSE: Unpredictable genetic modifications and chromosomal aberrations following CRISPR/Cas9 administration hamper the efficacy of germline editing. Repair events triggered by double-strand DNA breaks (DSBs) besides non-homologous end joining and repair template-driven homology-directed repair have been insufficiently investigated in mouse. In this work, we are the first to investigate the precise repair mechanisms triggered by parental-specific DSB induction in mouse for paternal mutational correction in the context of an infertility-related mutation. METHODS: We aimed to correct a paternal 22-nucleotide deletion in Plcz1, associated with lack of fertilisation in vitro, by administrating CRISPR/Cas9 components during intracytoplasmic injection of Plcz1-null sperm in wild-type oocytes combined with assisted oocyte activation. Through targeted next-generation sequencing, 77 injected embryos and 26 blastomeres from seven injected embryos were investigated. In addition, low-pass whole genome sequencing was successfully performed on 17 injected embryo samples. RESULTS: Repair mechanisms induced by two different CRISPR/Cas9 guide RNA (gRNA) designs were investigated. In 13.73% (7/51; gRNA 1) and 19.05% (4/21; gRNA 2) of the targeted embryos, only the wild-type allele was observed, of which the majority (85.71%; 6/7) showed integrity of the targeted chromosome. Remarkably, for both designs, only in one of these embryos (1/7; gRNA 1 and 1/4; gRNA2) could repair template use be detected. This suggests that alternative repair events have occurred. Next, various genetic events within the same embryo were detected after single-cell analysis of four embryos. CONCLUSION: Our results suggest the occurrence of mosaicism and complex repair events after CRISPR/Cas9 DSB induction where chromosomal integrity is predominantly contained.
Asunto(s)
Sistemas CRISPR-Cas , Roturas del ADN de Doble Cadena , Edición Génica , Animales , Sistemas CRISPR-Cas/genética , Ratones , Femenino , Edición Génica/métodos , Masculino , Oocitos/crecimiento & desarrollo , Oocitos/metabolismo , Infertilidad/genética , Infertilidad/terapia , Mutación/genética , Reparación del ADN/genética , Embrión de Mamíferos , Inyecciones de Esperma Intracitoplasmáticas/métodos , ARN Guía de Sistemas CRISPR-Cas/genética , Reparación del ADN por Unión de Extremidades/genéticaRESUMEN
The chromosome complement of human spermatozoa has been analyzed after their intracytoplasmic injection into unfertilized mouse oocytes. A total of 427 metaphase plates have been obtained, including 176 metaphase plates from spermatozoa with normal head morphology (108 and 68 spermatozoa from patients with normal (the control group) and abnormal spermogram parameters, respectively), and 251 metaphase plates from spermatozoa with abnormal heads (76, 91, 67, and 17 spermatozoa with large, amorphous, elongated, and round heads, respectively). The frequency of chromosome abnormalities in the control group is 26.1%, with hyperploidy, hypoploidy, and structural aberrations accounting for 7.4, 12.3, and 6.4% of the abnormalities, respectively. In none of the groups did the ratio between the numbers of X- and Y-bearing spermatozoa significantly differ from 1 : 1. The diploidy frequency was significantly higher in spermatozoa with large and amorphous heads compared to the control group (2.36, 3.29, and 0%, respectively). None of the groups of spermatozoa differed from the control group with respect to the frequency of structural aberrations. The type of the abnormal head morphology has been found to be correlated with the sperm chromosome complement.
