Preparation of carboxyl-functionalized silica core-shell microspheres and their applications in weak cation exchange chromatography, heavy metal removal, and lysozyme enrichment.

Chromatography is a technique of separation based on adsorption and/or interaction of target molecules with stationary phases. Herein, we report the design and fabrication of BTDA@SiO2 core-shell microspheres as a new class of stationary phase and demonstrate its impressive performance for chromatographic separations. The silica microspheres of BTDA@SiO2 were synthesized by in situ method with 1,3,5-benzenetricarboxaldehyde and 3,5-diaminobenzoic to separate peptides and proteins on high-performance liquid chromatography. The BTDA@SiO2 core-shell structure has a high specific surface area and retention factor of 4.27 and 8.31 for anionic and cationic peptides, respectively. The separation factor and resolution were high as well. A typical chromatogram illustrated nearly baseline resolution of the two peptides in less than 3 min. The BTDA@SiO2 was also highly stable in the pH range of 1 to 14. Furthermore, the prepared BTDA@SiO2 core-shell material not only be used for chromatographic separation but also as heavy metal removal from water. Using a BTDA@SiO2, we also achieved a lysozyme enrichment with a maximum saturated adsorption capacity reaching 714 mg/g. In summary, BTDA@SiO2 has great application prospects and significance in separation and purification systems.

The Synthesis of Protein-Encapsulated Ceria Nanorods for Visible-Light Driven Hydrogen Production and Carbon Dioxide Reduction.

1D rare earth-based nanomaterials have attracted significant attention due to their excellent photo/electro-catalytic performance. The corresponding challenge is how to synthesize shape and size-controlled nanostructures in an easy scale-up way. Herein, the authors present a facile one-step strategy to design 1D multifunctional protein-encapsulated cerium oxide nanorods (PCNRs) by utilizing bovine serum albumin as an efficient biotemplate. Remarkably, the PCNRs exhibit high chemical and interfacial adhesion stability with intriguing properties, resulting in an exceptionally high activity towards H2 evolution and CO2 reduction. The photocatalytic activity of PCNRs to produce H2 is about 10 times higher than conventional CeO2 nanorods. The incorporation of rhodamine B into the PCNRs brings unprecedentedly high photocatalytic H2 evolution rate being 123 times higher than that of conventional CeO2 nanorods. Further the presence of the -NH2 groups on the PCNRs facilitated the adsorption and activation of CO2 and efficiently suppressed the proton reduction, and as a result, the PCNRs photocatalyst is highly active in converting CO2 to CO and CH4 , with the evolution rates being 50 and 83 times higher than those of conventional CeO2 nanorods, respectively. Achieving such efficient photocatalyst is a critical step toward practical production of high-value renewable fuels using solar energy.

Metal-Protein Hybrid Materials with Desired Functions and Potential Applications.

Metal nanohybrids are fast emerging functional nanomaterials with advanced structures, intriguing physicochemical properties, and a broad range of important applications in current nanoscience research. Significant efforts have been devoted toward design and develop versatile metal nanohybrid systems. Among numerous biological components, diverse proteins offer avenues for making advanced multifunctional systems with unusual properties, desired functions, and potential applications. This review discusses the rational design, properties, and applications of metal-protein nanohybrid materials fabricated from proteins and inorganic components. The construction of functional biomimetic nanohybrid materials is first briefly introduced. The properties and functions of these hybrid materials are then discussed. After that, an overview of promising application of biomimetic metal-protein nanohybrid materials is provided. Finally, the key challenges and outlooks related to this fascinating research area are also outlined.

Diacerein: Recent insight into pharmacological activities and molecular pathways.

Diacerein is a symptomatic slow-acting drug in osteoarthritis (SYSADOA) and the active metabolite is rhein. It is a non-steroidal anti-inflammatory drug with unique pharmacological properties as anti-oxidant and anti-apoptosis. Diacerein has recently shown to have a potential role by mediating anti-inflammatory as well as anti-oxidant and anti-apoptosis in kidney injury, diabetes mullites, and a beneficial effect on pain relief. It may have a therapeutic role in cancer, ulcerative colitis, testicular injury and cervical hyperkeratosis. Furthermore, diacerein has a valuable addition in combination therapy as a synergetic agent. This review, the first of its kind, highlights the proposed roles of diacerein in osteoarthritis and discusses recent results supporting its emerging roles with a particular focus on how these new insights may facilitate the rational development of diacerein for targeted therapies in the future.

Sn-Triggered Two-Dimensional Fast Protein Assembly with Emergent Functions.

The discovery of a general strategy for organizing functional proteins into stable nanostructures with the desired dimension, shape, and function is an important focus in developing protein-based self-assembled materials, but the scalable synthesis of such materials and transfer to other substrates remain great challenges. We herein tackle this issue by creating a two-dimensional metal-protein hybrid nanofilm that is flexible and cost-effective with reliable self-recovery, stability, and multifunctionality. As it differs from traditional metal ions, we discover the capability of Sn2+ to initiate fast amyloid-like protein assembly (occurring in seconds) by effectively reducing the disulfide bonds of native globular proteins. The Sn2+-initiated lysozyme aggregation at the air/water interface leads to droplet flattening, a result never before reported in a protein system, which finally affords a multifunctional 2D Sn-doped hybrid lysozyme nanofilm with an ultralarge area (e.g., 0.2 m2) within a few minutes. The hybrid film is distinctive in its ease of coating on versatile material surfaces with endurable chemical and mechanical stability, optical transparency, and diverse end uses in antimicrobial and photo-/electrocatalytic scaffolds. Our approach provides not only insights into the effect of tin ions on macroscopic self-assembly of proteins but also a controllable and scalable synthesis of a potential biomimic framework for biomedical and biocatalytic applications.