RESUMEN
Neoscytalidium dimidiatum is an opportunistic fungus causing cutaneous infections mostly, which are difficult to treat due to antifungal resistance. In Malaysia, N. dimidiatum is associated with skin and nail infections, especially in the elderly. These infections may be mistaken for dermatophyte infections due to similar clinical appearance. In this study, Neoscytalidium isolates from cutaneous specimens, identified using morphological and molecular methods (28 Neoscytalidium dimidiatum and 1 Neoscytalidium sp.), were evaluated for susceptibility towards antifungal agents using the CLSI broth microdilution (M38-A2) and Etest methods. Amphotericin B, voriconazole, miconazole and clotrimazole showed high in vitro activity against all isolates with MIC ranging from 0.0313 to 1 µg/mL. Susceptibility towards fluconazole and itraconazole was noted in up to 10% of isolates, while ketoconazole was inactive against all isolates. Clinical breakpoints for antifungal drugs are not yet available for most filamentous fungi, including Neoscytalidium species. However, the results indicate that clinical isolates of N. dimidiatum in Malaysia were sensitive towards miconazole, clotrimazole, voriconazole and amphotericin B, in vitro.
Asunto(s)
Antifúngicos/farmacología , Ascomicetos/efectos de los fármacos , Dermatomicosis/microbiología , Ascomicetos/aislamiento & purificación , Humanos , Malasia , Pruebas de Sensibilidad MicrobianaRESUMEN
Candida wangnamkhiaoensis is a species clustered under the Hyphopichia clade has not ever been isolated from any clinical specimens. To the best of our knowledge, this is the first report of C. wangnamkhiaoensis associated with fungaemia in immunocompromised paediatric patient. The isolate was assigned a strain name as UZ1679/14, in which the identification was confirmed by a polymerase chain reaction-sequencing of the internal transcribed spacer (ITS) and large subunit (LSU) regions of the rRNA gene. Antifungal susceptibility pattern showed that the isolate was sensitive to anidulafungin, caspofungin, fluconazole and voriconazole. The patient clinically improved after the antifungal treatment with caspofungin.
Asunto(s)
Candida/aislamiento & purificación , Candidemia/microbiología , Huésped Inmunocomprometido , Anidulafungina , Antifúngicos/farmacología , Candida/clasificación , Candida/genética , Candida/ultraestructura , Candidemia/tratamiento farmacológico , Candidemia/inmunología , Caspofungina , Preescolar , ADN Espaciador Ribosómico , Equinocandinas/farmacología , Equinocandinas/uso terapéutico , Fluconazol/farmacología , Humanos , Hifa/aislamiento & purificación , Hifa/ultraestructura , Lipopéptidos/farmacología , Lipopéptidos/uso terapéutico , Masculino , Filogenia , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Voriconazol/farmacologíaRESUMEN
The number of new fungal pathogens is increasing due to growing population of immunocompromised patients and advanced identification techniques. Fereydounia khargensis is a yeast and was first described in 2014 from environmental samples. As far as we know, this is the first report of human infections associated with F. khargensis. The yeasts were isolated from blood of a HIV-positive patient and pleural fluid of chronic renal failure patient. Amplification and sequencing of the internal transcribed spacer and the large subunit regions confirmed the identity of the isolates. Both isolates showed multi-drug resistance to antifungal agents tested.
Asunto(s)
Basidiomycota/efectos de los fármacos , Basidiomycota/aislamiento & purificación , Farmacorresistencia Fúngica Múltiple , Micosis/diagnóstico , Micosis/patología , Adulto , Antifúngicos/farmacología , Sangre/microbiología , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Infecciones por VIH/complicaciones , Humanos , Masculino , Técnicas Microbiológicas , Microscopía , Persona de Mediana Edad , Micosis/microbiología , Derrame Pleural/microbiología , Insuficiencia Renal/complicaciones , Análisis de Secuencia de ADNRESUMEN
Trichosporon species are opportunistic yeasts which can cause infections, especially in immunocompromised patients. This is a report of Trichosporon ovoides that caused subcutaneous infection in a patient with underlying ischemic heart disease. The identification of fungal isolate was confirmed by PCR sequencing of ITS and large subunit regions in rRNA gene. In vitro susceptibility study showed that the isolate was susceptible to amphotericin B, fluconazole and voriconazole, and resistant to caspofungin, anidulafungin and itraconazole. The lesion improved after treatment with oral fluconazole and topical miconazole.
Asunto(s)
Antifúngicos/uso terapéutico , Dermatomicosis/tratamiento farmacológico , Huésped Inmunocomprometido , Infecciones Oportunistas/tratamiento farmacológico , Trichosporon/efectos de los fármacos , Tricosporonosis/tratamiento farmacológico , ADN Intergénico/genética , Dermatomicosis/microbiología , Femenino , Fluconazol/uso terapéutico , Humanos , Miconazol/uso terapéutico , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Infecciones Oportunistas/microbiología , Reacción en Cadena de la Polimerasa , Trichosporon/genética , Trichosporon/aislamiento & purificación , Tricosporonosis/microbiologíaRESUMEN
An 11-year-old girl presented with multiple blisters on her the right foot complicated with cellulitis. The conventional and molecular identification were performed on the culture. The internal transcribed spacer (ITS) region in rRNA gene of the isolate was amplified by PCR. The sequence of the amplified ITS region matched 99 % with that of Chaetomium globosum in the GenBank. This is the first report describing C. globosum causing cutaneous infection in Malaysia.
Asunto(s)
Celulitis (Flemón)/diagnóstico , Celulitis (Flemón)/patología , Chaetomium/clasificación , Chaetomium/aislamiento & purificación , Dermatomicosis/diagnóstico , Dermatomicosis/patología , Técnicas de Diagnóstico Molecular , Secuencia de Bases , Celulitis (Flemón)/etiología , Celulitis (Flemón)/microbiología , Chaetomium/genética , Niño , ADN de Hongos/química , ADN de Hongos/genética , ADN Espaciador Ribosómico/química , ADN Espaciador Ribosómico/genética , Dermatomicosis/complicaciones , Dermatomicosis/microbiología , Femenino , Humanos , Malasia , Técnicas Microbiológicas , Microscopía , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido NucleicoRESUMEN
Prototheca wickerhamii isolated from blood of 61-year-old kidney transplant patient was described. Although it is classified as an alga (genus Chlorella), the disease, protothecosis, is included under mycoses because of its clinical pathological presentations. Colony characteristics of P. wickerhamii are indistinguishable from other yeast-like organisms like Cryptococcus and Candida. Fortunately, commercial identification system for yeast can be used to identify this organism to the species level. Electron microscopy demonstrated "morula" or daisy-like appearance of its endosporulating sporangia. The organism was sensitive to amphotericin B by E test method. Even though human protothecosis is uncommon, it cannot be ignored because it is emerging as an opportunistic infection in immunosuppressed individuals. To our knowledge, this is the first reported case of disseminated algaemia due to P. wickerhamii in Malaysia.