Asunto(s)
Aberraciones Cromosómicas , Infertilidad Masculina/genética , Oocitos , Cabeza del Espermatozoide , Inyecciones de Esperma Intracitoplasmáticas , Animales , Análisis Citogenético , Femenino , Humanos , Cariotipificación , Masculino , Ratones , Cabeza del Espermatozoide/patologíaRESUMEN
The fact that most of the advances in mouse cloning by nuclear transfer originate from research in a limited number of laboratories demonstrates the complexity of the reported technologies. The development of alternative and more simple techniques of nuclear transfer may therefore be of interest. Furthermore, the preimplantation biology of cloned mouse embryos originating from somatic cells has not yet been studied in detail. In the present study, a modified conventional injection (mCI) technique for cloning mice from somatic cells is described. The preimplantation development and morphology of the resulting nuclear transfer embryos in comparison with parthenogenetic embryos and embryos obtained by intracytoplasmic sperm injection (ICSI) under comparable conditions was also studied. Finally, the capacity of nuclear transfer embryos for full-term development was investigated. Eighty-nine per cent of oocytes injected with cumulus cell nuclei under mCI conditions survived and formed zygotes. However, the rate of development of these zygotes to the blastocyst stage was significantly lower (29%) than that of ICSI or parthenogenetic zygotes (95 and 92%, respectively). Cloned blastocysts had a significantly lower mean number of cells in the inner cell mass (9) and trophectoderm (52) and a lower inner cell mass:total cell ratio (14%) than did their counterparts (31, 143 and 18% for ICSI and 21, 92 and 18% for parthenogenetic blastocysts, respectively). This correlated with a significantly higher proportion of dead cells in the cloned blastocysts. The poor quality of cloned blastocysts may explain the low rate of full-term fetal development of somatic mouse clones.
Asunto(s)
Clonación de Organismos/métodos , Desarrollo Embrionario y Fetal , Técnicas de Transferencia Nuclear , Animales , Blastocisto/citología , Frío , Transferencia de Embrión , Femenino , Sangre Fetal , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos DBA , Microinyecciones , Partenogénesis , Inyecciones de Esperma IntracitoplasmáticasRESUMEN
The effect of simple and sequential embryo culture media on the preimplantation development of mouse nuclear transfer (NT) embryos reconstructed with cumulus cell nuclei using a mechanical NT technique was studied. Blastocyst formation rate was evaluated using CZB medium and the sequential media G1/G2 and KSOM/G2. Arrested two- and three-cell NT embryos were Hoechst-stained to check for nuclear abnormalities. Nonmanipulated and sham-manipulated parthenogenetic embryos served as controls for, respectively, the medium and the handling technique. Rates of blastocyst formation for medium and handling control embryos were similar in CZB (58% and 61%), in G1/G2 (94% and 85%), and in KSOM/G2 (88% and 84%). Development of NT embryos was significantly impaired from the two-cell stage onwards, reaching the blastocyst stage at a rate of 5% in CZB, 14% in G1/G2, and 28% in KSOM/G2. Arrested two- and three-cell stage NT embryos showed a high rate of binucleation. These data demonstrate not only that NT embryos are more sensitive to in vitro culture conditions than parthenogenetic control embryos but also that selection of culture media can influence the preimplantation development of NT embryos.
Asunto(s)
Clonación de Organismos/métodos , Medios de Cultivo/farmacología , Animales , Blastocisto/fisiología , Núcleo Celular/metabolismo , Ratones , Ratones Endogámicos C57BL , Ploidias , Factores de Tiempo , Cigoto/fisiologíaRESUMEN
Obtaining karyotypes from human spermatozoa after microinjection into Syrian golden hamster oocytes is difficult and the hitherto reported results are unsatisfactory. This may be related to the injection and culture technique or to the high susceptibility of the hamster oocytes to undergo parthenogenetic activation or both. Therefore, we investigated the hamster oocyte-human sperm microinjection model using the following two approaches: (i) application of contemporary techniques for injection (touching the sperm tail) and culture (hamster embryo culture medium, HECM-3, 10% CO2) and (ii) omission of Ca2+ from the injection medium. Thus, in the first series of experiments, 252 hamster oocytes were injected with human spermatozoa. Among the 219 (87%) oocytes that survived the injection procedure, the mean percentages of male pronucleus formation [two pronuclei (2PN), two polar bodies (PB)], mitotic metaphase entry and sperm chromosome spreads were 41.4, 27.8 and 18.2% respectively. Analysis of the oocytes which failed to develop the male pronucleus following injection revealed that most of them had developed only the hamster female PN while the sperm nuclei were either intact or swollen (partially decondensed), indicating that failure of oocyte activation was not the likely reason for the failure of male PN formation in these oocytes. In the next series of experiments, sibling oocytes were alternately injected with spermatozoa suspended either in the regular (1.9 mM Ca2+) or Ca2+-free injection medium (experiment set 2, n=278). A significant improvement was noted in the mean percentages of oocytes with 2PN, 2PB, metaphase entry and sperm chromosome spreads in the Ca2+-free group versus the regular group (2PN, 2PB: 51 versus 36.6%, metaphase entry: 36.3 versus 26.9% and sperm chromosome spreads: 28 versus 20.4%; all P < 0.04). Thus, parthenogenetic activation appears to be one of the contributing factors for the failure of male PN formation after heterospecific hamster ICSI. From these experiments it can be concluded that application of the advanced injection and culture techniques and omission of Ca2+ from the injection medium are promising for the routine application of the hamster oocyte microinjection for karyotyping of human spermatozoa with poor fertilizing capacity.
Asunto(s)
Fertilización In Vitro/métodos , Oocitos/ultraestructura , Espermatozoides/ultraestructura , Animales , Calcio , Núcleo Celular/ultraestructura , Cromatina/ultraestructura , Aberraciones Cromosómicas , Cromosomas Humanos/ultraestructura , Cricetinae , Medios de Cultivo , Citoplasma/ultraestructura , Femenino , Humanos , Técnicas In Vitro , Infertilidad Masculina/genética , Infertilidad Masculina/patología , Infertilidad Masculina/terapia , Cariotipificación , Masculino , Mesocricetus , Metafase , Microinyecciones , PartenogénesisAsunto(s)
Fertilización In Vitro/métodos , Infertilidad Masculina/terapia , Oocitos/fisiología , Espermatozoides/anomalías , Animales , Fertilización In Vitro/efectos adversos , Humanos , Masculino , Ratones , Microinyecciones , Espermatozoides/química , Espermatozoides/citología , Insuficiencia del TratamientoRESUMEN
Intracytoplasmic sperm injection of immotile (dead) ejaculated human spermatozoa has been carried out by several centres for the treatment of infertility caused by severe asthenozoospermia (necrozoospermia). No healthy pregnancies have been reported as yet, suggesting irreversible damage to sperm DNA, centrioles and/or other important structures. We investigated this hypothesis by injection of immotile human spermatozoa obtained from several male infertility patients into mouse oocytes and analysis of the oocyte activation rate and sperm chromosome integrity. Motile spermatozoa of the same sample were used as a control. The proportion of living spermatozoa among the immotile was also assessed in each sample and was related to the results of the mouse oocyte injection test. The oocyte activation rate after injection of immotile human spermatozoa into mouse oocytes was the same or only slightly lower than after injection with initially motile spermatozoa (87-100% versus 100% respectively). The rate of normal sperm chromosome spreads correlated significantly (r = 0.90, P < 0.05) with the proportion of living immotile spermatozoa in a given sample. It varied from 4 to 48% for samples containing respectively 8 and 40% of living spermatozoa. Most of the mouse oocytes injected and activated with immotile human spermatozoa were arrested during a prolonged period of time at the interphase of the first cell cycle (from 22 to 60%). Others underwent a delayed nuclear envelope breakdown but showed signs of abnormal structure of both male and female or only the male pronuclear chromosomes. Our data demonstrate an irreversible damage of chromosomes in dead ejaculated human spermatozoa and provide an experimental basis for recommending the use of testicular or epididymal spermatozoa for treatment of male infertility due to necrozoospermia.
Asunto(s)
Aberraciones Cromosómicas , Daño del ADN , Fertilización In Vitro , Motilidad Espermática/fisiología , Interacciones Espermatozoide-Óvulo , Animales , Citoplasma , Femenino , Humanos , Cariotipificación , Masculino , Ratones , Microinyecciones , OocitosAsunto(s)
Fertilización In Vitro/métodos , Microinyecciones , Animales , Citoplasma , Femenino , Humanos , MasculinoRESUMEN
We have previously shown that sperm plasma membrane damage makes the sperm plasma membrane permeable and the sperm nucleus accessible for low molecular weight molecules such as eosin and dithiothreitol. In the present study, we investigated whether this damage is associated with a passive release of the sperm-associated oocyte activating factor (SAOAF) from the spermatozoon and, if so, its time sequence. In a first study, human oocytes remaining unfertilized after conventional in-vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) and freshly ovulated mouse oocytes were injected with a whole spermatozoon or a sperm head respectively. They were randomly allocated to one of three groups: oocytes in group 1 were injected with a spermatozoon immobilized or sperm head detached immediately prior to the injection; oocytes in group 2 were injected with a spermatozoon immobilized or sperm head detached 2-4 h before injection; oocytes in group 3 were injected with a spermatozoon or sperm head that had been subjected to heat treatment. The activation rate of oocytes injected with a spermatozoon or sperm head was the same for groups 1 and 2, and significantly higher than in group 3 (P < 0.001). In a second series of experiments, human oocytes remaining unfertilized after IVF or ICSI were injected with a sperm head that was subsequently removed from the ooplasm 20-30 min after injection. The activation rates were compared to that of oocytes injected with heat-treated spermatozoa which subsequently were removed from the ooplasm. We found that the removal of the spermatozoon 30 min after injection did not prevent oocyte activation. Our data indicate that the initial damage to the sperm plasma membrane induced at immobilization, although essential for the onset of sperm nuclear swelling after ICSI, does not by itself lead to the release of SAOAF from the spermatozoon. We postulate, however, that SAOAF is released during the sperm nuclear swelling phase, which is induced by the so-called sperm nucleus decondensing factor (SNDF) of the oocyte.
Asunto(s)
Fertilización In Vitro/métodos , Microinyecciones , Oocitos/fisiología , Proteínas/metabolismo , Interacciones Espermatozoide-Óvulo/fisiología , Espermatozoides/fisiología , Animales , Proteínas de Unión al Calcio , Femenino , Cabeza , Humanos , Cinética , Masculino , Ratones , Oocitos/efectos de los fármacos , Proteínas/farmacología , Cabeza del EspermatozoideRESUMEN
OBJECTIVE: To investigate a method of assisted activation of human oocytes for the treatment of infertility resulting from globozoospermia associated with deficient oocyte activation capacity. DESIGN: The mouse oocyte activation test was used to analyze the oocyte activation capacity of the sperm cells of a patient with globozoospermia. Fresh donor human oocytes were used for determining the most appropriate procedure for oocyte activation. SETTING: Infertility Center, University Hospital of Ghent. PATIENT(S): A couple with infertility resulting from globozoospermia. INTERVENTION(S): Intracytoplasmic sperm injection, assisted oocyte activation, and embryo transfer. MAIN OUTCOME MEASURE(S): Oocyte activation and fertilization rates, implantation, and pregnancy. RESULT(S): Deficiency in oocyte activation capacity was found in the sperm of a patient with globozoospermia. This deficiency was proven by the mouse oocyte activation test and was confirmed further by lack of activation of human oocytes after simple sperm injection. Only human oocytes that were injected with sperm and subjected to calcium chloride and ionophore treatment underwent activation. Transfer of embryos obtained by this procedure of assisted oocyte activation resulted in an ongoing pregnancy. CONCLUSION(S): Assisted oocyte activation of human oocytes is useful when globozoospermia is associated with absence of oocyte activation capacity in the sperm. These cases can be identified by the mouse oocyte activation test.
Asunto(s)
Calcimicina/farmacología , Fertilización In Vitro/métodos , Infertilidad Masculina/patología , Infertilidad Masculina/terapia , Ionóforos/farmacología , Oocitos/efectos de los fármacos , Oocitos/fisiología , Espermatozoides/anomalías , Adulto , Animales , Citoplasma , Femenino , Humanos , Masculino , Ratones , Microinyecciones , Donación de Oocito , EmbarazoRESUMEN
The influence of unprotected freezing of mammalian spermatozoa on their oocyte activating capacity and chromosome integrity is unknown. However, this type of sperm treatment has been used in assisted reproduction by intracytoplasmic sperm injection in cattle and humans. The mouse oocyte injection test was used to analyse the influence of unprotected freezing of human spermatozoa on their reproductive characteristics. Mouse oocytes were microinjected with intact human spermatozoa or spermatozoa treated with two cycles of unprotected freeze-thawing. Oocytes surviving the injection were either cultured without further treatment or exposed to ethanol solution to induce parthenogenetic activation. Both injected and activated oocytes were used for sperm chromosome analysis. The results revealed a significant reduction in oocyte activating capacity and a tenfold increase in the incidence of structural chromosomal abnormalities in human spermatozoa treated by unprotected freezing. We conclude that unprotected freezing of human spermatozoa has a detrimental effect on their reproductive characteristics. Our data also provide a new perspective on the stability of mammalian spermatozoa to physical factors and demonstrate the importance of detailed analysis of the stability of sperm structures for successful development of new approaches in assisted reproduction.
Asunto(s)
Cromosomas Humanos , Criopreservación/métodos , Oocitos/fisiología , Capacitación Espermática , Espermatozoides/fisiología , Animales , Membrana Celular/patología , Aberraciones Cromosómicas , Bandeo Cromosómico/métodos , Eosina Amarillenta-(YS)/química , Femenino , Fertilización In Vitro , Congelación , Técnicas Genéticas , Humanos , Masculino , Ratones , Ratones Endogámicos , Interacciones Espermatozoide-Óvulo , Espermatozoides/ultraestructuraRESUMEN
Intracytoplasmic sperm injection (ICSI) in the human is a very effective procedure which allows the fertilization of the majority of oocytes even in cases of extreme oligoasthenoteratozoospermia. Round-headed acrosomeless human spermatozoa, however, form an exception to this rule, because in about half of the couples with globozoospermia all oocytes remain unfertilized after injection. The incapacity of the spermatozoon to activate the oocyte following injection of round-headed spermatozoa could be the underlying mechanism. To investigate this hypothesis, activation rates of mouse oocytes injected with spermatozoa from a patient with globozoospermia were compared with those obtained after injection with normal spermatozoa. Of mouse oocytes surviving the injection with donor spermatozoa, 95% underwent activation, compared to none of the 88 mouse oocytes surviving the injection with round-headed spermatozoa. After fixation, prematurely condensed sperm chromosomes were found in these oocytes. Parthenogenetic activation of mouse oocytes (8% ethanol at 40 min after injection) injected with round-headed spermatozoa led to the activation of 96% of oocytes. These oocytes developed normally to the first mitosis and were fixed for analysis of the sperm karyotypes. The incidence of chromosomal abnormalities of round-headed spermatozoa (6%) was similar to that in spermatozoa from a fertile donor (9%). These data provide further information on the basic defect in cases of globozoospermia and demonstrate that globozoospermia is not associated with sperm karyotype abnormalities.
Asunto(s)
Cromosomas , Oocitos/fisiología , Cabeza del Espermatozoide/ultraestructura , Interacciones Espermatozoide-Óvulo , Espermatozoides/fisiología , Espermatozoides/ultraestructura , Adulto , Animales , Centrómero/ultraestructura , Aberraciones Cromosómicas , Trastornos de los Cromosomas , Etanol/farmacología , Femenino , Humanos , Cariotipificación , Masculino , Ratones , Microinyecciones , Oocitos/efectos de los fármacosRESUMEN
Winston et al. ([1995] J. Cell Sci., 108:143-151) have shown recently that short (6 min) exposure of spindle intact oocytes from Swiss mice to 8% ethanol induced activation of most oocytes, while disruption of the spindles in these oocytes by nocodazole, before and during ethanol exposure, completely inhibited oocyte activation. We compared the activation rates (ARs) of nocodazole-treated and intact oocytes recovered from SJL and B6D2 F1 hybrid mice under the same experimental conditions. The difference between the ARs of nocodazole-treated and intact SLJ oocytes was about the same as reported for Swiss oocytes (2% vs. 82%, respectively). In contrast, this difference was minor for B6D2 oocytes (87% vs. 100%, respectively). Moreover, 41% of these oocytes underwent activation when the spindle was absent, not only before and during, but also 2 h after ethanol exposure. Shortened exposure (2 min) of B6D2 oocytes to ethanol, however, increased the difference in the ARs of nocodazole-treated and intact oocytes (18% vs. 67%, respectively). We conclude that at least two parameters affect the necessity of the presence of the spindle during ethanol exposure for the activation of mouse oocytes. They are the genotype of the oocytes and the duration of exposure to ethanol. Under one set of these parameters the presence of the spindle is absolutely necessary, while under the other the appearance of the spindle a few hours after ethanol exposure is sufficient to allow the activation of some oocytes.
Asunto(s)
Etanol/farmacología , Oocitos/efectos de los fármacos , Huso Acromático/efectos de los fármacos , Animales , Femenino , Genotipo , Técnicas In Vitro , Ratones , Ratones Endogámicos , Nocodazol/farmacología , Oocitos/citología , Oocitos/fisiología , Huso Acromático/fisiología , Factores de TiempoRESUMEN
In the present study we investigated the relevance of sperm immobilization prior to intracytoplasmic sperm injection (ICSI) in the fertilization process. Using supravital staining of the spermatozoa with eosin and studying sperm decondensation with 2 mM dithiothreitol (DTT) in conditions imitating sperm handling during ICSI, we demonstrated that immobilization of the spermatozoon by squeezing its tail between the glass pipette and the bottom of the dish damages the sperm plasma membrane. Polyvinylpyrrolidone (PVP), which is usually present in the drop with the spermatozoon to facilitate its handling, was found to impede the access of both eosin and DTT to the sperm nucleus. We conclude that (i) sperm immobilization prior to ICSI damages the sperm plasma membrane, that (ii) this damage is sufficient for thiol-reducing agents to gain access to the sperm nucleus, and finally that (iii) PVP possibly interferes with sperm nucleus decondensation.
Asunto(s)
Fertilización In Vitro/métodos , Espermatozoides/ultraestructura , Membrana Celular/metabolismo , Membrana Celular/ultraestructura , Permeabilidad de la Membrana Celular , Núcleo Celular/ultraestructura , Citoplasma , Ditiotreitol , Eosina Amarillenta-(YS) , Femenino , Humanos , Masculino , Microinyecciones , Oocitos , Povidona , Espermatozoides/metabolismo , Coloración y EtiquetadoRESUMEN
We investigated the time course of human oocyte activation after intracytoplasmic sperm injection (ICSI) by observing the oocyte chromosome configuration at different times after injection. One day old human oocytes were injected with spermatozoa and subjected to cytogenetic analysis at 2, 3, 4 and 5 h after injection. We found that anaphase is initiated in the vast majority of the oocytes between 2 and 3 h after injection, and that by 4-5 h after injection most of the oocytes have reached the chromatin mass stage. Two distinguishable stages of sperm nucleus transformation were observed. The first phase-swelling-was reached within 2 h after the injection and was independent of oocyte activation. The second phase-the "brush'-like stage or decondensed chromatin stage-was found only in activated oocytes. Moreover, this stage was not reached before the chromatin mass stage (late telophase) of the oocyte. The same proportion of metaphase II oocyte chromosome configurations and unchanged sperm nuclei was found at any given time after injection. We conclude that: (i) ICSI allows users to obtain an almost synchronized population of activated oocytes; (ii) anaphase II is initiated in the majority of oocytes not later than 2-3 h after injection and telophase II is reached approximately 5 h after injection; and (iii) there are two distinguishable phases of sperm nucleus transformation after ICSI: oocyte activation-independent swelling of the sperm head and oocyte activation-dependent chromatin decondensation which is coupled to the beginning of oocyte chromosome decondensation.
Asunto(s)
Núcleo Celular/ultraestructura , Fertilización In Vitro/métodos , Oocitos/ultraestructura , Interacciones Espermatozoide-Óvulo/fisiología , Espermatozoides/ultraestructura , Cromosomas Humanos/ultraestructura , Citoplasma , Femenino , Humanos , Técnicas In Vitro , Masculino , Meiosis , Microinyecciones , Factores de TiempoRESUMEN
When intracytoplasmic sperm injection (ICSI) is performed, it is important to know the capacity of sperm cells to activate the oocytes, although knowledge of their ability to fuse with the oocytes is not vital. Hamster oocytes are not suitable for this purpose because they are easily activated by the injection procedure Itself. We therefore investigated whether mouse oocytes could be used to assess the activation properties of human spermatozoa. Mouse oocytes were randomized for injection with initially motile spermatozoa, medium, heat-treated or salt-extracted spermatozoa, and the survival and activation rates were examined. About half of the mouse oocytes survived the intracytoplasmic injection of a human sperm cell. Unlike hamster oocytes, the rate of activation provoked by the injection procedure itself was acceptably low (20%), resembling in this respect the behaviour of human oocytes. Following the injection of initially motile human spermatozoa, all mouse oocytes were activated. The injection of heat-treated or salt-extracted human spermatozoa resulted in activation rates of 14 and 15% respectively, comparable with the results following sham ICSI. These data support the hypothesis of a sperm-associated oocyte activation factor. In most activated oocytes, the human sperm nucleus decondensed to form a male pronucleus. Cytogenetic analysis at the first metaphase revealed that human sperm chromosomes were able to undergo replication in a heterologous environment.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Modelos Biológicos , Técnicas Reproductivas , Interacciones Espermatozoide-Óvulo/fisiología , Animales , Citoplasma , Femenino , Humanos , Cariotipificación , Masculino , Ratones , Microinyecciones , Oocitos/fisiología , Interacciones Espermatozoide-Óvulo/genética , Espermatozoides/fisiología , Espermatozoides/ultraestructuraRESUMEN
The aim of this study was to investigate whether the human spermatozoon participates in the activation of human oocytes following intracytoplasmic sperm injection (ICSI) and if so, by what mechanism. In the first series of experiments, we randomized human oocytes which had remained unfertilized after in-vitro fertilization (IVF) or ICSI, for intracytoplasmic injection with live spermatozoa, spermatozoa presumed to be dead and no spermatozoa. Secondly, unfertilized human oocytes and freshly ovulated mouse oocytes were randomized for intracytoplasmic and sub-zonal injection with human sperm cytosolic fraction (CF) before and after heat treatment. We found that oocyte injection with initially motile spermatozoa induces human oocyte activation at a significantly higher rate than injection with dead spermatozoa (61 versus 0%; P < 0.001) or injection without a spermatozoon (61 versus 14%; P < 0.001). Intracytoplasmic injection of CF activated both human and mouse oocytes at the same rate as sperm injection of human oocytes (activation rates of 70 and 65% respectively). This effect was greatly reduced by heat treatment of the CF. From these experiments we conclude firstly that the human spermatozoon injected intracytoplasmically contributes to human oocyte activation and secondly that the spermatozoon releases into the oocyte a heat-sensitive, intracellularly active factor, which is not species-specific.